Team:BUCT-China/Contribution

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• Propagate iGEM
• Design new parts
• Experiment design

| Propagate iGEM |

1

This year, we are committed to spreading the idea of iGEM. We want to let more people understand iGEM through our work. We hope that more people will pay attention to the development of synthetic biology.Our official account platform, which contains knowledge about synthetic biology and our project background, reaches a maximum of 1000+ per day.

2

This year, through in-depth communication with the high school team, we participated in the online exchange meeting and shared our wonderful experience of participating in iGEM. It can make more senior high school teams have a deeper understanding of iGEM, and make them more interested in synthetic biology.

3

We carry out educational activities to a wider group through live network. We prepared a live broadcast about what iGEM is? What is synthetic biology? What is microplastics?. Through the Internet, to more people, not only to schools. Our live broadcast effect is very good, through the barrage and comments we communicate with the audience, we think this is a very meaningful thing.

Through these activities, in addition to iGEM and synthetic biology, we hope that through our work we can bring more people's attention to environmental issues and make more people realize that there are many people using biological knowledge to change our world.

| Design new parts |

Although this year's crown virus did cause a lot of difficulties for us to enter the laboratory, we still helped to design our part through some documents and proved the practicability of our part through a part of the verification experiments.
See the links in parts for details：
http://parts.igem.org/Part:BBa_K3664006
http://parts.igem.org/Part:BBa_K3664010
http://parts.igem.org/Part:BBa_K3664024

BBa_K3664006

This part contains two target genes: ACS2 (long chain acyl CoA synthase) and WS2 (wax ester synthase).It could express two proteins, CoA ligase and acyltransferase.The function of the two proteins as follows. CoA ligase:R1-COOH+CoA=R1-CO-S-CoA; acyltransferase:R1-CO-S-CoA+R2-OH=R1-COOR2.

BBa_K3664010

This part contains two genes: ppfadD and WS / DGAT. Between the two genes, there is a terminator of ppfadD gene and a promoter of WS / DGAT gene. At the same time, these two parts are connected by the way of tail enzyme connection, which is homocaudal enzyme (XbaI and NheI).The two genes expressed acyl coenzyme A synthetase and wax ester synthase.

BBa_K3664024

This part is a sequence designed by us for the surface display of Bacillus subtilis. We provide the CotG gene sequence of the anchor protein CotG and an unpliable linker peptide.In use, we should construct such a structure as promoter sequence, our part, and target gene (removing termination codon). We can also add a fluorescent protein EGFP to help us verify.In our experimental design, we constructed the plasmid pDG1730-pCotG-CotG-yifH-EGFP.

| Experiment design and verification |

We have designed a feasible surface display system for Bacillus subtilis spores, which can immobilize our target oxidase on the spore surface and achieve the biodegradation of PE while immobilizing the enzyme. Although the degradation efficiency of this enzyme is still not optimistic in previous studies, this provides us with a good idea.In the second part, we also constructed a complete metabolic pathway that can reuse the degradation products of PE (C14-16) to synthesize new materials.
See the link for details：
https://2020.igem.org/Team:BUCT-China/Engineering