How can we keep our members and the environment safe?

Every member in SZ-SHD wet lab team has learned basic lab access and rules. Besides, we put personal biosecurity at high priority when working through our experiments. Working together with team OSA and Shanghai_SFLS_SPBS, we arrange members to be in charge of the daily cleaning of lab equipment and safety check several times a day. Our team set up detail rules towards equipment usages, waste management and specific procedures and protocols that help us maintain lab safety. Under the instruction of our advisors, we set up few penalty rules for members who did not follow basic lab safety, for the overall purpose of increasing the efficiency in lab safety management.

1. Which organisms, including viruses and cell lines, we used in our project?

E. coli DH5alpha; E. coli BL21DE3; E.coli DH10B

2. What risks could these organisms posed to our colleagues in the laboratory, or to community or the environment if they escape the lab?

Escherichia coli is a very common bacteria chassis in many iGEM projects. In general, E. coli does not hold too many risks. In order to reduce the leakage of bacteria in the environment, we set up a UV induced self-lysis switch in the bacteria. So that the escaping bacteria will activate the suicide process by ultraviolet light in the daytime.

3. How will we handle safety risks in our experiments?

- The PI and advisors of our team are responsible for the safety and security of our team. They offer lab security training for us and supervise us before entering the lab and doing the experiment. Also, they are all experienced working with microbiology, molecular biology, and biochemistry, which may help us a lot.
- The MSDS (Material Safety Data Sheet) of all the reagents we used will always be provided in the lab, every member of our team will be required to read them and strictly follow it for self-protection and the dispose protocol if they spilled out.

The Team Collaboration Special thanks Contribution Description Design Model Result Basic Composite Implementation Awards Judging Form Education Integrated HP Experiment Safety Lab Notebook Close About Us The Project Parts Judging Human Practices Lab