Team:QHFZ-China/Poster/2

Poster: QHFZ-China

Super-Serum for Engineered Bacteria

Presented by iGEM_20 QHFZ-China

Authors:

Poster design: Bohan Zhang^{1, 2}
Wiki code: Xinyuan Wang^{1, 2}
Content: Yixian Yang^{1, 2}
Proofread: Celine Siu Ham Zhang^{1, 2}
Instructor: Xing (Leaves) Zhang^{1, 3}

1. iGEM team QHFZ-China 2020, China, Asia.
2. Tsinghua University High School, Beijing, China.
3. Beijing ZENO Co., Ltd., Beijing, China.


Abstract

    Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of E. coli. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.

Team

    This is the second year for QHFZ-China to attend iGEM family. This year, QHFZ-China is composed of 15 students (Leaders: Yixian Yang, Zhandong Jiao; Members: Celine Siu Ham Zhang, Jiangshan Gao, Sizhe Duan, Marco Xue, Yeqi Wong, Xinyuan Wang, Bert Shan, James Haorui Wang, Junfei (Sophie) Li, Yiming Tang, Shao Chang, Siyuan Wang, Bohan Zhang), 6 advisors (Zhimeng Xu, Jun Ma, Aiqi Zhao, Li TianHong, Luo Shaowei, Weizhao Chen), 2 instructors (Xing (Leaves) Zhang, Tianze Zhu) and a primary PI (Shiqi Wang). All of us welcome your visiting our poster!

Introduction & Inspiration

    Our project is a continuation of the project last year. Last year, we made a portable household uric acid detector, whose core is a kind of genetically engineered bacteria. It would facilitate gout patients a lot. However, we realize that there are still some problems hindering its practical application. Among them, how to store the bacteria before use is an important issue.

    Engineered bacteria should be stored with a long enough shelf life before use. They should stay alive, while their metabolism should be paused. In laboratory, we use ultralow temperature to achieve this. That is why -80℃ refrigerator is necessary for molecular biology lab. However, it is hard such professional equipment out of laboratories, especially in our daily life. In general, household refrigerators can only achieve about -20℃, which is insufficient. Besides, it is not ideal to use the refrigerator, which we stored food, to store bacteria. Indeed, many engineered bacteria are designed for our daily life. We interviewed 10 iGEM teams and most of them believed that a suitable method to solve the problem would benefit the practical application of their products.

    As a result, we decide to come up with a biopreservation method, which could help the storage of engineered bacteria get rid of professional equipment.

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Super-Serum for Engineered Bacteria

Presented by iGEM_20 QHFZ-China

Authors:

Poster design: Bohan Zhang^{1, 2}
Wiki code: Xinyuan Wang^{1, 2}
Content: Yixian Yang^{1, 2}
Proofread: Celine Siu Ham Zhang^{1, 2}
Instructor: Xing (Leaves) Zhang^{1, 3}

1. iGEM team QHFZ-China 2020, China, Asia.
2. Tsinghua University High School, Beijing, China.
3. Beijing ZENO Co., Ltd., Beijing, China.


Abstract

    Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of E. coli. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.

Team

    This is the second year for QHFZ-China to attend iGEM family. This year, QHFZ-China is composed of 15 students (Leaders: Yixian Yang, Zhandong Jiao; Members: Celine Siu Ham Zhang, Jiangshan Gao, Sizhe Duan, Marco Xue, Yeqi Wong, Xinyuan Wang, Bert Shan, James Haorui Wang, Junfei (Sophie) Li, Yiming Tang, Shao Chang, Siyuan Wang, Bohan Zhang), 6 advisors (Zhimeng Xu, Jun Ma, Aiqi Zhao, Li TianHong, Luo Shaowei, Weizhao Chen), 2 instructors (Xing (Leaves) Zhang, Tianze Zhu) and a primary PI (Shiqi Wang). All of us welcome your visiting our poster!