Project Description Design Contribution Implementation Engineering Success Results Notebook Software ClusteRsy Proof of Concept Lab Experiments Safety Parts Model Model People Team Members Attributions Collaborations Partnership Sponsors Human Practices Human Practices Science Communication Poster Poster Back to the top Design Table Basic parts Basic parts Down below is our basic part collection. All basic parts were designed to be able to assemble with BBa_K3182001 and our expression system, BBa_K3182200. To be able to assemble our basic parts with our cellulose binding domain (CBDcipA) we added a BamHI site inside the linker region of our construct, see Figure 1. The BamHI site was chosen because of it being a part of the RFC21, and coding for one glycine and one serine which is the end sequence of the thrombin cleavage site. The thrombin cleavage site was added into the linker region to be able to separate our fusion proteins. We had no trouble assembling our fusion proteins using the pink-white screening method (BBa_K3182100). Figure 1: The construct is composed of a carbohydrate binding domain (CBDcipA) with a GS-linker containing a thrombin (Th. CS) and BamHI site on the C-terminal (3'-end). The BamHI-site on the end of the linker allows exchange of fusion protein. The figure on the top is a picture of the construct in Benchling illustrating the CBD, GS-linker followed by a Th. CS and BamHI, ended by an agent which can be switched for any protein. The picture below is a graphical representation. Basic parts Table 2. Basic parts used in the wet lab part of our project. All basic parts used was designed to fit for assembly with BBa_K3182001. Biobrick ID Short name Function Size Designed by BBa_K3182001 CBDcipA Carbohydrate binding domain with a C-terminal linker 516 bp Oliver Hild Walett, Johan Larsson BBa_K3182003 PlyF307-SQ8C Acinetobacter genus lysin 444 bp Oliver Hild Walett BBa_K3182004 CHAP(K) Staphylococcus genus lysin 489 bp Leo Juhlin BBa_K3182005 LL-37 Antibacterial peptide 117 bp Oliver Hild Walett BBa_K3182006 Magainin 2 Antimicrobial peptide 75 bp Oliver Hild Walett BBa_K3182007 Pln1 Antibacterial peptide 117 bp Oliver Hild Walett BBa_K3182009 Parseqα Synthetic antimicrobial peptide 60 bp Johan Larsson, Jonatan Baggman BBa_K3182010 Parseqβ Synthetic antimicrobial peptide 60 bp Johan Larsson, Jonatan Baggman BBa_K3182013 mNeonGreen Fluorescent protein 771 bp Oliver Hild Walett, iGEM Linköping 2018
Table 2. Basic parts used in the wet lab part of our project. All basic parts used was designed to fit for assembly with BBa_K3182001.
