The 2020 iGEM Evry Paris-Saclay team is composed of master's and bachelor's students, but the project would not have been possible without the help of many other people whom we wanted to thank.
### Host institutions
The Systems and Synthetic Biology Lab (iSSB) of the UMR8030 Genomics Metabolics headed by Patrick Wincker, hosted us. Jean-Loup Faulon kindly allowed us to carry out most of our experiments in his laboratory. We would like to thank the researchers and doctoral students for their accessibility and kindness, as well as for their help when we needed it and in particular to Sylvie Bobelet, Nazim Sarica and Laurent Jannière.
We thank Genopole, University of Evry and the University Paris-Saclay for providing the main financial support. We also thank the French Government Administration Grand Paris Sud who also provided valuable financial support.
### Material support
New England Biolabs (NEB) kindly supplied enzymes and kits for our molecular biology experiments.
SnapGene supplied free licences of its software for molecular biology which was essential for the design of our project.
IDT and Twist Bioscience offered DNA synthesis.
### Special thanks
A special thank to Ioana Popescu, who has encouraged the creation of our team and who has been there at every step of the project. In addition to being a supervisor she also provided help and emotional support to us. She supported our team leader in all the administrative tasks. The success of this project is largely due to her.
A special thanks to Manish Kushwaha too for his continuous help during the designing and modeling steps of the project. His knowledge of synthetic biology and computer sciences has been a real help for the team.
We thank Anna Niaraki who has helped us with the bioinformatics analyses as well as bibliographic research.
We thank Paul Soudier who helped us in the experimental work, especially with the cell-free experiments. He has been really helpful for understanding the implications of our results.
Lastly, we thank our advisor Sophia Belkhelfa for her help in the human practices part and in the laboratory over summer.
### External advice
We would especially like to thank the people who took the time to answer our questions and give us advice. We were fortunate enough to be able to speak with Annah Zhu, Assistant Professor at the Environmental Policy Group of Wageningen University and Research, Alex Widner, Professor of Plant Ecological Genetics at the Institute of Integrative Biology within the Department of Environmental Systems Science at ETH Zurich, Haïdar el-Ali, a well-known Senegalese environmentalist, and Julien Prieur, a legal expert in sustainable development.
### Distribution of the tasks
Maëva Cherriere is our versatile team leader, who created the team and took care of recruiting its members. While managing the administrative part of the project (financial aspects, search for sponsors and ordering of materials), she carefully coordinated the different parts of the team to allow the project to progress smoothly. In addition, she led the Human Practices work by establishing contact with professionals and organization of collaborations. Being in charge of the Communication department, she managed the different members of the team in this area, to publish on various social networks, to communicate with our sponsors.
Tom Zaplana, the team leader of iGEM Evry Paris-Saclay in 2019, helped Maëva a lot in starting and managing the team by passing on to her all the tips and tricks of the job.
### Wet lab
Doriane Blaise, Maxime Pispisa, Angelyne Saint-Julien, Mélissa Nguevo, Catia Goncalves, William Hamlet and Eva Gomes worked side by side to construct all the plasmids and perform the fluorescence measurements.
### Human Practices
Maëva Cherriere, our Education and Public Engagement enthusiastic leader, managed this part with an iron fist. She organized a virtual meetup with 4 other Parisian teams as well as the other different collaborations. With Micky-Love Mocombe, our sociologist, Alexandre Tardivel and Jellyssa Benjamin, the jurists of the team, we looked for and contacted the right people in order to get the various activities set up, attended meetings and explained our science to the public.
### Modeling and Wiki
Hassan Hijazi, Daniel Rodriguez-Pinzon and Tristan Reif-Trauttmansdorff took charge of the computational aspects of our project. Tristan Reif-Trauttmansdorff wrote the codes for our in-house toehold switch design pipeline, and the three of them have persevered and succeeded in processing the data generated by our measurement experiments.
Guillaume Coquard was our web designer. He created our team website and our wiki structure and he introduced the other members to HTML. He managed to support all the requests from our biologists, who often did not have the computer skills or language. He understood us nonetheless, and helped us with his skills in HTML, CSS, and JS. He also created a new team logo and co-created the project logo. Here are some of his works [Github](https://github.com/aemi-dev/).
Maëva Cherriere, together with Hassan Hijazi, Micky-Love Mocombe and Alexandre Tardivel created the promotion video.
The presentation video was coordinated by Maëva Cherriere with the contribution of Catia Goncalves, Hassan Hijazi, Tristan Reif-Trauttmansdorff and Daniel Rodriguez-Pinzon.
All the members have been involved in the brainstorming to find an interesting project. Additionally, Farah Martin and Bastien Mollet participated in the project at the beginning, but had to leave it before its completion. We thank them for their time.