Tomáš Kotačka¹, Barbora Hrnčířová ¹, Stanislav Juračka ¹,Petr Kohout ¹, Mgr. Karel Říha, Ph.D.², Mgr. Pavel Dvořák, Ph.D.², Ing. RNDr. Martin Marek, Ph.D.²
¹iGEM Student Team Member, ²iGEM Team Principal Investigators
Abstract: The goal of our project is to design a mobile device CYANOTRAP that degrades and detoxifies cyanobacterial blooms in lakes and water reservoirs. The core of the device are immobilized Bacillus subtilis that on their surface display enzymes required for the lysis of cyanobacteria cells and for degradation of cyanotoxins.
Such a design has several advantages over other solutions: 1) High mobility of the device would allow the targeting of the most infested areas, 2) Number of devices put in operation can easily be scaled according to the acreage of the water surface and degree of infestation, 3) Enzymatic lysis is more environmental friendly than chemical treatments, 4) The device can be used for constant surveillance and prevent formation of water blooms, 5) Use of bacteria as carriers of the enzymes provides longer sustainability as living bacteria can continuously replenish the enzymes.