Team:Baltimore BioCrew/Attributions
Attributions
-
Contributions arrow_downward
Team members Angelique Allen, Adam Hoffensetz, and Sam Ferraro designed experiments to measure how iron concentration affects growth of Synechococcus CB0101.
Angelique Allen, Maria Lyons, and Sam Ferraro setup experiments in their homes to measure the cyanobacteria growth.
Sam Ferraro conducted experiments to correlate different types of measurements (Secchi depth, optical density, and cell count.)
Adam Hoffensetz and Angelique Allen analyzed the cyanobacteria growth data to convert measurement units and calculate doubling time.
Daniel Fucich, a graduate student at University of Maryland Center for Environmental Science (UMCES), helped us improve our protocol for growing our cyanobacteria by advising the team on the best light intensity and temperature for Synechococcus CB0101.
Dr. Feng Cheng at The Institute of Marine and Environmental Technology (IMET) at UMCES provided us with a starting culture of Synechococcus CB0101.
Our PI, Dr. Lisa Schiefele, and the Baltimore Underground Science Space provided lab space, reagents, safety training, and equipment.
Our mentors, Breanna Takacs and Kalen Clifton, gave guidance to the Characterization team regarding experimental design, data analysis, and scientific writing.
-
Engineering arrow_downward
Team members Zoe Hsieh, Maria Lyons, Josh Dayie, Max Swann, and Harry Wojcik used the Ahlgren paper to choose potential genes for the project. The genes selected were involved in iron-related cellular processes.
Zoe Hsieh, Maria Lyons, Max Swann, and Harry Wojcik codon optimized the selected genes for Synechococcus sp. CB0101.
Our PI, Dr. Lisa Schiefele, and the Baltimore Underground Science Space provided lab space, reagents, safety training, and equipment.
Gabe Browning, a graduate student at the University of Florida, and Dr. Allen Place of the Institute of Marine and Environmental Technology helped with project support and advice.
Our mentors, Andy Johnston, Dr. Nina Rajpurohit, and Guoyue Xu, provided guidance in the New Part group’s research, experimental design, and organization.
-
Improved Part arrow_downward
Team members Alex Misiaszek, Avipsa Hamal, Heaven Cross, Kayla Le, Nathan Dayie, and Shubhan Bhat designed and synthesized variations to the iron-sensitive promoter system created by the ECUST 2018 iGEM team. These students are currently testing whether these promoters are more sensitive to lower iron concentrations.
Our PI, Dr. Lisa Schiefele, and the Baltimore Underground Science Space provided lab space, reagents, safety training, and equipment.
Our mentor, Wangui Mbuguiro, provided guidance to the team regarding experimental design, data analysis, and scientific writing.
-
Modeling arrow_downward
Team members Nathan Dayie, Max Swann, and Maria Lyons have modeled iron-limited cell growth using differential equations-based modeling in Matlab. They have used their models to estimate parameters for cyanobacteria in a previously published study (Patel, Sundaram, Patel, Kalra, 2017) using non-linear optimization techniques. These students have analyzed the results of their modeling to identify ways to improve their own study of cyanobacteria growth.
The team received Matlab licenses through the Mathworks grant to project teams competing in iGEM.
Our PI, Dr. Lisa Schiefele, and the Baltimore Underground Science Space provided lab space, reagents, safety training, and equipment.
Our mentors, Andy Johnston and Wangui Mbuguiro, have assisted the students in learning concepts of mathematical modeling in biology, programming in Matlab, analyzing model results, and scientific writing.
-
Human Practices arrow_downward
Team members Heaven Cross and Kayla Le performed researched for and designed social media posts for Instagram and Facebook to promote the project, increase outreach, and educate the audience on synthetic biology.
Shantika Bhat and Josh Dayie organized interviews with experts and stakeholders.
-
Collaboration arrow_downward
Team member Alex Misiaszek collaborated in the initiative hosted by the iGEM team of Maastricht University in the Netherlands to create a proceedings journal for iGEM 2020 by writing a 1500 words research article describing our project and our approach.
Alex Misiaszek designed a class with CLS CLSG UK iGEM team targeting middle-school students in order to raise awareness about anthropogenic effects on aquatic ecosystems.
Alex Misiaszek and Maria Lyons worked with iGEM Pitt in Pittsburgh, PA to record a podcast describing our work.
Our mentors, Guoyue Xu and Breanna Takacs, provided guidance on the writing and revising of the Journal Initiative and in the development of the Aquatic Ecosystems Class Collaboration.
-
Design arrow_downward
Team members Shubhan Bhat and Adam Hoffensetz drew designs for the pages in Wiki and created the T-shirt Design and poster design. All designs are original works that are inspired by Nickelodeon's SpongeBob Squarepants but are significant modifications of the original designs and characters.
Our mentor Breanna Takacs served as the project advisor.
-
Wiki arrow_downward
Team members Zoe Hsieh, Maria Lyons, Sam Ferraro, and Harry Wojcik created and formatted different pages for the 2020 Baltimore BioCrew team.
The wiki from Wageningen_UR 2019 iGEM team, which was developed with the intention of being a resource to other teams, was used as the template for our wiki. We also utilized their guide on the basics of HTML and web design.
Our mentor Kalen Clifton served as the project advisor.
-
Presentations arrow_downward
Team members Sam Ferraro, Nathan Dayie, Heaven Cross, Kayla Le, Shubhan Bhat, Zoe Hsieh, Angelique Allen, and Avipsa Hamla created the promotional video.
Adam Hoffensetz and Maria Lyons presented at the MidAtlantic Meetup hosted by the William & Mary iGEM team.