Team:NYU Abu Dhabi/Documentation/DOCS 20ee279bfcdc46b09c4fb108851b2757/Engineering 8297e0cf88af4f8fbfe00ad598c6a52f/Bio-Requirements a0409b5140a944dc8b04a55669a38016

Bio-Requirements

Bio-Requirements

  • Working Protocol
    • Sample
      • eDNA
      • Swab
    • DNA Extraction and Purification
      • Lysis Buffer
      • Bead Beating
    • Amplification
      • RPA/LAMP
      • Heating (Thermocycling?)
    • Reporting
      • If Cas 12 → Quench Single stranded DNA Reporters
      • If Cas 13 →Quench Single stranded RNA Reporters
      • Fluorescence
      • Lateral Flow Readout using FAM-Biotin Reporter
      • Electrochemical sensing

What we don't have right now:

  • Volumes of anything
  • Reaction temperatures
  • More definition on protocol options

Questions

NameCreatedTags
Sample Extraction & Input
Amplification & Detection
Communication of the Results