Team:NAU-CHINA/Parts text
Overview
We add IPTG in our culture medium to express CI protein to suppress PCI. So, there’s no switch-mazF(BBa_K3408003) expressed in Bacillus subtilis. Then we feed our earthworm with engineered bacteria.
When our engineered bacteria get into the intestinal tract, Pnar(BBa_K3408000) is activated by FNR without oxygen. phy(ycD)(BBa_K3408001), CI and trigger RNA(BBa_K3408002) could be expressed at the same time. CI protein would suppress PCI to avoid expressing switch-mazF. So, our engineered bacteria could play its role of fixing lead. With the intestinal tract moving, engineered bacteria would be excreted in earthworm’s faeces. Because of the presence of oxygen, Pnar would be suppressed and PCI would recover its activity. Then switch-mazF could be expressed and mazF could be released with the help of trigger RNA. Finally our engineered bacteria get to the end of their life.
Basic Parts
| Part Number | Name | Type |
|---|---|---|
| BBa_K3408000 | Pnar | Promoter |
| BBa_K3408001 | phy(ycD) | Coding |
| BBa_K3408002 | Trigger RNA | RNA |
| BBa_K3408003 | Switch RNA | RNA |
| BBa_K3408004 | Optimized cI | Coding |
| BBa_K3408005 | Optimized dPNI | Coding |
Composite Parts
| Part Number | Name | Type |
|---|---|---|
| BBa_K3408006 | Pnar-B0034-GFP-B0015 | Composite |
| BBa_K3408007 | Pnar-B0034-phy(ycD)-B0015 | Composite |
| BBa_K3408008 | Pnar-B0034-CⅠ-B0015-PCⅠ-B0034-GFP-B0015 | Composite |
| BBa_K3408009 | PliaG-trigger RNA-B0015-PCⅠ-switch RNA-GFP-B0015 | Composite |
| BBa_K3408010 | Pnar-trigger RNA-B0015-PCⅠ-switch RNA-mazF-B0015 | Composite |
| BBa_K3408011 | PliaG-B0034-lacI-Pgrac-B0034-CⅠ-PCⅠ-B0034-GFP-B0015 | Composite |
Contribution
We have found some new information about BBA_K 525515, BBA_K 1067006 and BBa_K1096001. We hope to help other teams to better understand the nature and characteristics of these parts and be able to use them more skillfully.
In order to better and more comprehensive understand our favorite reporter part whose name is BBa_E1010, this year NAU-CHINA uses SWISS MODEL (https://swissmodel.expasy.org/)to model and simulate the tertiary structure of protein. We hope that the prediction of the structure will help other teams to better understand the nature and characteristics of this part and be able to use the reporter gene more skillfully.
Learn more