Team:SZ-SHD/Implementation

Implementation

IMPLEMENTATION



In this section, we are going to introduce a standardized protocol to manufacture our bio-pesticide, the SWOT analysis of our product, and also our future development and business plan.


Production:

The engineered bacteria will be cultured in fermentor in factories, which then harvested and dried into powders and packed into products.

Storage:

Dry powders can be stored at room temperature or 4℃.

Using:

Dry powders can be activated with warm sugary water for 2 hours and diluted with water before use.




Swot analysis:



Strength:

1. Safe to use, our toxic proteins specifically target locusts, unlikely to damage mammalian cells.
2. Natural degradable, would not accumulates in food webs.
3. Fermentation is all that needs for manufacture, no expensive materials required.
4. Two toxins works together, locusts less likely to develop resistance.

Weakness:

1. Less lethal to pests than chemical pesticides.
2. Gradually lose effectiveness after released into environment.
3. Live bacteria needs to be stored in cool conditions.

Opportunities:

1. Public awareness of chemical pesticides that residues on vegetables, make our product more competitive with chemical pesticides.
2. Locusts have developed resistance against many chemical pesticides, these toxic proteins would be a potential alternative.
3. Government encourages people to use biopesticides, making it easier for us to develop some sponsorships.

Threat:

1. Transgenic organisms are unlikely to be approved to spray into the environment.
2. People may not obey the recommendations on our instruction booklets.
3. Misuse will lead to bacterial infections.






Instruction book:

Targeting market:

According to the results of questionnaire and interiviews:
Our pesticides would mainly benefits large agriculture production companies/institutes that.
1. Teach employees how to use pesticides properly.
2. Have suitable equipment to store the bacteria stock and culture them.
3. Are looking forward to find an biological alternative than chemical pesticides.






Future developing plan:

Primary:

1. Improve the SulAp-T4 circuit, with extra LexA gene rise the average inhibition level of SulAp and reduce leaky expression.
2. Study the activity of xncht released by UV-induced T4 lysis.
3. Study the xncht activity under different pH and temperature. A recommended test by prof. Zhang.
4. Assemble plasmid pSS9, ifchit with GFP tagged at C’ terminal. This can help us study whether the primary structure of ifchit was broken during translation. (if not, the purified fragment should emit green fluorescence).


Secondary:

1. Experiment on locusts, feed locusts with lysate supernatant containing Bt/ifchit1, (compare with control groups: boiled lysate, ddH2O, chemical pesticides) and record the LC50 (mortality) of each. This test was suggested by prof. Wang and prof. Zhang.

We got the idea of this new end goal from interviews with prof. Zhang:
1. Study the LC50 against locusts with different ages (or sizes)
2. Seal the toxin and lysis genes onto E. coli genome DNA to prevent horizontal gene transfer (contamination of gene pool.)




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