Team:NCTU Formosa
Overuse of carbon source results in lots of CO2 emissions which
contributes to worsening global warming. Besides, large scale biosynthesis in producing valuable
molecules and proteins come into its embryonic stage for more
sustainable and renewable ways of manufacturing and simultaneously faces multiple challenges in
bioengineering.
Therefore, we incorporate light-driven proton pump into non-phototrophic
bacteria. We designed a strain of E. coli that can use light and glucose as energy source
simultaneously, of which the concept is similar to a hybrid car, and thus we named it E. Hybrid.
E. Hybrid uses light as energy source to generate larger amounts of ATP for alleviating the metabolic stress caused by excessive reliance on glucose and supporting bioengineering reactions.
E. Hybrid uses light as energy source to generate larger amounts of ATP for alleviating the metabolic stress caused by excessive reliance on glucose and supporting bioengineering reactions.
We expressed functional Gloeobacter rhodopsin from ancient bacteria,
Gloeobacter violaceus, on E. coli Lemo21 and found it potent in creating alternative
proton motive force for oxidative phosphorylation and eventually proved it to be
ideal for improving protein and molecule yields.
This year, NCTU_Formosa attempted to make E. Hybrid serve as a fundamental strain that
uses light as energy sources, thus paving promising avenues for biosynthesis and creative daily
usages. To fulfill it, we provide a GR-expressing E.
coli culturing in our E. Hybrid-centered device which is equipped with parameters that are
modeled to be the optimized for E. Hybrid.
Team Promotion Video
More information
