Team:NCTU Formosa

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Overuse of carbon source results in lots of CO2 emissions which contributes to worsening global warming. Besides, large scale biosynthesis in producing valuable molecules and proteins come into its embryonic stage for more sustainable and renewable ways of manufacturing and simultaneously faces multiple challenges in bioengineering.
Therefore, we incorporate light-driven proton pump into non-phototrophic bacteria. We designed a strain of E. coli that can use light and glucose as energy source simultaneously, of which the concept is similar to a hybrid car, and thus we named it E. Hybrid.
E. Hybrid uses light as energy source to generate larger amounts of ATP for alleviating the metabolic stress caused by excessive reliance on glucose and supporting bioengineering reactions.
We expressed functional Gloeobacter rhodopsin from ancient bacteria, Gloeobacter violaceus, on E. coli Lemo21 and found it potent in creating alternative proton motive force for oxidative phosphorylation and eventually proved it to be ideal for improving protein and molecule yields.
This year, NCTU_Formosa attempted to make E. Hybrid serve as a fundamental strain that uses light as energy sources, thus paving promising avenues for biosynthesis and creative daily usages. To fulfill it, we provide a GR-expressing E. coli culturing in our E. Hybrid-centered device which is equipped with parameters that are modeled to be the optimized for E. Hybrid.
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