Team:GreatBay SZ/Part Collection
Index
Introduction
We developed a BIOT toolbox, which includes the basic genes collection for a complete BIOT-WildType system, as well as various mutated RBSs and pilin genes. The collection of RBSs consists of parts with different strengths, ranging from twice to one hundred times stronger than the original. It can be used to further explore the optimized combination that yields the most protein nanowires. The 17 mutated pilin genes can be helpful when the output voltage of the protein nanowire needs to be tuned in any future experiment. By making use of this toolbox, different mutated RBSs and pilin genes can be combined randomly to create new modified strains. The following is the parts included in our collection:
| Part Name | Part Code |
|---|---|
| PpdD Signal | BBa_K3596000 |
| Pilin Gene | BBa_K3596001 |
| hofB RBS | BBa_K3596002 |
| hofB+hofC | BBa_K3596003 |
| hofM RBS | BBa_K3596004 |
| hofM+hofN+hofO+hofP+hofQ | BBa_K3596005 |
| ppdA RBS | BBa_K3596006 |
| ppdA+ppdB+ygdB+ppdC+gspO | BBa_K3596007 |
| ppdA RBS mut1 | BBa_K3596008 |
| ppdA RBS mut2 | BBa_K3596009 |
| hofB RBS mut1 | BBa_K3596010 |
| hofB RBS mut2 | BBa_K3596011 |
| hofM RBS mut | BBa_K3596012 |
| Pilin Gene mut 1A | BBa_K3596013 |
| Pilin Gene mut 1B | BBa_K3596014 |
| Pilin Gene mut 1C | BBa_K3596015 |
| Pilin Gene mut 1D | BBa_K3596016 |
| Pilin Gene mut 2A | BBa_K3596017 |
| Pilin Gene mut 2B | BBa_K3596018 |
| Pilin Gene mut 2C | BBa_K3596019 |
| Pilin Gene mut 2D | BBa_K3596020 |
| Pilin Gene mut 3A | BBa_K3596021 |
| Pilin Gene mut 3B | BBa_K3596022 |
| Pilin Gene mut 3C | BBa_K3596023 |
| Pilin Gene mut 3D | BBa_K3596024 |
| Pilin Gene mut 4A | BBa_K3596025 |
| Pilin Gene mut 4B | BBa_K3596026 |
| Pilin Gene mut 4C | BBa_K3596027 |
| Pilin Gene mut 4D | BBa_K3596028 |
| Pilin Gene mut thin | BBa_K3596029 |