Ieva Lingytė, Eglė Vitkūnaitė, Auksė Kazlauskaitė, Austėja Sungailaitė, Edvinas Jurgelaitis, Emilija Radlinskaitė, Emilis Gaidauskas, Kamilė Liucija Vainiūtė, Paulius Sasnauskas, Liepa Šiupšinskaitė, Barbora Vasiliauskaitė, Monika Gineitytė, Denis Baronas, Povilas Šėporaitis, Rolandas Meškys, Paulius Toliušis
FlavoFlow: a comprehensive solution for exogenous fish infections detection, treatment and prevention.
Growing fish consumption rates encouraged marine culture farms to implement recirculating aquaculture systems that make intensive fish production compatible with environmental sustainability. Even if these systems reduce the use of terrestrial resources, water recirculation in such systems can cause significant losses because of bacterial or viral infections. A common pathogen of fish infections is the Flavobacterium genus bacteria, which can cause fish death in a few days after the initial infection. To detect the infection as soon as possible, we developed a rapid detection test based on helicase-dependent amplification and lateral-flow assay methods. Additionally, we created a novel treatment method which relies on a quorum sensing mechanism and exolysin protein with the aim of decreasing antibiotic consumption levels. Finally, to prevent forthcoming infections, our third goal is to provide a prevention system based on subunit vaccines encapsulated in alginate beads.