# Part Collection
In our project, we developed biosensors based on toehold switches for sequence-based detection of rosewood, the most trafficked wildlife product in the world.
We employed several computational approaches to design and optimise the specificity of our biosensor towards the main magalasian rosewood species, Dalbergia maritima. In plants, DNA Barcoding makes use of three genes with enough variability to discriminate among species: MatK, RbcL and TrnL-UAA.
We designed and characterised a total of 18 toehold switches and their cognate triggers (BBa_K3453011 to BBa_K3453066).
To be able to use the molecular machinery of the common gut bacterium to sense the nucleic acid signatures specific to the Rosewood tree both in vivo (for the initial screening) as in vitro in a cell-free experimental setup (for the final product), all 18 toehold switches and their cognate triggers were equipped with a promoter and a terminator (BBa_K3453111 to BBa_K3453166).
To this collection, we added the "Full" DNA sequences of the three target genes of Dalbergia maritima MatK (BBa_K3453020), RbcL (BBa_K3453060) and TrnL-UAA (BBa_K3453060) and their corresponding transcription units (BBa_K3453120, BBa_K3453140 and BBa_K3453160 respectively).
