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Team:IIT Roorkee/Parts



Basic Parts

We have introduced two new basic Parts to the iGEM registry as a contribution for us ebay future iGEM teams.

Part Number Name Description
Pseudomonas aeruginosa PAO1 R2 Pyocin Gene Cluster (tail fiber deleted) Gene cluster encoding for R-type pyocin with the tail fiber gene deleted
Pyocin-AP22 phage fusion tail fiber Fusion tail fiber of R pyocin tail fiber and Acinetobacter phage AP22 tail fiber

The first Part:BBa_K3564001 would be used for producing pyocins without any tail fiber. This is meant to be used in conjunction with Part:BBa_K3564002 or any others that are added in the K35640xx series, which are fusion tail fiber genes that would allow the pyocin to bind to a target bacterium.

We feel this contribution to the registry would allow future iGEM teams to conveniently incorporate pyocins into their systems, and target whichever bacterial species they should chose. The natural gene cluster sequence is highly complex and was not compatible for synthesis and also had 13 illegal restriction sites. All illegal restriction sites have been removed to make it compatible with both RFC10 as well as Type IIs assembly. It has also been sequence optimized for sequencing by changing codon usage to remove complexity.

Composite Parts

Part Number Name Description

Engineered Pyocin targeting Acinetobacter baumannii (with AP22 phage fusion tail fiber) Composite of BBa_K3564001 and BBa_K3564002 that expresses a Seekercin, i.e. a pyocin targeted to specifically Bind and kill Acinetobacter baumannii

Characterization for the composite parts would be updated once the experiments planned in the Wet Lab section are completed post-pandemic.