Team:NCTU Formosa/Notebook

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Description

Design

Hardware

Contribution

Engineering

Implementation

Sustainable

Safety

Experiment Results

Proof of Concept

Parts

Model

Human Practices

Science Communication

Team Introduction

Attributions

Collaborations

Partnership

Lab Note

Protocols

Medal Criteria

July 1

None

July 2

None

July 3

None

July 4

None

July 5

None

July 6

None

July 7

None

July 8

None

July 9

None

July 10

None

July 11

None

July 12

None

July 13

Digestion

Sample:pET32a, pUC57a GR(har)

Gel extraction

Sample:pET32a

Ligation

Sample:
- GR(har)+pET32a

Transformation

Sample:
- plasmid with GR(har) into DH5α

July 14

Colony PCR & gel electrophoresis

Sample: GR(har) transformation product

July 15

Culturing

July 16

Dilute IDT solutions

Sample:
- GR(har)

July 17

None

July 18

None

July 19

Colony PCR & gel electrophoresis

Sample:Lemo21, pET32a

Pfu PCR & gel electrophoresis

Sample: GR(har)

July 20

Culturing

July 21

Digestion

Sample:pET32a, GR(har)

Gel extraction

Sample:pET32a

Ligation

Sample:
- GR(har)+pET32a

Transformation

Sample:
- plasmid with GR(har) into DH5α

July 22

Pfu PCR & gel electrophoresis

Sample: GR(har)

Digestion

Sample: GR(har)

July 23

Ligation

Sample:
- GR(har)+pET32a

Transformation

Sample:
- plasmid with GR(har) into DH5α

July 24

Culturing

July 25

None

July 26

Transformation

Sample:
- plasmid pET32a into DH5α

July 27

Culturing

July 28

Digestion

Sample:pET32a

Ligation

Sample:
- GR(har)+pET32a

July 29

Transformation

Sample:
- plasmid with GR(har) into DH5α

July 30

Culturing

Digestion

Sample:pET32a

Ligation

Sample:
- GR(har)+pET32a

Transformation

Sample:
- plasmid with GR(har) into DH5α

PCR & gel electrophoresis

Sample:pET32a, GR(har)

July 31

None

August 1

None

August 2

Digestion

Sample:pET32a, GR(har)

August 3

Digestion

Sample:pET32a, GR(har)

Gel extraction

Sample:pET32a

Ligation

Sample:
- GR(har)+pET32a

Transformation

Sample:
- plasmid with GR(har) into DH5α

August 4

None

August 5

Culturing

August 6

None

August 7

None

August 8

None

August 9

None

August 10

None

August 11

Transformation

Sample:
- plasmid pET32a into Lemo21

August 12

Culturing

August 13

None

August 14

None

August 15

Funtional test

Sample:
- GR(har) in LB culture

August 16

None

August 17

Taiwan conference

August 18

Taiwan conference

Culturing

August 19

None

August 20

Funtional test

Sample:
- GR(har) in LB culture

August 21

None

August 22

None

August 23

None

August 24

Culturing

Transformation

Sample:
- plasmid with BBa_K274200 into Lemo21

August 25

Culturing

Transformation

Sample:
- plasmid pSB1A3 into Lemo21

Funtional test

Sample:
- GR(har) in LB culture

August 26

Culturing

M9 minimal medium preparation

August 27

Cell disruption

Sample:
- plasmid pSB1A3 in Lemo21
- plasmid pSB1A2 with BBa_K274200 in Lemo21

August 28

Funtional test

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium + Hydrogen peroxide
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium + pSB1A3 Lysate
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium + pSB1A2 BBa_K274200 Lysate

August 29

None

August 30

None

August 31

None

September 1

Functional test

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium + solvent(5% DMSO+PBS)
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium + pSB1A3 Lysate
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium + pSB1A2 BBa_K274200 Lysate
- plasmid pET32a with GR(har) in Lemo21 in LB broth + solvent(5% DMSO+PBS)
- plasmid pET32a with GR(har) in Lemo21 in LB broth + pSB1A3 Lysate
- plasmid pET32a with GR(har) in Lemo21 in LB broth + pSB1A2 BBa_K274200 Lysate
- plasmid pET32a in Lemo21 in LB broth + solvent(5% DMSO+PBS)
- plasmid pET32a in Lemo21 in LB broth + pSB1A3 Lysate
- plasmid pET32a in Lemo21 in LB broth + pSB1A2 BBa_K274200 Lysate
- plasmid pET32a in Lemo21 in LB broth
- plasmid pET32a in Lemo21 in M9 minimal medium
- plasmid pET32a with GR(har) in Lemo21 in LB broth
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium

September 2

None

September 3

None

September 4

None

September 5

None

September 6

None

September 7

Functional test

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium with yeast extraction+ pSB1A2 BBa_K274200 Lysate
- plasmid pET32a in Lemo21 in M9 minimal medium with yeast extraction + pSB1A2 BBa_K274200 Lysate
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium+ pSB1A2 BBa_K274200 Lysate
- plasmid pET32a in Lemo21 in M9 minimal medium + pSB1A2 BBa_K274200 Lysate

September 8

None

September 9

Functional test

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

September 10

None

September 11

None

September 12

Functional test

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

Glucose measurement

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

September 13

None

September 14

None

September 15

None

September 16

Glucose measurement

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

September 17

Glucose measurement (with IPTG　induction)

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

September 18

None

September 19

None

September 20

None

September 21

None

September 22

None

September 23

None

September 24

None

September 25

None

September 26

None

September 27

Glucose measurement (with IPTG　induction)

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

Functional test (with IPTG　induction)

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

Proton pump measurement (with IPTG　induction)

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

September 28

Functional test (with IPTG　induction and NaN₃)

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

September 29

None

September 30

None

October 1

None

October 2

None

October 3

None

October 4

None

October 5

None

October 6

None

October 7

None

October 8

None

October 9

None

October 10

None

October 11

Functional test (with IPTG　induction and NaN₃)

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

RFP expression in GR expression system (with IPTG　induction and NaN₃)

Sample:
- plasmid psB1K3 with GR(har) in Lemo21 in LB broth
- plasmid psB1K3 in Lemo21 in LB broth

October 12

None

October 13

None

October 14

None

October 15

Funtional test-Growth measurement

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

October 16

Funtional test-Growth measurement

Sample:
- plasmid pET32a with GR(har) in Lemo21 in M9 minimal medium
- plasmid pET32a in Lemo21 in M9 minimal medium

October 17

None

October 18

None

October 19

None

SUN	MON	TUE	WED	THU	FRI	SAT
			1	2	3	4
5	6	7	8	9	10	11
12	13	14	15	16	17	18
19	20	21	22	23	24	25
26	27	28	29	30	31

SUN	MON	TUE	WED	THU	FRI	SAT
						1
2	3	4	5	6	7	8
9	10	11	12	13	14	15
16	17	18	19	20	21	22
23	24	25	26	27	28	29
30	31

SUN	MON	TUE	WED	THU	FRI	SAT
		1	2	3	4	5
6	7	8	9	10	11	12
13	14	15	16	17	18	19
20	21	22	23	24	25	26
27	28	29	30

SUN	MON	TUE	WED	THU	FRI	SAT
				1	2	3
4	5	6	7	8	9	10
11	12	13	14	15	16	17
18	19	20	21	22	23	24
25	26	27	28	29	30	31