This year, we want to produce bioethanol from JUNCAO.We designed a universal integrated fermentation system that consists of three chassis organisms (E. coli&K.marxianus&Pichia pastoris) and the control elements that link them.
The biosensor-based self-regulation is realized by sensing the changes in the concentration of various intermediates (glucose,lactose and ethanol) during fermentation.We combine the reaction stages organically without interfering with each other.
In addition, we want to modify the fiber corporeal through rational design of proteins, so that it has the ability to break down the structure of cell wall, and finally achieve the effect of degrading lignin, cellulose and hemicellulose.
The main methods used in our model this time are introduced. One is logistic regression, also known as logistic regression analysis, which is mainly used in epidemiology. The more commonly used scenario is to explore the risk factors of a disease and predict a disease based on the risk factors. The probability of occurrence, etc.; the other is Response Surface Methodology, which uses reasonable experimental design methods and obtains certain data through experiments, and uses multiple quadratic regression equations to fit the functional relationship between factors and response values. Analysis is a statistical method to find the optimal process parameters and solve multivariate problems.
Our experimental project this year is the degradation and utilization of lignocellulose. In order to better integrate with social practice, we interviewed and consulted a large number of professors and experts, and we also investigated related companies. We are not satisfied with this, in order to better integrate with human life, we randomly interviewed 512 ordinary people and let them know about our project and synthetic biology.