Difference between revisions of "Template:Virginia/scripts/seqDataJS"

 
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/*This file is used to store the text from the .gb files in a form that's readable by other scripts*/
 
/*This file is used to store the text from the .gb files in a form that's readable by other scripts*/
−
 
+
var sequences = {};
−
var sequences = {}
+
sequences.seq_2x_Res_6x_CoA = {};
−
 
+
sequences.seq_2x_Res_6x_CoA.varName = "seq_2x_Res_6x_CoA";
−
sequences.gBlockA = {};
+
sequences.seq_2x_Res_6x_CoA.name = "(2x Res 6x CoA)";
−
sequences.gBlockA.name = "gBlock A";
+
sequences.seq_2x_Res_6x_CoA.seqType = "both";
−
sequences.gBlockA.seqType = "linear";
+
sequences.seq_2x_Res_6x_CoA.comp = true;
−
sequences.gBlockA.data = `LOCUS      gBlock_A                995 bp ds-DNA    linear      02-SEP-2020
+
sequences.seq_2x_Res_6x_CoA.data = `LOCUS      (2x_Res,_6x_CoA)        3521 bp ds-DNA    circular    14-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    misc_binding        2668..2690
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    3089..3097
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    3078..3086
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    3034..3042
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Scar                2923..2930
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    misc_binding        2967..2989
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    gBlock              2501..2625
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2702..2710)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    3100..3108
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site 2744..2853
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(3410..3418)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Promoter            2931..2965
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(3421..3429)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    HIV-RT Binding Site join(3518..3521,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(3465..3473)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(3443..3451)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2436..2444
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    complement(3509..3517)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    3122..3130
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(3377..3385)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    gBlock              3187..3311
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    3023..3031
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    3045..3053
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(3487..3495)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Scar                3310..3317
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Zinc Finger Site    complement(2691..2699)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    2425..2433
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2414..2422
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    3001..3009
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(3498..3506)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    3012..3020
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(3454..3462)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Promoter            3318..3352
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Terminator          3241..3309
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(3399..3407)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Terminator          2555..2623
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(3432..3440)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(3388..3396)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    3056..3064
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    3067..3075
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Scar                2624..2631
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Terminator          2854..2922
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    3111..3119
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2990..2998
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Promoter            2632..2666
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2724..2732)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2735..2743)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    HIV-RT Binding Site 2445..2554
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    HIV-RT Binding Site 3131..3240
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(3476..3484)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2403..2411
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding        3354..3376
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2713..2721)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    gBlock              2800..2924
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
 +
    2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
 +
    2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
 +
    2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
 +
    2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
 +
    2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAAAAA ACGTGGCGCC
 +
    2761 CGAACAGGGA CGGATAGCTC AGTCGGTAGA GCATCAGACT TTTAATCTGA GGGTCCAGGG
 +
    2821 TTCAAGTCCC TGTTCGGGCG CCACGTTTTT TTTattagca gaaagtcaaa agcctccgac
 +
    2881 cggaggcttt tgactaaaac ttcccttggg gttatcattg ggtactagag TTGACGGCTA
 +
    2941 GCTCAGTCCT AGGTACAGTG CTAGCTGGAA TTGTGAGCGG ATAACAATTG AGGACGGCAT
 +
    3001 GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC
 +
    3061 TGGGATGTCG ATGCCATGCG GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
 +
    3121 TGCGGCTGGG AAAAAAAACG TGGCGCCCGA ACAGGGACGG ATAGCTCAGT CGGTAGAGCA
 +
    3181 TCAGACTTTT AATCTGAGGG TCCAGGGTTC AAGTCCCTGT TCGGGCGCCA CGTTTTTTTT
 +
    3241 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt
 +
    3301 atcattgggt actagagTTG ACGGCTAGCT CAGTCCTAGG TACAGTGCTA GCTGGAATTG
 +
    3361 TGAGCGGATA ACAATTCCCA GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT
 +
    3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
 +
    3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
 +
//`
 +
       
 +
sequences.seq_4x_Res_4x_CoA = {};
 +
sequences.seq_4x_Res_4x_CoA.varName = "seq_4x_Res_4x_CoA";
 +
sequences.seq_4x_Res_4x_CoA.name = "(4x Res 4x CoA)";
 +
sequences.seq_4x_Res_4x_CoA.seqType = "both";
 +
sequences.seq_4x_Res_4x_CoA.comp = true;
 +
sequences.seq_4x_Res_4x_CoA.data = `LOCUS      (4x_Res,_4x_CoA)        3521 bp ds-DNA    circular    14-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Zinc Finger Site    3089..3097
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2779..2787)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    complement(3421..3429)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    3122..3130
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          2599..2667
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site    2447..2455
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    3133..3141
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Terminator          2942..3010
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    3155..3163
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(3454..3462)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2757..2765)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    complement(3465..3473)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    misc_binding        3055..3077
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    misc_binding        2712..2734
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    gBlock             53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Scar                2668..2675
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    3111..3119
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(3498..3506)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    HIV-RT Binding Site join(3518..3521,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2469..2477
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Scar                3354..3361
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    complement(2768..2776)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Promoter            3362..3396
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2735..2743)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2414..2422
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    3144..3152
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2812..2820)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Promoter            3019..3053
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(3509..3517)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    HIV-RT Binding Site 3175..3284
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2790..2798)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    3078..3086
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Terminator          3285..3353
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(3432..3440)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2403..2411
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding        3398..3420
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Scar                3011..3018
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    gBlock              3231..3355
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2436..2444
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    complement(3443..3451)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    complement(2823..2831)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site    complement(3476..3484)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    gBlock              2545..2669
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2480..2488
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Zinc Finger Site    complement(2801..2809)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Promoter            2676..2710
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(3487..3495)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    3100..3108
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site 2489..2598
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    gBlock              2888..3012
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site 2832..2941
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2746..2754)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2425..2433
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2458..2466
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    3166..3174
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
 +
    2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
 +
    2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
 +
    2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
 +
    2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
 +
    2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
 +
    2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
 +
    2821 ATGCCGTCCT CAAAAAAAAC GTGGCGCCCG AACAGGGACG GATAGCTCAG TCGGTAGAGC
 +
    2881 ATCAGACTTT TAATCTGAGG GTCCAGGGTT CAAGTCCCTG TTCGGGCGCC ACGTTTTTTT
 +
    2941 Tattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt
 +
    3001 tatcattggg tactagagTT GACGGCTAGC TCAGTCCTAG GTACAGTGCT AGCTGGAATT
 +
    3061 GTGAGCGGAT AACAATTGAG GACGGCATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
 +
    3121 TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGAAAAAA
 +
    3181 AACGTGGCGC CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG
 +
    3241 AGGGTCCAGG GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa
 +
    3301 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga
 +
    3361 gTTGACGGCT AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT
 +
    3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
 +
    3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
 +
//`
 +
       
 +
sequences.seq_Addgene_109383_pSB1C3Lux = {};
 +
sequences.seq_Addgene_109383_pSB1C3Lux.varName = "seq_Addgene_109383_pSB1C3Lux";
 +
sequences.seq_Addgene_109383_pSB1C3Lux.name = "(Addgene 109383) pSB1C3-Lux";
 +
sequences.seq_Addgene_109383_pSB1C3Lux.seqType = "both";
 +
sequences.seq_Addgene_109383_pSB1C3Lux.comp = true;
 +
sequences.seq_Addgene_109383_pSB1C3Lux.data = `LOCUS      (Addgene_#109383)_pSB1C 9707 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_109383_206254"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    terminator      1..58
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    rep_origin      complement(253..841)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      1024..1118
 +
                    /label="lambda t0 terminator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    promoter        2955..3239
 +
                    /label="araBAD promoter"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    terminator      1982..2025
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2028..2049
 +
                    /label="BioBrick prefix"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    CDS            complement(1139..1798)
 +
                    /label="CmR"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    primer_bind    complement(82..99)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    join(9687..9707,1..2049)
 +
                    /label="pSB1C3 Backbone Region"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    source          1..9707
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind    1960..1978
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    CDS            complement(2050..2928)
 +
                    /label="araC"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    primer_bind    complement(333..352)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind    3162..3181
 +
                    /label="pBAD Forward"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    9687..9707
 +
                    /label="BioBrick suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    9647..9650
 +
                    /label="Overhang for end of pduD part"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind    1713..1732
 +
                    /label="CAT-R"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    primer          complement(2062..2093)
 +
                    /label="pSB1C3 Reverse Primer"
 +
                    /note="sequence: ACTGATCGTCTCTgttgtgaagaaaaagtgaatgatgtagccgtc"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    primer          9647..9670
 +
                    /label="pSB1C3 Forward Primer"
 +
                    /note="sequence: TATGATCGTCTCAgtcagaacagatgtttgccgatgc"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
ORIGIN
 +
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
 +
      61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
 +
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
 +
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
 +
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
 +
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
 +
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
 +
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
 +
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
 +
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
 +
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
 +
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
 +
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
 +
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
 +
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
 +
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
 +
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
 +
    1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
 +
    1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
 +
    1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
 +
    1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
 +
    1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
 +
    1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
 +
    1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
 +
    1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
 +
    1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
 +
    1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
 +
    1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
 +
    1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
 +
    1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
 +
    1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
 +
    1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
 +
    1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
 +
    1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
 +
    2041 cttctagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
 +
    2101 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
 +
    2161 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
 +
    2221 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
 +
    2281 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
 +
    2341 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
 +
    2401 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
 +
    2461 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
 +
    2521 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
 +
    2581 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
 +
    2641 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
 +
    2701 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
 +
    2761 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
 +
    2821 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
 +
    2881 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
 +
    2941 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
 +
    3001 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
 +
    3061 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
 +
    3121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
 +
    3181 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
 +
    3241 cccgtttttt tgggctagca gggaaaataa tgaataaatg tattccaatg ataattaatg
 +
    3301 gaatgattca agattttgat aattatgcat ataaagaagt taaactaaat aatgataata
 +
    3361 gagtaaaatt atctgtcatt actgaaagtt cagtttcaaa aacattaaat atcaaagata
 +
    3421 gaattaatct aaatttaaat cagattgtga attttttata taccgttggt caacgatgga
 +
    3481 aaagtgaaga atataatcgg cgacgaacct atatccgtga gttaaaaaca tatcttggtt
 +
    3541 attctgatga aatggcaaga ttagaagcga attggattgc aatgttattg tgctctaaaa
 +
    3601 gtgctttgta tgacattgtt aattatgatt tgggctctat acacgtatta gatgaatggc
 +
    3661 ttccacgtgg tgattgctat gttaaagcac aaccgaaagg tgtttctgtt cacttgttag
 +
    3721 ctggtaatgt tccattatca ggagtgacat ctattttgcg tgctatttta acaaaaaatg
 +
    3781 agtgcattat taaaacttcg tcttcagatc cttttactgc aaacgcttta gtttccagtt
 +
    3841 ttattgatgt taatgcagac catccaataa ccaaatcaat gtctgttatg tattggccgc
 +
    3901 atgatgaaga tatgactcta tctcaaagaa taatgaatca tgccgacgtg gttattgctt
 +
    3961 ggggtggaga cgaggcgatt aaatgggcgg taaaatattc accaccgcat gtcgatattc
 +
    4021 tgaaatttgg accaaagaaa agcttaagta ttattgaagc tcctaaagat atagaagccg
 +
    4081 cagcaatggg ggttgctcat gatatttgtt tctatgacca gcaagcctgc ttctctactc
 +
    4141 aagacgttta ttatatagga gataatttac ctttattttt aaatgaactt gaaaaacagc
 +
    4201 tagatcgata cgcgaaaatt ttaccaaaag gttcaaatag ttttgatgaa aaagcggcgt
 +
    4261 ttactcttac tgaaaaagaa agtctatttg ctggatatga agtgagaaag ggagataagc
 +
    4321 aagcttggtt aatagtcgta tcacctacaa atagctttgg aaatcaaccg ctatcacgaa
 +
    4381 gtgtgtatgt tcatcaagta tctgatatta aagagataat tccttttgtt aataaaaata
 +
    4441 gaacacaaac tgtttctatt tatccttggg aagcgtcatt aaaatatcga gataaattag
 +
    4501 caagaagtgg agttgaaaga attgttgaat caggcatgaa taatattttc agagttggag
 +
    4561 gggctcatga ttcattatct cctctccagt acctagttag gtttgtatcg catgagagac
 +
    4621 catttaatta tacgacaaaa gatgttgcgg ttgaaatcga acaaacacgt tacttagagg
 +
    4681 aagataagtt tttagttttt gtcccatagt taaaggaaat tatatgaaag atgaaagtgc
 +
    4741 tttttttacg attgatcaca tcatcaagct tgataatggt cagtctatcc gagtttggga
 +
    4801 aacactccct aaaaagaacg taccagagaa aaaacataca atacttattg cttcgggttt
 +
    4861 tgctagaaga atggatcatt ttgcaggtct tgctgagtat ttatctacta acggttttca
 +
    4921 tgtcattcgc tacgattctt tgcatcatgt tggattaagc agtggatgta taaatgaatt
 +
    4981 tacgatgtcg attggaaaaa atagcctgct tacagtcgta gattggctta cagatcatgg
 +
    5041 tgtcgaacga atagggctga ttgctgctag tttgtcagcg agaatcgcct atgaggtagt
 +
    5101 aaataaaatt aaattatcat ttttaattac ggccgtaggt gtcgttaatc ttagagatac
 +
    5161 attagaaaaa gcattggagt atgactattt gcaattacct atttcagagt taccagaaga
 +
    5221 tcttgacttt gaaggtcata atttaggatc ggaggtcttt gttacagatt gctttaaaca
 +
    5281 taattgggac acgttagact cgacacttaa tagtgttaaa ggattagcga ttccatttat
 +
    5341 tgcttttact gcaaacgatg atagttgggt aaagcaaagt gaagttatag agctcattga
 +
    5401 tagcattgaa tctagtaatt gtaagctcta ttcgctaatt ggaagttcac atgatcttgg
 +
    5461 ggaaaatttg gttgtattaa gaaattttta tcaatcagtg acgaaggcag ccttagcatt
 +
    5521 agatgatggt ttattggatt tagagataga cattattgaa cctcgatttg aggacgttac
 +
    5581 aagtattact gttaaggagc gtagattaaa aaatgaaatt gaaaatgaat tattagaatt
 +
    5641 ggcttaaata aacagaatca ccaaaaagga atagagtatg aagtttggaa atatttgttt
 +
    5701 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg
 +
    5761 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga
 +
    5821 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac
 +
    5881 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga
 +
    5941 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg
 +
    6001 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac
 +
    6061 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag
 +
    6121 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc
 +
    6181 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc
 +
    6241 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa
 +
    6301 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat
 +
    6361 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa
 +
    6421 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg
 +
    6481 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa
 +
    6541 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga
 +
    6601 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc
 +
    6661 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc
 +
    6721 tttcttaaaa gaacctaaat aaattactta tttgatacta gagataataa ggaacaagtt
 +
    6781 atgaaatttg gattattttt tctaaacttt cagaaagatg gaataacatc tgaagaaacg
 +
    6841 ttggataata tggtaaagac tgtcacgtta attgattcaa ctaaatatca ttttaatact
 +
    6901 gcctttgtta atgaacatca cttttcaaaa aatggtattg ttggagcacc tattaccgca
 +
    6961 gctggttttt tattagggtt aacaaataaa ttacatattg gttcattaaa tcaagtaatt
 +
    7021 accacccatc accctgtacg tgtagcagaa gaagccagtt tattagatca aatgtcagag
 +
    7081 ggacgcttca ttcttggttt tagtgactgc gaaagtgatt tcgaaatgga attttttaga
 +
    7141 cgtcatatct catcaaggca acaacaattt gaagcatgct atgaaataat taatgacgca
 +
    7201 ttaactacag gttattgtca tccccaaaac gacttttatg attttccaaa ggtttcaatt
 +
    7261 aatccacact gttacagtga gaatggacct aagcaatatg tatccgctac atcaaaagaa
 +
    7321 gtcgtcatgt gggcagcgaa aaaggcactg cctttaacat ttaagtggga ggataattta
 +
    7381 gaaaccaaag aacgctatgc aattctatat aataaaacag cacaacaata tggtattgat
 +
    7441 atttcggatg ttgatcatca attaactgta attgcgaact taaatgctga tagaagtacg
 +
    7501 gctcaagaag aagtgagaga atacttaaaa gactatatca ctgaaactta ccctcaaatg
 +
    7561 gacagagatg aaaaaattaa ctgcattatt gaagagaatg cagttgggtc tcatgatgac
 +
    7621 tattatgaat cgacaaaatt agcagtggaa aaaacagggt ctaaaaatat tttattatcc
 +
    7681 tttgaatcaa tgtccgatat taaagatgta aaagatatta ttgatatgtt gaaccaaaaa
 +
    7741 atcgaaatga atttaccata ataaaattaa aggcaatttc tatattagat tgccttttta
 +
    7801 aatttctgtt gatattaggt attactggag agggtatgac tgtccatact gaatataaaa
 +
    7861 gaaatcaaat cattgctagt tcagaaattg atgatcttat ctttatgacg aaaccacaag
 +
    7921 agtggtcatt tgaagagcaa aaagaaatac gggataaatt agttcgtgag gctttttatt
 +
    7981 ttcactacaa tagaaatgaa gaatatagaa attattgtat caatcagcat gtgagtgata
 +
    8041 atttacacac tattgatgaa atacccgtgt ttccaacatc tgtttttaaa tataagaaat
 +
    8101 tacatactgt cacagccgag gacattgaaa attggtatac aagtagtgga actcgtggag
 +
    8161 taaaaagtca tattgcacgt gatcgtctta gcattgaacg cttgcttggt tctgtcaact
 +
    8221 tcggaatgaa atacgttgga gattggtttg agcatcaaat ggaattgata aatttaggac
 +
    8281 cagatagatt caatacaaat aatatttggt ttaaatatgt catgagtttg gtcgagttac
 +
    8341 tttatccgac tgaatttaca gttgataatg acaaaataga ttttgaaaaa acagtaaaac
 +
    8401 atctatttag aattaagaat agtaaaaaag acatttgctt aattgggcca ccattttttg
 +
    8461 tgtatctttt gtgccaatat atgaaagaaa acaatattga atttaaagga ggagatagag
 +
    8521 tacatattat tactggtgga ggatggaaat ctaatcagaa tgactcttta gatcgtgctg
 +
    8581 attttaatca attattaatg gatactttcc aactcgacaa gattaatcaa attagagata
 +
    8641 cctttaatca agttgagctt aatacttgtt tttttgaaga tgaatttcaa agaaaacatg
 +
    8701 ttccaccgtg ggtatatgct cgggctcttg atcctgaaac cttgaaaccc gtagcagatg
 +
    8761 gtgagatcgg gttgttaagt tatatggacg cctcatcaac tgcttaccct gcttttattg
 +
    8821 ttactgatga tatcggtatt gtaaaagaaa ttagagaacc agatccttac ccaggggtaa
 +
    8881 ctgttgagat tgttcggcgc ttaaatacac gtgcgcaaaa aggatgcgcg ctctctatgg
 +
    8941 ctaatgtcat acaaaagaat atcaaggatt aagttatgat tgttgatggt agagtttcaa
 +
    9001 aaatagtatt agcgtcaata agaaataata tatataaggt atttattact gtaaattcac
 +
    9061 caataaagtt catcgctgga caatttgtaa tggtcacgat taatgggaaa aaatgccctt
 +
    9121 tttcaattgc gaattgcccg acaaaaaatt acgaaataga attgcatatt ggtagttcga
 +
    9181 atagagactg ctcattggat attatcgaat attttgtcga tgctcttgtt gaggaagtcg
 +
    9241 caattgagtt agatgctccc catggaaacg cttggttacg gtctgaaagt aataacccat
 +
    9301 tgctattaat tgcgggaggt acaggtttat catatataaa tagcattcta acaaattgct
 +
    9361 taaataggaa tatacctcaa gatatttatc tttactgggg agtaaaagac agttctcttt
 +
    9421 tgtatgaaga tgaagagtta ctaaacttat cactaaacaa caaaaacttt cattatattc
 +
    9481 ctgttattga agataaaagt gaagaatgga tagggagaaa aggcactgtt cttgatgctg
 +
    9541 tcatggaaga ttttactgat cttacttatt ttgatattta tgtttgtgga cccttcatga
 +
    9601 tggctaaaac agcaaaagaa aaattaattg aagagaaaaa agcaaagtca gaacagatgt
 +
    9661 ttgccgatgc ttttgcatac gtataatact agtagcggcc gctgcag
 +
//`
 +
       
 +
sequences.seq_4CL_Intro_gblock = {};
 +
sequences.seq_4CL_Intro_gblock.varName = "seq_4CL_Intro_gblock";
 +
sequences.seq_4CL_Intro_gblock.name = "4CL Intro gblock";
 +
sequences.seq_4CL_Intro_gblock.seqType = "linear";
 +
sequences.seq_4CL_Intro_gblock.comp = true;
 +
sequences.seq_4CL_Intro_gblock.data = `LOCUS      4CL_Intro_gblock        480 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    437..466
 +
                    /label="Spacer"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    misc_feature    40..73
 +
                    /label="BBa_J23102"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    misc_feature    122..436
 +
                    /label="ZIF268"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    98..111
 +
                    /label="BBa_J61115"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    Biobricking    18..39
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_binding    74..97
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 CGTCAGGCTC TTCACACgaa ttcgcggccg cttctagagt gacagctagc tcagtcctag
 +
      61 gtactgtgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGGGATA Ctaaatacat
 +
      121 gATGCACCAC CATCACCATC ATCCCGGTGA GAAACCATAT GCGTGCCCGG TGGAAAGCTG
 +
      181 TGATAGACGC TTTAGCCGTA GTGACGAACT GACCCGCCAC ATTCGAATAC ATACGGGGCA
 +
      241 AAAACCGTTT CAGTGCCGCA TTTGTATGCG TAACTTCTCA CGGAGTGATC ATTTAACCAC
 +
      301 TCATATTCGT ACTCATACAG GCGAAAAACC TTTTGCCTGC GACATCTGTG GTCGCAAATT
 +
      361 CGCACGTTCC GATGAGCGCA AGCGTCACAC GAAAATCCAT ACCGGTGGCG GGAGCGGCGG
 +
      421 CGGATCTGGT GGCTCGggtg gaagtggagg tggaagtgga ggtagtAGAA GAGCTGCAAG
 +
//`
 +
       
 +
sequences.seq_4CL_Outro_gblock = {};
 +
sequences.seq_4CL_Outro_gblock.varName = "seq_4CL_Outro_gblock";
 +
sequences.seq_4CL_Outro_gblock.name = "4CL Outro gblock";
 +
sequences.seq_4CL_Outro_gblock.seqType = "linear";
 +
sequences.seq_4CL_Outro_gblock.comp = true;
 +
sequences.seq_4CL_Outro_gblock.data = `LOCUS      4CL_Outro_gblock        137 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    stem_loop      15..66
 +
                    /label="T0 terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    Biobricking    67..87
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 TTCCGGGCTC TTCAttgttc agaacgctcg gtcttgcaca ccgggcgttt tttctttgtg
 +
      61 agtccatact agtagcggcc gctgcagGGA AGAAGAGCtc caagagagtg aagggatgtc
 +
      121 aagctgccat gAATTGA
 +
//`
 +
       
 +
sequences.seq_ACC_intro_gblock = {};
 +
sequences.seq_ACC_intro_gblock.varName = "seq_ACC_intro_gblock";
 +
sequences.seq_ACC_intro_gblock.name = "ACC intro gblock";
 +
sequences.seq_ACC_intro_gblock.seqType = "both";
 +
sequences.seq_ACC_intro_gblock.comp = true;
 +
sequences.seq_ACC_intro_gblock.data = `LOCUS      ACC_intro_gblock        2034 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    99..112
 +
                    /label="RBS"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    40..74
 +
                    /label="Promoter"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    113..1885
 +
                    /label="ACCalpha"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    1889..2017
 +
                    /label="Terminator"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_binding    75..98
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    18..39
 +
                    /label="Prefix"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
ORIGIN
 +
        1 AATGCTGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
 +
      61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGAGGTGAC ATATGAGCGT
 +
      121 GGAAACCCGT AAAATCACTA AAGTTTTGGT CGCGAACCGG GGTGAAATCG CTATTCGCGT
 +
      181 TTTTCGTGCG GCCCGCGATG AGGGTATCGG CAGTGTTGCT GTCTACGCCG AGCCGGATGC
 +
      241 CGATGCGCCA TTCGTGAGCT ATGCGGACGA AGCCTTTGCC CTGGGCGGCC AGACATCGGC
 +
      301 CGAATCGTAT CTGGTAATCG ACAAAATCAT TGACGCCGCT CGTAAGAGCG GGGCTGATGC
 +
      361 AATCCATCCC GGATACGGCT TCCTTGCAGA AAACGCGGAC TTCGCCGAAG CTGTTATAAA
 +
      421 CGAAGGGCTA ATTTGGATTG GCCCGTCCCC AGAGAGCATC AGATCCTTAG GAGACAAAGT
 +
      481 AACAGCGCGG CATATTGCGG ATACCGCAAA GGCGCCGATG GCACCCGGGA CGAAAGAGCC
 +
      541 CGTGAAAGAT GCCGCCGAAG TCGTGGCTTT TGCAGAGGAG TTTGGGCTGC CGATCGCTAT
 +
      601 CAAAGCAGCC TTTGGTGGTG GTGGCCGTGG AATGAAGGTG GCCTACAAAA TGGAAGAAGT
 +
      661 CGCGGATCTG TTTGAGTCTG CGACCCGTGA AGCGACCGCC GCTTTCGGTC GAGGTGAGTG
 +
      721 CTTTGTGGAA CGGTATCTGG ACAAAGCGCG CCACGTGGAA GCGCAAGTCA TCGCAGATAA
 +
      781 ACATGGAAAC GTCGTAGTGG CGGGCACCCG CGACTGCTCG CTTCAGCGCC GTTTTCAAAA
 +
      841 ACTCGTGGAA GAAGCGCCGG CACCGTTTCT CACTGATGAC CAAAGAGAGC GTTTACACTC
 +
      901 AAGTGCAAAG GCGATTTGTA AAGAAGCTGG CTATTATGGT GCTGGCACTG TTGAATACTT
 +
      961 GGTTGGTTCT GATGGACTCA TCTCGTTCCT GGAAGTGAAT ACCCGTCTGC AAGTCGAACA
 +
    1021 CCCTGTGACG GAAGAAACGA CAGGTATCGA CCTGGTTCGC GAAATGTTTC GTATTGCGGA
 +
    1081 AGGTCATGAA CTGTCTATTA AAGAAGATCC AGCACCCCGC GGCCACGCCT TCGAGTTCCG
 +
    1141 TATTAACGGC GAGGATGCCG GCTCAAACTT CATGCCGGCT CCTGGAAAGA TTACTAGCTA
 +
    1201 CCGAGAACCA CAGGGGCCTG GCGTTCGTAT GGACTCCGGC GTCGTGGAGG GGAGCGAAAT
 +
    1261 ATCTGGTCAG TTTGACAGTA TGTTGGCAAA GCTTATTGTC TGGGGCGATA CCCGCGAACA
 +
    1321 GGCCTTACAG CGCAGCCGTC GCGCCCTGGC GGAATATGTA GTGGAGGGTA TGCCGACCGT
 +
    1381 AATCCCGTTC CACCAGCATA TTGTGGAAAA TCCGGCCTTT GTTGGGAATG ATGAAGGTTT
 +
    1441 TGAAATTTAC ACCAAATGGA TAGAGGAAGT ATGGGACAAT CCTATTGCAC CTTATGTGGA
 +
    1501 TGCGTCCGAA CTGGATGAGG ATGAAGATAA AACGCCAGCG CAGAAAGTTG TTGTAGAAAT
 +
    1561 TAATGGCCGC CGCGTTGAGG TGGCGCTGCC GGGCGATCTG GCACTGGGCG GGACAGCGGG
 +
    1621 ACCGAAAAAA AAGGCTAAAA AACGGAGGGC GGGCGGTGCG AAAGCAGGCG TCAGTGGGGA
 +
    1681 TGCGGTAGCG GCACCGATGC AGGGTACGGT GATTAAAGTA AATGTCGAGG AAGGCGCAGA
 +
    1741 GGTGAACGAA GGTGATACCG TGGTTGTACT TGAAGCAATG AAGATGGAAA ACCCGGTGAA
 +
    1801 AGCCCATAAA TCAGGCACGG TGACGGGTTT GACCGTTGCC GCGGGTGAAG GCGTCAATAA
 +
    1861 AGGTGTGGTT CTGTTAGAGA TTAAATAAcc aggcatcaaa taaaacgaaa ggctcagtcg
 +
    1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
 +
    1981 actggctcac cttcgggtgg gcctttctgc gtttatatag tAGAGACCAG GTGC
 +
//`
 +
       
 +
sequences.seq_ACC_outro_gblock = {};
 +
sequences.seq_ACC_outro_gblock.varName = "seq_ACC_outro_gblock";
 +
sequences.seq_ACC_outro_gblock.name = "ACC outro gblock";
 +
sequences.seq_ACC_outro_gblock.seqType = "both";
 +
sequences.seq_ACC_outro_gblock.comp = true;
 +
sequences.seq_ACC_outro_gblock.data = `LOCUS      ACC_outro_gblock        2216 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    2179..2199
 +
                    /label="Suffix"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    388..417
 +
                    /label="Spacer"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    misc_feature    18..52
 +
                    /label="Promoter"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    2050..2178
 +
                    /label="Terminator"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    418..2049
 +
                    /label="ACCbeta"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    77..90
 +
                    /label="RBS"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    94..387
 +
                    /label="ZFA"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding    53..76
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 CCAGCCGGTC TCATAGTttg acggctagct cagtcctagg tatagtgcta gcTGGAATTG
 +
      61 TGAGCGGATA ACAATTGAAA GAGGTGACAT ATGACATCTG CAGCGGCCCC GGGGGAACGG
 +
      121 CCATTCCAGT GTCGGATTTG CATGAGAAAC TTTAGCGATT CACCGACCCT GCGTCGTCAC
 +
      181 ACCCGCACGC ATACTGGCGA AAAGCCTTTC CAGTGTCGCA TTTGCATGCG CAATTTTTCC
 +
      241 GTTCGTCATA ATTTAACGCG CCACCTTCGC ACGCACACCG GTGAGAAACC GTTCCAATGC
 +
      301 CGTATCTGTA TGCGTAACTT TTCGGACCGC ACTAGTCTGG CGCGACATTT GAAAACCCAT
 +
      361 TATCCCTATG ATGTGCCGGA TTACGCTggt ggaagtggag gtggaagtgg aggtagtATG
 +
      421 ACGATCAGTT CCCCGCTGAT CGATGTTGCT AACCTGCCGG ATATTAACAC CACGGCGGGA
 +
      481 AAAATAGCTG ATCTCAAAGC GCGCCGCGCG GAAGCCCATT TCCCTATGGG CGAAAAGGCG
 +
      541 GTTGAGAAAG TGCACGCCGC AGGTCGTCTG ACAGCACGGG AACGTCTGGA TTATCTCTTA
 +
      601 GATGAAGGCT CTTTCATCGA AACCGATCAA CTTGCCCGCC ACCGGACGAC GGCGTTTGGC
 +
      661 CTGGGCGCCA AACGTCCGGC AACCGATGGG ATCGTGACCG GGTGGGGTAC CATTGATGGC
 +
      721 CGCGAAGTCT GTATTTTCAG CCAGGATGGA ACTGTTTTTG GCGGCGCGCT GGGCGAGGTT
 +
      781 TACGGGGAAA AAATGATAAA AATTATGGAA TTAGCGATCG ATACGGGGCG TCCGCTGATT
 +
      841 GGCCTGTACG AGGGAGCGGG CGCCCGAATC CAGGATGGTG CAGTCTCGCT AGATTTCATT
 +
      901 AGTCAAACAT TTTACCAGAA TATCCAGGCC AGCGGAGTAA TTCCCCAGAT TAGTGTGATC
 +
      961 ATGGGCGCGT GCGCCGGTGG GAATGCTTAT GGCCCAGCGT TGACAGACTT CGTGGTGATG
 +
    1021 GTCGATAAGA CGTCTAAAAT GTTTGTGACG GGTCCAGATG TGATTAAGAC CGTGACTGGC
 +
    1081 GAGGAAATTA CCCAGGAGGA GCTTGGTGGT GCAACGACAC ACATGGTAAC TGCGGGAAAC
 +
    1141 TCCCATTATA CTGCGGCTAC CGACGAGGAA GCCTTGGATT GGGTTCAAGA TCTGGTTAGC
 +
    1201 TTTCTGCCTT CCAACAACCG GTCTTACACT CCCCTGGAAG ATTTTGACGA AGAAGAAGGT
 +
    1261 GGGGTTGAAG AAAATATCAC CGCAGACGAC CTTAAACTCG ACGAAATCAT TCCGGATTCA
 +
    1321 GCTACCGTAC CATACGACGT TCGTGACGTC ATCGAATGTC TTACCGATGA CGGTGAGTAT
 +
    1381 TTGGAGATTC AGGCGGACCG CGCCGAAAAC GTCGTGATTG CGTTTGGGCG CATTGAAGGT
 +
    1441 CAAAGCGTTG GCTTTGTGGC AAATCAGCCG ACTCAGTTCG CGGGTTGTCT GGATATTGAT
 +
    1501 TCATCGGAAA AAGCAGCACG TTTCGTGCGT ACGTGCGATG CGTTTAACAT CCCGATTGTA
 +
    1561 ATGCTGGTCG ACGTGCCGGG ATTTCTGCCG GGAGCCGGTC AAGAATATGG TGGTATTCTG
 +
    1621 AGACGCGGCG CTAAATTATT ATATGCATAT GGCGAAGCAA CAGTACCGAA AATCACCGTT
 +
    1681 ACCATGCGTA AGGCATACGG GGGTGCGTAC TGCGTCATGG GCTCAAAAGG CTTGGGCAGT
 +
    1741 GACATCAATC TAGCGTGGCC GACCGCTCAG ATTGCCGTGA TGGGTGCCGC GGGCGCAGTC
 +
    1801 GGCTTCATAT ATCGCAAAGA ACTGATGGCG GCCGATGCCA AAGGTCTGGA TACGGTGGCT
 +
    1861 CTGGCTAAAA GCTTTGAGCG CGAGTATGAA GACCACATGC TGAATCCTTA TCATGCTGCC
 +
    1921 GAGCGTGGGC TCATAGACGC GGTAATCTTA CCCTCGGAAA CCAGGGGCCA GATTAGCCGA
 +
    1981 AATCTGCGTC TTCTGAAGCA TAAAAATGTG ACACGCCCAG CCAGAAAACA TGGTAACATG
 +
    2041 CCTTTGTAAc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
 +
    2101 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
 +
    2161 ggcctttctg cgtttatata ctagtagcgg ccgctgcagT GCCAGAGACC CCATTA
 +
//`
 +
       
 +
sequences.seq_ACS_intro_gblock = {};
 +
sequences.seq_ACS_intro_gblock.varName = "seq_ACS_intro_gblock";
 +
sequences.seq_ACS_intro_gblock.name = "ACS intro gblock";
 +
sequences.seq_ACS_intro_gblock.seqType = "linear";
 +
sequences.seq_ACS_intro_gblock.comp = true;
 +
sequences.seq_ACS_intro_gblock.data = `LOCUS      ACS_intro_gblock        458 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    18..39
 +
                    /label="Prefix"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    40..74
 +
                    /label="promoter"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    122..415
 +
                    /label="ZFB"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    misc_binding    75..98
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    99..118
 +
                    /label="RBS"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    416..445
 +
                    /label="Spacer"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
ORIGIN
 +
        1 GTTTCAGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
 +
      61 ggtacagtgc tagcTGGAAT TGTGAGCGGA TAACAATTTC GACTCGTTCG ATTAAAGAAT
 +
      121 GACCAGTGCG GCTGCCCCTG GTGAACGCCC GTTTCAGTGT CGGATTTGCA TGCGAAATTT
 +
      181 CAGCAAAAAA GATCATCTCC ACCGTCATAC TCGCACACAC ACTGGGGAAA AACCGTTCCA
 +
      241 GTGTCGCATT TGCATGCGTA ACTTTTCCTT GTCACAAACC CTGAAACGTC ACCTTCGCAC
 +
      301 GCATACGGGC GAGAAGCCCT TCCAGTGTAG AATCTGCATG CGCAACTTTT CGCGTTTAGA
 +
      361 TATGCTGGCG CGTCATCTGA AAACCCATTA TCCATACGAT GTGCCGGACT ATGCAggtgg
 +
      421 aagtggaggt ggaagtggag gtagtAGAGA CCGGACTA
 +
//`
 +
       
 +
sequences.seq_ACS_outro_gblock = {};
 +
sequences.seq_ACS_outro_gblock.varName = "seq_ACS_outro_gblock";
 +
sequences.seq_ACS_outro_gblock.name = "ACS outro gblock";
 +
sequences.seq_ACS_outro_gblock.seqType = "linear";
 +
sequences.seq_ACS_outro_gblock.comp = true;
 +
sequences.seq_ACS_outro_gblock.data = `LOCUS      ACS_outro_gblock        2143 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    2106..2126
 +
                    /label="Suffix"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    18..1973
 +
                    /label="ACS"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    misc_feature    1977..2105
 +
                    /label="Terminator"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 GTTTCAGGTC TCATAGTATG TCTCAGATTC ACAAACATAC TATCCCTGCA AACATTGCTG
 +
      61 ACCGTTGCCT GATTAATCCT CAACAGTATG AAGCCATGTA CCAGCAGTCG ATTAATGTGC
 +
      121 CGGATACATT TTGGGGCGAA CAGGGGAAGA TTCTGGACTG GATCAAACCG TACCAAAAAG
 +
      181 TGAAAAATAC CAGTTTCGCA CCGGGTAATG TTAGCATCAA ATGGTACGAA GACGGCACTC
 +
      241 TTAACCTTGC GGCGAATTGT CTGGATCGTC ATCTACAAGA AAACGGCGAT CGTACAGCGA
 +
      301 TTATATGGGA GGGTGATGAT GCTAGCCAGT CGAAGCATAT CAGCTATAAA GAACTTCATC
 +
      361 GCGACGTATG CCGCTTCGCA AACACGTTGT TAGAATTAGG CATCAAGAAA GGTGATGTGG
 +
      421 TCGCCATTTA TATGCCAATG GTTCCGGAAG CGGCCGTGGC TATGCTCGCA TGCGCCCGTA
 +
      481 TTGGAGCGGT CCATTCGGTT ATTTTTGGGG GGTTTTCCCC GGAGGCAGTC GCCGGCCGCA
 +
      541 TTATTGATTC CAATAGCCGT CTCGTCATCA CCTCTGATGA AGGAGTTCGG GCGGGCCGCA
 +
      601 GCATTCCTCT GAAAAAGAAT GTAGACGATG CTCTGAAAAA CCCTAACGTC ACGAGTGTCG
 +
      661 AGCACGTTGT TGTGTTAAAA CGCACCGGTG GTAAAATTGA CTGGCAGGAA GGGCGTGACT
 +
      721 TGTGGTGGCA TGACCTTGTA GAACAAGCGT CCGATCAGCA TCAGGCTGAA GAGATGAACG
 +
      781 CAGAGGACCC GCTGTTCATC TTATATACCA GCGGATCTAC AGGTAAACCC AAAGGGGTGC
 +
      841 TGCATACTAC GGGCGGTTAT TTGGTGTACG CCGCCCTGAC TTTTAAATAC GTCTTTGATT
 +
      901 ACCACCCCGG TGACATCTAT TGGTGTACCG CTGATGTCGG TTGGGTTACC GGACATAGCT
 +
      961 ATCTGCTGTA TGGCCCCTTG GCATGTGGGG CCACAACGTT GATGTTTGAA GGAGTGCCGA
 +
    1021 ATTGGCCGAC CCCAGCCCGC ATGGCGCAGG TAGTTGATAA GCATCAGGTG AACATCCTGT
 +
    1081 ACACGGCTCC GACGGCCATT CGAGCTCTGA TGGCGGAAGG CGATAAAGCG ATAGAAGGCA
 +
    1141 CGGATCGCTC TTCTCTCCGC ATCCTGGGTT CAGTTGGCGA GCCAATCAAC CCAGAGGCGT
 +
    1201 GGGAGTGGTA CTGGAAAAAA ATTGGTAATG AAAAATGCCC GGTCGTGGAC ACCTGGTGGC
 +
    1261 AGACCGAAAC GGGAGGCTTT ATGATTACCC CGCTGCCAGG CGCGACGGAG CTAAAGGCAG
 +
    1321 GGAGCGCAAC AAGGCCTTTT TTCGGTGTGC AACCGGCCTT AGTGGACAAT GAGGGTAATC
 +
    1381 CGCTGGAAGG GGCGACCGAG GGATCACTGG TGATTACTGA CAGTTGGCCG GGCCAGGCAC
 +
    1441 GAACCCTGTT CGGTGATCAC GAAAGATTCG AACAAACGTA TTTCTCCACA TTTAAAAACA
 +
    1501 TGTATTTTTC AGGTGACGGC GCGCGTAGAG ATGAAGATGG TTATTATTGG ATCACCGGCC
 +
    1561 GGGTCGATGA TGTGCTCAAC GTTAGCGGTC ACCGTCTTGG CACGGCGGAA ATCGAATCGG
 +
    1621 CTCTGGTGGC CCACCCTAAG ATCGCGGAGG CAGCCGTGGT AGGCATTCCG CATAACATAA
 +
    1681 AAGGCCAGGC CATTTACGCG TATGTGACTT TGAATCACGG CGAAGAACCA TCGCCGGAAT
 +
    1741 TATACGCGGA AGTACGCAAC TGGGTTCGGA AAGAAATCGG TCCACTGGCA ACCCCGGATG
 +
    1801 TACTGCACTG GACCGATTCA CTGCCCAAAA CGCGCAGTGG GAAGATCATG CGTCGCATAC
 +
    1861 TGCGTAAAAT TGCGGCCGGT GATACTTCAA ACCTCGGCGA TACCTCCACC CTTGCAGATC
 +
    1921 CCGGCGTAGT TGAAAAACTG CTGGAAGAGA AACAAGCCAT TGCGATGCCG AGTTGAccag
 +
    1981 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
 +
    2041 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
 +
    2101 ttatatacta gtagcggccg ctgcagTGCC AGAGACCGGA CTA
 +
//`
 +
       
 +
sequences.seq_BsaI_FWD_primer = {};
 +
sequences.seq_BsaI_FWD_primer.varName = "seq_BsaI_FWD_primer";
 +
sequences.seq_BsaI_FWD_primer.name = "BsaI FWD primer";
 +
sequences.seq_BsaI_FWD_primer.seqType = "linear";
 +
sequences.seq_BsaI_FWD_primer.comp = false;
 +
sequences.seq_BsaI_FWD_primer.data = `LOCUS      BsaI_FWD_primer          35 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 CTCTCAGGTC TCATGCCagt ccggcaaaaa agggc
 +
//`
 +
       
 +
sequences.seq_BsaI_REV_primer = {};
 +
sequences.seq_BsaI_REV_primer.varName = "seq_BsaI_REV_primer";
 +
sequences.seq_BsaI_REV_primer.name = "BsaI REV primer";
 +
sequences.seq_BsaI_REV_primer.seqType = "linear";
 +
sequences.seq_BsaI_REV_primer.comp = false;
 +
sequences.seq_BsaI_REV_primer.data = `LOCUS      BsaI_REV_primer          40 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ATCTTAGGTC TCTCTCCcag aaatcatcct tagcgaaagc
 +
//`
 +
       
 +
sequences.seq_CoA_4x_Assembly = {};
 +
sequences.seq_CoA_4x_Assembly.varName = "seq_CoA_4x_Assembly";
 +
sequences.seq_CoA_4x_Assembly.name = "CoA 4x Assembly";
 +
sequences.seq_CoA_4x_Assembly.seqType = "both";
 +
sequences.seq_CoA_4x_Assembly.comp = true;
 +
sequences.seq_CoA_4x_Assembly.data = `LOCUS      CoA_4x_Assembly        2492 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2480..2488
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2469..2477
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Zinc Finger Site    2458..2466
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    2447..2455
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2436..2444
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2414..2422
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2403..2411
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    2425..2433
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    HIV-RT Binding Site join(2489..2492,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
 +
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AA
 +
//`
 +
       
 +
sequences.seq_CoA_4x_Comp_Assembly = {};
 +
sequences.seq_CoA_4x_Comp_Assembly.varName = "seq_CoA_4x_Comp_Assembly";
 +
sequences.seq_CoA_4x_Comp_Assembly.name = "CoA 4x Comp Assembly";
 +
sequences.seq_CoA_4x_Comp_Assembly.seqType = "both";
 +
sequences.seq_CoA_4x_Comp_Assembly.comp = true;
 +
sequences.seq_CoA_4x_Comp_Assembly.data = `LOCUS      CoA_4x_Comp_Assembly    2492 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2425..2433)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2480..2488)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2469..2477)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    complement(2447..2455)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Zinc Finger Site    complement(2414..2422)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2403..2411)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2436..2444)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2392..2400)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    HIV-RT Binding Site join(2489..2492,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2458..2466)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
 +
    2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
 +
    2461 AGCCGCATGG CATCGACATG CCGTCCTCAA AA
 +
//`
 +
       
 +
sequences.seq_CoA_4x_Full_Part = {};
 +
sequences.seq_CoA_4x_Full_Part.varName = "seq_CoA_4x_Full_Part";
 +
sequences.seq_CoA_4x_Full_Part.name = "CoA 4x Full Part";
 +
sequences.seq_CoA_4x_Full_Part.seqType = "both";
 +
sequences.seq_CoA_4x_Full_Part.comp = true;
 +
sequences.seq_CoA_4x_Full_Part.data = `LOCUS      CoA_4x_Full_Part        2835 bp ds-DNA    circular    14-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2779..2787)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2425..2433
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2447..2455
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    HIV-RT Binding Site 2489..2598
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    2436..2444
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    2414..2422
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2746..2754)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Scar                2668..2675
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2458..2466
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site join(2832..2835,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2735..2743)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2768..2776)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2790..2798)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2801..2809)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Promoter            2676..2710
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2812..2820)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2403..2411
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2480..2488
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    gBlock              2545..2669
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    2469..2477
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2757..2765)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          2599..2667
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding        2712..2734
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2823..2831)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
 +
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
 +
    2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
 +
    2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
 +
    2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
 +
    2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTCCCAGC CGCATGGCAT CGACATCCCA
 +
    2761 GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
 +
    2821 ATGCCGTCCT CAAAA
 +
//`
 +
       
 +
sequences.seq_CoA_4x_r_oligo_Complement_Duplex = {};
 +
sequences.seq_CoA_4x_r_oligo_Complement_Duplex.varName = "seq_CoA_4x_r_oligo_Complement_Duplex";
 +
sequences.seq_CoA_4x_r_oligo_Complement_Duplex.name = "CoA 4x r_oligo Complement Duplex";
 +
sequences.seq_CoA_4x_r_oligo_Complement_Duplex.seqType = "linear";
 +
sequences.seq_CoA_4x_r_oligo_Complement_Duplex.comp = true;
 +
sequences.seq_CoA_4x_r_oligo_Complement_Duplex.data = `LOCUS      CoA_4x_r_oligo_Complemen 155 bp ds-DNA    linear      25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site complement(42..50)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(53..61)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(64..72)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(75..83)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(86..94)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(97..105)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(108..116)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(119..127)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(130..138)
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          complement(42..138)
 +
                    /label="Temp2"
 +
                    /note="sequence: GAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          42..138
 +
                    /label="Temp"
 +
                    /note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTC"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
 +
      61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
 +
      121 CATCGACATG CCGTCCTCAA AACGAGACCA ACCTG
 +
//`
 +
       
 +
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1 = {};
 +
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.varName = "seq_CoA_4x_r_oligo_Duplex_126145_REV_1";
 +
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.name = "CoA 4x r_oligo Duplex 126-145 REV 1";
 +
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.seqType = "linear";
 +
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.comp = false;
 +
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.data = `LOCUS      CoA_4x_r_oligo_Duplex_126 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCGTTTTCCC AGCCGCATGG
 +
//`
 +
       
 +
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1 = {};
 +
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.varName = "seq_CoA_4x_r_oligo_Duplex_6988_FWD_1";
 +
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.name = "CoA 4x r_oligo Duplex 69-88 FWD 1";
 +
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.seqType = "linear";
 +
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.comp = false;
 +
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.data = `LOCUS      CoA_4x_r_oligo_Duplex_69- 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TGGGATGTCG ATGCCATGCG
 +
//`
 +
       
 +
sequences.seq_CoA_4x_r_oligo_Duplex = {};
 +
sequences.seq_CoA_4x_r_oligo_Duplex.varName = "seq_CoA_4x_r_oligo_Duplex";
 +
sequences.seq_CoA_4x_r_oligo_Duplex.name = "CoA 4x r_oligo Duplex";
 +
sequences.seq_CoA_4x_r_oligo_Duplex.seqType = "linear";
 +
sequences.seq_CoA_4x_r_oligo_Duplex.comp = true;
 +
sequences.seq_CoA_4x_r_oligo_Duplex.data = `LOCUS      CoA_4x_r_oligo_Duplex    155 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site 42..50
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site 53..61
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 64..72
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 75..83
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 86..94
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 97..105
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 108..116
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 119..127
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 130..138
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          complement(126..145)
 +
                    /label="CoA 4x r_oligo Duplex 126-145 REV 1"
 +
                    /note="sequence: TCGTTTTCCCAGCCGCATGG"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          18..138
 +
                    /label="Temp Fwd"
 +
                    /note="sequence: TGGAATTGTGAGCGGATAACAATTGAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          complement(20..140)
 +
                    /label="Temp Rev"
 +
                    /note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTCAATTGTTATCCGCTCACAATTCCA"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    primer          69..88
 +
                    /label="CoA 4x r_oligo Duplex 69-88 FWD 1"
 +
                    /note="sequence: TGGGATGTCGATGCCATGCG"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
 +
      61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
 +
      121 CGATGCCATG CGGCTGGGAA AACGAGACCA ACCTG
 +
//`
 +
       
 +
sequences.seq_CoA_6x_Assembly = {};
 +
sequences.seq_CoA_6x_Assembly.varName = "seq_CoA_6x_Assembly";
 +
sequences.seq_CoA_6x_Assembly.name = "CoA 6x Assembly";
 +
sequences.seq_CoA_6x_Assembly.seqType = "both";
 +
sequences.seq_CoA_6x_Assembly.comp = true;
 +
sequences.seq_CoA_6x_Assembly.data = `LOCUS      CoA_6x_Assembly        2536 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2502..2510
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    2491..2499
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2480..2488
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2524..2532
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2469..2477
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2458..2466
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2513..2521
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2447..2455
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2436..2444
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2425..2433
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2414..2422
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2403..2411
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    HIV-RT Binding Site join(2533..2536,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
 +
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
 +
    2521 CATGCGGCTG GGAAAA
 +
//`
 +
       
 +
sequences.seq_CoA_6x_Comp_Assembly = {};
 +
sequences.seq_CoA_6x_Comp_Assembly.varName = "seq_CoA_6x_Comp_Assembly";
 +
sequences.seq_CoA_6x_Comp_Assembly.name = "CoA 6x Comp Assembly";
 +
sequences.seq_CoA_6x_Comp_Assembly.seqType = "both";
 +
sequences.seq_CoA_6x_Comp_Assembly.comp = true;
 +
sequences.seq_CoA_6x_Comp_Assembly.data = `LOCUS      CoA_6x_Comp_Assembly    2536 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    complement(2491..2499)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2502..2510)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2480..2488)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2458..2466)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2447..2455)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    complement(2436..2444)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2425..2433)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2414..2422)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2403..2411)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2392..2400)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2513..2521)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2524..2532)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    HIV-RT Binding Site join(2533..2536,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    complement(2469..2477)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
 +
    2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
 +
    2461 AGCCGCATGG CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA
 +
    2521 CATGCCGTCC TCAAAA
 +
//`
 +
       
 +
sequences.seq_CoA_6x_Full_Part = {};
 +
sequences.seq_CoA_6x_Full_Part.varName = "seq_CoA_6x_Full_Part";
 +
sequences.seq_CoA_6x_Full_Part.name = "CoA 6x Full Part";
 +
sequences.seq_CoA_6x_Full_Part.seqType = "both";
 +
sequences.seq_CoA_6x_Full_Part.comp = true;
 +
sequences.seq_CoA_6x_Full_Part.data = `LOCUS      CoA_6x_Full_Part        2923 bp ds-DNA    circular    14-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Zinc Finger Site    2425..2433
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_binding        2756..2778
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2790..2798)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2469..2477
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2900..2908)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2480..2488
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2447..2455
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2867..2875)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site 2533..2642
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2524..2532
 +
                        /label="ZFb Site"
 +
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 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    complement(2823..2831)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2458..2466
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2812..2820)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2436..2444
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
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    Zinc Finger Site    complement(2878..2886)
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                        /label="ZFa Site"
 +
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 +
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    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
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 +
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 +
    Zinc Finger Site    2491..2499
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2856..2864)
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 +
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 +
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    Zinc Finger Site    2502..2510
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                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
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    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
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 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Terminator          2643..2711
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2834..2842)
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2513..2521
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    complement(2779..2787)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Zinc Finger Site    2403..2411
 +
                        /label="ZFa Site"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Promoter            2720..2754
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2845..2853)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Scar                2712..2719
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2414..2422
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    gBlock              2589..2713
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2889..2897)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2801..2809)
 +
                        /label="ZFb Site"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site join(2920..2923,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2911..2919)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
 +
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
 +
    2521 CATGCGGCTG GGAAAAAAAA CGTGGCGCCC GAACAGGGAC GGATAGCTCA GTCGGTAGAG
 +
    2581 CATCAGACTT TTAATCTGAG GGTCCAGGGT TCAAGTCCCT GTTCGGGCGC CACGTTTTTT
 +
    2641 TTattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact tcccttgggg
 +
    2701 ttatcattgg gtactagagT TGACGGCTAG CTCAGTCCTA GGTACAGTGC TAGCTGGAAT
 +
    2761 TGTGAGCGGA TAACAATTCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
 +
    2821 ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT CGACATCCCA GCCGCATGGC
 +
    2881 ATCGACATCC CAGCCGCATG GCATCGACAT GCCGTCCTCA AAA
 +
//`
 +
       
 +
sequences.seq_CoA_6x_r_oligo_Complement_Duplex = {};
 +
sequences.seq_CoA_6x_r_oligo_Complement_Duplex.varName = "seq_CoA_6x_r_oligo_Complement_Duplex";
 +
sequences.seq_CoA_6x_r_oligo_Complement_Duplex.name = "CoA 6x r_oligo Complement Duplex";
 +
sequences.seq_CoA_6x_r_oligo_Complement_Duplex.seqType = "linear";
 +
sequences.seq_CoA_6x_r_oligo_Complement_Duplex.comp = true;
 +
sequences.seq_CoA_6x_r_oligo_Complement_Duplex.data = `LOCUS      CoA_6x_r_oligo_Complemen 199 bp ds-DNA    linear      25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site complement(42..50)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(53..61)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(64..72)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(75..83)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(86..94)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(97..105)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(108..116)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(119..127)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(130..138)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(141..149)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(152..160)
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site complement(163..171)
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site complement(174..182)
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
 +
      61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
 +
      121 CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA CATGCCGTCC
 +
      181 TCAAAACGAG ACCAACCTG
 +
//`
 +
       
 +
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1 = {};
 +
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.varName = "seq_CoA_6x_r_oligo_Duplex_113132_FWD_1";
 +
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.name = "CoA 6x r_oligo Duplex 113-132 FWD 1";
 +
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.seqType = "linear";
 +
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.comp = false;
 +
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.data = `LOCUS      CoA_6x_r_oligo_Duplex_113 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TGGGATGTCG ATGCCATGCG
 +
//`
 +
       
 +
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1 = {};
 +
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.varName = "seq_CoA_6x_r_oligo_Duplex_170189_REV_1";
 +
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.name = "CoA 6x r_oligo Duplex 170-189 REV 1";
 +
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.seqType = "linear";
 +
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.comp = false;
 +
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.data = `LOCUS      CoA_6x_r_oligo_Duplex_170 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCGTTTTCCC AGCCGCATGG
 +
//`
 +
       
 +
sequences.seq_CoA_6x_r_oligo_Duplex = {};
 +
sequences.seq_CoA_6x_r_oligo_Duplex.varName = "seq_CoA_6x_r_oligo_Duplex";
 +
sequences.seq_CoA_6x_r_oligo_Duplex.name = "CoA 6x r_oligo Duplex";
 +
sequences.seq_CoA_6x_r_oligo_Duplex.seqType = "linear";
 +
sequences.seq_CoA_6x_r_oligo_Duplex.comp = true;
 +
sequences.seq_CoA_6x_r_oligo_Duplex.data = `LOCUS      CoA_6x_r_oligo_Duplex    199 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site 42..50
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site 53..61
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 64..72
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 75..83
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 86..94
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 97..105
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 108..116
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 119..127
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 130..138
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 141..149
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 152..160
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site 163..171
 +
                    /label="ZFa Site"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site 174..182
 +
                    /label="ZFb Site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          113..132
 +
                    /label="CoA 6x r_oligo Duplex 113-132 FWD 1"
 +
                    /note="sequence: TGGGATGTCGATGCCATGCG"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          complement(170..189)
 +
                    /label="CoA 6x r_oligo Duplex 170-189 REV 1"
 +
                    /note="sequence: TCGTTTTCCCAGCCGCATGG"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
 +
      61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
 +
      121 CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG
 +
      181 GGAAAACGAG ACCAACCTG
 +
//`
 +
       
 +
sequences.seq_gblock_1 = {};
 +
sequences.seq_gblock_1.varName = "seq_gblock_1";
 +
sequences.seq_gblock_1.name = "g-block 1";
 +
sequences.seq_gblock_1.seqType = "linear";
 +
sequences.seq_gblock_1.comp = true;
 +
sequences.seq_gblock_1.data = `LOCUS      g-block_1                300 bp ds-DNA    linear      07-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    164..213
 +
                    /label="sTRSV HHRz"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    Biobricking    68..89
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_binding    126..148
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    90..124
 +
                    /label="BBa_J23109'"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    RBS            222..233
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
ORIGIN
 +
        1 TGTTGATTGG TGGCACATAA GTAATACCAT GGTCCCTGAA ATTCGGCTCA ACGGTCCGTC
 +
      61 TCATCTGGAA TTCGCGGCCG CTTCTAGAGt ttacagctag ctcagtccta gggactgtgc
 +
      121 tagcTGGAAT TGTGAGCGGA TAACAATTct atggactatg tttCTGTCAC CGGATGTGCT
 +
      181 TTCCGGTCTG ATGAGTCCGT GAGGACGAAA CAGtaccagt gaaagaggag aaaGGATTGA
 +
      241 GACGGTAACA GTTACTTCGA GCGTAATGTC TCAAATGGCG TAGAACGGCA ATGACTGTTc
 +
//`
 +
       
 +
sequences.seq_gblock_3 = {};
 +
sequences.seq_gblock_3.varName = "seq_gblock_3";
 +
sequences.seq_gblock_3.name = "g-block 3";
 +
sequences.seq_gblock_3.seqType = "linear";
 +
sequences.seq_gblock_3.comp = true;
 +
sequences.seq_gblock_3.data = `LOCUS      g-block_3                300 bp ds-DNA    linear      07-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Biobricking    199..219
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    terminator      112..198
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          complement(177..198)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: CTCACGCGTCTCAGCTACTGCAGCGGCCGCTACTAGTAatttgtcctactcaggagagcg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(177..198)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: CTCACGCGTCTCAGCTAatttgtcctactcaggagagcg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(170..198)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: atttgtcctactcaggagagcgttcaccg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
ORIGIN
 +
        1 GGGAGGTGGA ATAGTTTCGA ACGATGGTTA TTAATCGTAA TAACGGAACG CTGTCTGGAG
 +
      61 GATGATTCTC ACGTCTCAAG GGccttcagg catgcaagcc tctggaggca tcaaataaaa
 +
      121 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
 +
      181 ctcctgagta ggacaaatTA CTAGTAGCGG CCGCTGCAGA GTCAGAGACG GTAACATCGA
 +
      241 AGGGGAACTA TTTCGTGGGG CGAGCCCACA CCGTCTCTTC TGCGGAAGAC TTAACACGTT
 +
//`
 +
       
 +
sequences.seq_gBlock_A = {};
 +
sequences.seq_gBlock_A.varName = "seq_gBlock_A";
 +
sequences.seq_gBlock_A.name = "gBlock A";
 +
sequences.seq_gBlock_A.seqType = "linear";
 +
sequences.seq_gBlock_A.comp = true;
 +
sequences.seq_gBlock_A.data = `LOCUS      gBlock_A                995 bp ds-DNA    linear      02-SEP-2020
 
DEFINITION  .
 
DEFINITION  .
 
FEATURES            Location/Qualifiers
 
FEATURES            Location/Qualifiers
Line 96: Line 2,952:
 
       961 AGTCGGTAGA GCATCAGACT TTCGAGACGC TTGTG
 
       961 AGTCGGTAGA GCATCAGACT TTCGAGACGC TTGTG
 
//`
 
//`
−
 
+
       
−
sequences.roligoFlipper = {};
+
sequences.seq_gBlock_B = {};
−
sequences.roligoFlipper.name = "r_oligo Flipper"
+
sequences.seq_gBlock_B.varName = "seq_gBlock_B";
−
sequences.roligoFlipper.seqType = "both";
+
sequences.seq_gBlock_B.name = "gBlock B";
−
sequences.roligoFlipper.data = `LOCUS      r_oligo_Flipper        3220 bp ds-DNA    circular    02-SEP-2020
+
sequences.seq_gBlock_B.seqType = "linear";
 +
sequences.seq_gBlock_B.comp = true;
 +
sequences.seq_gBlock_B.data = `LOCUS      gBlock_B                175 bp ds-DNA    linear      20-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Biobricking    138..158
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    15..158
 +
                    /label="gBlock B"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    misc            72..111
 +
                    /label="identical to E. coli MG1655 between tonB and yciA"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    misc_feature    69..137
 +
                    /label="B0054"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    stem_loop      79..110
 +
                    /label="stem loop"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 GATCGTCGTC TCCCTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC GGGCGCCACG
 +
      61 TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc
 +
      121 ttggggttat cattgggtac tagtagcggc cgctgcagag tcCGAGACGT TCCAT
 +
//`
 +
       
 +
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear = {};
 +
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.varName = "seq_GFP_final_synthesized_for_pSB1T3_linear";
 +
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.name = "GFP final synthesized for pSB1T3 linear";
 +
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.seqType = "linear";
 +
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.comp = true;
 +
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.data = `LOCUS      GFP_final_synthesized_f 1240 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature        91..405
 +
                          /label="Zif268 + linker"
 +
                          /ApEinfo_revcolor=#84b0dc
 +
                          /ApEinfo_fwdcolor=#84b0dc
 +
    Recognition Sequence 7..12
 +
                          /label="BsaI 5' site"
 +
                          /ApEinfo_revcolor=#f58a5e
 +
                          /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature        1..6
 +
                          /label="rando bases"
 +
                          /ApEinfo_revcolor=#b7e6d7
 +
                          /ApEinfo_fwdcolor=#b7e6d7
 +
    misc_feature        1235..1240
 +
                          /label="rando bases"
 +
                          /ApEinfo_revcolor=#d6b295
 +
                          /ApEinfo_fwdcolor=#d6b295
 +
    misc_feature        18..52
 +
                          /label="new promoter"
 +
                          /ApEinfo_revcolor=#faac61
 +
                          /ApEinfo_fwdcolor=#faac61
 +
    misc_feature        406..1122
 +
                          /label="GFP"
 +
                          /ApEinfo_revcolor=#b1ff67
 +
                          /ApEinfo_fwdcolor=#b1ff67
 +
    Recognition Sequence 1229..1234
 +
                          /label="BsaI 3' site"
 +
                          /ApEinfo_revcolor=#f58a5e
 +
                          /ApEinfo_fwdcolor=#f58a5e
 +
    Scar                1170..1177
 +
                          /label="BioBrick Scar"
 +
                          /ApEinfo_revcolor=#9eafd2
 +
                          /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature        1129..1223
 +
                          /label="BBa_B0014 Double Terminator"
 +
                          /ApEinfo_revcolor=#9eafd2
 +
                          /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature        77..90
 +
                          /label="RBS - J61111"
 +
                          /ApEinfo_revcolor=#ff9ccd
 +
                          /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_binding        54..76
 +
                          /label="LacO"
 +
                          /ApEinfo_revcolor=#84b0dc
 +
                          /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 GATACGGGTC TCACGTTttt acggctagct cagtcctagg tatagtgcta gcTGGAATTG
 +
      61 TGAGCGGATA ACAATTGAAA GAGGCGATAC ATGCACCATC ACCATCATCA CCCGGGTGAG
 +
      121 AAGCCATATG CCTGCCCCGT GGAAAGCTGC GATCGTCGTT TCAGTCGCTC TGATGAACTG
 +
      181 ACACGCCACA TCAGAATACA TACCGGGCAG AAACCGTTTC AATGTCGCAT TTGTATGCGC
 +
      241 AACTTTTCGC GCAGCGACCA TTTAACTACG CATATCCGTA CCCACACTGG TGAAAAACCT
 +
      301 TTCGCGTGTG ATATTTGCGG ACGAAAATTT GCACGGTCAG ACGAGCGTAA ACGTCATACC
 +
      361 AAAATTCATA CGGGCGGCGG CAGCGGCGGC GGTAGTGGTG GGTCCcgtaa aggagaagaa
 +
      421 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
 +
      481 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
 +
      541 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
 +
      601 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
 +
      661 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
 +
      721 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
 +
      781 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
 +
      841 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
 +
      901 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
 +
      961 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
 +
    1021 ctttcgaaag atcccaacga aaagAGGgac cacatggtcc ttcttgagtt tgtaacagct
 +
    1081 gctgggatta cacatggcat ggatgaacta tacaaataat aatggctgtc acactggctc
 +
    1141 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
 +
    1201 ttaatccggc ttttttatta tttTGCCaGA GACCAGGCTT
 +
//`
 +
       
 +
sequences.seq_GFP_Fusion_in_TetR = {};
 +
sequences.seq_GFP_Fusion_in_TetR.varName = "seq_GFP_Fusion_in_TetR";
 +
sequences.seq_GFP_Fusion_in_TetR.name = "GFP Fusion in TetR";
 +
sequences.seq_GFP_Fusion_in_TetR.seqType = "both";
 +
sequences.seq_GFP_Fusion_in_TetR.comp = true;
 +
sequences.seq_GFP_Fusion_in_TetR.data = `LOCUS      GFP_Fusion_in_TetR      3675 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    2539..2853
 +
                    /label="Zif268 + linker"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    2466..2500
 +
                    /label="new promoter"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    terminator      2394..2437
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2525..2538
 +
                    /label="RBS - J61111"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    primer_bind    complement(103..120)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    Biobricking    2440..2461
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    3672..3675
 +
                    /label="3' overhang"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Scar            3618..3625
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      22..79
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    3577..3671
 +
                    /label="BBa_B0014 Double Terminator"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    Biobricking    1..21
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    2854..3570
 +
                    /label="GFP"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    misc_feature    1..21
 +
                    /label="BioBrick suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind    2372..2390
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    rep_origin      complement(274..862)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    2462..2465
 +
                    /label="5' overhang"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind    complement(354..373)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    CDS            1128..2312
 +
                    /label="TetR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2440..2461
 +
                    /label="BioBrick prefix"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer          complement(2444..2461)
 +
                    /label="Copy of pSB1T3 REV"
 +
                    /note="sequence: AAGGTCGGTCTCCCTCTctctagaagcggccgcga"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          1..18
 +
                    /label="Copy of pSB1T3 FWD"
 +
                    /note="sequence: AAGGTCGGTCTCCTACTtactagtagcggccgctg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
ORIGIN
 +
        1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
 +
      61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
 +
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
 +
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
 +
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
 +
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
 +
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
 +
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
 +
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
 +
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
 +
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
 +
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
 +
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
 +
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
 +
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
 +
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
 +
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
 +
    1021 ttggtctgac agctcgagat tctcatgttt gacagcttat catcgataag ctttaatgcg
 +
    1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
 +
    1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
 +
    1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
 +
    1261 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
 +
    1321 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
 +
    1381 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
 +
    1441 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
 +
    1501 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
 +
    1561 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
 +
    1621 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
 +
    1681 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
 +
    1741 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
 +
    1801 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
 +
    1861 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
 +
    1921 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
 +
    1981 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
 +
    2041 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
 +
    2101 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
 +
    2161 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
 +
    2221 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
 +
    2281 gtcgcggtgc atggagccgg gccacctcga cctaactcga gtgccacctg acgtctaaga
 +
    2341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
 +
    2401 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
 +
    2461 gAGAGtttac ggctagctca gtcctaggta tagtgctagc TGGAATTGTG AGCGGATAAC
 +
    2521 AATTGAAAGA GGCGATACAT GCACCATCAC CATCATCACC CGGGTGAGAA GCCATATGCC
 +
    2581 TGCCCCGTGG AAAGCTGCGA TCGTCGTTTC AGTCGCTCTG ATGAACTGAC ACGCCACATC
 +
    2641 AGAATACATA CCGGGCAGAA ACCGTTTCAA TGTCGCATTT GTATGCGCAA CTTTTCGCGC
 +
    2701 AGCGACCATT TAACTACGCA TATCCGTACC CACACTGGTG AAAAACCTTT CGCGTGTGAT
 +
    2761 ATTTGCGGAC GAAAATTTGC ACGGTCAGAC GAGCGTAAAC GTCATACCAA AATTCATACG
 +
    2821 GGCGGCGGCA GCGGCGGCGG TAGTGGTGGG TCCcgtaaag gagaagaact tttcactgga
 +
    2881 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
 +
    2941 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
 +
    3001 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
 +
    3061 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
 +
    3121 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
 +
    3181 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
 +
    3241 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
 +
    3301 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
 +
    3361 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
 +
    3421 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
 +
    3481 cccaacgaaa agAGGgacca catggtcctt cttgagtttg taacagctgc tgggattaca
 +
    3541 catggcatgg atgaactata caaataataa tggctgtcac actggctcac cttcgggtgg
 +
    3601 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt
 +
    3661 ttttattatt tTACT
 +
//`
 +
       
 +
sequences.seq_GGE114 = {};
 +
sequences.seq_GGE114.varName = "seq_GGE114";
 +
sequences.seq_GGE114.name = "GGE114";
 +
sequences.seq_GGE114.seqType = "both";
 +
sequences.seq_GGE114.comp = true;
 +
sequences.seq_GGE114.data = `LOCUS      (Addgene_#120731_)_GGE1 5268 bp ds-DNA    circular    06-OCT-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_120731_229341"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    terminator      4070..4113
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2002..4115
 +
                    /label="pSB1A3 Region"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    protein_bind    4452..4485
 +
                    /label="loxP"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    source          1..5268
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind    3638..3657
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    promoter        complement(3876..3980)
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    CDS            722..1399
 +
                    /label="mRFP1"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    CDS            complement(3015..3875)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    rep_origin      complement(2256..2844)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    promoter        632..662
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind    complement(2085..2102)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    terminator      2004..2061
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    protein_bind    670..686
 +
                    /label="lac operator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    protein_bind    596..617
 +
                    /label="CAP binding site"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    terminator      1444..1515
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind    4048..4066
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind    complement(2336..2355)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature    3156..3156
 +
                    /label="Point Mutation"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      1531..1558
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    RBS            704..715
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    primer          2002..2019
 +
                    /label="Linear pSB1A3 BsmBI Fwd"
 +
                    /note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          complement(4088..4115)
 +
                    /label="BBa_Prefix & Promoter SDM Rev"
 +
                    /note="sequence: cagaaatcatccttagcgaaagctaagg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(4093..4115)
 +
                    /label="BsaI pSB1A3 Rev"
 +
                    /note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          4116..4135
 +
                    /label="BBa_Prefix & Promoter SDM Fwd"
 +
                    /note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          2002..2019
 +
                    /label="BsaI pSB1A3 Fwd"
 +
                    /note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          complement(4093..4115)
 +
                    /label="Linear pSB1A3 BsmBI Rev"
 +
                    /note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          complement(4093..4115)
 +
                    /label="pSB1A3 SapI rev"
 +
                    /note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          2002..2019
 +
                    /label="pSB1A3 fwd SapI"
 +
                    /note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          2002..2023
 +
                    /label="Linear pSB1A3 BsmBI Fwd"
 +
                    /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
ORIGIN
 +
        1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
 +
      61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
 +
      121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
 +
      181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
 +
      241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
 +
      301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
 +
      361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
 +
      421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
 +
      481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
 +
      541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
 +
      601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
 +
      661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
 +
      721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
 +
      781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
 +
      841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
 +
      901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
 +
      961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
 +
    1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
 +
    1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
 +
    1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
 +
    1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
 +
    1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
 +
    1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
 +
    1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
 +
    1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
 +
    1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
 +
    1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
 +
    1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
 +
    1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
 +
    1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
 +
    1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
 +
    1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
 +
    1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
 +
    1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
 +
    2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
 +
    2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
 +
    2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
 +
    2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
 +
    2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
 +
    2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
 +
    2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
 +
    2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
 +
    2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
 +
    2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
 +
    2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
 +
    2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
 +
    2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
 +
    2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
 +
    2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
 +
    2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
 +
    3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
 +
    3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
 +
    3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
 +
    3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
 +
    3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
 +
    3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
 +
    3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
 +
    3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
 +
    3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
 +
    3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
 +
    3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
 +
    3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
 +
    3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
 +
    3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
 +
    3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
 +
    3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
 +
    3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
 +
    4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
 +
    4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
 +
    4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
 +
    4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
 +
    4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
 +
    4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
 +
    4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
 +
    4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
 +
    4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
 +
    4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
 +
    4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
 +
    4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
 +
    4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
 +
    4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
 +
    4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
 +
    4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
 +
    4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
 +
    5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
 +
    5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
 +
    5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
 +
    5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
 +
//`
 +
       
 +
sequences.seq_Linear_pSB1A3_BsmBI_Fwd = {};
 +
sequences.seq_Linear_pSB1A3_BsmBI_Fwd.varName = "seq_Linear_pSB1A3_BsmBI_Fwd";
 +
sequences.seq_Linear_pSB1A3_BsmBI_Fwd.name = "Linear pSB1A3 BsmBI Fwd";
 +
sequences.seq_Linear_pSB1A3_BsmBI_Fwd.seqType = "linear";
 +
sequences.seq_Linear_pSB1A3_BsmBI_Fwd.comp = false;
 +
sequences.seq_Linear_pSB1A3_BsmBI_Fwd.data = `LOCUS      Linear_pSB1A3_BsmBI_Fwd  31 bp ds-DNA    linear      20-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 CTATTTCGTC TCCagtccgg caaaaaaggg c
 +
//`
 +
       
 +
sequences.seq_Linear_pSB1A3_BsmBI_Rev = {};
 +
sequences.seq_Linear_pSB1A3_BsmBI_Rev.varName = "seq_Linear_pSB1A3_BsmBI_Rev";
 +
sequences.seq_Linear_pSB1A3_BsmBI_Rev.name = "Linear pSB1A3 BsmBI Rev";
 +
sequences.seq_Linear_pSB1A3_BsmBI_Rev.seqType = "linear";
 +
sequences.seq_Linear_pSB1A3_BsmBI_Rev.comp = false;
 +
sequences.seq_Linear_pSB1A3_BsmBI_Rev.data = `LOCUS      Linear_pSB1A3_BsmBI_Rev  36 bp ds-DNA    linear      20-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ATTCCCCGTC TCCcagaaat catccttagc gaaagc
 +
//`
 +
       
 +
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites = {};
 +
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.varName = "seq_Linear_pSB1A3_with_BsmBI_Sites";
 +
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.name = "Linear pSB1A3 with BsmBI Sites";
 +
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.seqType = "linear";
 +
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.comp = true;
 +
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.data = `LOCUS      Linearized_pSB1A3_w/_Bs 2140 bp ds-DNA    linear      24-JUN-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_120731_229341"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    CDS            complement(1027..1887)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind    1650..1669
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    primer_bind    complement(97..114)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer_bind    2060..2078
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind    complement(348..367)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature    14..2127
 +
                    /label="pSB1A3 Region"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature    1168..1168
 +
                    /label="Point Mutation"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      2082..2125
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    terminator      16..73
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    promoter        complement(1888..1992)
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    rep_origin      complement(268..856)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(2100..2127)
 +
                    /label="BBa_Prefix & Promoter SDM Rev"
 +
                    /note="sequence: cagaaatcatccttagcgaaagctaagg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          14..35
 +
                    /label="Linear pSB1A3 BsmBI Fwd"
 +
                    /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
ORIGIN
 +
        1 CTATTTCGTC TCCagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
 +
      61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
 +
      121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
 +
      181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
 +
      241 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
 +
      301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
 +
      361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
 +
      421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
 +
      481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
 +
      541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
 +
      601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
 +
      661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
 +
      721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
 +
      781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
 +
      841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
 +
      901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
 +
      961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
 +
    1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
 +
    1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
 +
    1141 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc
 +
    1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
 +
    1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
 +
    1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
 +
    1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
 +
    1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
 +
    1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
 +
    1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
 +
    1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
 +
    1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
 +
    1741 gagatccagt tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt
 +
    1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
 +
    1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
 +
    1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
 +
    1981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
 +
    2041 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
 +
    2101 cttagctttc gctaaggatg atttctgGGA GACGGGGAAT
 +
//`
 +
       
 +
sequences.seq_pAC4CL1_FWD = {};
 +
sequences.seq_pAC4CL1_FWD.varName = "seq_pAC4CL1_FWD";
 +
sequences.seq_pAC4CL1_FWD.name = "pAC-4CL1 FWD";
 +
sequences.seq_pAC4CL1_FWD.seqType = "linear";
 +
sequences.seq_pAC4CL1_FWD.comp = false;
 +
sequences.seq_pAC4CL1_FWD.data = `LOCUS      pAC-4CL1_FWD              39 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TTCCCAGCTC TTCCagtatg gaggaggatt acaaaatgg
 +
//`
 +
       
 +
sequences.seq_pAC4CL1_REV = {};
 +
sequences.seq_pAC4CL1_REV.varName = "seq_pAC4CL1_REV";
 +
sequences.seq_pAC4CL1_REV.name = "pAC-4CL1 REV";
 +
sequences.seq_pAC4CL1_REV.seqType = "linear";
 +
sequences.seq_pAC4CL1_REV.comp = false;
 +
sequences.seq_pAC4CL1_REV.data = `LOCUS      pAC-4CL1_REV              39 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TTCCCAGCTC TTCCcaatca caatccattt gctagtttt
 +
//`
 +
       
 +
sequences.seq_pAC4CL1 = {};
 +
sequences.seq_pAC4CL1.varName = "seq_pAC4CL1";
 +
sequences.seq_pAC4CL1.name = "pAC-4CL1";
 +
sequences.seq_pAC4CL1.seqType = "both";
 +
sequences.seq_pAC4CL1.comp = true;
 +
sequences.seq_pAC4CL1.data = `LOCUS      pAC-4CL1                6156 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
KEYWORDS    "marker:CmR" "ref:pmid\:16551366"                                 
 +
FEATURES            Location/Qualifiers
 +
    CDS            complement(join(5716..6156,1..219))
 +
                    /label="CmR"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    promoter        1969..1975
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    protein_bind    1909..1930
 +
                    /label="CAP binding site"
 +
                    /ApEinfo_revcolor=#31849b
 +
                    /ApEinfo_fwdcolor=#31849b
 +
    CDS            complement(join(5719..6156,1..219))
 +
                    /label="CmR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    promoter        1504..1509
 +
                    /label="tet promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    rep_origin      complement(848..1392)
 +
                    /label="p15A ori"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    promoter        1951..1968
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    2016..3719
 +
                    /label="4CL"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    promoter        1527..1532
 +
                    /label="tet promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter        1945..1950
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter        complement(220..322)
 +
                    /label="cat promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter        1510..1526
 +
                    /label="tet promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter        1945..1975
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
ORIGIN
 +
        1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
 +
      61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
 +
      121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
 +
      181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
 +
      241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
 +
      301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
 +
      361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
 +
      421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
 +
      481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
 +
      541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
 +
      601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
 +
      661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
 +
      721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
 +
      781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
 +
      841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
 +
      901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
 +
      961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
 +
    1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
 +
    1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
 +
    1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
 +
    1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
 +
    1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
 +
    1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
 +
    1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttcaagat ttcagtgcaa
 +
    1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
 +
    1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
 +
    1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
 +
    1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
 +
    1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
 +
    1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
 +
    1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
 +
    1861 cgtcctgtgg atccccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
 +
    1921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
 +
    1981 aattgtgagc gtctagagcg cccggggaat tccccatgga ggaggattac aaaatggcgc
 +
    2041 cacaagaaca agcagtttct caggtgatgg agaaacagag caacaacaac aacagtgacg
 +
    2101 tcattttccg atcaaagtta ccggatattt acatcccgaa ccacctatct ctccacgact
 +
    2161 acatcttcca aaacatctcc gaattcgcca ctaagccttg cctaatcaac ggaccaaccg
 +
    2221 gccacgtgta cacttactcc gacgtccacg tcatctcccg ccaaatcgcc gccaattttc
 +
    2281 acaaactcgg cgttaaccaa aacgacgtcg tcatgctcct cctcccaaac tgtcccgaat
 +
    2341 tcgtcctctc tttcctcgcc gcctccttcc gcggcgcaac cgccaccgcc gcaaaccctt
 +
    2401 tcttcactcc ggcggagata gctaaacaag ccaaagcctc caacaccaaa ctcataatca
 +
    2461 ccgaagctcg ttacgtcgac aaaatcaaac cacttcaaaa cgacgacgga gtagtcatcg
 +
    2521 tctgcatcga cgacaacgaa tccgtgccaa tccctgaagg ctgcctccgc ttcaccgagt
 +
    2581 tgactcagtc gacaaccgag gcatcagaag tcatcgactc ggtggagatt tcaccggacg
 +
    2641 acgtggtggc actaccttac tcctctggca cgacgggatt accaaaagga gtgatgctga
 +
    2701 ctcacaaggg actagtcacg agcgttgctc agcaagtcga cggcgagaac ccgaatcttt
 +
    2761 atttccacag cgatgacgtc atactctgtg ttttgcccat gtttcatatc tacgctttga
 +
    2821 actcgatcat gttgtgtggt cttagagttg gtgcggcgat tctgataatg ccgaagtttg
 +
    2881 agatcaatct gctattggag ctgatccaga ggtgtaaagt gacggtggct ccgatggttc
 +
    2941 cgccgattgt gttggccatt gcgaagtctt cggagacgga gaagtatgat ttgagctcga
 +
    3001 taagagtggt gaaatctggt gctgctcctc ttggtaaaga acttgaagat gccgttaatg
 +
    3061 ccaagtttcc taatgccaaa ctcggtcagg gatacggaat gacggaagca ggtccagtgc
 +
    3121 tagcaatgtc gttaggtttt gcaaaggaac cttttccggt taagtcagga gcttgtggta
 +
    3181 ctgttgtaag aaatgctgag atgaaaatag ttgatccaga caccggagat tctctttcga
 +
    3241 ggaatcaacc cggtgagatt tgtattcgtg gtcaccagat catgaaaggt tacctcaaca
 +
    3301 atccggcagc tacagcagag accattgata aagacggttg gcttcatact ggagatattg
 +
    3361 gattgatcga tgacgatgac gagcttttca tcgttgatcg attgaaagaa cttatcaagt
 +
    3421 ataaaggttt tcaggtagct ccggctgagc tagaggcttt gctcatcggt catcctgaca
 +
    3481 ttactgatgt tgctgttgtc gcaatgaaag aagaagcagc tggtgaagtt cctgttgcat
 +
    3541 ttgtggtgaa atcgaaggat tcggagttat cagaagatga tgtgaagcaa ttcgtgtcga
 +
    3601 aacaggttgt gttttacaag agaatcaaca aagtgttctt cactgaatcc attcctaaag
 +
    3661 ctccatcagg gaagatattg aggaaagatc tgagggcaaa actagcaaat ggattgtgac
 +
    3721 catgggcggc cgctgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
 +
    3781 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
 +
    3841 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
 +
    3901 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
 +
    3961 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
 +
    4021 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
 +
    4081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
 +
    4141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
 +
    4201 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
 +
    4261 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
 +
    4321 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
 +
    4381 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
 +
    4441 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
 +
    4501 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
 +
    4561 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
 +
    4621 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
 +
    4681 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
 +
    4741 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
 +
    4801 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
 +
    4861 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
 +
    4921 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
 +
    4981 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
 +
    5041 gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga
 +
    5101 taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt
 +
    5161 acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt
 +
    5221 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc
 +
    5281 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac
 +
    5341 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
 +
    5401 tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata
 +
    5461 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
 +
    5521 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
 +
    5581 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
 +
    5641 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
 +
    5701 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
 +
    5761 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
 +
    5821 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
 +
    5881 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
 +
    5941 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
 +
    6001 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
 +
    6061 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
 +
    6121 ccatatcacc agctcaccgt ctttcattgc catacg
 +
//`
 +
       
 +
sequences.seq_pduABJKNUT_Fwd = {};
 +
sequences.seq_pduABJKNUT_Fwd.varName = "seq_pduABJKNUT_Fwd";
 +
sequences.seq_pduABJKNUT_Fwd.name = "pduABJKNUT Fwd";
 +
sequences.seq_pduABJKNUT_Fwd.seqType = "linear";
 +
sequences.seq_pduABJKNUT_Fwd.comp = false;
 +
sequences.seq_pduABJKNUT_Fwd.data = `LOCUS      pduABJKNUT_Fwd            46 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCTGCTCGTC TCTgaccaca acggtttccc tgtagaaata attttg
 +
//`
 +
       
 +
sequences.seq_pduABJKNUT_promoter = {};
 +
sequences.seq_pduABJKNUT_promoter.varName = "seq_pduABJKNUT_promoter";
 +
sequences.seq_pduABJKNUT_promoter.name = "pduABJKNUT promoter";
 +
sequences.seq_pduABJKNUT_promoter.seqType = "linear";
 +
sequences.seq_pduABJKNUT_promoter.comp = true;
 +
sequences.seq_pduABJKNUT_promoter.data = `LOCUS      *pduABJKNUT_promoter    133 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    38..72
 +
                    /label="BBa_J23114"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    34..37
 +
                    /label="Overhang pSB1C3"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    97..100
 +
                    /label="Overhang pduABJKNUT"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    misc_binding    74..96
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 AAGTGTTCGA ACGCTTCAGA TCTGTACGTC TCACAACttt atggctagct cagtcctagg
 +
      61 tacaatgcta gcTGGAATTG TGAGCGGATA ACAATTGACC AGAGACGTCT GCTTGCCAAC
 +
      121 CGTAGAACTT ACG
 +
//`
 +
       
 +
sequences.seq_pduABJKNUT_Rev = {};
 +
sequences.seq_pduABJKNUT_Rev.varName = "seq_pduABJKNUT_Rev";
 +
sequences.seq_pduABJKNUT_Rev.name = "pduABJKNUT Rev";
 +
sequences.seq_pduABJKNUT_Rev.seqType = "linear";
 +
sequences.seq_pduABJKNUT_Rev.comp = false;
 +
sequences.seq_pduABJKNUT_Rev.data = `LOCUS      pduABJKNUT_Rev            42 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ACTGTTCGTC TCAgctttgt tagcagccgg atccaaaagt ag
 +
//`
 +
       
 +
sequences.seq_PduD_final_product = {};
 +
sequences.seq_PduD_final_product.varName = "seq_PduD_final_product";
 +
sequences.seq_PduD_final_product.name = "PduD (final product)";
 +
sequences.seq_PduD_final_product.seqType = "linear";
 +
sequences.seq_PduD_final_product.comp = true;
 +
sequences.seq_PduD_final_product.data = `LOCUS      *PduD_(final_product)    658 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    115..154
 +
                    /label="random bp"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    18..112
 +
                    /label="BBa_B0014 Double Terminator"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    653..658
 +
                    /label="rando runway"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    218..231
 +
                    /label="Anderson RBS 7"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    Scar            59..66
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    583..621
 +
                    /label="BBa_B1006"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    1..6
 +
                    /label="Rando Runway"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    286..582
 +
                    /label="Spacer + ZFc"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    misc_feature    14..17
 +
                    /label="5' overhang with pdu"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    159..193
 +
                    /label="J23101 Anderson Promoter"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    232..285
 +
                    /label="pduD (1-18 aa)"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_binding    195..217
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 TGAGTACGTC TCAAAGCtca cactggctca ccttcgggtg ggcctttctg cgtttatata
 +
      61 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tttgAAGGGA
 +
      121 GTAAGTCTTC GAATGGTTCA GTTATAACTA TATAgctgtt tacagctagc tcagtcctag
 +
      181 gtattatgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGAGACT CATGGAAATC
 +
      241 AACGAAAAAC TGCTGCGTCA GATTATTGAG GACGTGTTAC GCGATACGTC GGCGGCCGCA
 +
      301 CCAGGCGAAC GCCCATTTCA GTGTCGCATC TGCATGCGTA ATTTCTCTTC ACCGAGCAAA
 +
      361 CTTATTCGCC ATACCCGTAC CCATACTGGC GAGAAACCCT TTCAGTGTCG TATCTGTATG
 +
      421 CGAAACTTCT CCGACGGGAG CAACCTGGCT CGGCACTTGC GTACACATAC CGGTGAAAAG
 +
      481 CCGTTTCAAT GCCGTATTTG CATGCGCAAT TTCAGTCGCG TGGATAACTT ACCGAGACAC
 +
      541 CTGAAAACGC ACTACCCGTA TGATGTTCCT GATTATGCGT AAaaaaaaaa accccgcccc
 +
      601 tgacagggcg gggttttttt tAATTAAAGA CACAAGTTTC CGTCAAGAGA CGTCGAGT
 +
//`
 +
       
 +
sequences.seq_pduD_on_pSB1C3__Final_Part = {};
 +
sequences.seq_pduD_on_pSB1C3__Final_Part.varName = "seq_pduD_on_pSB1C3__Final_Part";
 +
sequences.seq_pduD_on_pSB1C3__Final_Part.name = "pduD on pSB1C3 - Final Part";
 +
sequences.seq_pduD_on_pSB1C3__Final_Part.seqType = "both";
 +
sequences.seq_pduD_on_pSB1C3__Final_Part.comp = true;
 +
sequences.seq_pduD_on_pSB1C3__Final_Part.data = `LOCUS      pduD_on_pSB1C3_-_Final_ 2589 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    primer_bind    complement(333..352)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    Biobricking    2569..2584
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    2028..2028
 +
                    /label="BioBrick prefix"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    misc_feature    2177..2473
 +
                    /label="Spacer + ZFc"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer_bind    complement(82..99)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    source          1..2028
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2050..2084
 +
                    /label="new promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    terminator      1982..2025
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    Scar            2515..2522
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    rep_origin      complement(253..841)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    CDS            complement(1139..1798)
 +
                    /label="CmR"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    join(2585..2589,1..2028)
 +
                    /label="pSB1C3 Backbone Region"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    misc_feature    2585..2589
 +
                    /label="BioBrick suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    terminator      1..58
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    primer_bind    1713..1732
 +
                    /label="CAT-R"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    2123..2176
 +
                    /label="pduD (1-18 aa)"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Biobricking    2029..2049
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind    1960..1978
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    misc_feature    2474..2568
 +
                    /label="BBa_B0014 Double Terminator"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    Biobricking    2569..2589
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    terminator      1024..1118
 +
                    /label="lambda t0 terminator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    Biobricking    2028..2049
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    source          2585..2589
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2109..2122
 +
                    /label="RBS-J61107"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
 +
      61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
 +
      121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
 +
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
 +
      241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
 +
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
 +
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
 +
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
 +
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
 +
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
 +
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
 +
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
 +
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
 +
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
 +
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
 +
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
 +
      961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
 +
    1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
 +
    1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
 +
    1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
 +
    1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
 +
    1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
 +
    1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
 +
    1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
 +
    1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
 +
    1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
 +
    1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
 +
    1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
 +
    1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
 +
    1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
 +
    1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
 +
    1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
 +
    1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
 +
    1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
 +
    2041 cttctagagt ttacagctag ctcagtccta ggtattatgc tagcTGGAAT TGTGAGCGGA
 +
    2101 TAACAATTGA AAGAAGAGAC TCATGGAAAT CAACGAAAAA CTGCTGCGTC AGATTATTGA
 +
    2161 GGACGTGTTA CGCGATACGT CGGCGGCCGC ACCAGGCGAA CGCCCATTTC AGTGTCGCAT
 +
    2221 CTGCATGCGT AATTTCTCTT CACCGAGCAA ACTTATTCGC CATACCCGTA CCCATACTGG
 +
    2281 CGAGAAACCC TTTCAGTGTC GTATCTGTAT GCGAAACTTC TCCGACGGGA GCAACCTGGC
 +
    2341 TCGGCACTTG CGTACACATA CCGGTGAAAA GCCGTTTCAA TGCCGTATTT GCATGCGCAA
 +
    2401 TTTCAGTCGC GTGGATAACT TACCGAGACA CCTGAAAACG CACTACCCGT ATGATGTTCC
 +
    2461 TGATTATGCG TAAtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
 +
    2521 agagagaata taaaaagcca gattattaat ccggcttttt tattatttta ctagtagcgg
 +
    2581 ccgctgcag
 +
//`
 +
       
 +
sequences.seq_pHIV_Backbone_NEB_FWD = {};
 +
sequences.seq_pHIV_Backbone_NEB_FWD.varName = "seq_pHIV_Backbone_NEB_FWD";
 +
sequences.seq_pHIV_Backbone_NEB_FWD.name = "pHIV Backbone NEB FWD";
 +
sequences.seq_pHIV_Backbone_NEB_FWD.seqType = "linear";
 +
sequences.seq_pHIV_Backbone_NEB_FWD.comp = false;
 +
sequences.seq_pHIV_Backbone_NEB_FWD.data = `LOCUS      pHIV_Backbone_NEB_FWD    42 bp ds-DNA    linear      02-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 taaagttctg taatattagt gaaagaggag aaatactaga tg
 +
//`
 +
       
 +
sequences.seq_pHIV_Backbone_NEB_REV = {};
 +
sequences.seq_pHIV_Backbone_NEB_REV.varName = "seq_pHIV_Backbone_NEB_REV";
 +
sequences.seq_pHIV_Backbone_NEB_REV.name = "pHIV Backbone NEB REV";
 +
sequences.seq_pHIV_Backbone_NEB_REV.seqType = "linear";
 +
sequences.seq_pHIV_Backbone_NEB_REV.comp = false;
 +
sequences.seq_pHIV_Backbone_NEB_REV.data = `LOCUS      pHIV_Backbone_NEB_REV    22 bp ds-DNA    linear      08-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 agtgccagat gaattgcctg ta
 +
//`
 +
       
 +
sequences.seq_pHIV_GGA_FWD = {};
 +
sequences.seq_pHIV_GGA_FWD.varName = "seq_pHIV_GGA_FWD";
 +
sequences.seq_pHIV_GGA_FWD.name = "pHIV GGA FWD";
 +
sequences.seq_pHIV_GGA_FWD.seqType = "linear";
 +
sequences.seq_pHIV_GGA_FWD.comp = false;
 +
sequences.seq_pHIV_GGA_FWD.data = `LOCUS      pHIV_GGA_FWD              34 bp ds-DNA    linear      07-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 CTACTACGTC TCTGGAtaga tgccgattag cccg
 +
//`
 +
       
 +
sequences.seq_pHIV_GGA_REV = {};
 +
sequences.seq_pHIV_GGA_REV.varName = "seq_pHIV_GGA_REV";
 +
sequences.seq_pHIV_GGA_REV.name = "pHIV GGA REV";
 +
sequences.seq_pHIV_GGA_REV.seqType = "linear";
 +
sequences.seq_pHIV_GGA_REV.comp = false;
 +
sequences.seq_pHIV_GGA_REV.data = `LOCUS      pHIV_GGA_REV              37 bp ds-DNA    linear      02-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCAGTACGTC TCAgccgctt ctagtatata aacgcag
 +
//`
 +
       
 +
sequences.seq_pHIV_NEB_FWD = {};
 +
sequences.seq_pHIV_NEB_FWD.varName = "seq_pHIV_NEB_FWD";
 +
sequences.seq_pHIV_NEB_FWD.name = "pHIV NEB FWD";
 +
sequences.seq_pHIV_NEB_FWD.seqType = "linear";
 +
sequences.seq_pHIV_NEB_FWD.comp = false;
 +
sequences.seq_pHIV_NEB_FWD.data = `LOCUS      pHIV_NEB_FWD              22 bp ds-DNA    linear      08-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 caggcaattc atctggcact ac
 +
//`
 +
       
 +
sequences.seq_pHIV_NEB_REV = {};
 +
sequences.seq_pHIV_NEB_REV.varName = "seq_pHIV_NEB_REV";
 +
sequences.seq_pHIV_NEB_REV.name = "pHIV NEB REV";
 +
sequences.seq_pHIV_NEB_REV.seqType = "linear";
 +
sequences.seq_pHIV_NEB_REV.comp = false;
 +
sequences.seq_pHIV_NEB_REV.data = `LOCUS      pHIV_NEB_REV              37 bp ds-DNA    linear      08-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 tcctctttca ctaatattac agaactttac gaatacc
 +
//`
 +
       
 +
sequences.seq_pHIV_pTp66p51_Linearized = {};
 +
sequences.seq_pHIV_pTp66p51_Linearized.varName = "seq_pHIV_pTp66p51_Linearized";
 +
sequences.seq_pHIV_pTp66p51_Linearized.name = "pHIV_pTp66p51 Linearized";
 +
sequences.seq_pHIV_pTp66p51_Linearized.seqType = "linear";
 +
sequences.seq_pHIV_pTp66p51_Linearized.comp = true;
 +
sequences.seq_pHIV_pTp66p51_Linearized.data = `LOCUS      pHIV_pTp66p51_Linearize 3226 bp ds-DNA    linear      07-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    1732..3057
 +
                    /label="HIVRT P51"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    terminator      3167..3194
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    Scar            3064..3071
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    20..1705
 +
                    /label="HIVRT p66"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    RBS            1714..1725
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    Scar            3152..3159
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      3080..3151
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    primer          complement(1458..1479)
 +
                    /label="pHIV Backbone NEB REV"
 +
                    /note="sequence: agtgccagatgaattgcctgta"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          1726..1746
 +
                    /label="pHIV GGA FWD"
 +
                    /note="sequence: CTACTACGTCTCTGGAtagatgccgattagcccg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(3179..3194)
 +
                    /label="pHIV GGA REV"
 +
                    /note="sequence: TCAGTACGTCTCAcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(3179..3194)
 +
                    /label="pHIV REV"
 +
                    /note="sequence: AATATCCGTCTCTAGTCcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          5..25
 +
                    /label="pHIV GGA FWD"
 +
                    /note="sequence: CTACTACGTCTCTaaagaggagaaatactagatgccg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          5..34
 +
                    /label="pHIV GGA FWD"
 +
                    /note="sequence: CTACTACGTCTCTGGAtagatgccgattagcccg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          1702..1737
 +
                    /label="pHIV Backbone NEB FWD"
 +
                    /note="sequence: gtaatattagtgaaagaggagaaatactagatgccg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(1685..1721)
 +
                    /label="pHIV NEB REV"
 +
                    /note="sequence: tcctctttcactaatattacagaactttacgaatacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          1460..1481
 +
                    /label="pHIV NEB FWD"
 +
                    /note="sequence: caggcaattcatctggcactac"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(3179..3194)
 +
                    /label="pHIV REV"
 +
                    /note="sequence: ATTACACGTCTCAcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
ORIGIN
 +
        1 CTACTACGTC TCTGGATaga tgccgattag cccgattgaa accgttccgg ttaaactgaa
 +
      61 accgggtatg gatggtccga aagttaaaca gtggcctctg accgaagaaa aaatcaaagc
 +
      121 actggttgaa atctgcaccg agatggaaaa agaaggcaaa attagcaaaa tcggtccgga
 +
      181 aaatccgtat aatacaccgg tttttgccat taagaaaaaa gatagcacca aatggcgcaa
 +
      241 actggtggat tttcgtgaac tgaataaacg cacccaggat ttttgggaag ttcagctggg
 +
      301 tattccgcat ccggcaggtc tgaaacagaa aaaaagcgtt accgttctgg atgttggtga
 +
      361 tgcatatttt agcgttccgc tggataaaga tttccgtaaa tataccgcat ttaccatccc
 +
      421 gagcattaat aacgaaacac cgggtattcg ctatcagtat aatgttctgc cgcagggttg
 +
      481 gaaaggtagt ccggcaattt ttcagtgtag catgaccaaa attctggaac cgtttcgtaa
 +
      541 acagaatccg gatattgtga tctaccagta tatggatgat ctgtatgttg gtagcgatct
 +
      601 ggaaattggt cagcatcgta ccaaaattga agaactgcgt cagcatctgc tgcgttgggg
 +
      661 ttttaccaca ccggataaaa aacatcagaa agaaccgcct tttctgtgga tgggttatga
 +
      721 actgcatccg gataaatgga ccgttcagcc gattgttctg ccggaaaaag atagctggac
 +
      781 cgttaatgat attcagaaac tggtgggtaa actgaattgg gcaagccaga tttatgccgg
 +
      841 tattaaagtt cgtcagctgt gtaaactgct gcgtggcacc aaagcactga ccgaagttgt
 +
      901 tccgctgaca gaagaagcag aactggaact ggcagaaaat cgtgaaattc tgaaagaacc
 +
      961 ggttcacggc gtttattatg atccgagcaa agatctgatt gccgaaattc agaaacaggg
 +
    1021 tcagggtcag tggacctatc agatttatca agaaccgttt aaaaacctga aaaccggcaa
 +
    1081 atatgcacgt atgaaaggtg cacataccaa cgatgttaaa cagctgaccg aagcagttca
 +
    1141 gaaaattgca accgaaagca ttgtgatttg gggtaaaacc ccgaaattca aactgccgat
 +
    1201 tcagaaagaa acctgggaag catggtggac cgaatattgg caggcaacct ggattccgga
 +
    1261 atgggaattt gttaataccc ctccgctggt taaactgtgg tatcagctgg aaaaagaacc
 +
    1321 gattattggt gccgaaacct tttatgttga tggtgcagcc aatcgtgaaa ccaaactggg
 +
    1381 taaagcaggt tatgttaccg atcgtggtcg tcagaaagtg gtgccgctga ccgataccac
 +
    1441 caatcagaaa accgaactac aggcaattca tctggcacta caggatagcg gtctggaagt
 +
    1501 taatattgtt accgatagcc agtatgccct gggtattatt caggcacagc cggataaaag
 +
    1561 cgaaagcgaa ctggttagcc agattattga acagctgatc aaaaaagaaa aagtgtacct
 +
    1621 ggcatgggtt ccggcacata aaggtattgg tggtaatgaa caggttgatg gtctggttag
 +
    1681 cgcaggtatt cgtaaagttc tgtaatatta gtgaaagagg agaaatacta gatgccgatt
 +
    1741 agcccgattg aaaccgttcc ggttaaactg aaaccgggta tggatggtcc gaaagttaaa
 +
    1801 cagtggcctc tgaccgaaga aaaaatcaaa gcactggttg aaatctgcac cgagatggaa
 +
    1861 aaagaaggca aaattagcaa aatcggtccg gaaaatccgt ataatacacc ggtttttgcc
 +
    1921 attaagaaaa aagatagcac caaatggcgc aaactggtgg attttcgtga actgaataaa
 +
    1981 cgcacccagg atttttggga agttcagctg ggtattccgc atccggcagg tctgaaacag
 +
    2041 aaaaaaagcg ttaccgttct ggatgttggt gatgcatatt ttagcgttcc gctggataaa
 +
    2101 gatttccgta aatataccgc atttaccatc ccgagcatta ataacgaaac accgggtatt
 +
    2161 cgctatcagt ataatgttct gccgcagggt tggaaaggta gtccggcaat ttttcagtgt
 +
    2221 agcatgacca aaattctgga accgtttcgt aaacagaatc cggatattgt gatctaccag
 +
    2281 tatatggatg atctgtatgt tggtagcgat ctggaaattg gtcagcatcg taccaaaatt
 +
    2341 gaagaactgc gtcagcatct gctgcgttgg ggttttacca caccggataa aaaacatcag
 +
    2401 aaagaaccgc cttttctgtg gatgggttat gaactgcatc cggataaatg gaccgttcag
 +
    2461 ccgattgttc tgccggaaaa agatagctgg accgttaatg atattcagaa actggtgggt
 +
    2521 aaactgaatt gggcaagcca gatttatgcc ggtattaaag ttcgtcagct gtgtaaactg
 +
    2581 ctgcgtggca ccaaagcact gaccgaagtt gttccgctga cagaagaagc agaactggaa
 +
    2641 ctggcagaaa atcgtgaaat tctgaaagaa ccggttcacg gcgtttatta tgatccgagc
 +
    2701 aaagatctga ttgccgaaat tcagaaacag ggtcagggtc agtggaccta tcagatttat
 +
    2761 caagaaccgt ttaaaaacct gaaaaccggc aaatatgcac gtatgaaagg tgcacatacc
 +
    2821 aacgatgtta aacagctgac cgaagcagtt cagaaaattg caaccgaaag cattgtgatt
 +
    2881 tggggtaaaa ccccgaaatt caaactgccg attcagaaag aaacctggga agcatggtgg
 +
    2941 accgaatatt ggcaggcaac ctggattccg gaatgggaat ttgttaatac ccctccgctg
 +
    3001 gttaaactgt ggtatcagct ggaaaaagaa ccgattattg gtgccgaaac cttttaagat
 +
    3061 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
 +
    3121 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
 +
    3181 tgggcctttc tgcgtttata tactagaagc ggcTGAGACG TACTGA
 +
//`
 +
       
 +
sequences.seq_pHIV_pTp66p51_NEBuilder_Product = {};
 +
sequences.seq_pHIV_pTp66p51_NEBuilder_Product.varName = "seq_pHIV_pTp66p51_NEBuilder_Product";
 +
sequences.seq_pHIV_pTp66p51_NEBuilder_Product.name = "pHIV_pTp66p51 NEBuilder Product";
 +
sequences.seq_pHIV_pTp66p51_NEBuilder_Product.seqType = "both";
 +
sequences.seq_pHIV_pTp66p51_NEBuilder_Product.comp = true;
 +
sequences.seq_pHIV_pTp66p51_NEBuilder_Product.data = `LOCUS      pHIV_pTp66p51_NEBuilder 6621 bp ds-DNA    circular    07-SEP-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_78233_145927"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    primer_bind    6305..6324
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    Scar            4677..4684
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    promoter        1400..1428
 +
                    /label="tac promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    source          1..6621
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    CDS            complement(5682..6542)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    protein_bind    1434..1453
 +
                    /label="lac operator (symmetric)"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer_bind    complement(4752..4769)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Scar            4589..4596
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    CDS            complement(183..1265)
 +
                    /label="lacI"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    terminator      91..120
 +
                    /label="T3Te terminator"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    primer_bind    1227..1246
 +
                    /label="LacI-R"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
    misc_feature    1545..3230
 +
                    /label="HIVRT p66"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    rep_origin      complement(4905..5587)
 +
                    /label="ColE1 origin"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    rep_origin      complement(4923..5511)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    1454..1538
 +
                    /label="on g-block 1"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    RBS            3239..3250
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    terminator      4692..4719
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    misc_feature    3257..4582
 +
                    /label="HIVRT P51"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_RNA        1469..1518
 +
                    /label="sTRSV HHRz"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    terminator      4605..4676
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    promoter        complement(join(6543..6621,1..26))
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    primer_bind    complement(5003..5022)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    RBS            1527..1538
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    CDS            complement(5685..6344)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer          complement(2983..3004)
 +
                    /label="pHIV Backbone NEB REV"
 +
                    /note="sequence: agtgccagatgaattgcctgta"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(4715..4738)
 +
                    /label="pHIV GGA REV"
 +
                    /note="sequence: TCAGTACGTCTCAgccgcttctagtatataaacgcag"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(3210..3246)
 +
                    /label="pHIV NEB REV"
 +
                    /note="sequence: tcctctttcactaatattacagaactttacgaatacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          1539..1559
 +
                    /label="pHIV GGA FWD"
 +
                    /note="sequence: CTACTACGTCTCTGGAtagatgccgattagcccg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          3218..3259
 +
                    /label="pHIV Backbone NEB FWD"
 +
                    /note="sequence: taaagttctgtaatattagtgaaagaggagaaatactagatg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          1469..1488
 +
                    /label="pHIV FWD"
 +
                    /note="sequence: ATTACACGTCTCActgtcaccggatgtgctttc"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          3227..3262
 +
                    /label="pHIV Backbone NEB FWD"
 +
                    /note="sequence: gtaatattagtgaaagaggagaaatactagatgccg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          1469..1488
 +
                    /label="pHIV FWD"
 +
                    /note="sequence: CGTGAGCGTCTCACGATctgtcaccggatgtgctttc"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(4704..4719)
 +
                    /label="pHIV REV"
 +
                    /note="sequence: AATATCCGTCTCTAGTCcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(4704..4719)
 +
                    /label="pHIV REV"
 +
                    /note="sequence: ATTACACGTCTCAcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          2985..3006
 +
                    /label="pHIV NEB FWD"
 +
                    /note="sequence: caggcaattcatctggcactac"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
 +
      61 gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc
 +
      121 agtgagttga ttgctacgta atgtcggcca attcgcgcta acttacatta attgcgttgc
 +
      181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
 +
      241 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
 +
      301 gagactggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
 +
      361 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
 +
      421 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
 +
      481 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
 +
      541 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
 +
      601 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
 +
      661 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
 +
      721 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg
 +
      781 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
 +
      841 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
 +
      901 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
 +
      961 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
 +
    1021 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
 +
    1081 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
 +
    1141 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
 +
    1201 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
 +
    1261 ttcatattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
 +
    1321 gttttgcgcc attcgatggc gcgccgcttc gtcaggccac atagctttct tgttctgatc
 +
    1381 ggaacgatcg ttggctgtgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
 +
    1441 agcgctcaca attctatgga ctatgtttct gtcaccggat gtgctttccg gtctgatgag
 +
    1501 tccgtgagga cgaaacagta ctagtgaaag aggagaaata ctagatgccg attagcccga
 +
    1561 ttgaaaccgt tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc
 +
    1621 ctctgaccga agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag
 +
    1681 gcaaaattag caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga
 +
    1741 aaaaagatag caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc
 +
    1801 aggatttttg ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa
 +
    1861 gcgttaccgt tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc
 +
    1921 gtaaatatac cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc
 +
    1981 agtataatgt tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga
 +
    2041 ccaaaattct ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg
 +
    2101 atgatctgta tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac
 +
    2161 tgcgtcagca tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac
 +
    2221 cgccttttct gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg
 +
    2281 ttctgccgga aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga
 +
    2341 attgggcaag ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg
 +
    2401 gcaccaaagc actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag
 +
    2461 aaaatcgtga aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc
 +
    2521 tgattgccga aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac
 +
    2581 cgtttaaaaa cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg
 +
    2641 ttaaacagct gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta
 +
    2701 aaaccccgaa attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat
 +
    2761 attggcaggc aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac
 +
    2821 tgtggtatca gctggaaaaa gaaccgatta ttggtgccga aaccttttat gttgatggtg
 +
    2881 cagccaatcg tgaaaccaaa ctgggtaaag caggttatgt taccgatcgt ggtcgtcaga
 +
    2941 aagtggtgcc gctgaccgat accaccaatc agaaaaccga actacaggca attcatctgg
 +
    3001 cactacagga tagcggtctg gaagttaata ttgttaccga tagccagtat gccctgggta
 +
    3061 ttattcaggc acagccggat aaaagcgaaa gcgaactggt tagccagatt attgaacagc
 +
    3121 tgatcaaaaa agaaaaagtg tacctggcat gggttccggc acataaaggt attggtggta
 +
    3181 atgaacaggt tgatggtctg gttagcgcag gtattcgtaa agttctgtaa tattagtgaa
 +
    3241 agaggagaaa tactagatgc cgattagccc gattgaaacc gttccggtta aactgaaacc
 +
    3301 gggtatggat ggtccgaaag ttaaacagtg gcctctgacc gaagaaaaaa tcaaagcact
 +
    3361 ggttgaaatc tgcaccgaga tggaaaaaga aggcaaaatt agcaaaatcg gtccggaaaa
 +
    3421 tccgtataat acaccggttt ttgccattaa gaaaaaagat agcaccaaat ggcgcaaact
 +
    3481 ggtggatttt cgtgaactga ataaacgcac ccaggatttt tgggaagttc agctgggtat
 +
    3541 tccgcatccg gcaggtctga aacagaaaaa aagcgttacc gttctggatg ttggtgatgc
 +
    3601 atattttagc gttccgctgg ataaagattt ccgtaaatat accgcattta ccatcccgag
 +
    3661 cattaataac gaaacaccgg gtattcgcta tcagtataat gttctgccgc agggttggaa
 +
    3721 aggtagtccg gcaatttttc agtgtagcat gaccaaaatt ctggaaccgt ttcgtaaaca
 +
    3781 gaatccggat attgtgatct accagtatat ggatgatctg tatgttggta gcgatctgga
 +
    3841 aattggtcag catcgtacca aaattgaaga actgcgtcag catctgctgc gttggggttt
 +
    3901 taccacaccg gataaaaaac atcagaaaga accgcctttt ctgtggatgg gttatgaact
 +
    3961 gcatccggat aaatggaccg ttcagccgat tgttctgccg gaaaaagata gctggaccgt
 +
    4021 taatgatatt cagaaactgg tgggtaaact gaattgggca agccagattt atgccggtat
 +
    4081 taaagttcgt cagctgtgta aactgctgcg tggcaccaaa gcactgaccg aagttgttcc
 +
    4141 gctgacagaa gaagcagaac tggaactggc agaaaatcgt gaaattctga aagaaccggt
 +
    4201 tcacggcgtt tattatgatc cgagcaaaga tctgattgcc gaaattcaga aacagggtca
 +
    4261 gggtcagtgg acctatcaga tttatcaaga accgtttaaa aacctgaaaa ccggcaaata
 +
    4321 tgcacgtatg aaaggtgcac ataccaacga tgttaaacag ctgaccgaag cagttcagaa
 +
    4381 aattgcaacc gaaagcattg tgatttgggg taaaaccccg aaattcaaac tgccgattca
 +
    4441 gaaagaaacc tgggaagcat ggtggaccga atattggcag gcaacctgga ttccggaatg
 +
    4501 ggaatttgtt aatacccctc cgctggttaa actgtggtat cagctggaaa aagaaccgat
 +
    4561 tattggtgcc gaaacctttt aagatcgcta ctagagccag gcatcaaata aaacgaaagg
 +
    4621 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
 +
    4681 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaagcggccg
 +
    4741 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
 +
    4801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
 +
    4861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
 +
    4921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
 +
    4981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
 +
    5041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 +
    5101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
 +
    5161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
 +
    5221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
 +
    5281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
 +
    5341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
 +
    5401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
 +
    5461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
 +
    5521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
 +
    5581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
 +
    5641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
 +
    5701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
 +
    5761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
 +
    5821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
 +
    5881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
 +
    5941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
 +
    6001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
 +
    6061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
 +
    6121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
 +
    6181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
 +
    6241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
 +
    6301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
 +
    6361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
 +
    6421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
 +
    6481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
 +
    6541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
 +
    6601 tacatatttg aatgtattta g
 +
//`
 +
       
 +
sequences.seq_pHIV_pTp66p51 = {};
 +
sequences.seq_pHIV_pTp66p51.varName = "seq_pHIV_pTp66p51";
 +
sequences.seq_pHIV_pTp66p51.name = "pHIV_pTp66p51";
 +
sequences.seq_pHIV_pTp66p51.seqType = "both";
 +
sequences.seq_pHIV_pTp66p51.comp = true;
 +
sequences.seq_pHIV_pTp66p51.data = `LOCUS      pHIV_pTp66p51          6621 bp ds-DNA    circular    09-OCT-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_78233_145927"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    primer_bind    complement(5003..5022)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    Scar            4677..4684
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind    1227..1246
 +
                    /label="LacI-R"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
    RBS            1527..1538
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    source          1..6621
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    1454..1526
 +
                    /label="on g-block 1"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind    complement(4752..4769)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    promoter        complement(join(6543..6621,1..26))
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    CDS            complement(5685..6344)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    terminator      4692..4719
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    misc_feature    3257..4582
 +
                    /label="HIVRT P51"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_RNA        1469..1518
 +
                    /label="sTRSV HHRz"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    RBS            3239..3250
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    CDS            complement(5682..6542)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    1545..3230
 +
                    /label="HIVRT p66"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    protein_bind    1434..1453
 +
                    /label="lac operator (symmetric)"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    Scar            4589..4596
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    rep_origin      complement(4923..5511)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    CDS            complement(183..1265)
 +
                    /label="lacI"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    rep_origin      complement(4905..5587)
 +
                    /label="ColE1 origin"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    promoter        1400..1428
 +
                    /label="tac promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind    6305..6324
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    terminator      91..120
 +
                    /label="T3Te terminator"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    terminator      4605..4676
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    primer          2985..3006
 +
                    /label="pHIV NEB FWD"
 +
                    /note="sequence: caggcaattcatctggcactac"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          1469..1488
 +
                    /label="pHIV FWD"
 +
                    /note="sequence: CGTGAGCGTCTCACGATctgtcaccggatgtgctttc"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          1517..1547
 +
                    /label="pHIV Backbone NEB FWD"
 +
                    /note="sequence: taaagttctgtaatattagtgaaagaggagaaatactagatg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(4704..4719)
 +
                    /label="pHIV REV"
 +
                    /note="sequence: ATTACACGTCTCAcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          3218..3259
 +
                    /label="pHIV Backbone NEB FWD"
 +
                    /note="sequence: taaagttctgtaatattagtgaaagaggagaaatactagatg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(4704..4719)
 +
                    /label="pHIV REV"
 +
                    /note="sequence: AATATCCGTCTCTAGTCcgcagaaaggcccacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(3210..3246)
 +
                    /label="pHIV NEB REV"
 +
                    /note="sequence: tcctctttcactaatattacagaactttacgaatacc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(2983..3004)
 +
                    /label="pHIV Backbone NEB REV"
 +
                    /note="sequence: agtgccagatgaattgcctgta"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          1469..1488
 +
                    /label="pHIV FWD"
 +
                    /note="sequence: ATTACACGTCTCActgtcaccggatgtgctttc"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
 +
      61 gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc
 +
      121 agtgagttga ttgctacgta atgtcggcca attcgcgcta acttacatta attgcgttgc
 +
      181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
 +
      241 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
 +
      301 gagactggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
 +
      361 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
 +
      421 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
 +
      481 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
 +
      541 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
 +
      601 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
 +
      661 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
 +
      721 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg
 +
      781 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
 +
      841 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
 +
      901 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
 +
      961 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
 +
    1021 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
 +
    1081 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
 +
    1141 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
 +
    1201 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
 +
    1261 ttcatattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
 +
    1321 gttttgcgcc attcgatggc gcgccgcttc gtcaggccac atagctttct tgttctgatc
 +
    1381 ggaacgatcg ttggctgtgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
 +
    1441 agcgctcaca attctatgga ctatgtttct gtcaccggat gtgctttccg gtctgatgag
 +
    1501 tccgtgagga cgaaacagta ctagtgaaag aggagaaata ctagatgccg attagcccga
 +
    1561 ttgaaaccgt tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc
 +
    1621 ctctgaccga agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag
 +
    1681 gcaaaattag caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga
 +
    1741 aaaaagatag caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc
 +
    1801 aggatttttg ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa
 +
    1861 gcgttaccgt tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc
 +
    1921 gtaaatatac cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc
 +
    1981 agtataatgt tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga
 +
    2041 ccaaaattct ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg
 +
    2101 atgatctgta tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac
 +
    2161 tgcgtcagca tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac
 +
    2221 cgccttttct gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg
 +
    2281 ttctgccgga aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga
 +
    2341 attgggcaag ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg
 +
    2401 gcaccaaagc actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag
 +
    2461 aaaatcgtga aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc
 +
    2521 tgattgccga aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac
 +
    2581 cgtttaaaaa cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg
 +
    2641 ttaaacagct gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta
 +
    2701 aaaccccgaa attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat
 +
    2761 attggcaggc aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac
 +
    2821 tgtggtatca gctggaaaaa gaaccgatta ttggtgccga aaccttttat gttgatggtg
 +
    2881 cagccaatcg tgaaaccaaa ctgggtaaag caggttatgt taccgatcgt ggtcgtcaga
 +
    2941 aagtggtgcc gctgaccgat accaccaatc agaaaaccga actgcaggca attcatctgg
 +
    3001 cactgcagga tagcggtctg gaagttaata ttgttaccga tagccagtat gccctgggta
 +
    3061 ttattcaggc acagccggat aaaagcgaaa gcgaactggt tagccagatt attgaacagc
 +
    3121 tgatcaaaaa agaaaaagtg tacctggcat gggttccggc acataaaggt attggtggta
 +
    3181 atgaacaggt tgatggtctg gttagcgcag gtattcgtaa agttctgtaa tactagtgaa
 +
    3241 agaggagaaa tactagatgc cgattagccc gattgaaacc gttccggtta aactgaaacc
 +
    3301 gggtatggat ggtccgaaag ttaaacagtg gcctctgacc gaagaaaaaa tcaaagcact
 +
    3361 ggttgaaatc tgcaccgaga tggaaaaaga aggcaaaatt agcaaaatcg gtccggaaaa
 +
    3421 tccgtataat acaccggttt ttgccattaa gaaaaaagat agcaccaaat ggcgcaaact
 +
    3481 ggtggatttt cgtgaactga ataaacgcac ccaggatttt tgggaagttc agctgggtat
 +
    3541 tccgcatccg gcaggtctga aacagaaaaa aagcgttacc gttctggatg ttggtgatgc
 +
    3601 atattttagc gttccgctgg ataaagattt ccgtaaatat accgcattta ccatcccgag
 +
    3661 cattaataac gaaacaccgg gtattcgcta tcagtataat gttctgccgc agggttggaa
 +
    3721 aggtagtccg gcaatttttc agtgtagcat gaccaaaatt ctggaaccgt ttcgtaaaca
 +
    3781 gaatccggat attgtgatct accagtatat ggatgatctg tatgttggta gcgatctgga
 +
    3841 aattggtcag catcgtacca aaattgaaga actgcgtcag catctgctgc gttggggttt
 +
    3901 taccacaccg gataaaaaac atcagaaaga accgcctttt ctgtggatgg gttatgaact
 +
    3961 gcatccggat aaatggaccg ttcagccgat tgttctgccg gaaaaagata gctggaccgt
 +
    4021 taatgatatt cagaaactgg tgggtaaact gaattgggca agccagattt atgccggtat
 +
    4081 taaagttcgt cagctgtgta aactgctgcg tggcaccaaa gcactgaccg aagttgttcc
 +
    4141 gctgacagaa gaagcagaac tggaactggc agaaaatcgt gaaattctga aagaaccggt
 +
    4201 tcacggcgtt tattatgatc cgagcaaaga tctgattgcc gaaattcaga aacagggtca
 +
    4261 gggtcagtgg acctatcaga tttatcaaga accgtttaaa aacctgaaaa ccggcaaata
 +
    4321 tgcacgtatg aaaggtgcac ataccaacga tgttaaacag ctgaccgaag cagttcagaa
 +
    4381 aattgcaacc gaaagcattg tgatttgggg taaaaccccg aaattcaaac tgccgattca
 +
    4441 gaaagaaacc tgggaagcat ggtggaccga atattggcag gcaacctgga ttccggaatg
 +
    4501 ggaatttgtt aatacccctc cgctggttaa actgtggtat cagctggaaa aagaaccgat
 +
    4561 tattggtgcc gaaacctttt aagatcgcta ctagagccag gcatcaaata aaacgaaagg
 +
    4621 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
 +
    4681 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaagcggccg
 +
    4741 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
 +
    4801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
 +
    4861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
 +
    4921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
 +
    4981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
 +
    5041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 +
    5101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
 +
    5161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
 +
    5221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
 +
    5281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
 +
    5341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
 +
    5401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
 +
    5461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
 +
    5521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
 +
    5581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
 +
    5641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
 +
    5701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
 +
    5761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
 +
    5821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
 +
    5881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
 +
    5941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
 +
    6001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
 +
    6061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
 +
    6121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
 +
    6181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
 +
    6241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
 +
    6301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
 +
    6361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
 +
    6421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
 +
    6481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
 +
    6541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
 +
    6601 tacatatttg aatgtattta g
 +
//`
 +
       
 +
sequences.seq_pMLRT_GGA_FWD = {};
 +
sequences.seq_pMLRT_GGA_FWD.varName = "seq_pMLRT_GGA_FWD";
 +
sequences.seq_pMLRT_GGA_FWD.name = "pMLRT GGA FWD";
 +
sequences.seq_pMLRT_GGA_FWD.seqType = "linear";
 +
sequences.seq_pMLRT_GGA_FWD.comp = false;
 +
sequences.seq_pMLRT_GGA_FWD.data = `LOCUS      pMLRT_GGA_FWD            29 bp ds-DNA    linear      07-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ATTACACGTC TCTCcaAggc ccgatcgat
 +
//`
 +
       
 +
sequences.seq_pMLRT_GGA_REV = {};
 +
sequences.seq_pMLRT_GGA_REV.varName = "seq_pMLRT_GGA_REV";
 +
sequences.seq_pMLRT_GGA_REV.name = "pMLRT GGA REV";
 +
sequences.seq_pMLRT_GGA_REV.seqType = "linear";
 +
sequences.seq_pMLRT_GGA_REV.comp = false;
 +
sequences.seq_pMLRT_GGA_REV.data = `LOCUS      pMLRT_GGA_REV            49 bp ds-DNA    linear      08-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCGCCTCGTC TCACCCTtct ggacatttat tatatataaa cgcagaaag
 +
//`
 +
       
 +
sequences.seq_pMLRT_Linearized = {};
 +
sequences.seq_pMLRT_Linearized.varName = "seq_pMLRT_Linearized";
 +
sequences.seq_pMLRT_Linearized.name = "pMLRT Linearized";
 +
sequences.seq_pMLRT_Linearized.seqType = "linear";
 +
sequences.seq_pMLRT_Linearized.comp = true;
 +
sequences.seq_pMLRT_Linearized.data = `LOCUS      pMLRT_Linearized        2133 bp ds-DNA    linear      02-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    terminator      2066..2093
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    terminator      1979..2050
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Scar            1963..1970
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    Scar            2051..2058
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          15..30
 +
                    /label="pMLRT GGA FWD"
 +
                    /note="sequence: ATTACACGTCTCAttggatcccagcaggcc"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(2085..2116)
 +
                    /label="pMLRT GGA REV"
 +
                    /note="sequence: TCGCCTCGTCTCACCCTtctggacatttattatatataaacgcagaaag"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(1367..1387)
 +
                    /label="pMLRT SDM REV"
 +
                    /note="sequence: ggggccagaatgacaagtggc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          1388..1413
 +
                    /label="pMLRT SDM FWD"
 +
                    /note="sequence: ccatgcagtagaggcacttgtcaaac"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 ATTACACGTC TCAttggatc ccagcaggcc tcagggcccg atcgatggga ccaatggggc
 +
      61 agcccctgca agtgttgacc ctaaatatag aagatgagta tcggctacat gagacctcaa
 +
      121 aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
 +
      181 aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
 +
      241 cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
 +
      301 agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
 +
      361 acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
 +
      421 tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
 +
      481 tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
 +
      541 ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
 +
      601 cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
 +
      661 gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
 +
      721 cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
 +
      781 actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
 +
      841 cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
 +
      901 agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
 +
      961 agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
 +
    1021 ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
 +
    1081 attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
 +
    1141 cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
 +
    1201 gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
 +
    1261 tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
 +
    1321 ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca cttgtcattc
 +
    1381 tggcccccca tgcagtagag gcacttgtca aacaaccccc cgaccgctgg ctttccaacg
 +
    1441 cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
 +
    1501 tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
 +
    1561 gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
 +
    1621 cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
 +
    1681 aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
 +
    1741 ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
 +
    1801 gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
 +
    1861 gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
 +
    1921 acgagatctt taaatgacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
 +
    1981 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
 +
    2041 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
 +
    2101 ataataaatg tccagaAGGG TGAGACGAGG CGA
 +
//`
 +
       
 +
sequences.seq_pMLRT_SDM_FWD = {};
 +
sequences.seq_pMLRT_SDM_FWD.varName = "seq_pMLRT_SDM_FWD";
 +
sequences.seq_pMLRT_SDM_FWD.name = "pMLRT SDM FWD";
 +
sequences.seq_pMLRT_SDM_FWD.seqType = "linear";
 +
sequences.seq_pMLRT_SDM_FWD.comp = false;
 +
sequences.seq_pMLRT_SDM_FWD.data = `LOCUS      pMLRT_SDM_FWD            21 bp ds-DNA    linear      08-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 cccatgcagt agaggcactt g
 +
//`
 +
       
 +
sequences.seq_pMLRT_SDM_Product = {};
 +
sequences.seq_pMLRT_SDM_Product.varName = "seq_pMLRT_SDM_Product";
 +
sequences.seq_pMLRT_SDM_Product.name = "pMLRT SDM Product";
 +
sequences.seq_pMLRT_SDM_Product.seqType = "both";
 +
sequences.seq_pMLRT_SDM_Product.comp = true;
 +
sequences.seq_pMLRT_SDM_Product.data = `LOCUS      pMLRT_SDM_Product      5742 bp ds-DNA    circular    07-SEP-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_78234_146112"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    3339..3450
 +
                    /label="on g-block 2"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    terminator      2157..2251
 +
                    /label="lambda t0 terminator"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
    CDS            complement(2282..3076)
 +
                    /label="NeoR/KanR"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    terminator      5486..5513
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    CDS            3405..3419
 +
                    /label="enterokinase site"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    RBS            3339..3350
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    CDS            complement(445..1395)
 +
                    /label="Rep101"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    5537..5652
 +
                    /label="on g-block 3"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    terminator      5399..5470
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    CDS            3363..3398
 +
                    /label="6xHN"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    primer_bind    complement(2393..2412)
 +
                    /label="Neo-F"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    CDS            complement(2282..3073)
 +
                    /label="Kan/neoR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    3357..5357
 +
                    /label="MLRT ORF"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind    3003..3022
 +
                    /label="Neo-R"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    rep_origin      1443..1665
 +
                    /label="pSC101 ori"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind    3255..3273
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    Scar            5471..5478
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    source          1..5742
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    terminator      5566..5652
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    Scar            5383..5390
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          complement(5624..5652)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: atttgtcctactcaggagagcgttcaccg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          4807..4827
 +
                    /label="pMLRT SDM FWD"
 +
                    /note="sequence: cccatgcagtagaggcacttg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          3339..3361
 +
                    /label="pMLRT FWD"
 +
                    /note="sequence: CGTGAGCGTCTCTAAGGaaagaggagaaaggtaccatggg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(5631..5652)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: CTCACGCGTCTCAGCTACTGCAGCGGCCGCTACTAGTAatttgtcctactcaggagagcg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(5631..5652)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: CTCACGCGTCTCAGCTAatttgtcctactcaggagagcg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          5653..5672
 +
                    /label="pMLRT FWD"
 +
                    /note="sequence: TACTAGTAGCGGCCGCTGCAGccgccgccctagacctaggg"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer          3451..3466
 +
                    /label="pMLRT GGA FWD"
 +
                    /note="sequence: ATTACACGTCTCTCcaAggcccgatcgat"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          complement(4787..4806)
 +
                    /label="pMLRT SDM REV"
 +
                    /note="sequence: gggccagaatgacaagtggc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(5505..5536)
 +
                    /label="pMLRT GGA REV"
 +
                    /note="sequence: TCGCCTCGTCTCACCCTtctggacatttattatatataaacgcagaaag"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
ORIGIN
 +
        1 ttcaggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
 +
      61 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
 +
      121 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
 +
      181 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
 +
      241 tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa
 +
      301 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
 +
      361 tttgatatct aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
 +
      421 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
 +
      481 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg
 +
      541 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
 +
      601 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
 +
      661 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
 +
      721 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
 +
      781 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
 +
      841 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
 +
      901 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
 +
      961 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
 +
    1021 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
 +
    1081 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
 +
    1141 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
 +
    1201 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
 +
    1261 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
 +
    1321 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
 +
    1381 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
 +
    1441 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
 +
    1501 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
 +
    1561 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
 +
    1621 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
 +
    1681 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
 +
    1741 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
 +
    1801 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
 +
    1861 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
 +
    1921 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
 +
    1981 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
 +
    2041 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
 +
    2101 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
 +
    2161 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
 +
    2221 gaggtcatta ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg
 +
    2281 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
 +
    2341 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
 +
    2401 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
 +
    2461 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
 +
    2521 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
 +
    2581 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
 +
    2641 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
 +
    2701 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
 +
    2761 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
 +
    2821 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
 +
    2881 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
 +
    2941 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
 +
    3001 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
 +
    3061 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
 +
    3121 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
 +
    3181 ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt attatcatga
 +
    3241 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagttgacag
 +
    3301 ctagctcagt cctaggtact gtgctagcgg aattcattaa agaggagaaa ggtaccatgg
 +
    3361 gtcataatca taatcataat cataatcata atcacaacgg tggagatgac gatgacaagg
 +
    3421 gtggtcgaca agcttggatc cctgcaggcc tcagggcccg atcgatggga ccaatggggc
 +
    3481 agcccctgca agtgttgacc ctaaatatag aagatgagta tcggctacat gagacctcaa
 +
    3541 aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
 +
    3601 aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
 +
    3661 cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
 +
    3721 agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
 +
    3781 acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
 +
    3841 tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
 +
    3901 tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
 +
    3961 ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
 +
    4021 cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
 +
    4081 gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
 +
    4141 cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
 +
    4201 actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
 +
    4261 cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
 +
    4321 agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
 +
    4381 agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
 +
    4441 ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
 +
    4501 attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
 +
    4561 cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
 +
    4621 gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
 +
    4681 tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
 +
    4741 ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca cttgtcattc
 +
    4801 tggcccccca tgcagtagag gcacttgtca aacaaccccc cgaccgctgg ctttccaacg
 +
    4861 cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
 +
    4921 tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
 +
    4981 gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
 +
    5041 cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
 +
    5101 aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
 +
    5161 ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
 +
    5221 gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
 +
    5281 gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
 +
    5341 acgagatctt taaatgacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
 +
    5401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
 +
    5461 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
 +
    5521 ataataaatg tccagacctg caggcatgca agcctctaga ggcatcaaat aaaacgaaag
 +
    5581 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
 +
    5641 agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct
 +
    5701 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gc
 +
//`
 +
       
 +
sequences.seq_pMLRT_SDM_REV = {};
 +
sequences.seq_pMLRT_SDM_REV.varName = "seq_pMLRT_SDM_REV";
 +
sequences.seq_pMLRT_SDM_REV.name = "pMLRT SDM REV";
 +
sequences.seq_pMLRT_SDM_REV.seqType = "linear";
 +
sequences.seq_pMLRT_SDM_REV.comp = false;
 +
sequences.seq_pMLRT_SDM_REV.data = `LOCUS      pMLRT_SDM_REV            20 bp ds-DNA    linear      08-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 gggccagaat gacaagtggc
 +
//`
 +
       
 +
sequences.seq_pMLRT = {};
 +
sequences.seq_pMLRT.varName = "seq_pMLRT";
 +
sequences.seq_pMLRT.name = "pMLRT";
 +
sequences.seq_pMLRT.seqType = "both";
 +
sequences.seq_pMLRT.comp = true;
 +
sequences.seq_pMLRT.data = `LOCUS      pMLRT                  5742 bp ds-DNA    circular    08-JUL-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_78234_146112"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    CDS            complement(2282..3073)
 +
                    /label="Kan/neoR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    terminator      5566..5652
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    CDS            3405..3419
 +
                    /label="enterokinase site"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    terminator      5486..5513
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    CDS            complement(2282..3076)
 +
                    /label="NeoR/KanR"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind    complement(2393..2412)
 +
                    /label="Neo-F"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    terminator      2157..2251
 +
                    /label="lambda t0 terminator"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
    source          1..5742
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    RBS            3339..3350
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    5538..5652
 +
                    /label="on g-block 3"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    rep_origin      1443..1665
 +
                    /label="pSC101 ori"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    CDS            3363..3398
 +
                    /label="6xHN"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    3357..5357
 +
                    /label="MLRT ORF"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    3339..3446
 +
                    /label="on g-block 2"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind    3003..3022
 +
                    /label="Neo-R"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    Scar            5471..5478
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      5399..5470
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    CDS            complement(445..1395)
 +
                    /label="Rep101"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    Scar            5383..5390
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind    3255..3273
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    primer          3339..3361
 +
                    /label="pMLRT FWD"
 +
                    /note="sequence: CGTGAGCGTCTCTAAGGaaagaggagaaaggtaccatggg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer          5653..5672
 +
                    /label="pMLRT FWD"
 +
                    /note="sequence: TACTAGTAGCGGCCGCTGCAGccgccgccctagacctaggg"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer          complement(5631..5652)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: CTCACGCGTCTCAGCTACTGCAGCGGCCGCTACTAGTAatttgtcctactcaggagagcg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(5624..5652)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: atttgtcctactcaggagagcgttcaccg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(5631..5652)
 +
                    /label="pMLRT REV"
 +
                    /note="sequence: CTCACGCGTCTCAGCTAatttgtcctactcaggagagcg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          complement(4787..4806)
 +
                    /label="pMLRT SDM REV"
 +
                    /note="sequence: gggccagaatgacaagtggc"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          4807..4827
 +
                    /label="pMLRT SDM FWD"
 +
                    /note="sequence: cccatgcagtagaggcacttg"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 ttcaggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
 +
      61 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
 +
      121 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
 +
      181 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
 +
      241 tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa
 +
      301 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
 +
      361 tttgatatct aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
 +
      421 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
 +
      481 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg
 +
      541 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
 +
      601 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
 +
      661 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
 +
      721 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
 +
      781 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
 +
      841 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
 +
      901 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
 +
      961 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
 +
    1021 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
 +
    1081 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
 +
    1141 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
 +
    1201 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
 +
    1261 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
 +
    1321 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
 +
    1381 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
 +
    1441 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
 +
    1501 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
 +
    1561 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
 +
    1621 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
 +
    1681 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
 +
    1741 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
 +
    1801 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
 +
    1861 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
 +
    1921 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
 +
    1981 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
 +
    2041 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
 +
    2101 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
 +
    2161 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
 +
    2221 gaggtcatta ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg
 +
    2281 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
 +
    2341 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
 +
    2401 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
 +
    2461 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
 +
    2521 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
 +
    2581 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
 +
    2641 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
 +
    2701 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
 +
    2761 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
 +
    2821 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
 +
    2881 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
 +
    2941 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
 +
    3001 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
 +
    3061 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
 +
    3121 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
 +
    3181 ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt attatcatga
 +
    3241 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagttgacag
 +
    3301 ctagctcagt cctaggtact gtgctagcgg aattcattaa agaggagaaa ggtaccatgg
 +
    3361 gtcataatca taatcataat cataatcata atcacaacgg tggagatgac gatgacaagg
 +
    3421 gtggtcgaca agcttggatc cctgcaggcc tcagggcccg atcgatggga ccaatggggc
 +
    3481 agcccctgca agtgttgacc ctaaatatag aagatgagta tcggctacat gagacctcaa
 +
    3541 aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
 +
    3601 aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
 +
    3661 cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
 +
    3721 agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
 +
    3781 acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
 +
    3841 tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
 +
    3901 tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
 +
    3961 ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
 +
    4021 cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
 +
    4081 gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
 +
    4141 cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
 +
    4201 actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
 +
    4261 cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
 +
    4321 agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
 +
    4381 agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
 +
    4441 ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
 +
    4501 attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
 +
    4561 cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
 +
    4621 gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
 +
    4681 tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
 +
    4741 ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca ctagtcattc
 +
    4801 tggcccccca tgcagtagag gcactagtca aacaaccccc cgaccgctgg ctttccaacg
 +
    4861 cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
 +
    4921 tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
 +
    4981 gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
 +
    5041 cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
 +
    5101 aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
 +
    5161 ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
 +
    5221 gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
 +
    5281 gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
 +
    5341 acgagatctt taaatgacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
 +
    5401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
 +
    5461 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
 +
    5521 ataataaatg tccagacctg caggcatgca agcctctaga ggcatcaaat aaaacgaaag
 +
    5581 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
 +
    5641 agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct
 +
    5701 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gc
 +
//`
 +
       
 +
sequences.seq_pSB1A3GB3_backbone = {};
 +
sequences.seq_pSB1A3GB3_backbone.varName = "seq_pSB1A3GB3_backbone";
 +
sequences.seq_pSB1A3GB3_backbone.name = "pSB1A3-GB3 backbone";
 +
sequences.seq_pSB1A3GB3_backbone.seqType = "both";
 +
sequences.seq_pSB1A3GB3_backbone.comp = true;
 +
sequences.seq_pSB1A3GB3_backbone.data = `LOCUS      pSB1A3-GB3_backbone    5268 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_120731_229341"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    primer_bind    4048..4066
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    promoter        632..662
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    terminator      2004..2061
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind    3638..3657
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    RBS            704..715
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    CDS            complement(3015..3875)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    terminator      1444..1515
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind    complement(2336..2355)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    source          1..5268
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    protein_bind    4452..4485
 +
                    /label="loxP"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    rep_origin      complement(2256..2844)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind    complement(2085..2102)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    promoter        complement(3876..3980)
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    protein_bind    670..686
 +
                    /label="lac operator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    2002..4115
 +
                    /label="pSB1A3 Region"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    CDS            722..1399
 +
                    /label="mRFP1"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    protein_bind    596..617
 +
                    /label="CAP binding site"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    misc_feature    3156..3156
 +
                    /label="Point Mutation"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      1531..1558
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      4070..4113
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer          complement(4088..4115)
 +
                    /label="BBa_Prefix & Promoter SDM Rev"
 +
                    /note="sequence: cagaaatcatccttagcgaaagctaagg"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer          4116..4135
 +
                    /label="BBa_Prefix & Promoter SDM Fwd"
 +
                    /note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          2002..2019
 +
                    /label="pSB1A3 fwd SapI"
 +
                    /note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          complement(4093..4115)
 +
                    /label="BsaI pSB1A3 Rev"
 +
                    /note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          complement(4093..4115)
 +
                    /label="pSB1A3 SapI rev"
 +
                    /note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer          2002..2019
 +
                    /label="BsaI pSB1A3 Fwd"
 +
                    /note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          complement(4093..4115)
 +
                    /label="Linear pSB1A3 BsmBI Rev"
 +
                    /note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          2002..2023
 +
                    /label="Linear pSB1A3 BsmBI Fwd"
 +
                    /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
    primer          2002..2019
 +
                    /label="Linear pSB1A3 BsmBI Fwd"
 +
                    /note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
ORIGIN
 +
        1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
 +
      61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
 +
      121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
 +
      181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
 +
      241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
 +
      301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
 +
      361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
 +
      421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
 +
      481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
 +
      541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
 +
      601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
 +
      661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
 +
      721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
 +
      781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
 +
      841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
 +
      901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
 +
      961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
 +
    1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
 +
    1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
 +
    1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
 +
    1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
 +
    1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
 +
    1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
 +
    1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
 +
    1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
 +
    1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
 +
    1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
 +
    1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
 +
    1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
 +
    1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
 +
    1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
 +
    1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
 +
    1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
 +
    1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
 +
    2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
 +
    2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
 +
    2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
 +
    2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
 +
    2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
 +
    2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
 +
    2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
 +
    2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
 +
    2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
 +
    2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
 +
    2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
 +
    2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
 +
    2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
 +
    2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
 +
    2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
 +
    2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
 +
    3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
 +
    3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
 +
    3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
 +
    3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
 +
    3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
 +
    3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
 +
    3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
 +
    3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
 +
    3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
 +
    3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
 +
    3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
 +
    3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
 +
    3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
 +
    3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
 +
    3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
 +
    3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
 +
    3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
 +
    4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
 +
    4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
 +
    4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
 +
    4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
 +
    4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
 +
    4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
 +
    4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
 +
    4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
 +
    4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
 +
    4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
 +
    4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
 +
    4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
 +
    4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
 +
    4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
 +
    4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
 +
    4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
 +
    4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
 +
    5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
 +
    5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
 +
    5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
 +
    5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
 +
//`
 +
       
 +
sequences.seq_pSB1C3_Forward_Primer = {};
 +
sequences.seq_pSB1C3_Forward_Primer.varName = "seq_pSB1C3_Forward_Primer";
 +
sequences.seq_pSB1C3_Forward_Primer.name = "pSB1C3 Forward Primer";
 +
sequences.seq_pSB1C3_Forward_Primer.seqType = "linear";
 +
sequences.seq_pSB1C3_Forward_Primer.comp = false;
 +
sequences.seq_pSB1C3_Forward_Primer.data = `LOCUS      pSB1C3_Forward_Primer    41 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TATGATCGTC TCAcaaagtc agaacagatg tttgccgatg c
 +
//`
 +
       
 +
sequences.seq_pSB1C3_Reverse_Primer = {};
 +
sequences.seq_pSB1C3_Reverse_Primer.varName = "seq_pSB1C3_Reverse_Primer";
 +
sequences.seq_pSB1C3_Reverse_Primer.name = "pSB1C3 Reverse Primer";
 +
sequences.seq_pSB1C3_Reverse_Primer.seqType = "linear";
 +
sequences.seq_pSB1C3_Reverse_Primer.comp = false;
 +
sequences.seq_pSB1C3_Reverse_Primer.data = `LOCUS      pSB1C3_Reverse_Primer    45 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ACTGATCGTC TCTgttgtga agaaaaagtg aatgatgtag ccgtc
 +
//`
 +
       
 +
sequences.seq_pSB1K3_containing_mRFP1 = {};
 +
sequences.seq_pSB1K3_containing_mRFP1.varName = "seq_pSB1K3_containing_mRFP1";
 +
sequences.seq_pSB1K3_containing_mRFP1.name = "pSB1K3 containing mRFP1";
 +
sequences.seq_pSB1K3_containing_mRFP1.seqType = "both";
 +
sequences.seq_pSB1K3_containing_mRFP1.comp = true;
 +
sequences.seq_pSB1K3_containing_mRFP1.data = `LOCUS      pSB1K3_containing_mRFP1 3273 bp ds-DNA    circular    07-JUL-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_118082_252166"                                 
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    protein_bind    53..69
 +
                    /label="lac operator"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    rep_origin      complement(1221..1809)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind    complement(1050..1067)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    RBS            87..98
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer_bind    2849..2868
 +
                    /label="pENTR-R"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      827..898
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    primer_bind    2765..2784
 +
                    /label="Kan-R"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    terminator      3084..3127
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    primer_bind    complement(1301..1320)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    source          1..3273
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    CDS            complement(2042..2857)
 +
                    /label="KanR"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind    3062..3080
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    misc_feature    3130..3151
 +
                    /label="BioBrick prefix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    promoter        15..45
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    CDS            105..782
 +
                    /label="mRFP1"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    protein_bind    3252..3273
 +
                    /label="CAP binding site"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    948..968
 +
                    /label="BioBrick suffix"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    terminator      969..1026
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    terminator      914..941
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
ORIGIN
 +
        1 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
 +
      61 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatggct tcctccgaag
 +
      121 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
 +
      181 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
 +
      241 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
 +
      301 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
 +
      361 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
 +
      421 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
 +
      481 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
 +
      541 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
 +
      601 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
 +
      661 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
 +
      721 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
 +
      781 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
 +
      841 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
 +
      901 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagtagcggc
 +
      961 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
 +
    1021 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
 +
    1081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
 +
    1141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
 +
    1201 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
 +
    1261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
 +
    1321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
 +
    1381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
 +
    1441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
 +
    1501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
 +
    1561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
 +
    1621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
 +
    1681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
 +
    1741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
 +
    1801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
 +
    1861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
 +
    1921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
 +
    1981 tcgagtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg
 +
    2041 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
 +
    2101 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
 +
    2161 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
 +
    2221 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
 +
    2281 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
 +
    2341 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
 +
    2401 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
 +
    2461 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
 +
    2521 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
 +
    2581 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
 +
    2641 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
 +
    2701 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
 +
    2761 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
 +
    2821 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
 +
    2881 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
 +
    2941 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
 +
    3001 atcagctcga gtgccacttg acgtctaaga aaccattatt atcatgacat taacctataa
 +
    3061 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
 +
    3121 tgatttctgg aattcgcggc cgcttctaga gcaatacgca aaccgcctct ccccgcgcgt
 +
    3181 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
 +
    3241 cgcaacgcaa ttaatgtgag ttagctcact cat
 +
//`
 +
       
 +
sequences.seq_pSB1T3_Backbone = {};
 +
sequences.seq_pSB1T3_Backbone.varName = "seq_pSB1T3_Backbone";
 +
sequences.seq_pSB1T3_Backbone.name = "pSB1T3 Backbone";
 +
sequences.seq_pSB1T3_Backbone.seqType = "both";
 +
sequences.seq_pSB1T3_Backbone.comp = true;
 +
sequences.seq_pSB1T3_Backbone.data = `LOCUS      pSB1T3_Backbone        2498 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    primer_bind    2389..2407
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer_bind    complement(120..137)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    18..38
 +
                    /label="BioBrick suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    2456..2477
 +
                    /label="BioBrick prefix"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    terminator      39..96
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    terminator      2411..2454
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind    complement(371..390)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    rep_origin      complement(291..879)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    CDS            1145..2329
 +
                    /label="TetR"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
ORIGIN
 +
        1 GGACTAGGTC TCATGCCtac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg
 +
      61 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
 +
      121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
 +
      181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
 +
      241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
 +
      301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
 +
      361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
 +
      421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
 +
      481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
 +
      541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
 +
      601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
 +
      661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
 +
      721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
 +
      781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
 +
      841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
 +
      901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
 +
      961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
 +
    1021 agtatatatg agtaaacttg gtctgacagc tcgagattct catgtttgac agcttatcat
 +
    1081 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
 +
    1141 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
 +
    1201 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
 +
    1261 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
 +
    1321 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
 +
    1381 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
 +
    1441 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
 +
    1501 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
 +
    1561 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
 +
    1621 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
 +
    1681 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
 +
    1741 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
 +
    1801 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
 +
    1861 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
 +
    1921 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
 +
    1981 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
 +
    2041 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
 +
    2101 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
 +
    2161 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
 +
    2221 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
 +
    2281 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aactcgagtg
 +
    2341 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
 +
    2401 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctgaatt
 +
    2461 cgcggccgct tctagaggCG TTAGAGACCT AGAGCATT
 +
//`
 +
       
 +
sequences.seq_pUCSTS_FWD_BspMI = {};
 +
sequences.seq_pUCSTS_FWD_BspMI.varName = "seq_pUCSTS_FWD_BspMI";
 +
sequences.seq_pUCSTS_FWD_BspMI.name = "pUC-STS FWD BspMI";
 +
sequences.seq_pUCSTS_FWD_BspMI.seqType = "linear";
 +
sequences.seq_pUCSTS_FWD_BspMI.comp = false;
 +
sequences.seq_pUCSTS_FWD_BspMI.data = `LOCUS      pUC-STS_FWD_BspMI        35 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ACAGTGGGTC TCCTAGTatg gtgtctgtga gtgag
 +
//`
 +
       
 +
sequences.seq_pUCSTS_REV_BspMI = {};
 +
sequences.seq_pUCSTS_REV_BspMI.varName = "seq_pUCSTS_REV_BspMI";
 +
sequences.seq_pUCSTS_REV_BspMI.name = "pUC-STS REV BspMI";
 +
sequences.seq_pUCSTS_REV_BspMI.seqType = "linear";
 +
sequences.seq_pUCSTS_REV_BspMI.comp = false;
 +
sequences.seq_pUCSTS_REV_BspMI.data = `LOCUS      pUC-STS_REV_BspMI        35 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ACAGTCGGTC TCCacaatta tatggccatg ctgcg
 +
//`
 +
       
 +
sequences.seq_pUCSTS = {};
 +
sequences.seq_pUCSTS.varName = "seq_pUCSTS";
 +
sequences.seq_pUCSTS.name = "pUC-STS";
 +
sequences.seq_pUCSTS.seqType = "both";
 +
sequences.seq_pUCSTS.comp = true;
 +
sequences.seq_pUCSTS.data = `LOCUS      pUC-STS                3622 bp ds-DNA    circular    25-OCT-2020
 +
DEFINITION  .
 +
KEYWORDS    "accession:addgene_35949_44214"                                   
 +
SOURCE      synthetic DNA construct                                           
 +
  ORGANISM  synthetic DNA construct                                           
 +
FEATURES            Location/Qualifiers
 +
    CDS            1632..2492
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    1154..1156
 +
                    /label="Threonine inside BsaI site"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    primer_bind    complement(1262..1281)
 +
                    /label="pRS-marker"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind    3152..3171
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    source          23..1192
 +
                    /label="STS"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    rep_origin      2663..3251
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    promoter        1527..1631
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    promoter        3575..3605
 +
                    /label="lac promoter"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind    complement(1850..1869)
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    primer_bind    complement(1441..1459)
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    protein_bind    3539..3560
 +
                    /label="CAP binding site"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    misc_feature    794..796
 +
                    /label="Leu Codon inside BsaI Site "
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    primer_bind    1381..1403
 +
                    /label="pGEX 3'"
 +
                    /ApEinfo_revcolor=#d59687
 +
                    /ApEinfo_fwdcolor=#d59687
 +
    primer_bind    3405..3422
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          complement(1173..1192)
 +
                    /label="STS Reverse BspMI"
 +
                    /note="sequence: ACTCCTACCTGCTTCaACAAttatatggccatgctgcgga"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer          complement(1193..1210)
 +
                    /label="stsreverseprimer"
 +
                    /note="sequence: aaataccgcagcggccgc"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    primer          23..44
 +
                    /label="STS Forward BspMI"
 +
                    /note="sequence: ACTGCTACCTGCTCCcTAGTatggtgtctgtgagtgagatcc"
 +
                    /ApEinfo_revcolor=#d6b295
 +
                    /ApEinfo_fwdcolor=#d6b295
 +
    primer          1..22
 +
                    /label="forward primer"
 +
                    /note="sequence: ctagaagaaggaggattacaaa"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 ctagaagaag gaggattaca aaatggtgtc tgtgagtgag atccgcaaag ttcaaagggc
 +
      61 agaaggccct gcaactgtat tggcgatagg cacagcaaat ccaccaaatt gtattgatca
 +
      121 gagcacatat gctgattatt attttagagt aactaacagt gaacacatga ctgatctcaa
 +
      181 gaagaagttt cagcgcattt gtgagagaac acaaatcaag aacagacata tgtacttaac
 +
      241 agaagagata ctgaaagaga atcctaacat gtgcgcatac aaagcaccgt cgttggatgc
 +
      301 aagggaagac atgatgatca gggaggtacc aagggttgga aaagaggctg caaccaaggc
 +
      361 catcaaggaa tggggtcagc caatgtctaa gatcacacat ttgatcttct gcaccaccag
 +
      421 cggtgttgca ttgcctggcg ttgattacga actcatcgta ctcttaggac tcgacccatc
 +
      481 cgtcaagagg tacatgatgt accaccaagg ctgcttcgcc ggtggcactg tccttcgttt
 +
      541 ggctaaggac ttggctgaaa acaacaagga tgctcgtgtg cttatcgttt gttctgagaa
 +
      601 taccgcagtc actttccgtg gtcctagtga gacagacatg gatagtcttg tagggcaagc
 +
      661 cttgtttgct gatggagctg ctgcgattat cattggttct gatcctgtgc cagaggttga
 +
      721 aaagcctatc tttgaaattg tttcgactga tcaaaaactt gtccctaaca gccatggagc
 +
      781 catcggtggt ctccttcgtg aagttgggct tacattctat cttaataaga gtgttcctga
 +
      841 tattatttca caaaacatca atgatgcgct cagtaaagct tttgatccat tgggtatatc
 +
      901 tgattataac tcaatatttt ggattgcaca ccctggtgga cgtgcaattt tggatcaggt
 +
      961 tgaacagaaa gtgaacttga aaccagagaa gatgaatgcc actagagacg tgcttagcaa
 +
    1021 ttacggtaac atgtcaagtg cgtgtgtgtt cttcatcatg gatttaatga ggaagaagtc
 +
    1081 ccttgaagaa ggacttaaaa ctaccggtga aggacttgat tggggcgtac tttttggctt
 +
    1141 tggtcctggt ctcactattg aaactgttgt tctccgcagc atggccatat aagcggccgc
 +
    1201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
 +
    1261 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
 +
    1321 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
 +
    1381 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
 +
    1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
 +
    1501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
 +
    1561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
 +
    1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
 +
    1681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
 +
    1741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
 +
    1801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
 +
    1861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
 +
    1921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
 +
    1981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
 +
    2041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
 +
    2101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
 +
    2161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
 +
    2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
 +
    2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
 +
    2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
 +
    2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
 +
    2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
 +
    2521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
 +
    2581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
 +
    2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
 +
    2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
 +
    2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
 +
    2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
 +
    2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
 +
    2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
 +
    3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
 +
    3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
 +
    3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
 +
    3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
 +
    3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
 +
    3301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
 +
    3361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
 +
    3421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
 +
    3481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
 +
    3541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
 +
    3601 tgttgtgtgg aattgtgagc gt
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_Assembly = {};
 +
sequences.seq_Resveratrol_2x_Assembly.varName = "seq_Resveratrol_2x_Assembly";
 +
sequences.seq_Resveratrol_2x_Assembly.name = "Resveratrol 2x Assembly";
 +
sequences.seq_Resveratrol_2x_Assembly.seqType = "both";
 +
sequences.seq_Resveratrol_2x_Assembly.comp = true;
 +
sequences.seq_Resveratrol_2x_Assembly.data = `LOCUS      Resveratrol_2x_Assembly 2448 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2436..2444
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2425..2433
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2414..2422
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    HIV-RT Binding Site join(2445..2448,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2403..2411
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_Comp_Assembly = {};
 +
sequences.seq_Resveratrol_2x_Comp_Assembly.varName = "seq_Resveratrol_2x_Comp_Assembly";
 +
sequences.seq_Resveratrol_2x_Comp_Assembly.name = "Resveratrol 2x Comp Assembly";
 +
sequences.seq_Resveratrol_2x_Comp_Assembly.seqType = "both";
 +
sequences.seq_Resveratrol_2x_Comp_Assembly.comp = true;
 +
sequences.seq_Resveratrol_2x_Comp_Assembly.data = `LOCUS      Resveratrol_2x_Comp_Ass 2448 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    HIV-RT Binding Site join(2445..2448,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2436..2444)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2425..2433)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    complement(2414..2422)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    complement(2403..2411)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2392..2400)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
 +
    2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_Full_Part = {};
 +
sequences.seq_Resveratrol_2x_Full_Part.varName = "seq_Resveratrol_2x_Full_Part";
 +
sequences.seq_Resveratrol_2x_Full_Part.name = "Resveratrol 2x Full Part";
 +
sequences.seq_Resveratrol_2x_Full_Part.seqType = "both";
 +
sequences.seq_Resveratrol_2x_Full_Part.comp = true;
 +
sequences.seq_Resveratrol_2x_Full_Part.data = `LOCUS      Resveratrol_2x_Full_Par 2747 bp ds-DNA    circular    14-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    2414..2422
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Promoter            2632..2666
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site 2445..2554
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    HIV-RT Binding Site join(2744..2747,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2735..2743)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    misc_binding        2668..2690
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2713..2721)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Scar                2624..2631
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Terminator          2555..2623
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2425..2433
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2436..2444
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    2403..2411
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site    complement(2724..2732)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2702..2710)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2691..2699)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    gBlock              2501..2625
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
 +
    2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
 +
    2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
 +
    2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
 +
    2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
 +
    2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex = {};
 +
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Complement_Duplex";
 +
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.name = "Resveratrol 2x r_oligo Complement Duplex";
 +
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.seqType = "linear";
 +
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.comp = true;
 +
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.data = `LOCUS      Resveratrol_2x_r_oligo_C 111 bp ds-DNA    linear      25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    complement(42..50)
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    complement(53..61)
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    complement(64..72)
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    complement(75..83)
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site complement(86..94)
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
 +
      61 CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAACG AGACCAACCT G
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1 = {};
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.name = "Resveratrol 2x r_oligo Duplex 11-33 FWD 1";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.seqType = "linear";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.comp = false;
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.data = `LOCUS      Resveratrol_2x_r_oligo_Du 23 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCCTAGCTGG AATTGTGAGC GGA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1 = {};
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.name = "Resveratrol 2x r_oligo Duplex 75-94 REV 1";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.seqType = "linear";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.comp = false;
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.data = `LOCUS      Resveratrol_2x_r_oligo_Du 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TTTCCACACA TCGCCCACGC
 +
//`
 +
       
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex = {};
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Duplex";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex.name = "Resveratrol 2x r_oligo Duplex";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex.seqType = "linear";
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex.comp = true;
 +
sequences.seq_Resveratrol_2x_r_oligo_Duplex.data = `LOCUS      Resveratrol_2x_r_oligo_D 111 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site 42..50
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    misc_feature    53..61
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    64..72
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    75..83
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    86..94
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          complement(75..94)
 +
                    /label="Resveratrol 2x r_oligo Duplex 75-94 REV 1"
 +
                    /note="sequence: TTTCCACACATCGCCCACGC"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          11..33
 +
                    /label="Resveratrol 2x r_oligo Duplex 11-33 FWD 1"
 +
                    /note="sequence: TCCTAGCTGGAATTGTGAGCGGA"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
 +
      61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAACG AGACCAACCT G
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_Assembly = {};
 +
sequences.seq_Resveratrol_4x_Assembly.varName = "seq_Resveratrol_4x_Assembly";
 +
sequences.seq_Resveratrol_4x_Assembly.name = "Resveratrol 4x Assembly";
 +
sequences.seq_Resveratrol_4x_Assembly.seqType = "both";
 +
sequences.seq_Resveratrol_4x_Assembly.comp = true;
 +
sequences.seq_Resveratrol_4x_Assembly.data = `LOCUS      Resveratrol_4x_Assembly 2492 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    2447..2455
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2469..2477
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2458..2466
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Zinc Finger Site    2403..2411
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    2425..2433
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Zinc Finger Site    2436..2444
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2414..2422
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    HIV-RT Binding Site join(2489..2492,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2480..2488
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
 +
    2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_Comp_Assembly = {};
 +
sequences.seq_Resveratrol_4x_Comp_Assembly.varName = "seq_Resveratrol_4x_Comp_Assembly";
 +
sequences.seq_Resveratrol_4x_Comp_Assembly.name = "Resveratrol 4x Comp Assembly";
 +
sequences.seq_Resveratrol_4x_Comp_Assembly.seqType = "both";
 +
sequences.seq_Resveratrol_4x_Comp_Assembly.comp = true;
 +
sequences.seq_Resveratrol_4x_Comp_Assembly.data = `LOCUS      Resveratrol_4x_Comp_Ass 2492 bp ds-DNA    circular    25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Zinc Finger Site    complement(2469..2477)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2392..2400)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    complement(2458..2466)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    complement(2480..2488)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2414..2422)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    complement(2436..2444)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    HIV-RT Binding Site join(2489..2492,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2425..2433)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site    complement(2403..2411)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    complement(2447..2455)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
 +
    2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT
 +
    2461 CCACACATCG CCCACGCATG CCGTCCTCAA AA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_Full_Part = {};
 +
sequences.seq_Resveratrol_4x_Full_Part.varName = "seq_Resveratrol_4x_Full_Part";
 +
sequences.seq_Resveratrol_4x_Full_Part.name = "Resveratrol 4x Full Part";
 +
sequences.seq_Resveratrol_4x_Full_Part.seqType = "both";
 +
sequences.seq_Resveratrol_4x_Full_Part.comp = true;
 +
sequences.seq_Resveratrol_4x_Full_Part.data = `LOCUS      Resveratrol_4x_Full_Par 2835 bp ds-DNA    circular    14-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    Zinc Finger Site    complement(2823..2831)
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site    complement(2768..2776)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2392..2400
 +
                        /label="ZFc Site"
 +
                        /ApEinfo_revcolor=#b7e6d7
 +
                        /ApEinfo_fwdcolor=#b7e6d7
 +
    Zinc Finger Site    2403..2411
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2480..2488
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    terminator          2265..2308
 +
                        /label="bacterial terminator"
 +
                        /ApEinfo_revcolor=#ffef86
 +
                        /ApEinfo_fwdcolor=#ffef86
 +
    Scar                2668..2675
 +
                        /label="BioBrick Scar"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind        complement(280..297)
 +
                        /label="L4440"
 +
                        /ApEinfo_revcolor=#75c6a9
 +
                        /ApEinfo_fwdcolor=#75c6a9
 +
    Zinc Finger Site    complement(2812..2820)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        2243..2261
 +
                        /label="pBRforEco"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    2414..2422
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature        1351..1351
 +
                        /label="Point Mutation"
 +
                        /ApEinfo_revcolor=#9eafd2
 +
                        /ApEinfo_fwdcolor=#9eafd2
 +
    Promoter            2333..2367
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2779..2787)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    2436..2444
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    primer_bind        complement(531..550)
 +
                        /label="pBR322ori-F"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    Biobricking        2311..2332
 +
                        /label="BioBrick Prefix "
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    HIV-RT Binding Site 2489..2598
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    2458..2466
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    HIV-RT Binding Site join(2832..2835,1..106)
 +
                        /label="C* HTBS"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    Zinc Finger Site    complement(2735..2743)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_binding        2712..2734
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Terminator          2599..2667
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2425..2433
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site    2469..2477
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Backbone            197..2310
 +
                        /label="Mutated pSB1A3"
 +
                        /ApEinfo_revcolor=#f58a5e
 +
                        /ApEinfo_fwdcolor=#f58a5e
 +
    Promoter            2676..2710
 +
                        /label="J23100 Anderson Promoter"
 +
                        /ApEinfo_revcolor=#d59687
 +
                        /ApEinfo_fwdcolor=#d59687
 +
    Zinc Finger Site    complement(2790..2798)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    gBlock              2545..2669
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    promoter            complement(2071..2175)
 +
                        /label="AmpR promoter"
 +
                        /ApEinfo_revcolor=#faac61
 +
                        /ApEinfo_fwdcolor=#faac61
 +
    CDS                complement(1210..2070)
 +
                        /label="AmpR"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2757..2765)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    Zinc Finger Site    2447..2455
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    primer_bind        1833..1852
 +
                        /label="Amp-R"
 +
                        /ApEinfo_revcolor=#b4abac
 +
                        /ApEinfo_fwdcolor=#b4abac
 +
    misc_binding        2369..2391
 +
                        /label="LacO"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    gBlock              53..196
 +
                        /label="gBlock B"
 +
                        /ApEinfo_revcolor=#85dae9
 +
                        /ApEinfo_fwdcolor=#85dae9
 +
    Zinc Finger Site    complement(2801..2809)
 +
                        /label="PBSII Site"
 +
                        /ApEinfo_revcolor=#c7b0e3
 +
                        /ApEinfo_fwdcolor=#c7b0e3
 +
    rep_origin          complement(451..1039)
 +
                        /label="ori"
 +
                        /ApEinfo_revcolor=#b1ff67
 +
                        /ApEinfo_fwdcolor=#b1ff67
 +
    Zinc Finger Site    complement(2746..2754)
 +
                        /label="Zif268 Site"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
    Biobricking        176..196
 +
                        /label="BioBrick Suffix"
 +
                        /ApEinfo_revcolor=#84b0dc
 +
                        /ApEinfo_fwdcolor=#84b0dc
 +
    Terminator          107..175
 +
                        /label="B0054"
 +
                        /ApEinfo_revcolor=#c6c9d1
 +
                        /ApEinfo_fwdcolor=#c6c9d1
 +
ORIGIN
 +
        1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
 +
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
 +
      121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
 +
      181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
 +
      241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
 +
      301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
 +
      361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
 +
      421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
 +
      481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
 +
      541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
 +
      601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
 +
      661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
 +
      721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
 +
      781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
 +
      841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
 +
      901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
 +
      961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
 +
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
 +
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
 +
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
 +
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
 +
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
 +
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
 +
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
 +
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
 +
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
 +
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
 +
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
 +
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
 +
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
 +
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
 +
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
 +
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
 +
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
 +
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
 +
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
 +
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
 +
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
 +
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
 +
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
 +
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
 +
    2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
 +
    2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
 +
    2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
 +
    2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
 +
    2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
 +
    2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
 +
    2821 ATGCCGTCCT CAAAA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex = {};
 +
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Complement_Duplex";
 +
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.name = "Resveratrol 4x r_oligo Complement Duplex";
 +
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.seqType = "linear";
 +
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.comp = true;
 +
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.data = `LOCUS      Resveratrol_4x_r_oligo_C 155 bp ds-DNA    linear      25-JUN-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    complement(42..50)
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    complement(53..61)
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    complement(64..72)
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    complement(75..83)
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    complement(86..94)
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    complement(97..105)
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    complement(108..116)
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    complement(119..127)
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    Zinc Finger Site complement(130..138)
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
 +
      61 CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT CCACACATCG
 +
      121 CCCACGCATG CCGTCCTCAA AACGAGACCA ACCTG
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1 = {};
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.name = "Resveratrol 4x r_oligo Duplex 109-128 REV 1";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.seqType = "linear";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.comp = false;
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.data = `LOCUS      Resveratrol_4x_r_oligo_Du 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 TCGCCCACGC ATTTTCCACA
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1 = {};
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.name = "Resveratrol 4x r_oligo Duplex 39-58 FWD 1";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.seqType = "linear";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.comp = false;
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.data = `LOCUS      Resveratrol_4x_r_oligo_Du 20 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 ATTGAGGACG GCATGCGTGG
 +
//`
 +
       
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex = {};
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Duplex";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex.name = "Resveratrol 4x r_oligo Duplex";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex.seqType = "linear";
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex.comp = true;
 +
sequences.seq_Resveratrol_4x_r_oligo_Duplex.data = `LOCUS      Resveratrol_4x_r_oligo_D 155 bp ds-DNA    linear      07-JUL-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    19..41
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Zinc Finger Site 42..50
 +
                    /label="ZFc Site"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    misc_feature    53..61
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    64..72
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    75..83
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    86..94
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    97..105
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    108..116
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    119..127
 +
                    /label="Zif268"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    130..138
 +
                    /label="PBSII Site"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          39..58
 +
                    /label="Resveratrol 4x r_oligo Duplex 39-58 FWD 1"
 +
                    /note="sequence: ATTGAGGACGGCATGCGTGG"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    primer          complement(109..128)
 +
                    /label="Resveratrol 4x r_oligo Duplex 109-128 REV 1"
 +
                    /note="sequence: TCGCCCACGCATTTTCCACA"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
ORIGIN
 +
        1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
 +
      61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG TGGAAAATGC
 +
      121 GTGGGCGATG TGTGGAAAAA AACGAGACCA ACCTG
 +
//`
 +
       
 +
sequences.seq_r_oligo_Flipper = {};
 +
sequences.seq_r_oligo_Flipper.varName = "seq_r_oligo_Flipper";
 +
sequences.seq_r_oligo_Flipper.name = "r_oligo Flipper";
 +
sequences.seq_r_oligo_Flipper.seqType = "both";
 +
sequences.seq_r_oligo_Flipper.comp = true;
 +
sequences.seq_r_oligo_Flipper.data = `LOCUS      r_oligo_Flipper        3220 bp ds-DNA    circular    02-SEP-2020
 
DEFINITION  .
 
DEFINITION  .
 
FEATURES            Location/Qualifiers
 
FEATURES            Location/Qualifiers
Line 282: Line 7,494:
 
     3121 TTTTTTatta gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt
 
     3121 TTTTTTatta gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt
 
     3181 ggggttatca ttgggtacta gtagcggccg ctgcagagtc
 
     3181 ggggttatca ttgggtacta gtagcggccg ctgcagagtc
 +
//`
 +
       
 +
sequences.seq_SapI_FWD_Primer = {};
 +
sequences.seq_SapI_FWD_Primer.varName = "seq_SapI_FWD_Primer";
 +
sequences.seq_SapI_FWD_Primer.name = "SapI FWD Primer";
 +
sequences.seq_SapI_FWD_Primer.seqType = "linear";
 +
sequences.seq_SapI_FWD_Primer.comp = false;
 +
sequences.seq_SapI_FWD_Primer.data = `LOCUS      SapI_FWD_Primer          32 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 AAAGCTCTTC AGGAagtccg gcaaaaaagg gc
 +
//`
 +
       
 +
sequences.seq_SapI_Rev_Primer = {};
 +
sequences.seq_SapI_Rev_Primer.varName = "seq_SapI_Rev_Primer";
 +
sequences.seq_SapI_Rev_Primer.name = "SapI Rev Primer";
 +
sequences.seq_SapI_Rev_Primer.seqType = "linear";
 +
sequences.seq_SapI_Rev_Primer.comp = false;
 +
sequences.seq_SapI_Rev_Primer.data = `LOCUS      SapI_Rev_Primer          37 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
ORIGIN
 +
        1 CCCGCTCTTC AGTGcagaaa tcatccttag cgaaagc
 +
//`
 +
       
 +
sequences.seq_STS_intro_gblock = {};
 +
sequences.seq_STS_intro_gblock.varName = "seq_STS_intro_gblock";
 +
sequences.seq_STS_intro_gblock.name = "STS intro gblock";
 +
sequences.seq_STS_intro_gblock.seqType = "linear";
 +
sequences.seq_STS_intro_gblock.comp = true;
 +
sequences.seq_STS_intro_gblock.data = `LOCUS      STS_intro_gblock        474 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    40..74
 +
                    /label="BBa_J23111"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_feature    123..431
 +
                    /label="PBSII"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    misc_feature    99..112
 +
                    /label="BBa_J61101"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    Biobricking    18..39
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_binding    75..98
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    432..461
 +
                    /label="Spacer"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          18..41
 +
                    /label="forward Prefix-Promoter-PBS2-sapI primer"
 +
                    /note="sequence: AAAGCTCTTCACACgaattcgcggccgcttctagagtt"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer          complement(439..461)
 +
                    /label="reverse Prefix-Promoter-PBS2-sapI primer"
 +
                    /note="sequence: AAAGCTCTTCAACTactacctccacttccacctccac"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
ORIGIN
 +
        1 ACCATCGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
 +
      61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGACAGGAC CCtaaataca
 +
      121 tgATGCATCA CCATCACCAT CATCCAGGCG AGAAACCGTA CGCCTGCCCC GAATGCGGTA
 +
      181 AATCATTTAG TCAGCGGGCG AACTTACGCG CACACCAGCG AACCCATACC GGAGAAAAAC
 +
      241 CGTATAAATG TCCGGAATGT GGTAAAAGCT TTAGCCGTTC GGATCATCTG ACGACTCACC
 +
      301 AACGCACACA CACCGGGGAA AAGCCGTATA AATGCCCTGA GTGTGGTAAG TCGTTCTCCC
 +
      361 GTTCTGACGT TCTGGTGCGC CATCAGCGTA CGCATACTGG TGGCGGGAGC GGCGGCGGAA
 +
      421 GTGGTGGCTC Cggtggaagt ggaggtggaa gtggaggtag tAGAGACCTC CTTT
 +
//`
 +
       
 +
sequences.seq_STS_outro_gblock = {};
 +
sequences.seq_STS_outro_gblock.varName = "seq_STS_outro_gblock";
 +
sequences.seq_STS_outro_gblock.name = "STS outro gblock";
 +
sequences.seq_STS_outro_gblock.seqType = "linear";
 +
sequences.seq_STS_outro_gblock.comp = true;
 +
sequences.seq_STS_outro_gblock.data = `LOCUS      STS_outro_gblock        129 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    stem_loop      27..78
 +
                    /label="T0 terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    Biobricking    79..99
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 gtcaagctgc catgacgagG GTCTCAttgt tcagaacgct cggtcttgca caccgggcgt
 +
      61 tttttctttg tgagtccata ctagtagcgg ccgctgcagT GCCAGAGACC tccaagagag
 +
      121 tgaagggat
 +
//`
 +
       
 +
sequences.seq_ultramer_duplex_2 = {};
 +
sequences.seq_ultramer_duplex_2.varName = "seq_ultramer_duplex_2";
 +
sequences.seq_ultramer_duplex_2.name = "ultramer duplex 2";
 +
sequences.seq_ultramer_duplex_2.seqType = "linear";
 +
sequences.seq_ultramer_duplex_2.comp = true;
 +
sequences.seq_ultramer_duplex_2.data = `LOCUS      ultramer_duplex_2        215 bp ds-DNA    linear      06-SEP-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_binding    54..76
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    RBS            87..98
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    CDS            111..146
 +
                    /label="6xHN"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    18..52
 +
                    /label="'BBa_J23109'"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    CDS            153..167
 +
                    /label="enterokinase site"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 TTATCCCGTC TCATGCGttt acagctagct cagtcctagg gactgtgcta gcTGGAATTG
 +
      61 TGAGCGGATA ACAATTcgtt gcaactaaag aggagaaagg taccatgggt cataatcata
 +
      121 atcataatca taatcataat cacaacggtg gagatgacga tgacaagggt ggtcgacaag
 +
      181 cttggatccc agcaggccTT GGTGAGACGG TGATT
 +
//`
 +
       
 +
sequences.seq_Updated_Assembly_in_Amp = {};
 +
sequences.seq_Updated_Assembly_in_Amp.varName = "seq_Updated_Assembly_in_Amp";
 +
sequences.seq_Updated_Assembly_in_Amp.name = "Updated Assembly in Amp";
 +
sequences.seq_Updated_Assembly_in_Amp.seqType = "both";
 +
sequences.seq_Updated_Assembly_in_Amp.comp = true;
 +
sequences.seq_Updated_Assembly_in_Amp.data = `LOCUS      Updated_Assembly_in_Amp 7888 bp ds-DNA    circular    24-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    None            5477..5511
 +
                    /label="'BBa_J23109'"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    Scar            5415..5422
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      2065..2108
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    1151..1151
 +
                    /label="Point Mutation"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    Scar            7678..7685
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      7777..7863
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    misc_binding    5513..5535
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    primer_bind    1633..1652
 +
                    /label="Amp-R"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    misc_feature    2283..3968
 +
                    /label="HIVRT p66"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    primer_bind    2043..2061
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    CDS            5570..5605
 +
                    /label="6xHN"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_binding    2169..2191
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    RBS            3977..3988
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    CDS            complement(1010..1870)
 +
                    /label="AmpR"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    Biobricking    7864..7884
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Biobricking    2111..2132
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    RBS            5546..5557
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    RBS            2265..2276
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    terminator      7606..7677
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature    3995..5320
 +
                    /label="HIVRT P51"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    terminator      7693..7720
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    rep_origin      complement(251..839)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    Scar            7590..7597
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    2133..2167
 +
                    /label="BBa_J23109'"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    5564..7564
 +
                    /label="MLRT ORF"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_feature    2207..2256
 +
                    /label="sTRSV HHRz"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    primer_bind    complement(331..350)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    primer_bind    complement(80..97)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    terminator      5343..5414
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    terminator      5430..5457
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    promoter        complement(1871..1975)
 +
                    /label="AmpR promoter"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    Scar            5327..5334
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    misc_feature    5612..5626
 +
                    /label="Enterokinase Site"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
 +
      61 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
 +
      121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
 +
      181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
 +
      241 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
 +
      301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
 +
      361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
 +
      421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
 +
      481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
 +
      541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
 +
      601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
 +
      661 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
 +
      721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
 +
      781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
 +
      841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
 +
      901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
 +
      961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
 +
    1021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
 +
    1081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
 +
    1141 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
 +
    1201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
 +
    1261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
 +
    1321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
 +
    1381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
 +
    1441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
 +
    1501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
 +
    1561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
 +
    1621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
 +
    1681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatat
 +
    1741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
 +
    1801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
 +
    1861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
 +
    1921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
 +
    1981 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
 +
    2041 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
 +
    2101 atgatttctg GAATTCGCGG CCGCTTCTAG AGtttacagc tagctcagtc ctagggactg
 +
    2161 tgctagcTGG AATTGTGAGC GGATAACAAT Tctatggact atgtttCTGT CACCGGATGT
 +
    2221 GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGtacc agtgaaagag gagaaaGGAT
 +
    2281 agatgccgat tagcccgatt gaaaccgttc cggttaaact gaaaccgggt atggatggtc
 +
    2341 cgaaagttaa acagtggcct ctgaccgaag aaaaaatcaa agcactggtt gaaatctgca
 +
    2401 ccgagatgga aaaagaaggc aaaattagca aaatcggtcc ggaaaatccg tataatacac
 +
    2461 cggtttttgc cattaagaaa aaagatagca ccaaatggcg caaactggtg gattttcgtg
 +
    2521 aactgaataa acgcacccag gatttttggg aagttcagct gggtattccg catccggcag
 +
    2581 gtctgaaaca gaaaaaaagc gttaccgttc tggatgttgg tgatgcatat tttagcgttc
 +
    2641 cgctggataa agatttccgt aaatataccg catttaccat cccgagcatt aataacgaaa
 +
    2701 caccgggtat tcgctatcag tataatgttc tgccgcaggg ttggaaaggt agtccggcaa
 +
    2761 tttttcagtg tagcatgacc aaaattctgg aaccgtttcg taaacagaat ccggatattg
 +
    2821 tgatctacca gtatatggat gatctgtatg ttggtagcga tctggaaatt ggtcagcatc
 +
    2881 gtaccaaaat tgaagaactg cgtcagcatc tgctgcgttg gggttttacc acaccggata
 +
    2941 aaaaacatca gaaagaaccg ccttttctgt ggatgggtta tgaactgcat ccggataaat
 +
    3001 ggaccgttca gccgattgtt ctgccggaaa aagatagctg gaccgttaat gatattcaga
 +
    3061 aactggtggg taaactgaat tgggcaagcc agatttatgc cggtattaaa gttcgtcagc
 +
    3121 tgtgtaaact gctgcgtggc accaaagcac tgaccgaagt tgttccgctg acagaagaag
 +
    3181 cagaactgga actggcagaa aatcgtgaaa ttctgaaaga accggttcac ggcgtttatt
 +
    3241 atgatccgag caaagatctg attgccgaaa ttcagaaaca gggtcagggt cagtggacct
 +
    3301 atcagattta tcaagaaccg tttaaaaacc tgaaaaccgg caaatatgca cgtatgaaag
 +
    3361 gtgcacatac caacgatgtt aaacagctga ccgaagcagt tcagaaaatt gcaaccgaaa
 +
    3421 gcattgtgat ttggggtaaa accccgaaat tcaaactgcc gattcagaaa gaaacctggg
 +
    3481 aagcatggtg gaccgaatat tggcaggcaa cctggattcc ggaatgggaa tttgttaata
 +
    3541 cccctccgct ggttaaactg tggtatcagc tggaaaaaga accgattatt ggtgccgaaa
 +
    3601 ccttttatgt tgatggtgca gccaatcgtg aaaccaaact gggtaaagca ggttatgtta
 +
    3661 ccgatcgtgg tcgtcagaaa gtggtgccgc tgaccgatac caccaatcag aaaaccgaac
 +
    3721 tacaggcaat tcatctggca ctacaggata gcggtctgga agttaatatt gttaccgata
 +
    3781 gccagtatgc cctgggtatt attcaggcac agccggataa aagcgaaagc gaactggtta
 +
    3841 gccagattat tgaacagctg atcaaaaaag aaaaagtgta cctggcatgg gttccggcac
 +
    3901 ataaaggtat tggtggtaat gaacaggttg atggtctggt tagcgcaggt attcgtaaag
 +
    3961 ttctgtaata ttagtgaaag aggagaaata ctagatgccg attagcccga ttgaaaccgt
 +
    4021 tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc ctctgaccga
 +
    4081 agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag gcaaaattag
 +
    4141 caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga aaaaagatag
 +
    4201 caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc aggatttttg
 +
    4261 ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa gcgttaccgt
 +
    4321 tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc gtaaatatac
 +
    4381 cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc agtataatgt
 +
    4441 tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga ccaaaattct
 +
    4501 ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg atgatctgta
 +
    4561 tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac tgcgtcagca
 +
    4621 tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac cgccttttct
 +
    4681 gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg ttctgccgga
 +
    4741 aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga attgggcaag
 +
    4801 ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg gcaccaaagc
 +
    4861 actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag aaaatcgtga
 +
    4921 aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc tgattgccga
 +
    4981 aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac cgtttaaaaa
 +
    5041 cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg ttaaacagct
 +
    5101 gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta aaaccccgaa
 +
    5161 attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat attggcaggc
 +
    5221 aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac tgtggtatca
 +
    5281 gctggaaaaa gaaccgatta ttggtgccga aaccttttaa gatcgctact agagccaggc
 +
    5341 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
 +
    5401 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
 +
    5461 atatactaga agcggcTTTA CAGCTAGCTC AGTCCTAGGG ACTGTGCTAG CTGGAATTGT
 +
    5521 GAGCGGATAA CAATTCGTTG CAACTAAAGA GGAGAAAGGT ACCATGGGGC ATAATCACAA
 +
    5581 CCACAATCAC AATCATAACC ACAATGGAGG TGACGATGAC GATAAGGGTG GCCGCCAGGC
 +
    5641 CTGGATCCCA GCCGGCCCCA Aggcccgatc gatgggacca atggggcagc ccctgcaagt
 +
    5701 gttgacccta aatatagaag atgagtatcg gctacatgag acctcaaaag agccagatgt
 +
    5761 ttctctaggg tccacatggc tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat
 +
    5821 gggactggca gttcgccaag ctcctctgat catacctctg aaagcaacct ctacccccgt
 +
    5881 gtccataaaa caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca
 +
    5941 gagactgttg gaccagggaa tactggtacc ctgccagtcc ccctggaaca cgcccctgct
 +
    6001 acccgttaag aaaccaggga ctaatgatta taggcctgtc caggatctga gagaagtcaa
 +
    6061 caagcgggtg gaagacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct
 +
    6121 cccaccgtcc caccagtggt acactgtgct tgatttaaag gatgcctttt tctgcctgag
 +
    6181 actccacccc accagtcagc ctctcttcgc ctttgagtgg agagatccag agatgggaat
 +
    6241 ctcaggacaa ttgacctgga ccagactccc acagggtttc aaaaacagtc ccaccctgtt
 +
    6301 tgatgaggca ctgcacagag acctagcaga cttccggatc cagcacccag acttgatcct
 +
    6361 gctacagtac gtggatgact tactgctggc cgccacttct gagctagact gccaacaagg
 +
    6421 tactcgggcc ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc
 +
    6481 ccaaatttgc cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg
 +
    6541 gctgactgag gccagaaaag agactgtgat ggggcagcct actccgaaga cccctcgaca
 +
    6601 actaagggag ttcctaggga cggcaggctt ctgtcgcctc tggatccctg ggtttgcaga
 +
    6661 aatggcagcc cccttgtacc ctctcaccaa aacggggact ctgtttaatt ggggcccaga
 +
    6721 ccaacaaaag gcctatcaag aaatcaagca agctcttcta actgccccag ccctggggtt
 +
    6781 gccagatttg actaagccct ttgaactctt tgtcgacgag aagcagggct acgccaaagg
 +
    6841 tgtcctaacg caaaaactgg gaccttggcg tcggccggtg gcctacctgt ccaaaaagct
 +
    6901 agacccagta gcagctgggt ggcccccttg cctacggatg gtagcagcca ttgccgtact
 +
    6961 gacaaaggat gcaggcaagc taaccatggg acagccactt gtcattctgg ccccccatgc
 +
    7021 agtagaggca cttgtcaaac aaccccccga ccgctggctt tccaacgccc ggatgactca
 +
    7081 ctatcaggcc ttgcttttgg acacggaccg ggtccagttc ggaccggtgg tagccctgaa
 +
    7141 cccggctacg ctgctcccac tgcctgagga agggctgcaa cacaactgcc ttgatatcct
 +
    7201 ggccgaagcc cacggaaccc gacccgacct aacggaccag ccgctcccag acgccgacca
 +
    7261 cacctggtac acggatggaa gcagtctctt acaagaggga cagcgtaagg cgggagctgc
 +
    7321 ggtgaccacc gagaccgagg taatctgggc taaagccctg ccagccggga catccgctca
 +
    7381 gcgggctgaa ctgatagcac tcacccaggc cctaaagatg gcagaaggta agaagctaaa
 +
    7441 tgtttatact gatagccgtt atgcttttgc tactgcccat atccatggag aaatatacag
 +
    7501 aaggcgtggg ttgctcacat cagaaggcaa agagatcaaa aataaagacg agatctttaa
 +
    7561 atgacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
 +
    7621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
 +
    7681 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatata ataaatgtcc
 +
    7741 agaAGGGcct tcaggcatgc aagcctctgg aggcatcaaa taaaacgaaa ggctcagtcg
 +
    7801 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
 +
    7861 aatTACTAGT AGCGGCCGCT GCAGAGTC
 +
//`
 +
       
 +
sequences.seq_Updated_Assembly_in_Kan = {};
 +
sequences.seq_Updated_Assembly_in_Kan.varName = "seq_Updated_Assembly_in_Kan";
 +
sequences.seq_Updated_Assembly_in_Kan.name = "Updated Assembly in Kan";
 +
sequences.seq_Updated_Assembly_in_Kan.seqType = "both";
 +
sequences.seq_Updated_Assembly_in_Kan.comp = true;
 +
sequences.seq_Updated_Assembly_in_Kan.data = `LOCUS      Updated_Assembly_in_Kan 7935 bp ds-DNA    circular    24-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    terminator      7746..7789
 +
                    /label="bacterial terminator"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    1741..3066
 +
                    /label="HIVRT P51"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    5626..5630
 +
                    /label="BioBrick suffix"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    RBS            11..22
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    misc_feature    3358..3372
 +
                    /label="Enterokinase Site"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    7792..7792
 +
                    /label="BioBrick prefix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    Biobricking    7793..7813
 +
                    /label="BioBrick Prefix "
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    CDS            complement(6704..7519)
 +
                    /label="KanR"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    Scar            3073..3080
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind    7427..7446
 +
                    /label="Kan-R"
 +
                    /ApEinfo_revcolor=#c6c9d1
 +
                    /ApEinfo_fwdcolor=#c6c9d1
 +
    misc_binding    7850..7872
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    misc_feature    29..1714
 +
                    /label="HIVRT p66"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Scar            5336..5343
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      3176..3203
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    terminator      3089..3160
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    CDS            3316..3351
 +
                    /label="6xHN"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    source          5626..7792
 +
                    /label="source"
 +
                    /ApEinfo_revcolor=#b4abac
 +
                    /ApEinfo_fwdcolor=#b4abac
 +
    terminator      5439..5466
 +
                    /label="T7Te terminator"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    misc_feature    7814..7848
 +
                    /label="BBa_J23109'"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    misc_feature    join(7888..7935,1..2)
 +
                    /label="sTRSV HHRz"
 +
                    /ApEinfo_revcolor=#f8d3a9
 +
                    /ApEinfo_fwdcolor=#f8d3a9
 +
    terminator      5523..5609
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#b7e6d7
 +
                    /ApEinfo_fwdcolor=#b7e6d7
 +
    primer_bind    7724..7742
 +
                    /label="pBRforEco"
 +
                    /ApEinfo_revcolor=#75c6a9
 +
                    /ApEinfo_fwdcolor=#75c6a9
 +
    RBS            1723..1734
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#c7b0e3
 +
                    /ApEinfo_fwdcolor=#c7b0e3
 +
    Biobricking    5610..5625
 +
                    /label="BioBrick Suffix"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
    None            3223..3257
 +
                    /label="'BBa_J23109'"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    primer_bind    7511..7530
 +
                    /label="pENTR-R"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    primer_bind    complement(5963..5982)
 +
                    /label="pBR322ori-F"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    Scar            3161..3168
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    RBS            3292..3303
 +
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
 +
                    /ApEinfo_revcolor=#faac61
 +
                    /ApEinfo_fwdcolor=#faac61
 +
    rep_origin      complement(5883..6471)
 +
                    /label="ori"
 +
                    /ApEinfo_revcolor=#85dae9
 +
                    /ApEinfo_fwdcolor=#85dae9
 +
    primer_bind    complement(5712..5729)
 +
                    /label="L4440"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
    terminator      5631..5688
 +
                    /label="his operon terminator"
 +
                    /ApEinfo_revcolor=#b1ff67
 +
                    /ApEinfo_fwdcolor=#b1ff67
 +
    Scar            5424..5431
 +
                    /label="BioBrick Scar"
 +
                    /ApEinfo_revcolor=#9eafd2
 +
                    /ApEinfo_fwdcolor=#9eafd2
 +
    terminator      5352..5423
 +
                    /label="rrnB T1 terminator"
 +
                    /ApEinfo_revcolor=#f58a5e
 +
                    /ApEinfo_fwdcolor=#f58a5e
 +
    misc_feature    3310..5310
 +
                    /label="MLRT ORF"
 +
                    /ApEinfo_revcolor=#ffef86
 +
                    /ApEinfo_fwdcolor=#ffef86
 +
    misc_binding    3259..3281
 +
                    /label="LacO"
 +
                    /ApEinfo_revcolor=#84b0dc
 +
                    /ApEinfo_fwdcolor=#84b0dc
 +
ORIGIN
 +
        1 AGtaccagtg aaagaggaga aaGGATagat gccgattagc ccgattgaaa ccgttccggt
 +
      61 taaactgaaa ccgggtatgg atggtccgaa agttaaacag tggcctctga ccgaagaaaa
 +
      121 aatcaaagca ctggttgaaa tctgcaccga gatggaaaaa gaaggcaaaa ttagcaaaat
 +
      181 cggtccggaa aatccgtata atacaccggt ttttgccatt aagaaaaaag atagcaccaa
 +
      241 atggcgcaaa ctggtggatt ttcgtgaact gaataaacgc acccaggatt tttgggaagt
 +
      301 tcagctgggt attccgcatc cggcaggtct gaaacagaaa aaaagcgtta ccgttctgga
 +
      361 tgttggtgat gcatatttta gcgttccgct ggataaagat ttccgtaaat ataccgcatt
 +
      421 taccatcccg agcattaata acgaaacacc gggtattcgc tatcagtata atgttctgcc
 +
      481 gcagggttgg aaaggtagtc cggcaatttt tcagtgtagc atgaccaaaa ttctggaacc
 +
      541 gtttcgtaaa cagaatccgg atattgtgat ctaccagtat atggatgatc tgtatgttgg
 +
      601 tagcgatctg gaaattggtc agcatcgtac caaaattgaa gaactgcgtc agcatctgct
 +
      661 gcgttggggt tttaccacac cggataaaaa acatcagaaa gaaccgcctt ttctgtggat
 +
      721 gggttatgaa ctgcatccgg ataaatggac cgttcagccg attgttctgc cggaaaaaga
 +
      781 tagctggacc gttaatgata ttcagaaact ggtgggtaaa ctgaattggg caagccagat
 +
      841 ttatgccggt attaaagttc gtcagctgtg taaactgctg cgtggcacca aagcactgac
 +
      901 cgaagttgtt ccgctgacag aagaagcaga actggaactg gcagaaaatc gtgaaattct
 +
      961 gaaagaaccg gttcacggcg tttattatga tccgagcaaa gatctgattg ccgaaattca
 +
    1021 gaaacagggt cagggtcagt ggacctatca gatttatcaa gaaccgttta aaaacctgaa
 +
    1081 aaccggcaaa tatgcacgta tgaaaggtgc acataccaac gatgttaaac agctgaccga
 +
    1141 agcagttcag aaaattgcaa ccgaaagcat tgtgatttgg ggtaaaaccc cgaaattcaa
 +
    1201 actgccgatt cagaaagaaa cctgggaagc atggtggacc gaatattggc aggcaacctg
 +
    1261 gattccggaa tgggaatttg ttaatacccc tccgctggtt aaactgtggt atcagctgga
 +
    1321 aaaagaaccg attattggtg ccgaaacctt ttatgttgat ggtgcagcca atcgtgaaac
 +
    1381 caaactgggt aaagcaggtt atgttaccga tcgtggtcgt cagaaagtgg tgccgctgac
 +
    1441 cgataccacc aatcagaaaa ccgaactaca ggcaattcat ctggcactac aggatagcgg
 +
    1501 tctggaagtt aatattgtta ccgatagcca gtatgccctg ggtattattc aggcacagcc
 +
    1561 ggataaaagc gaaagcgaac tggttagcca gattattgaa cagctgatca aaaaagaaaa
 +
    1621 agtgtacctg gcatgggttc cggcacataa aggtattggt ggtaatgaac aggttgatgg
 +
    1681 tctggttagc gcaggtattc gtaaagttct gtaatattag tgaaagagga gaaatactag
 +
    1741 atgccgatta gcccgattga aaccgttccg gttaaactga aaccgggtat ggatggtccg
 +
    1801 aaagttaaac agtggcctct gaccgaagaa aaaatcaaag cactggttga aatctgcacc
 +
    1861 gagatggaaa aagaaggcaa aattagcaaa atcggtccgg aaaatccgta taatacaccg
 +
    1921 gtttttgcca ttaagaaaaa agatagcacc aaatggcgca aactggtgga ttttcgtgaa
 +
    1981 ctgaataaac gcacccagga tttttgggaa gttcagctgg gtattccgca tccggcaggt
 +
    2041 ctgaaacaga aaaaaagcgt taccgttctg gatgttggtg atgcatattt tagcgttccg
 +
    2101 ctggataaag atttccgtaa atataccgca tttaccatcc cgagcattaa taacgaaaca
 +
    2161 ccgggtattc gctatcagta taatgttctg ccgcagggtt ggaaaggtag tccggcaatt
 +
    2221 tttcagtgta gcatgaccaa aattctggaa ccgtttcgta aacagaatcc ggatattgtg
 +
    2281 atctaccagt atatggatga tctgtatgtt ggtagcgatc tggaaattgg tcagcatcgt
 +
    2341 accaaaattg aagaactgcg tcagcatctg ctgcgttggg gttttaccac accggataaa
 +
    2401 aaacatcaga aagaaccgcc ttttctgtgg atgggttatg aactgcatcc ggataaatgg
 +
    2461 accgttcagc cgattgttct gccggaaaaa gatagctgga ccgttaatga tattcagaaa
 +
    2521 ctggtgggta aactgaattg ggcaagccag atttatgccg gtattaaagt tcgtcagctg
 +
    2581 tgtaaactgc tgcgtggcac caaagcactg accgaagttg ttccgctgac agaagaagca
 +
    2641 gaactggaac tggcagaaaa tcgtgaaatt ctgaaagaac cggttcacgg cgtttattat
 +
    2701 gatccgagca aagatctgat tgccgaaatt cagaaacagg gtcagggtca gtggacctat
 +
    2761 cagatttatc aagaaccgtt taaaaacctg aaaaccggca aatatgcacg tatgaaaggt
 +
    2821 gcacatacca acgatgttaa acagctgacc gaagcagttc agaaaattgc aaccgaaagc
 +
    2881 attgtgattt ggggtaaaac cccgaaattc aaactgccga ttcagaaaga aacctgggaa
 +
    2941 gcatggtgga ccgaatattg gcaggcaacc tggattccgg aatgggaatt tgttaatacc
 +
    3001 cctccgctgg ttaaactgtg gtatcagctg gaaaaagaac cgattattgg tgccgaaacc
 +
    3061 ttttaagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
 +
    3121 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
 +
    3181 accttcgggt gggcctttct gcgtttatat actagaagcg gcTTTACAGC TAGCTCAGTC
 +
    3241 CTAGGGACTG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCGTTGCAAC TAAAGAGGAG
 +
    3301 AAAGGTACCA TGGGGCATAA TCACAACCAC AATCACAATC ATAACCACAA TGGAGGTGAC
 +
    3361 GATGACGATA AGGGTGGCCG CCAGGCCTGG ATCCCAGCCG GCCCCAAggc ccgatcgatg
 +
    3421 ggaccaatgg ggcagcccct gcaagtgttg accctaaata tagaagatga gtatcggcta
 +
    3481 catgagacct caaaagagcc agatgtttct ctagggtcca catggctgtc tgattttcct
 +
    3541 caggcctggg cggaaaccgg gggcatggga ctggcagttc gccaagctcc tctgatcata
 +
    3601 cctctgaaag caacctctac ccccgtgtcc ataaaacaat accccatgtc acaagaagcc
 +
    3661 agactgggga tcaagcccca catacagaga ctgttggacc agggaatact ggtaccctgc
 +
    3721 cagtccccct ggaacacgcc cctgctaccc gttaagaaac cagggactaa tgattatagg
 +
    3781 cctgtccagg atctgagaga agtcaacaag cgggtggaag acatccaccc caccgtgccc
 +
    3841 aacccttaca acctcttgag cgggctccca ccgtcccacc agtggtacac tgtgcttgat
 +
    3901 ttaaaggatg cctttttctg cctgagactc caccccacca gtcagcctct cttcgccttt
 +
    3961 gagtggagag atccagagat gggaatctca ggacaattga cctggaccag actcccacag
 +
    4021 ggtttcaaaa acagtcccac cctgtttgat gaggcactgc acagagacct agcagacttc
 +
    4081 cggatccagc acccagactt gatcctgcta cagtacgtgg atgacttact gctggccgcc
 +
    4141 acttctgagc tagactgcca acaaggtact cgggccctgt tacaaaccct agggaacctc
 +
    4201 gggtatcggg cctcggccaa gaaagcccaa atttgccaga aacaggtcaa gtatctgggg
 +
    4261 tatcttctaa aagagggtca gagatggctg actgaggcca gaaaagagac tgtgatgggg
 +
    4321 cagcctactc cgaagacccc tcgacaacta agggagttcc tagggacggc aggcttctgt
 +
    4381 cgcctctgga tccctgggtt tgcagaaatg gcagccccct tgtaccctct caccaaaacg
 +
    4441 gggactctgt ttaattgggg cccagaccaa caaaaggcct atcaagaaat caagcaagct
 +
    4501 cttctaactg ccccagccct ggggttgcca gatttgacta agccctttga actctttgtc
 +
    4561 gacgagaagc agggctacgc caaaggtgtc ctaacgcaaa aactgggacc ttggcgtcgg
 +
    4621 ccggtggcct acctgtccaa aaagctagac ccagtagcag ctgggtggcc cccttgccta
 +
    4681 cggatggtag cagccattgc cgtactgaca aaggatgcag gcaagctaac catgggacag
 +
    4741 ccacttgtca ttctggcccc ccatgcagta gaggcacttg tcaaacaacc ccccgaccgc
 +
    4801 tggctttcca acgcccggat gactcactat caggccttgc ttttggacac ggaccgggtc
 +
    4861 cagttcggac cggtggtagc cctgaacccg gctacgctgc tcccactgcc tgaggaaggg
 +
    4921 ctgcaacaca actgccttga tatcctggcc gaagcccacg gaacccgacc cgacctaacg
 +
    4981 gaccagccgc tcccagacgc cgaccacacc tggtacacgg atggaagcag tctcttacaa
 +
    5041 gagggacagc gtaaggcggg agctgcggtg accaccgaga ccgaggtaat ctgggctaaa
 +
    5101 gccctgccag ccgggacatc cgctcagcgg gctgaactga tagcactcac ccaggcccta
 +
    5161 aagatggcag aaggtaagaa gctaaatgtt tatactgata gccgttatgc ttttgctact
 +
    5221 gcccatatcc atggagaaat atacagaagg cgtgggttgc tcacatcaga aggcaaagag
 +
    5281 atcaaaaata aagacgagat ctttaaatga cgctgatagt gctagtgtag atcgctacta
 +
    5341 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
 +
    5401 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
 +
    5461 tctgcgttta tatataataa atgtccagaA GGGccttcag gcatgcaagc ctctggaggc
 +
    5521 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
 +
    5581 cggtgaacgc tctcctgagt aggacaaatT ACTAGTAGCG GCCGCtgcag tccggcaaaa
 +
    5641 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
 +
    5701 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
 +
    5761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
 +
    5821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
 +
    5881 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
 +
    5941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
 +
    6001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
 +
    6061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
 +
    6121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
 +
    6181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
 +
    6241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
 +
    6301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
 +
    6361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
 +
    6421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
 +
    6481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
 +
    6541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
 +
    6601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgagtcc cgtcaagtca
 +
    6661 gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
 +
    6721 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
 +
    6781 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
 +
    6841 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
 +
    6901 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
 +
    6961 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
 +
    7021 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
 +
    7081 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
 +
    7141 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
 +
    7201 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
 +
    7261 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
 +
    7321 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
 +
    7381 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
 +
    7441 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt
 +
    7501 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
 +
    7561 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
 +
    7621 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagctc gagtgccact
 +
    7681 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
 +
    7741 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggAATTCGCG
 +
    7801 GCCGCTTCTA GAGtttacag ctagctcagt cctagggact gtgctagcTG GAATTGTGAG
 +
    7861 CGGATAACAA TTctatggac tatgtttCTG TCACCGGATG TGCTTTCCGG TCTGATGAGT
 +
    7921 CCGTGAGGAC GAAAC
 +
//`
 +
       
 +
sequences.seq_ZFc_codon_optimized_1 = {};
 +
sequences.seq_ZFc_codon_optimized_1.varName = "seq_ZFc_codon_optimized_1";
 +
sequences.seq_ZFc_codon_optimized_1.name = "ZFc (codon optimized) (1)";
 +
sequences.seq_ZFc_codon_optimized_1.seqType = "linear";
 +
sequences.seq_ZFc_codon_optimized_1.comp = true;
 +
sequences.seq_ZFc_codon_optimized_1.data = `LOCUS      ZFc_(codon_optimized)    331 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    18..311
 +
                    /label="ZFc with linker"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
ORIGIN
 +
        1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA
 +
      61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG
 +
      121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA
 +
      181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA
 +
      241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC
 +
      301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T
 +
//`
 +
       
 +
sequences.seq_ZFc_codon_optimized = {};
 +
sequences.seq_ZFc_codon_optimized.varName = "seq_ZFc_codon_optimized";
 +
sequences.seq_ZFc_codon_optimized.name = "ZFc (codon optimized)";
 +
sequences.seq_ZFc_codon_optimized.seqType = "linear";
 +
sequences.seq_ZFc_codon_optimized.comp = true;
 +
sequences.seq_ZFc_codon_optimized.data = `LOCUS      ZFc_(codon_optimized)    331 bp ds-DNA    linear      25-OCT-2020
 +
DEFINITION  .
 +
FEATURES            Location/Qualifiers
 +
    misc_feature    18..311
 +
                    /label="ZFc with linker"
 +
                    /ApEinfo_revcolor=#ff9ccd
 +
                    /ApEinfo_fwdcolor=#ff9ccd
 +
ORIGIN
 +
        1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA
 +
      61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG
 +
      121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA
 +
      181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA
 +
      241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC
 +
      301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T
 
//`
 
//`

Latest revision as of 21:16, 25 October 2020

/*This file is used to store the text from the .gb files in a form that's readable by other scripts*/ var sequences = {}; sequences.seq_2x_Res_6x_CoA = {}; sequences.seq_2x_Res_6x_CoA.varName = "seq_2x_Res_6x_CoA"; sequences.seq_2x_Res_6x_CoA.name = "(2x Res 6x CoA)"; sequences.seq_2x_Res_6x_CoA.seqType = "both"; sequences.seq_2x_Res_6x_CoA.comp = true; sequences.seq_2x_Res_6x_CoA.data = `LOCUS (2x_Res,_6x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_binding        2668..2690
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    3089..3097
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    3078..3086
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    3034..3042
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Scar                2923..2930
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    misc_binding        2967..2989
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    gBlock              2501..2625
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2702..2710)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    3100..3108
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site 2744..2853
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(3410..3418)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Promoter            2931..2965
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(3421..3429)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    HIV-RT Binding Site join(3518..3521,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(3465..3473)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(3443..3451)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2436..2444
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    complement(3509..3517)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    3122..3130
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(3377..3385)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    gBlock              3187..3311
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    3023..3031
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    3045..3053
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(3487..3495)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Scar                3310..3317
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Zinc Finger Site    complement(2691..2699)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    2425..2433
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2414..2422
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    3001..3009
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(3498..3506)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    3012..3020
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(3454..3462)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Promoter            3318..3352
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Terminator          3241..3309
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(3399..3407)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Terminator          2555..2623
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(3432..3440)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(3388..3396)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    3056..3064
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    3067..3075
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Scar                2624..2631
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Terminator          2854..2922
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    3111..3119
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2990..2998
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Promoter            2632..2666
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2724..2732)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2735..2743)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    HIV-RT Binding Site 2445..2554
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    HIV-RT Binding Site 3131..3240
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(3476..3484)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2403..2411
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    misc_binding        3354..3376
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2713..2721)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    gBlock              2800..2924
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
    2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
    2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
    2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
    2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
    2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAAAAA ACGTGGCGCC
    2761 CGAACAGGGA CGGATAGCTC AGTCGGTAGA GCATCAGACT TTTAATCTGA GGGTCCAGGG
    2821 TTCAAGTCCC TGTTCGGGCG CCACGTTTTT TTTattagca gaaagtcaaa agcctccgac
    2881 cggaggcttt tgactaaaac ttcccttggg gttatcattg ggtactagag TTGACGGCTA
    2941 GCTCAGTCCT AGGTACAGTG CTAGCTGGAA TTGTGAGCGG ATAACAATTG AGGACGGCAT
    3001 GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC
    3061 TGGGATGTCG ATGCCATGCG GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
    3121 TGCGGCTGGG AAAAAAAACG TGGCGCCCGA ACAGGGACGG ATAGCTCAGT CGGTAGAGCA
    3181 TCAGACTTTT AATCTGAGGG TCCAGGGTTC AAGTCCCTGT TCGGGCGCCA CGTTTTTTTT
    3241 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt
    3301 atcattgggt actagagTTG ACGGCTAGCT CAGTCCTAGG TACAGTGCTA GCTGGAATTG
    3361 TGAGCGGATA ACAATTCCCA GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT
    3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
    3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A

//`

sequences.seq_4x_Res_4x_CoA = {}; sequences.seq_4x_Res_4x_CoA.varName = "seq_4x_Res_4x_CoA"; sequences.seq_4x_Res_4x_CoA.name = "(4x Res 4x CoA)"; sequences.seq_4x_Res_4x_CoA.seqType = "both"; sequences.seq_4x_Res_4x_CoA.comp = true; sequences.seq_4x_Res_4x_CoA.data = `LOCUS (4x_Res,_4x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    Zinc Finger Site    3089..3097
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2779..2787)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    complement(3421..3429)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    3122..3130
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          2599..2667
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site    2447..2455
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    3133..3141
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Terminator          2942..3010
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    3155..3163
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(3454..3462)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2757..2765)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    complement(3465..3473)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    misc_binding        3055..3077
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    misc_binding        2712..2734
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Scar                2668..2675
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    3111..3119
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(3498..3506)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    HIV-RT Binding Site join(3518..3521,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2469..2477
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Scar                3354..3361
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    complement(2768..2776)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Promoter            3362..3396
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2735..2743)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2414..2422
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    3144..3152
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2812..2820)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Promoter            3019..3053
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(3509..3517)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    HIV-RT Binding Site 3175..3284
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2790..2798)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    3078..3086
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Terminator          3285..3353
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(3432..3440)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2403..2411
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    misc_binding        3398..3420
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Scar                3011..3018
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    gBlock              3231..3355
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2436..2444
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    complement(3443..3451)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    complement(2823..2831)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site    complement(3476..3484)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    gBlock              2545..2669
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2480..2488
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Zinc Finger Site    complement(2801..2809)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Promoter            2676..2710
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(3487..3495)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    3100..3108
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site 2489..2598
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    gBlock              2888..3012
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site 2832..2941
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2746..2754)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2425..2433
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2458..2466
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    3166..3174
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
    2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
    2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
    2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
    2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
    2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
    2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
    2821 ATGCCGTCCT CAAAAAAAAC GTGGCGCCCG AACAGGGACG GATAGCTCAG TCGGTAGAGC
    2881 ATCAGACTTT TAATCTGAGG GTCCAGGGTT CAAGTCCCTG TTCGGGCGCC ACGTTTTTTT
    2941 Tattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt
    3001 tatcattggg tactagagTT GACGGCTAGC TCAGTCCTAG GTACAGTGCT AGCTGGAATT
    3061 GTGAGCGGAT AACAATTGAG GACGGCATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
    3121 TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGAAAAAA
    3181 AACGTGGCGC CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG
    3241 AGGGTCCAGG GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa
    3301 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga
    3361 gTTGACGGCT AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT
    3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
    3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A

//`

sequences.seq_Addgene_109383_pSB1C3Lux = {}; sequences.seq_Addgene_109383_pSB1C3Lux.varName = "seq_Addgene_109383_pSB1C3Lux"; sequences.seq_Addgene_109383_pSB1C3Lux.name = "(Addgene 109383) pSB1C3-Lux"; sequences.seq_Addgene_109383_pSB1C3Lux.seqType = "both"; sequences.seq_Addgene_109383_pSB1C3Lux.comp = true; sequences.seq_Addgene_109383_pSB1C3Lux.data = `LOCUS (Addgene_#109383)_pSB1C 9707 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_109383_206254" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    terminator      1..58
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    rep_origin      complement(253..841)
                    /label="ori"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      1024..1118
                    /label="lambda t0 terminator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    promoter        2955..3239
                    /label="araBAD promoter"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    terminator      1982..2025
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2028..2049
                    /label="BioBrick prefix"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    CDS             complement(1139..1798)
                    /label="CmR"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    primer_bind     complement(82..99)
                    /label="L4440"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    join(9687..9707,1..2049)
                    /label="pSB1C3 Backbone Region"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    source          1..9707
                    /label="source"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer_bind     1960..1978
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    CDS             complement(2050..2928)
                    /label="araC"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    primer_bind     complement(333..352)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer_bind     3162..3181
                    /label="pBAD Forward"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    9687..9707
                    /label="BioBrick suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    9647..9650
                    /label="Overhang for end of pduD part"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer_bind     1713..1732
                    /label="CAT-R"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    primer          complement(2062..2093)
                    /label="pSB1C3 Reverse Primer"
                    /note="sequence: ACTGATCGTCTCTgttgtgaagaaaaagtgaatgatgtagccgtc"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    primer          9647..9670
                    /label="pSB1C3 Forward Primer"
                    /note="sequence: TATGATCGTCTCAgtcagaacagatgtttgccgatgc"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2

ORIGIN 

       1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
      61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
    1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
    1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
    1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
    1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
    1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
    1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
    1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
    1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
    1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
    1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
    1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
    1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
    1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
    1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
    1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
    1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
    1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
    2041 cttctagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
    2101 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
    2161 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
    2221 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
    2281 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
    2341 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
    2401 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
    2461 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
    2521 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
    2581 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
    2641 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
    2701 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
    2761 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
    2821 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
    2881 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
    2941 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
    3001 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
    3061 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
    3121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
    3181 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
    3241 cccgtttttt tgggctagca gggaaaataa tgaataaatg tattccaatg ataattaatg
    3301 gaatgattca agattttgat aattatgcat ataaagaagt taaactaaat aatgataata
    3361 gagtaaaatt atctgtcatt actgaaagtt cagtttcaaa aacattaaat atcaaagata
    3421 gaattaatct aaatttaaat cagattgtga attttttata taccgttggt caacgatgga
    3481 aaagtgaaga atataatcgg cgacgaacct atatccgtga gttaaaaaca tatcttggtt
    3541 attctgatga aatggcaaga ttagaagcga attggattgc aatgttattg tgctctaaaa
    3601 gtgctttgta tgacattgtt aattatgatt tgggctctat acacgtatta gatgaatggc
    3661 ttccacgtgg tgattgctat gttaaagcac aaccgaaagg tgtttctgtt cacttgttag
    3721 ctggtaatgt tccattatca ggagtgacat ctattttgcg tgctatttta acaaaaaatg
    3781 agtgcattat taaaacttcg tcttcagatc cttttactgc aaacgcttta gtttccagtt
    3841 ttattgatgt taatgcagac catccaataa ccaaatcaat gtctgttatg tattggccgc
    3901 atgatgaaga tatgactcta tctcaaagaa taatgaatca tgccgacgtg gttattgctt
    3961 ggggtggaga cgaggcgatt aaatgggcgg taaaatattc accaccgcat gtcgatattc
    4021 tgaaatttgg accaaagaaa agcttaagta ttattgaagc tcctaaagat atagaagccg
    4081 cagcaatggg ggttgctcat gatatttgtt tctatgacca gcaagcctgc ttctctactc
    4141 aagacgttta ttatatagga gataatttac ctttattttt aaatgaactt gaaaaacagc
    4201 tagatcgata cgcgaaaatt ttaccaaaag gttcaaatag ttttgatgaa aaagcggcgt
    4261 ttactcttac tgaaaaagaa agtctatttg ctggatatga agtgagaaag ggagataagc
    4321 aagcttggtt aatagtcgta tcacctacaa atagctttgg aaatcaaccg ctatcacgaa
    4381 gtgtgtatgt tcatcaagta tctgatatta aagagataat tccttttgtt aataaaaata
    4441 gaacacaaac tgtttctatt tatccttggg aagcgtcatt aaaatatcga gataaattag
    4501 caagaagtgg agttgaaaga attgttgaat caggcatgaa taatattttc agagttggag
    4561 gggctcatga ttcattatct cctctccagt acctagttag gtttgtatcg catgagagac
    4621 catttaatta tacgacaaaa gatgttgcgg ttgaaatcga acaaacacgt tacttagagg
    4681 aagataagtt tttagttttt gtcccatagt taaaggaaat tatatgaaag atgaaagtgc
    4741 tttttttacg attgatcaca tcatcaagct tgataatggt cagtctatcc gagtttggga
    4801 aacactccct aaaaagaacg taccagagaa aaaacataca atacttattg cttcgggttt
    4861 tgctagaaga atggatcatt ttgcaggtct tgctgagtat ttatctacta acggttttca
    4921 tgtcattcgc tacgattctt tgcatcatgt tggattaagc agtggatgta taaatgaatt
    4981 tacgatgtcg attggaaaaa atagcctgct tacagtcgta gattggctta cagatcatgg
    5041 tgtcgaacga atagggctga ttgctgctag tttgtcagcg agaatcgcct atgaggtagt
    5101 aaataaaatt aaattatcat ttttaattac ggccgtaggt gtcgttaatc ttagagatac
    5161 attagaaaaa gcattggagt atgactattt gcaattacct atttcagagt taccagaaga
    5221 tcttgacttt gaaggtcata atttaggatc ggaggtcttt gttacagatt gctttaaaca
    5281 taattgggac acgttagact cgacacttaa tagtgttaaa ggattagcga ttccatttat
    5341 tgcttttact gcaaacgatg atagttgggt aaagcaaagt gaagttatag agctcattga
    5401 tagcattgaa tctagtaatt gtaagctcta ttcgctaatt ggaagttcac atgatcttgg
    5461 ggaaaatttg gttgtattaa gaaattttta tcaatcagtg acgaaggcag ccttagcatt
    5521 agatgatggt ttattggatt tagagataga cattattgaa cctcgatttg aggacgttac
    5581 aagtattact gttaaggagc gtagattaaa aaatgaaatt gaaaatgaat tattagaatt
    5641 ggcttaaata aacagaatca ccaaaaagga atagagtatg aagtttggaa atatttgttt
    5701 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg
    5761 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga
    5821 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac
    5881 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga
    5941 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg
    6001 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac
    6061 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag
    6121 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc
    6181 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc
    6241 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa
    6301 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat
    6361 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa
    6421 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg
    6481 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa
    6541 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga
    6601 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc
    6661 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc
    6721 tttcttaaaa gaacctaaat aaattactta tttgatacta gagataataa ggaacaagtt
    6781 atgaaatttg gattattttt tctaaacttt cagaaagatg gaataacatc tgaagaaacg
    6841 ttggataata tggtaaagac tgtcacgtta attgattcaa ctaaatatca ttttaatact
    6901 gcctttgtta atgaacatca cttttcaaaa aatggtattg ttggagcacc tattaccgca
    6961 gctggttttt tattagggtt aacaaataaa ttacatattg gttcattaaa tcaagtaatt
    7021 accacccatc accctgtacg tgtagcagaa gaagccagtt tattagatca aatgtcagag
    7081 ggacgcttca ttcttggttt tagtgactgc gaaagtgatt tcgaaatgga attttttaga
    7141 cgtcatatct catcaaggca acaacaattt gaagcatgct atgaaataat taatgacgca
    7201 ttaactacag gttattgtca tccccaaaac gacttttatg attttccaaa ggtttcaatt
    7261 aatccacact gttacagtga gaatggacct aagcaatatg tatccgctac atcaaaagaa
    7321 gtcgtcatgt gggcagcgaa aaaggcactg cctttaacat ttaagtggga ggataattta
    7381 gaaaccaaag aacgctatgc aattctatat aataaaacag cacaacaata tggtattgat
    7441 atttcggatg ttgatcatca attaactgta attgcgaact taaatgctga tagaagtacg
    7501 gctcaagaag aagtgagaga atacttaaaa gactatatca ctgaaactta ccctcaaatg
    7561 gacagagatg aaaaaattaa ctgcattatt gaagagaatg cagttgggtc tcatgatgac
    7621 tattatgaat cgacaaaatt agcagtggaa aaaacagggt ctaaaaatat tttattatcc
    7681 tttgaatcaa tgtccgatat taaagatgta aaagatatta ttgatatgtt gaaccaaaaa
    7741 atcgaaatga atttaccata ataaaattaa aggcaatttc tatattagat tgccttttta
    7801 aatttctgtt gatattaggt attactggag agggtatgac tgtccatact gaatataaaa
    7861 gaaatcaaat cattgctagt tcagaaattg atgatcttat ctttatgacg aaaccacaag
    7921 agtggtcatt tgaagagcaa aaagaaatac gggataaatt agttcgtgag gctttttatt
    7981 ttcactacaa tagaaatgaa gaatatagaa attattgtat caatcagcat gtgagtgata
    8041 atttacacac tattgatgaa atacccgtgt ttccaacatc tgtttttaaa tataagaaat
    8101 tacatactgt cacagccgag gacattgaaa attggtatac aagtagtgga actcgtggag
    8161 taaaaagtca tattgcacgt gatcgtctta gcattgaacg cttgcttggt tctgtcaact
    8221 tcggaatgaa atacgttgga gattggtttg agcatcaaat ggaattgata aatttaggac
    8281 cagatagatt caatacaaat aatatttggt ttaaatatgt catgagtttg gtcgagttac
    8341 tttatccgac tgaatttaca gttgataatg acaaaataga ttttgaaaaa acagtaaaac
    8401 atctatttag aattaagaat agtaaaaaag acatttgctt aattgggcca ccattttttg
    8461 tgtatctttt gtgccaatat atgaaagaaa acaatattga atttaaagga ggagatagag
    8521 tacatattat tactggtgga ggatggaaat ctaatcagaa tgactcttta gatcgtgctg
    8581 attttaatca attattaatg gatactttcc aactcgacaa gattaatcaa attagagata
    8641 cctttaatca agttgagctt aatacttgtt tttttgaaga tgaatttcaa agaaaacatg
    8701 ttccaccgtg ggtatatgct cgggctcttg atcctgaaac cttgaaaccc gtagcagatg
    8761 gtgagatcgg gttgttaagt tatatggacg cctcatcaac tgcttaccct gcttttattg
    8821 ttactgatga tatcggtatt gtaaaagaaa ttagagaacc agatccttac ccaggggtaa
    8881 ctgttgagat tgttcggcgc ttaaatacac gtgcgcaaaa aggatgcgcg ctctctatgg
    8941 ctaatgtcat acaaaagaat atcaaggatt aagttatgat tgttgatggt agagtttcaa
    9001 aaatagtatt agcgtcaata agaaataata tatataaggt atttattact gtaaattcac
    9061 caataaagtt catcgctgga caatttgtaa tggtcacgat taatgggaaa aaatgccctt
    9121 tttcaattgc gaattgcccg acaaaaaatt acgaaataga attgcatatt ggtagttcga
    9181 atagagactg ctcattggat attatcgaat attttgtcga tgctcttgtt gaggaagtcg
    9241 caattgagtt agatgctccc catggaaacg cttggttacg gtctgaaagt aataacccat
    9301 tgctattaat tgcgggaggt acaggtttat catatataaa tagcattcta acaaattgct
    9361 taaataggaa tatacctcaa gatatttatc tttactgggg agtaaaagac agttctcttt
    9421 tgtatgaaga tgaagagtta ctaaacttat cactaaacaa caaaaacttt cattatattc
    9481 ctgttattga agataaaagt gaagaatgga tagggagaaa aggcactgtt cttgatgctg
    9541 tcatggaaga ttttactgat cttacttatt ttgatattta tgtttgtgga cccttcatga
    9601 tggctaaaac agcaaaagaa aaattaattg aagagaaaaa agcaaagtca gaacagatgt
    9661 ttgccgatgc ttttgcatac gtataatact agtagcggcc gctgcag

//`

sequences.seq_4CL_Intro_gblock = {}; sequences.seq_4CL_Intro_gblock.varName = "seq_4CL_Intro_gblock"; sequences.seq_4CL_Intro_gblock.name = "4CL Intro gblock"; sequences.seq_4CL_Intro_gblock.seqType = "linear"; sequences.seq_4CL_Intro_gblock.comp = true; sequences.seq_4CL_Intro_gblock.data = `LOCUS 4CL_Intro_gblock 480 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    437..466
                    /label="Spacer"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc_feature    40..73
                    /label="BBa_J23102"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc_feature    122..436
                    /label="ZIF268"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    98..111
                    /label="BBa_J61115"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    Biobricking     18..39
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_binding    74..97
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 CGTCAGGCTC TTCACACgaa ttcgcggccg cttctagagt gacagctagc tcagtcctag
      61 gtactgtgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGGGATA Ctaaatacat
     121 gATGCACCAC CATCACCATC ATCCCGGTGA GAAACCATAT GCGTGCCCGG TGGAAAGCTG
     181 TGATAGACGC TTTAGCCGTA GTGACGAACT GACCCGCCAC ATTCGAATAC ATACGGGGCA
     241 AAAACCGTTT CAGTGCCGCA TTTGTATGCG TAACTTCTCA CGGAGTGATC ATTTAACCAC
     301 TCATATTCGT ACTCATACAG GCGAAAAACC TTTTGCCTGC GACATCTGTG GTCGCAAATT
     361 CGCACGTTCC GATGAGCGCA AGCGTCACAC GAAAATCCAT ACCGGTGGCG GGAGCGGCGG
     421 CGGATCTGGT GGCTCGggtg gaagtggagg tggaagtgga ggtagtAGAA GAGCTGCAAG

//`

sequences.seq_4CL_Outro_gblock = {}; sequences.seq_4CL_Outro_gblock.varName = "seq_4CL_Outro_gblock"; sequences.seq_4CL_Outro_gblock.name = "4CL Outro gblock"; sequences.seq_4CL_Outro_gblock.seqType = "linear"; sequences.seq_4CL_Outro_gblock.comp = true; sequences.seq_4CL_Outro_gblock.data = `LOCUS 4CL_Outro_gblock 137 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    stem_loop       15..66
                    /label="T0 terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    Biobricking     67..87
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 TTCCGGGCTC TTCAttgttc agaacgctcg gtcttgcaca ccgggcgttt tttctttgtg
      61 agtccatact agtagcggcc gctgcagGGA AGAAGAGCtc caagagagtg aagggatgtc
     121 aagctgccat gAATTGA

//`

sequences.seq_ACC_intro_gblock = {}; sequences.seq_ACC_intro_gblock.varName = "seq_ACC_intro_gblock"; sequences.seq_ACC_intro_gblock.name = "ACC intro gblock"; sequences.seq_ACC_intro_gblock.seqType = "both"; sequences.seq_ACC_intro_gblock.comp = true; sequences.seq_ACC_intro_gblock.data = `LOCUS ACC_intro_gblock 2034 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    99..112
                    /label="RBS"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    40..74
                    /label="Promoter"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    113..1885
                    /label="ACCalpha"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    1889..2017
                    /label="Terminator"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_binding    75..98
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    18..39
                    /label="Prefix"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86

ORIGIN 

       1 AATGCTGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
      61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGAGGTGAC ATATGAGCGT
     121 GGAAACCCGT AAAATCACTA AAGTTTTGGT CGCGAACCGG GGTGAAATCG CTATTCGCGT
     181 TTTTCGTGCG GCCCGCGATG AGGGTATCGG CAGTGTTGCT GTCTACGCCG AGCCGGATGC
     241 CGATGCGCCA TTCGTGAGCT ATGCGGACGA AGCCTTTGCC CTGGGCGGCC AGACATCGGC
     301 CGAATCGTAT CTGGTAATCG ACAAAATCAT TGACGCCGCT CGTAAGAGCG GGGCTGATGC
     361 AATCCATCCC GGATACGGCT TCCTTGCAGA AAACGCGGAC TTCGCCGAAG CTGTTATAAA
     421 CGAAGGGCTA ATTTGGATTG GCCCGTCCCC AGAGAGCATC AGATCCTTAG GAGACAAAGT
     481 AACAGCGCGG CATATTGCGG ATACCGCAAA GGCGCCGATG GCACCCGGGA CGAAAGAGCC
     541 CGTGAAAGAT GCCGCCGAAG TCGTGGCTTT TGCAGAGGAG TTTGGGCTGC CGATCGCTAT
     601 CAAAGCAGCC TTTGGTGGTG GTGGCCGTGG AATGAAGGTG GCCTACAAAA TGGAAGAAGT
     661 CGCGGATCTG TTTGAGTCTG CGACCCGTGA AGCGACCGCC GCTTTCGGTC GAGGTGAGTG
     721 CTTTGTGGAA CGGTATCTGG ACAAAGCGCG CCACGTGGAA GCGCAAGTCA TCGCAGATAA
     781 ACATGGAAAC GTCGTAGTGG CGGGCACCCG CGACTGCTCG CTTCAGCGCC GTTTTCAAAA
     841 ACTCGTGGAA GAAGCGCCGG CACCGTTTCT CACTGATGAC CAAAGAGAGC GTTTACACTC
     901 AAGTGCAAAG GCGATTTGTA AAGAAGCTGG CTATTATGGT GCTGGCACTG TTGAATACTT
     961 GGTTGGTTCT GATGGACTCA TCTCGTTCCT GGAAGTGAAT ACCCGTCTGC AAGTCGAACA
    1021 CCCTGTGACG GAAGAAACGA CAGGTATCGA CCTGGTTCGC GAAATGTTTC GTATTGCGGA
    1081 AGGTCATGAA CTGTCTATTA AAGAAGATCC AGCACCCCGC GGCCACGCCT TCGAGTTCCG
    1141 TATTAACGGC GAGGATGCCG GCTCAAACTT CATGCCGGCT CCTGGAAAGA TTACTAGCTA
    1201 CCGAGAACCA CAGGGGCCTG GCGTTCGTAT GGACTCCGGC GTCGTGGAGG GGAGCGAAAT
    1261 ATCTGGTCAG TTTGACAGTA TGTTGGCAAA GCTTATTGTC TGGGGCGATA CCCGCGAACA
    1321 GGCCTTACAG CGCAGCCGTC GCGCCCTGGC GGAATATGTA GTGGAGGGTA TGCCGACCGT
    1381 AATCCCGTTC CACCAGCATA TTGTGGAAAA TCCGGCCTTT GTTGGGAATG ATGAAGGTTT
    1441 TGAAATTTAC ACCAAATGGA TAGAGGAAGT ATGGGACAAT CCTATTGCAC CTTATGTGGA
    1501 TGCGTCCGAA CTGGATGAGG ATGAAGATAA AACGCCAGCG CAGAAAGTTG TTGTAGAAAT
    1561 TAATGGCCGC CGCGTTGAGG TGGCGCTGCC GGGCGATCTG GCACTGGGCG GGACAGCGGG
    1621 ACCGAAAAAA AAGGCTAAAA AACGGAGGGC GGGCGGTGCG AAAGCAGGCG TCAGTGGGGA
    1681 TGCGGTAGCG GCACCGATGC AGGGTACGGT GATTAAAGTA AATGTCGAGG AAGGCGCAGA
    1741 GGTGAACGAA GGTGATACCG TGGTTGTACT TGAAGCAATG AAGATGGAAA ACCCGGTGAA
    1801 AGCCCATAAA TCAGGCACGG TGACGGGTTT GACCGTTGCC GCGGGTGAAG GCGTCAATAA
    1861 AGGTGTGGTT CTGTTAGAGA TTAAATAAcc aggcatcaaa taaaacgaaa ggctcagtcg
    1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
    1981 actggctcac cttcgggtgg gcctttctgc gtttatatag tAGAGACCAG GTGC

//`

sequences.seq_ACC_outro_gblock = {}; sequences.seq_ACC_outro_gblock.varName = "seq_ACC_outro_gblock"; sequences.seq_ACC_outro_gblock.name = "ACC outro gblock"; sequences.seq_ACC_outro_gblock.seqType = "both"; sequences.seq_ACC_outro_gblock.comp = true; sequences.seq_ACC_outro_gblock.data = `LOCUS ACC_outro_gblock 2216 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    2179..2199
                    /label="Suffix"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    388..417
                    /label="Spacer"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc_feature    18..52
                    /label="Promoter"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    2050..2178
                    /label="Terminator"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    418..2049
                    /label="ACCbeta"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    77..90
                    /label="RBS"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    94..387
                    /label="ZFA"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_binding    53..76
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 CCAGCCGGTC TCATAGTttg acggctagct cagtcctagg tatagtgcta gcTGGAATTG
      61 TGAGCGGATA ACAATTGAAA GAGGTGACAT ATGACATCTG CAGCGGCCCC GGGGGAACGG
     121 CCATTCCAGT GTCGGATTTG CATGAGAAAC TTTAGCGATT CACCGACCCT GCGTCGTCAC
     181 ACCCGCACGC ATACTGGCGA AAAGCCTTTC CAGTGTCGCA TTTGCATGCG CAATTTTTCC
     241 GTTCGTCATA ATTTAACGCG CCACCTTCGC ACGCACACCG GTGAGAAACC GTTCCAATGC
     301 CGTATCTGTA TGCGTAACTT TTCGGACCGC ACTAGTCTGG CGCGACATTT GAAAACCCAT
     361 TATCCCTATG ATGTGCCGGA TTACGCTggt ggaagtggag gtggaagtgg aggtagtATG
     421 ACGATCAGTT CCCCGCTGAT CGATGTTGCT AACCTGCCGG ATATTAACAC CACGGCGGGA
     481 AAAATAGCTG ATCTCAAAGC GCGCCGCGCG GAAGCCCATT TCCCTATGGG CGAAAAGGCG
     541 GTTGAGAAAG TGCACGCCGC AGGTCGTCTG ACAGCACGGG AACGTCTGGA TTATCTCTTA
     601 GATGAAGGCT CTTTCATCGA AACCGATCAA CTTGCCCGCC ACCGGACGAC GGCGTTTGGC
     661 CTGGGCGCCA AACGTCCGGC AACCGATGGG ATCGTGACCG GGTGGGGTAC CATTGATGGC
     721 CGCGAAGTCT GTATTTTCAG CCAGGATGGA ACTGTTTTTG GCGGCGCGCT GGGCGAGGTT
     781 TACGGGGAAA AAATGATAAA AATTATGGAA TTAGCGATCG ATACGGGGCG TCCGCTGATT
     841 GGCCTGTACG AGGGAGCGGG CGCCCGAATC CAGGATGGTG CAGTCTCGCT AGATTTCATT
     901 AGTCAAACAT TTTACCAGAA TATCCAGGCC AGCGGAGTAA TTCCCCAGAT TAGTGTGATC
     961 ATGGGCGCGT GCGCCGGTGG GAATGCTTAT GGCCCAGCGT TGACAGACTT CGTGGTGATG
    1021 GTCGATAAGA CGTCTAAAAT GTTTGTGACG GGTCCAGATG TGATTAAGAC CGTGACTGGC
    1081 GAGGAAATTA CCCAGGAGGA GCTTGGTGGT GCAACGACAC ACATGGTAAC TGCGGGAAAC
    1141 TCCCATTATA CTGCGGCTAC CGACGAGGAA GCCTTGGATT GGGTTCAAGA TCTGGTTAGC
    1201 TTTCTGCCTT CCAACAACCG GTCTTACACT CCCCTGGAAG ATTTTGACGA AGAAGAAGGT
    1261 GGGGTTGAAG AAAATATCAC CGCAGACGAC CTTAAACTCG ACGAAATCAT TCCGGATTCA
    1321 GCTACCGTAC CATACGACGT TCGTGACGTC ATCGAATGTC TTACCGATGA CGGTGAGTAT
    1381 TTGGAGATTC AGGCGGACCG CGCCGAAAAC GTCGTGATTG CGTTTGGGCG CATTGAAGGT
    1441 CAAAGCGTTG GCTTTGTGGC AAATCAGCCG ACTCAGTTCG CGGGTTGTCT GGATATTGAT
    1501 TCATCGGAAA AAGCAGCACG TTTCGTGCGT ACGTGCGATG CGTTTAACAT CCCGATTGTA
    1561 ATGCTGGTCG ACGTGCCGGG ATTTCTGCCG GGAGCCGGTC AAGAATATGG TGGTATTCTG
    1621 AGACGCGGCG CTAAATTATT ATATGCATAT GGCGAAGCAA CAGTACCGAA AATCACCGTT
    1681 ACCATGCGTA AGGCATACGG GGGTGCGTAC TGCGTCATGG GCTCAAAAGG CTTGGGCAGT
    1741 GACATCAATC TAGCGTGGCC GACCGCTCAG ATTGCCGTGA TGGGTGCCGC GGGCGCAGTC
    1801 GGCTTCATAT ATCGCAAAGA ACTGATGGCG GCCGATGCCA AAGGTCTGGA TACGGTGGCT
    1861 CTGGCTAAAA GCTTTGAGCG CGAGTATGAA GACCACATGC TGAATCCTTA TCATGCTGCC
    1921 GAGCGTGGGC TCATAGACGC GGTAATCTTA CCCTCGGAAA CCAGGGGCCA GATTAGCCGA
    1981 AATCTGCGTC TTCTGAAGCA TAAAAATGTG ACACGCCCAG CCAGAAAACA TGGTAACATG
    2041 CCTTTGTAAc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
    2101 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
    2161 ggcctttctg cgtttatata ctagtagcgg ccgctgcagT GCCAGAGACC CCATTA

//`

sequences.seq_ACS_intro_gblock = {}; sequences.seq_ACS_intro_gblock.varName = "seq_ACS_intro_gblock"; sequences.seq_ACS_intro_gblock.name = "ACS intro gblock"; sequences.seq_ACS_intro_gblock.seqType = "linear"; sequences.seq_ACS_intro_gblock.comp = true; sequences.seq_ACS_intro_gblock.data = `LOCUS ACS_intro_gblock 458 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    18..39
                    /label="Prefix"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    40..74
                    /label="promoter"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    122..415
                    /label="ZFB"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    misc_binding    75..98
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    99..118
                    /label="RBS"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    416..445
                    /label="Spacer"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9

ORIGIN 

       1 GTTTCAGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
      61 ggtacagtgc tagcTGGAAT TGTGAGCGGA TAACAATTTC GACTCGTTCG ATTAAAGAAT
     121 GACCAGTGCG GCTGCCCCTG GTGAACGCCC GTTTCAGTGT CGGATTTGCA TGCGAAATTT
     181 CAGCAAAAAA GATCATCTCC ACCGTCATAC TCGCACACAC ACTGGGGAAA AACCGTTCCA
     241 GTGTCGCATT TGCATGCGTA ACTTTTCCTT GTCACAAACC CTGAAACGTC ACCTTCGCAC
     301 GCATACGGGC GAGAAGCCCT TCCAGTGTAG AATCTGCATG CGCAACTTTT CGCGTTTAGA
     361 TATGCTGGCG CGTCATCTGA AAACCCATTA TCCATACGAT GTGCCGGACT ATGCAggtgg
     421 aagtggaggt ggaagtggag gtagtAGAGA CCGGACTA

//`

sequences.seq_ACS_outro_gblock = {}; sequences.seq_ACS_outro_gblock.varName = "seq_ACS_outro_gblock"; sequences.seq_ACS_outro_gblock.name = "ACS outro gblock"; sequences.seq_ACS_outro_gblock.seqType = "linear"; sequences.seq_ACS_outro_gblock.comp = true; sequences.seq_ACS_outro_gblock.data = `LOCUS ACS_outro_gblock 2143 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    2106..2126
                    /label="Suffix"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    18..1973
                    /label="ACS"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    misc_feature    1977..2105
                    /label="Terminator"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 GTTTCAGGTC TCATAGTATG TCTCAGATTC ACAAACATAC TATCCCTGCA AACATTGCTG
      61 ACCGTTGCCT GATTAATCCT CAACAGTATG AAGCCATGTA CCAGCAGTCG ATTAATGTGC
     121 CGGATACATT TTGGGGCGAA CAGGGGAAGA TTCTGGACTG GATCAAACCG TACCAAAAAG
     181 TGAAAAATAC CAGTTTCGCA CCGGGTAATG TTAGCATCAA ATGGTACGAA GACGGCACTC
     241 TTAACCTTGC GGCGAATTGT CTGGATCGTC ATCTACAAGA AAACGGCGAT CGTACAGCGA
     301 TTATATGGGA GGGTGATGAT GCTAGCCAGT CGAAGCATAT CAGCTATAAA GAACTTCATC
     361 GCGACGTATG CCGCTTCGCA AACACGTTGT TAGAATTAGG CATCAAGAAA GGTGATGTGG
     421 TCGCCATTTA TATGCCAATG GTTCCGGAAG CGGCCGTGGC TATGCTCGCA TGCGCCCGTA
     481 TTGGAGCGGT CCATTCGGTT ATTTTTGGGG GGTTTTCCCC GGAGGCAGTC GCCGGCCGCA
     541 TTATTGATTC CAATAGCCGT CTCGTCATCA CCTCTGATGA AGGAGTTCGG GCGGGCCGCA
     601 GCATTCCTCT GAAAAAGAAT GTAGACGATG CTCTGAAAAA CCCTAACGTC ACGAGTGTCG
     661 AGCACGTTGT TGTGTTAAAA CGCACCGGTG GTAAAATTGA CTGGCAGGAA GGGCGTGACT
     721 TGTGGTGGCA TGACCTTGTA GAACAAGCGT CCGATCAGCA TCAGGCTGAA GAGATGAACG
     781 CAGAGGACCC GCTGTTCATC TTATATACCA GCGGATCTAC AGGTAAACCC AAAGGGGTGC
     841 TGCATACTAC GGGCGGTTAT TTGGTGTACG CCGCCCTGAC TTTTAAATAC GTCTTTGATT
     901 ACCACCCCGG TGACATCTAT TGGTGTACCG CTGATGTCGG TTGGGTTACC GGACATAGCT
     961 ATCTGCTGTA TGGCCCCTTG GCATGTGGGG CCACAACGTT GATGTTTGAA GGAGTGCCGA
    1021 ATTGGCCGAC CCCAGCCCGC ATGGCGCAGG TAGTTGATAA GCATCAGGTG AACATCCTGT
    1081 ACACGGCTCC GACGGCCATT CGAGCTCTGA TGGCGGAAGG CGATAAAGCG ATAGAAGGCA
    1141 CGGATCGCTC TTCTCTCCGC ATCCTGGGTT CAGTTGGCGA GCCAATCAAC CCAGAGGCGT
    1201 GGGAGTGGTA CTGGAAAAAA ATTGGTAATG AAAAATGCCC GGTCGTGGAC ACCTGGTGGC
    1261 AGACCGAAAC GGGAGGCTTT ATGATTACCC CGCTGCCAGG CGCGACGGAG CTAAAGGCAG
    1321 GGAGCGCAAC AAGGCCTTTT TTCGGTGTGC AACCGGCCTT AGTGGACAAT GAGGGTAATC
    1381 CGCTGGAAGG GGCGACCGAG GGATCACTGG TGATTACTGA CAGTTGGCCG GGCCAGGCAC
    1441 GAACCCTGTT CGGTGATCAC GAAAGATTCG AACAAACGTA TTTCTCCACA TTTAAAAACA
    1501 TGTATTTTTC AGGTGACGGC GCGCGTAGAG ATGAAGATGG TTATTATTGG ATCACCGGCC
    1561 GGGTCGATGA TGTGCTCAAC GTTAGCGGTC ACCGTCTTGG CACGGCGGAA ATCGAATCGG
    1621 CTCTGGTGGC CCACCCTAAG ATCGCGGAGG CAGCCGTGGT AGGCATTCCG CATAACATAA
    1681 AAGGCCAGGC CATTTACGCG TATGTGACTT TGAATCACGG CGAAGAACCA TCGCCGGAAT
    1741 TATACGCGGA AGTACGCAAC TGGGTTCGGA AAGAAATCGG TCCACTGGCA ACCCCGGATG
    1801 TACTGCACTG GACCGATTCA CTGCCCAAAA CGCGCAGTGG GAAGATCATG CGTCGCATAC
    1861 TGCGTAAAAT TGCGGCCGGT GATACTTCAA ACCTCGGCGA TACCTCCACC CTTGCAGATC
    1921 CCGGCGTAGT TGAAAAACTG CTGGAAGAGA AACAAGCCAT TGCGATGCCG AGTTGAccag
    1981 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
    2041 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
    2101 ttatatacta gtagcggccg ctgcagTGCC AGAGACCGGA CTA

//`

sequences.seq_BsaI_FWD_primer = {}; sequences.seq_BsaI_FWD_primer.varName = "seq_BsaI_FWD_primer"; sequences.seq_BsaI_FWD_primer.name = "BsaI FWD primer"; sequences.seq_BsaI_FWD_primer.seqType = "linear"; sequences.seq_BsaI_FWD_primer.comp = false; sequences.seq_BsaI_FWD_primer.data = `LOCUS BsaI_FWD_primer 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 CTCTCAGGTC TCATGCCagt ccggcaaaaa agggc

//`

sequences.seq_BsaI_REV_primer = {}; sequences.seq_BsaI_REV_primer.varName = "seq_BsaI_REV_primer"; sequences.seq_BsaI_REV_primer.name = "BsaI REV primer"; sequences.seq_BsaI_REV_primer.seqType = "linear"; sequences.seq_BsaI_REV_primer.comp = false; sequences.seq_BsaI_REV_primer.data = `LOCUS BsaI_REV_primer 40 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ATCTTAGGTC TCTCTCCcag aaatcatcct tagcgaaagc

//`

sequences.seq_CoA_4x_Assembly = {}; sequences.seq_CoA_4x_Assembly.varName = "seq_CoA_4x_Assembly"; sequences.seq_CoA_4x_Assembly.name = "CoA 4x Assembly"; sequences.seq_CoA_4x_Assembly.seqType = "both"; sequences.seq_CoA_4x_Assembly.comp = true; sequences.seq_CoA_4x_Assembly.data = `LOCUS CoA_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2480..2488
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2469..2477
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Zinc Finger Site    2458..2466
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    2447..2455
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2436..2444
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2414..2422
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2403..2411
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    2425..2433
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    HIV-RT Binding Site join(2489..2492,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AA

//`

sequences.seq_CoA_4x_Comp_Assembly = {}; sequences.seq_CoA_4x_Comp_Assembly.varName = "seq_CoA_4x_Comp_Assembly"; sequences.seq_CoA_4x_Comp_Assembly.name = "CoA 4x Comp Assembly"; sequences.seq_CoA_4x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_4x_Comp_Assembly.comp = true; sequences.seq_CoA_4x_Comp_Assembly.data = `LOCUS CoA_4x_Comp_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2425..2433)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2480..2488)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2469..2477)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    complement(2447..2455)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Zinc Finger Site    complement(2414..2422)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2403..2411)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2436..2444)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2392..2400)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    HIV-RT Binding Site join(2489..2492,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2458..2466)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
    2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
    2461 AGCCGCATGG CATCGACATG CCGTCCTCAA AA

//`

sequences.seq_CoA_4x_Full_Part = {}; sequences.seq_CoA_4x_Full_Part.varName = "seq_CoA_4x_Full_Part"; sequences.seq_CoA_4x_Full_Part.name = "CoA 4x Full Part"; sequences.seq_CoA_4x_Full_Part.seqType = "both"; sequences.seq_CoA_4x_Full_Part.comp = true; sequences.seq_CoA_4x_Full_Part.data = `LOCUS CoA_4x_Full_Part 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2779..2787)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2425..2433
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2447..2455
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    HIV-RT Binding Site 2489..2598
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    2436..2444
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    2414..2422
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2746..2754)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Scar                2668..2675
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2458..2466
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site join(2832..2835,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2735..2743)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2768..2776)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2790..2798)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2801..2809)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Promoter            2676..2710
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2812..2820)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2403..2411
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2480..2488
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    gBlock              2545..2669
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    2469..2477
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2757..2765)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          2599..2667
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    misc_binding        2712..2734
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2823..2831)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
    2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
    2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
    2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
    2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTCCCAGC CGCATGGCAT CGACATCCCA
    2761 GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
    2821 ATGCCGTCCT CAAAA

//`

sequences.seq_CoA_4x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.varName = "seq_CoA_4x_r_oligo_Complement_Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.name = "CoA 4x r_oligo Complement Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.comp = true; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.data = `LOCUS CoA_4x_r_oligo_Complemen 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site complement(42..50)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(53..61)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(64..72)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(75..83)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(86..94)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(97..105)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(108..116)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(119..127)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(130..138)
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          complement(42..138)
                    /label="Temp2"
                    /note="sequence: GAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          42..138
                    /label="Temp"
                    /note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTC"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
      61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
     121 CATCGACATG CCGTCCTCAA AACGAGACCA ACCTG

//`

sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.varName = "seq_CoA_4x_r_oligo_Duplex_126145_REV_1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.name = "CoA 4x r_oligo Duplex 126-145 REV 1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.comp = false; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.data = `LOCUS CoA_4x_r_oligo_Duplex_126 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCGTTTTCCC AGCCGCATGG

//`

sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.varName = "seq_CoA_4x_r_oligo_Duplex_6988_FWD_1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.name = "CoA 4x r_oligo Duplex 69-88 FWD 1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.comp = false; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.data = `LOCUS CoA_4x_r_oligo_Duplex_69- 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TGGGATGTCG ATGCCATGCG

//`

sequences.seq_CoA_4x_r_oligo_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Duplex.varName = "seq_CoA_4x_r_oligo_Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.name = "CoA 4x r_oligo Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex.comp = true; sequences.seq_CoA_4x_r_oligo_Duplex.data = `LOCUS CoA_4x_r_oligo_Duplex 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site 42..50
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site 53..61
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 64..72
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 75..83
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 86..94
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 97..105
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 108..116
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 119..127
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 130..138
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          complement(126..145)
                    /label="CoA 4x r_oligo Duplex 126-145 REV 1"
                    /note="sequence: TCGTTTTCCCAGCCGCATGG"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          18..138
                    /label="Temp Fwd"
                    /note="sequence: TGGAATTGTGAGCGGATAACAATTGAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          complement(20..140)
                    /label="Temp Rev"
                    /note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTCAATTGTTATCCGCTCACAATTCCA"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    primer          69..88
                    /label="CoA 4x r_oligo Duplex 69-88 FWD 1"
                    /note="sequence: TGGGATGTCGATGCCATGCG"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
      61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
     121 CGATGCCATG CGGCTGGGAA AACGAGACCA ACCTG

//`

sequences.seq_CoA_6x_Assembly = {}; sequences.seq_CoA_6x_Assembly.varName = "seq_CoA_6x_Assembly"; sequences.seq_CoA_6x_Assembly.name = "CoA 6x Assembly"; sequences.seq_CoA_6x_Assembly.seqType = "both"; sequences.seq_CoA_6x_Assembly.comp = true; sequences.seq_CoA_6x_Assembly.data = `LOCUS CoA_6x_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2502..2510
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    2491..2499
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2480..2488
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2524..2532
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2469..2477
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2458..2466
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2513..2521
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2447..2455
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2436..2444
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2425..2433
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2414..2422
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2403..2411
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    HIV-RT Binding Site join(2533..2536,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
    2521 CATGCGGCTG GGAAAA

//`

sequences.seq_CoA_6x_Comp_Assembly = {}; sequences.seq_CoA_6x_Comp_Assembly.varName = "seq_CoA_6x_Comp_Assembly"; sequences.seq_CoA_6x_Comp_Assembly.name = "CoA 6x Comp Assembly"; sequences.seq_CoA_6x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_6x_Comp_Assembly.comp = true; sequences.seq_CoA_6x_Comp_Assembly.data = `LOCUS CoA_6x_Comp_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    complement(2491..2499)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2502..2510)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2480..2488)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2458..2466)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2447..2455)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    complement(2436..2444)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2425..2433)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2414..2422)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2403..2411)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2392..2400)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2513..2521)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2524..2532)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    HIV-RT Binding Site join(2533..2536,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    complement(2469..2477)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
    2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
    2461 AGCCGCATGG CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA
    2521 CATGCCGTCC TCAAAA

//`

sequences.seq_CoA_6x_Full_Part = {}; sequences.seq_CoA_6x_Full_Part.varName = "seq_CoA_6x_Full_Part"; sequences.seq_CoA_6x_Full_Part.name = "CoA 6x Full Part"; sequences.seq_CoA_6x_Full_Part.seqType = "both"; sequences.seq_CoA_6x_Full_Part.comp = true; sequences.seq_CoA_6x_Full_Part.data = `LOCUS CoA_6x_Full_Part 2923 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    Zinc Finger Site    2425..2433
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_binding        2756..2778
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2790..2798)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2469..2477
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2900..2908)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2480..2488
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2447..2455
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2867..2875)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site 2533..2642
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2524..2532
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    complement(2823..2831)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2458..2466
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2812..2820)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2436..2444
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    complement(2878..2886)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    2491..2499
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2856..2864)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2502..2510
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Terminator          2643..2711
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2834..2842)
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2513..2521
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    complement(2779..2787)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Zinc Finger Site    2403..2411
                        /label="ZFa Site"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Promoter            2720..2754
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2845..2853)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Scar                2712..2719
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2414..2422
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    gBlock              2589..2713
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2889..2897)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2801..2809)
                        /label="ZFb Site"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site join(2920..2923,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2911..2919)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
    2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
    2521 CATGCGGCTG GGAAAAAAAA CGTGGCGCCC GAACAGGGAC GGATAGCTCA GTCGGTAGAG
    2581 CATCAGACTT TTAATCTGAG GGTCCAGGGT TCAAGTCCCT GTTCGGGCGC CACGTTTTTT
    2641 TTattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact tcccttgggg
    2701 ttatcattgg gtactagagT TGACGGCTAG CTCAGTCCTA GGTACAGTGC TAGCTGGAAT
    2761 TGTGAGCGGA TAACAATTCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
    2821 ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT CGACATCCCA GCCGCATGGC
    2881 ATCGACATCC CAGCCGCATG GCATCGACAT GCCGTCCTCA AAA

//`

sequences.seq_CoA_6x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.varName = "seq_CoA_6x_r_oligo_Complement_Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.name = "CoA 6x r_oligo Complement Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.comp = true; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.data = `LOCUS CoA_6x_r_oligo_Complemen 199 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site complement(42..50)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(53..61)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(64..72)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(75..83)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(86..94)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(97..105)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(108..116)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(119..127)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(130..138)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(141..149)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(152..160)
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site complement(163..171)
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site complement(174..182)
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
      61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
     121 CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA CATGCCGTCC
     181 TCAAAACGAG ACCAACCTG

//`

sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.varName = "seq_CoA_6x_r_oligo_Duplex_113132_FWD_1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.name = "CoA 6x r_oligo Duplex 113-132 FWD 1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.comp = false; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.data = `LOCUS CoA_6x_r_oligo_Duplex_113 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TGGGATGTCG ATGCCATGCG

//`

sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.varName = "seq_CoA_6x_r_oligo_Duplex_170189_REV_1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.name = "CoA 6x r_oligo Duplex 170-189 REV 1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.comp = false; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.data = `LOCUS CoA_6x_r_oligo_Duplex_170 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCGTTTTCCC AGCCGCATGG

//`

sequences.seq_CoA_6x_r_oligo_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Duplex.varName = "seq_CoA_6x_r_oligo_Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.name = "CoA 6x r_oligo Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex.comp = true; sequences.seq_CoA_6x_r_oligo_Duplex.data = `LOCUS CoA_6x_r_oligo_Duplex 199 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site 42..50
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site 53..61
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 64..72
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 75..83
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 86..94
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 97..105
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 108..116
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 119..127
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 130..138
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 141..149
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 152..160
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site 163..171
                    /label="ZFa Site"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site 174..182
                    /label="ZFb Site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          113..132
                    /label="CoA 6x r_oligo Duplex 113-132 FWD 1"
                    /note="sequence: TGGGATGTCGATGCCATGCG"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          complement(170..189)
                    /label="CoA 6x r_oligo Duplex 170-189 REV 1"
                    /note="sequence: TCGTTTTCCCAGCCGCATGG"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
      61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
     121 CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG
     181 GGAAAACGAG ACCAACCTG

//`

sequences.seq_gblock_1 = {}; sequences.seq_gblock_1.varName = "seq_gblock_1"; sequences.seq_gblock_1.name = "g-block 1"; sequences.seq_gblock_1.seqType = "linear"; sequences.seq_gblock_1.comp = true; sequences.seq_gblock_1.data = `LOCUS g-block_1 300 bp ds-DNA linear 07-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    164..213
                    /label="sTRSV HHRz"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    Biobricking     68..89
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_binding    126..148
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    90..124
                    /label="BBa_J23109'"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    RBS             222..233
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61

ORIGIN 

       1 TGTTGATTGG TGGCACATAA GTAATACCAT GGTCCCTGAA ATTCGGCTCA ACGGTCCGTC
      61 TCATCTGGAA TTCGCGGCCG CTTCTAGAGt ttacagctag ctcagtccta gggactgtgc
     121 tagcTGGAAT TGTGAGCGGA TAACAATTct atggactatg tttCTGTCAC CGGATGTGCT
     181 TTCCGGTCTG ATGAGTCCGT GAGGACGAAA CAGtaccagt gaaagaggag aaaGGATTGA
     241 GACGGTAACA GTTACTTCGA GCGTAATGTC TCAAATGGCG TAGAACGGCA ATGACTGTTc

//`

sequences.seq_gblock_3 = {}; sequences.seq_gblock_3.varName = "seq_gblock_3"; sequences.seq_gblock_3.name = "g-block 3"; sequences.seq_gblock_3.seqType = "linear"; sequences.seq_gblock_3.comp = true; sequences.seq_gblock_3.data = `LOCUS g-block_3 300 bp ds-DNA linear 07-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    Biobricking     199..219
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    terminator      112..198
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          complement(177..198)
                    /label="pMLRT REV"
                    /note="sequence: CTCACGCGTCTCAGCTACTGCAGCGGCCGCTACTAGTAatttgtcctactcaggagagcg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(177..198)
                    /label="pMLRT REV"
                    /note="sequence: CTCACGCGTCTCAGCTAatttgtcctactcaggagagcg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(170..198)
                    /label="pMLRT REV"
                    /note="sequence: atttgtcctactcaggagagcgttcaccg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 GGGAGGTGGA ATAGTTTCGA ACGATGGTTA TTAATCGTAA TAACGGAACG CTGTCTGGAG
      61 GATGATTCTC ACGTCTCAAG GGccttcagg catgcaagcc tctggaggca tcaaataaaa
     121 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     181 ctcctgagta ggacaaatTA CTAGTAGCGG CCGCTGCAGA GTCAGAGACG GTAACATCGA
     241 AGGGGAACTA TTTCGTGGGG CGAGCCCACA CCGTCTCTTC TGCGGAAGAC TTAACACGTT

//`

sequences.seq_gBlock_A = {}; sequences.seq_gBlock_A.varName = "seq_gBlock_A"; sequences.seq_gBlock_A.name = "gBlock A"; sequences.seq_gBlock_A.seqType = "linear"; sequences.seq_gBlock_A.comp = true; sequences.seq_gBlock_A.data = `LOCUS gBlock_A 995 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    863..870
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    785..790
                    /label="double stop codon"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    18..979
                    /label="gBlock A"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    stem_loop       883..905
                    /label="stem loop"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    110..815
                    /label="RFP"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    82..89
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    97..101
                    /label="strong RBS"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    110..815
                    /label="BBa_E1010 (RFP)"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    misc_feature    110..112
                    /label="start codon"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    90..104
                    /label="BBa_B0030 (strong RBS)"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    misc_feature    822..862
                    /label="BBa_B0012"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    misc_feature    822..916
                    /label="BBa_B0014 (double terminator)"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc_feature    871..916
                    /label="BBa_B0011"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    18..39
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    90..916
                    /label="From BBa_K3114015"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    misc_feature    794..799
                    /label="double stop codon"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    misc_feature    40..74
                    /label="BBa_J23105"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 GACTTACGTC TCGtctggaa ttcgcggccg cttctagagt ttacggctag ctcagtccta
      61 ggtactatgc tagcCGAGAC Ctactagaga ttaaagagga gaaatactaa tggcttcctc
     121 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
     181 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
     241 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
     301 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
     361 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
     421 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
     481 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
     541 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
     601 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     661 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     721 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     781 tgcttaataa cgctgatagt gctagtgtag atcgctaata atcacactgg ctcaccttcg
     841 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
     901 ggctttttta ttatttGGTC TCGAAAAAAA ACGTGGCGCC CGAACAGGGA CGGATAGCTC
     961 AGTCGGTAGA GCATCAGACT TTCGAGACGC TTGTG

//`

sequences.seq_gBlock_B = {}; sequences.seq_gBlock_B.varName = "seq_gBlock_B"; sequences.seq_gBlock_B.name = "gBlock B"; sequences.seq_gBlock_B.seqType = "linear"; sequences.seq_gBlock_B.comp = true; sequences.seq_gBlock_B.data = `LOCUS gBlock_B 175 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    Biobricking     138..158
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    15..158
                    /label="gBlock B"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc            72..111
                    /label="identical to E. coli MG1655 between tonB and yciA"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    misc_feature    69..137
                    /label="B0054"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    stem_loop       79..110
                    /label="stem loop"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 GATCGTCGTC TCCCTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC GGGCGCCACG
      61 TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc
     121 ttggggttat cattgggtac tagtagcggc cgctgcagag tcCGAGACGT TCCAT

//`

sequences.seq_GFP_final_synthesized_for_pSB1T3_linear = {}; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.varName = "seq_GFP_final_synthesized_for_pSB1T3_linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.name = "GFP final synthesized for pSB1T3 linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.seqType = "linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.comp = true; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.data = `LOCUS GFP_final_synthesized_f 1240 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature         91..405
                         /label="Zif268 + linker"
                         /ApEinfo_revcolor=#84b0dc
                         /ApEinfo_fwdcolor=#84b0dc
    Recognition Sequence 7..12
                         /label="BsaI 5' site"
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
    misc_feature         1..6
                         /label="rando bases"
                         /ApEinfo_revcolor=#b7e6d7
                         /ApEinfo_fwdcolor=#b7e6d7
    misc_feature         1235..1240
                         /label="rando bases"
                         /ApEinfo_revcolor=#d6b295
                         /ApEinfo_fwdcolor=#d6b295
    misc_feature         18..52
                         /label="new promoter"
                         /ApEinfo_revcolor=#faac61
                         /ApEinfo_fwdcolor=#faac61
    misc_feature         406..1122
                         /label="GFP"
                         /ApEinfo_revcolor=#b1ff67
                         /ApEinfo_fwdcolor=#b1ff67
    Recognition Sequence 1229..1234
                         /label="BsaI 3' site"
                         /ApEinfo_revcolor=#f58a5e
                         /ApEinfo_fwdcolor=#f58a5e
    Scar                 1170..1177
                         /label="BioBrick Scar"
                         /ApEinfo_revcolor=#9eafd2
                         /ApEinfo_fwdcolor=#9eafd2
    misc_feature         1129..1223
                         /label="BBa_B0014 Double Terminator"
                         /ApEinfo_revcolor=#9eafd2
                         /ApEinfo_fwdcolor=#9eafd2
    misc_feature         77..90
                         /label="RBS - J61111"
                         /ApEinfo_revcolor=#ff9ccd
                         /ApEinfo_fwdcolor=#ff9ccd
    misc_binding         54..76
                         /label="LacO"
                         /ApEinfo_revcolor=#84b0dc
                         /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 GATACGGGTC TCACGTTttt acggctagct cagtcctagg tatagtgcta gcTGGAATTG
      61 TGAGCGGATA ACAATTGAAA GAGGCGATAC ATGCACCATC ACCATCATCA CCCGGGTGAG
     121 AAGCCATATG CCTGCCCCGT GGAAAGCTGC GATCGTCGTT TCAGTCGCTC TGATGAACTG
     181 ACACGCCACA TCAGAATACA TACCGGGCAG AAACCGTTTC AATGTCGCAT TTGTATGCGC
     241 AACTTTTCGC GCAGCGACCA TTTAACTACG CATATCCGTA CCCACACTGG TGAAAAACCT
     301 TTCGCGTGTG ATATTTGCGG ACGAAAATTT GCACGGTCAG ACGAGCGTAA ACGTCATACC
     361 AAAATTCATA CGGGCGGCGG CAGCGGCGGC GGTAGTGGTG GGTCCcgtaa aggagaagaa
     421 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     481 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     541 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
     601 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
     661 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
     721 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
     781 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
     841 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
     901 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     961 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
    1021 ctttcgaaag atcccaacga aaagAGGgac cacatggtcc ttcttgagtt tgtaacagct
    1081 gctgggatta cacatggcat ggatgaacta tacaaataat aatggctgtc acactggctc
    1141 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
    1201 ttaatccggc ttttttatta tttTGCCaGA GACCAGGCTT

//`

sequences.seq_GFP_Fusion_in_TetR = {}; sequences.seq_GFP_Fusion_in_TetR.varName = "seq_GFP_Fusion_in_TetR"; sequences.seq_GFP_Fusion_in_TetR.name = "GFP Fusion in TetR"; sequences.seq_GFP_Fusion_in_TetR.seqType = "both"; sequences.seq_GFP_Fusion_in_TetR.comp = true; sequences.seq_GFP_Fusion_in_TetR.data = `LOCUS GFP_Fusion_in_TetR 3675 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    2539..2853
                    /label="Zif268 + linker"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    2466..2500
                    /label="new promoter"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    terminator      2394..2437
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2525..2538
                    /label="RBS - J61111"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    primer_bind     complement(103..120)
                    /label="L4440"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    Biobricking     2440..2461
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    3672..3675
                    /label="3' overhang"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Scar            3618..3625
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      22..79
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    3577..3671
                    /label="BBa_B0014 Double Terminator"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    Biobricking     1..21
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    2854..3570
                    /label="GFP"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    misc_feature    1..21
                    /label="BioBrick suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    primer_bind     2372..2390
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    rep_origin      complement(274..862)
                    /label="ori"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    2462..2465
                    /label="5' overhang"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer_bind     complement(354..373)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    CDS             1128..2312
                    /label="TetR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2440..2461
                    /label="BioBrick prefix"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer          complement(2444..2461)
                    /label="Copy of pSB1T3 REV"
                    /note="sequence: AAGGTCGGTCTCCCTCTctctagaagcggccgcga"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          1..18
                    /label="Copy of pSB1T3 FWD"
                    /note="sequence: AAGGTCGGTCTCCTACTtactagtagcggccgctg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
      61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
     121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    1021 ttggtctgac agctcgagat tctcatgttt gacagcttat catcgataag ctttaatgcg
    1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
    1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
    1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
    1261 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
    1321 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
    1381 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
    1441 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
    1501 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
    1561 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
    1621 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
    1681 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
    1741 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
    1801 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
    1861 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
    1921 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
    1981 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
    2041 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
    2101 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
    2161 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
    2221 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
    2281 gtcgcggtgc atggagccgg gccacctcga cctaactcga gtgccacctg acgtctaaga
    2341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
    2401 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
    2461 gAGAGtttac ggctagctca gtcctaggta tagtgctagc TGGAATTGTG AGCGGATAAC
    2521 AATTGAAAGA GGCGATACAT GCACCATCAC CATCATCACC CGGGTGAGAA GCCATATGCC
    2581 TGCCCCGTGG AAAGCTGCGA TCGTCGTTTC AGTCGCTCTG ATGAACTGAC ACGCCACATC
    2641 AGAATACATA CCGGGCAGAA ACCGTTTCAA TGTCGCATTT GTATGCGCAA CTTTTCGCGC
    2701 AGCGACCATT TAACTACGCA TATCCGTACC CACACTGGTG AAAAACCTTT CGCGTGTGAT
    2761 ATTTGCGGAC GAAAATTTGC ACGGTCAGAC GAGCGTAAAC GTCATACCAA AATTCATACG
    2821 GGCGGCGGCA GCGGCGGCGG TAGTGGTGGG TCCcgtaaag gagaagaact tttcactgga
    2881 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
    2941 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
    3001 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
    3061 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
    3121 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
    3181 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
    3241 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
    3301 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
    3361 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
    3421 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
    3481 cccaacgaaa agAGGgacca catggtcctt cttgagtttg taacagctgc tgggattaca
    3541 catggcatgg atgaactata caaataataa tggctgtcac actggctcac cttcgggtgg
    3601 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt
    3661 ttttattatt tTACT

//`

sequences.seq_GGE114 = {}; sequences.seq_GGE114.varName = "seq_GGE114"; sequences.seq_GGE114.name = "GGE114"; sequences.seq_GGE114.seqType = "both"; sequences.seq_GGE114.comp = true; sequences.seq_GGE114.data = `LOCUS (Addgene_#120731_)_GGE1 5268 bp ds-DNA circular 06-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    terminator      4070..4113
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2002..4115
                    /label="pSB1A3 Region"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    protein_bind    4452..4485
                    /label="loxP"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    source          1..5268
                    /label="source"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer_bind     3638..3657
                    /label="Amp-R"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    promoter        complement(3876..3980)
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    CDS             722..1399
                    /label="mRFP1"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    CDS             complement(3015..3875)
                    /label="AmpR"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    rep_origin      complement(2256..2844)
                    /label="ori"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    promoter        632..662
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer_bind     complement(2085..2102)
                    /label="L4440"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    terminator      2004..2061
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    protein_bind    670..686
                    /label="lac operator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    protein_bind    596..617
                    /label="CAP binding site"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    terminator      1444..1515
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer_bind     4048..4066
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer_bind     complement(2336..2355)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    misc_feature    3156..3156
                    /label="Point Mutation"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      1531..1558
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    RBS             704..715
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    primer          2002..2019
                    /label="Linear pSB1A3 BsmBI Fwd"
                    /note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          complement(4088..4115)
                    /label="BBa_Prefix & Promoter SDM Rev"
                    /note="sequence: cagaaatcatccttagcgaaagctaagg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(4093..4115)
                    /label="BsaI pSB1A3 Rev"
                    /note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          4116..4135
                    /label="BBa_Prefix & Promoter SDM Fwd"
                    /note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          2002..2019
                    /label="BsaI pSB1A3 Fwd"
                    /note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          complement(4093..4115)
                    /label="Linear pSB1A3 BsmBI Rev"
                    /note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          complement(4093..4115)
                    /label="pSB1A3 SapI rev"
                    /note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          2002..2019
                    /label="pSB1A3 fwd SapI"
                    /note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          2002..2023
                    /label="Linear pSB1A3 BsmBI Fwd"
                    /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295

ORIGIN 

       1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
      61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
     121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
     181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
     241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
     301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
     361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
     421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
     481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
     721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
    1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
    1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
    1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
    1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
    1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
    1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
    1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
    1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
    1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
    1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
    1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
    1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
    1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
    1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
    1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
    1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
    1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
    2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
    2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
    2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
    3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
    3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
    4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
    4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
    4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
    4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
    4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
    4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
    4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
    4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
    4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
    4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
    4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
    4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
    4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
    4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
    4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
    4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
    5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
    5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
    5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
    5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa

//`

sequences.seq_Linear_pSB1A3_BsmBI_Fwd = {}; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.varName = "seq_Linear_pSB1A3_BsmBI_Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.name = "Linear pSB1A3 BsmBI Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.comp = false; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.data = `LOCUS Linear_pSB1A3_BsmBI_Fwd 31 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 CTATTTCGTC TCCagtccgg caaaaaaggg c

//`

sequences.seq_Linear_pSB1A3_BsmBI_Rev = {}; sequences.seq_Linear_pSB1A3_BsmBI_Rev.varName = "seq_Linear_pSB1A3_BsmBI_Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.name = "Linear pSB1A3 BsmBI Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.comp = false; sequences.seq_Linear_pSB1A3_BsmBI_Rev.data = `LOCUS Linear_pSB1A3_BsmBI_Rev 36 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ATTCCCCGTC TCCcagaaat catccttagc gaaagc

//`

sequences.seq_Linear_pSB1A3_with_BsmBI_Sites = {}; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.varName = "seq_Linear_pSB1A3_with_BsmBI_Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.name = "Linear pSB1A3 with BsmBI Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.seqType = "linear"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.comp = true; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.data = `LOCUS Linearized_pSB1A3_w/_Bs 2140 bp ds-DNA linear 24-JUN-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    CDS             complement(1027..1887)
                    /label="AmpR"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer_bind     1650..1669
                    /label="Amp-R"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    primer_bind     complement(97..114)
                    /label="L4440"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer_bind     2060..2078
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer_bind     complement(348..367)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    misc_feature    14..2127
                    /label="pSB1A3 Region"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    misc_feature    1168..1168
                    /label="Point Mutation"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      2082..2125
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    terminator      16..73
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    promoter        complement(1888..1992)
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    rep_origin      complement(268..856)
                    /label="ori"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(2100..2127)
                    /label="BBa_Prefix & Promoter SDM Rev"
                    /note="sequence: cagaaatcatccttagcgaaagctaagg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          14..35
                    /label="Linear pSB1A3 BsmBI Fwd"
                    /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295

ORIGIN 

       1 CTATTTCGTC TCCagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
      61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
     121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     241 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    1141 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc
    1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    1741 gagatccagt tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt
    1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    1981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
    2041 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
    2101 cttagctttc gctaaggatg atttctgGGA GACGGGGAAT

//`

sequences.seq_pAC4CL1_FWD = {}; sequences.seq_pAC4CL1_FWD.varName = "seq_pAC4CL1_FWD"; sequences.seq_pAC4CL1_FWD.name = "pAC-4CL1 FWD"; sequences.seq_pAC4CL1_FWD.seqType = "linear"; sequences.seq_pAC4CL1_FWD.comp = false; sequences.seq_pAC4CL1_FWD.data = `LOCUS pAC-4CL1_FWD 39 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TTCCCAGCTC TTCCagtatg gaggaggatt acaaaatgg

//`

sequences.seq_pAC4CL1_REV = {}; sequences.seq_pAC4CL1_REV.varName = "seq_pAC4CL1_REV"; sequences.seq_pAC4CL1_REV.name = "pAC-4CL1 REV"; sequences.seq_pAC4CL1_REV.seqType = "linear"; sequences.seq_pAC4CL1_REV.comp = false; sequences.seq_pAC4CL1_REV.data = `LOCUS pAC-4CL1_REV 39 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TTCCCAGCTC TTCCcaatca caatccattt gctagtttt

//`

sequences.seq_pAC4CL1 = {}; sequences.seq_pAC4CL1.varName = "seq_pAC4CL1"; sequences.seq_pAC4CL1.name = "pAC-4CL1"; sequences.seq_pAC4CL1.seqType = "both"; sequences.seq_pAC4CL1.comp = true; sequences.seq_pAC4CL1.data = `LOCUS pAC-4CL1 6156 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "marker:CmR" "ref:pmid\:16551366" FEATURES Location/Qualifiers 

    CDS             complement(join(5716..6156,1..219))
                    /label="CmR"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    promoter        1969..1975
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    protein_bind    1909..1930
                    /label="CAP binding site"
                    /ApEinfo_revcolor=#31849b
                    /ApEinfo_fwdcolor=#31849b
    CDS             complement(join(5719..6156,1..219))
                    /label="CmR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    promoter        1504..1509
                    /label="tet promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    rep_origin      complement(848..1392)
                    /label="p15A ori"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    promoter        1951..1968
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    2016..3719
                    /label="4CL"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    promoter        1527..1532
                    /label="tet promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    promoter        1945..1950
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    promoter        complement(220..322)
                    /label="cat promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    promoter        1510..1526
                    /label="tet promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    promoter        1945..1975
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3

ORIGIN 

       1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
      61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
     601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
     661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
     721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
     781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
     841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
     961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
    1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
    1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
    1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
    1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
    1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
    1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
    1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttcaagat ttcagtgcaa
    1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
    1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
    1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
    1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
    1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
    1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
    1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
    1861 cgtcctgtgg atccccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
    1921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
    1981 aattgtgagc gtctagagcg cccggggaat tccccatgga ggaggattac aaaatggcgc
    2041 cacaagaaca agcagtttct caggtgatgg agaaacagag caacaacaac aacagtgacg
    2101 tcattttccg atcaaagtta ccggatattt acatcccgaa ccacctatct ctccacgact
    2161 acatcttcca aaacatctcc gaattcgcca ctaagccttg cctaatcaac ggaccaaccg
    2221 gccacgtgta cacttactcc gacgtccacg tcatctcccg ccaaatcgcc gccaattttc
    2281 acaaactcgg cgttaaccaa aacgacgtcg tcatgctcct cctcccaaac tgtcccgaat
    2341 tcgtcctctc tttcctcgcc gcctccttcc gcggcgcaac cgccaccgcc gcaaaccctt
    2401 tcttcactcc ggcggagata gctaaacaag ccaaagcctc caacaccaaa ctcataatca
    2461 ccgaagctcg ttacgtcgac aaaatcaaac cacttcaaaa cgacgacgga gtagtcatcg
    2521 tctgcatcga cgacaacgaa tccgtgccaa tccctgaagg ctgcctccgc ttcaccgagt
    2581 tgactcagtc gacaaccgag gcatcagaag tcatcgactc ggtggagatt tcaccggacg
    2641 acgtggtggc actaccttac tcctctggca cgacgggatt accaaaagga gtgatgctga
    2701 ctcacaaggg actagtcacg agcgttgctc agcaagtcga cggcgagaac ccgaatcttt
    2761 atttccacag cgatgacgtc atactctgtg ttttgcccat gtttcatatc tacgctttga
    2821 actcgatcat gttgtgtggt cttagagttg gtgcggcgat tctgataatg ccgaagtttg
    2881 agatcaatct gctattggag ctgatccaga ggtgtaaagt gacggtggct ccgatggttc
    2941 cgccgattgt gttggccatt gcgaagtctt cggagacgga gaagtatgat ttgagctcga
    3001 taagagtggt gaaatctggt gctgctcctc ttggtaaaga acttgaagat gccgttaatg
    3061 ccaagtttcc taatgccaaa ctcggtcagg gatacggaat gacggaagca ggtccagtgc
    3121 tagcaatgtc gttaggtttt gcaaaggaac cttttccggt taagtcagga gcttgtggta
    3181 ctgttgtaag aaatgctgag atgaaaatag ttgatccaga caccggagat tctctttcga
    3241 ggaatcaacc cggtgagatt tgtattcgtg gtcaccagat catgaaaggt tacctcaaca
    3301 atccggcagc tacagcagag accattgata aagacggttg gcttcatact ggagatattg
    3361 gattgatcga tgacgatgac gagcttttca tcgttgatcg attgaaagaa cttatcaagt
    3421 ataaaggttt tcaggtagct ccggctgagc tagaggcttt gctcatcggt catcctgaca
    3481 ttactgatgt tgctgttgtc gcaatgaaag aagaagcagc tggtgaagtt cctgttgcat
    3541 ttgtggtgaa atcgaaggat tcggagttat cagaagatga tgtgaagcaa ttcgtgtcga
    3601 aacaggttgt gttttacaag agaatcaaca aagtgttctt cactgaatcc attcctaaag
    3661 ctccatcagg gaagatattg aggaaagatc tgagggcaaa actagcaaat ggattgtgac
    3721 catgggcggc cgctgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
    3781 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
    3841 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
    3901 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
    3961 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
    4021 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
    4081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
    4141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
    4201 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
    4261 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
    4321 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
    4381 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
    4441 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
    4501 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
    4561 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
    4621 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
    4681 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
    4741 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
    4801 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
    4861 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
    4921 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
    4981 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
    5041 gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga
    5101 taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt
    5161 acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt
    5221 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc
    5281 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac
    5341 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
    5401 tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata
    5461 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
    5521 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
    5581 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
    5641 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
    5701 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
    5761 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
    5821 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
    5881 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
    5941 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
    6001 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
    6061 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
    6121 ccatatcacc agctcaccgt ctttcattgc catacg

//`

sequences.seq_pduABJKNUT_Fwd = {}; sequences.seq_pduABJKNUT_Fwd.varName = "seq_pduABJKNUT_Fwd"; sequences.seq_pduABJKNUT_Fwd.name = "pduABJKNUT Fwd"; sequences.seq_pduABJKNUT_Fwd.seqType = "linear"; sequences.seq_pduABJKNUT_Fwd.comp = false; sequences.seq_pduABJKNUT_Fwd.data = `LOCUS pduABJKNUT_Fwd 46 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCTGCTCGTC TCTgaccaca acggtttccc tgtagaaata attttg

//`

sequences.seq_pduABJKNUT_promoter = {}; sequences.seq_pduABJKNUT_promoter.varName = "seq_pduABJKNUT_promoter"; sequences.seq_pduABJKNUT_promoter.name = "pduABJKNUT promoter"; sequences.seq_pduABJKNUT_promoter.seqType = "linear"; sequences.seq_pduABJKNUT_promoter.comp = true; sequences.seq_pduABJKNUT_promoter.data = `LOCUS *pduABJKNUT_promoter 133 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    38..72
                    /label="BBa_J23114"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    34..37
                    /label="Overhang pSB1C3"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    97..100
                    /label="Overhang pduABJKNUT"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    misc_binding    74..96
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 AAGTGTTCGA ACGCTTCAGA TCTGTACGTC TCACAACttt atggctagct cagtcctagg
      61 tacaatgcta gcTGGAATTG TGAGCGGATA ACAATTGACC AGAGACGTCT GCTTGCCAAC
     121 CGTAGAACTT ACG

//`

sequences.seq_pduABJKNUT_Rev = {}; sequences.seq_pduABJKNUT_Rev.varName = "seq_pduABJKNUT_Rev"; sequences.seq_pduABJKNUT_Rev.name = "pduABJKNUT Rev"; sequences.seq_pduABJKNUT_Rev.seqType = "linear"; sequences.seq_pduABJKNUT_Rev.comp = false; sequences.seq_pduABJKNUT_Rev.data = `LOCUS pduABJKNUT_Rev 42 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ACTGTTCGTC TCAgctttgt tagcagccgg atccaaaagt ag

//`

sequences.seq_PduD_final_product = {}; sequences.seq_PduD_final_product.varName = "seq_PduD_final_product"; sequences.seq_PduD_final_product.name = "PduD (final product)"; sequences.seq_PduD_final_product.seqType = "linear"; sequences.seq_PduD_final_product.comp = true; sequences.seq_PduD_final_product.data = `LOCUS *PduD_(final_product) 658 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    115..154
                    /label="random bp"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    18..112
                    /label="BBa_B0014 Double Terminator"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    653..658
                    /label="rando runway"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    218..231
                    /label="Anderson RBS 7"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    Scar            59..66
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    583..621
                    /label="BBa_B1006"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    1..6
                    /label="Rando Runway"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    286..582
                    /label="Spacer + ZFc"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    misc_feature    14..17
                    /label="5' overhang with pdu"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    159..193
                    /label="J23101 Anderson Promoter"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    232..285
                    /label="pduD (1-18 aa)"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_binding    195..217
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 TGAGTACGTC TCAAAGCtca cactggctca ccttcgggtg ggcctttctg cgtttatata
      61 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tttgAAGGGA
     121 GTAAGTCTTC GAATGGTTCA GTTATAACTA TATAgctgtt tacagctagc tcagtcctag
     181 gtattatgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGAGACT CATGGAAATC
     241 AACGAAAAAC TGCTGCGTCA GATTATTGAG GACGTGTTAC GCGATACGTC GGCGGCCGCA
     301 CCAGGCGAAC GCCCATTTCA GTGTCGCATC TGCATGCGTA ATTTCTCTTC ACCGAGCAAA
     361 CTTATTCGCC ATACCCGTAC CCATACTGGC GAGAAACCCT TTCAGTGTCG TATCTGTATG
     421 CGAAACTTCT CCGACGGGAG CAACCTGGCT CGGCACTTGC GTACACATAC CGGTGAAAAG
     481 CCGTTTCAAT GCCGTATTTG CATGCGCAAT TTCAGTCGCG TGGATAACTT ACCGAGACAC
     541 CTGAAAACGC ACTACCCGTA TGATGTTCCT GATTATGCGT AAaaaaaaaa accccgcccc
     601 tgacagggcg gggttttttt tAATTAAAGA CACAAGTTTC CGTCAAGAGA CGTCGAGT

//`

sequences.seq_pduD_on_pSB1C3__Final_Part = {}; sequences.seq_pduD_on_pSB1C3__Final_Part.varName = "seq_pduD_on_pSB1C3__Final_Part"; sequences.seq_pduD_on_pSB1C3__Final_Part.name = "pduD on pSB1C3 - Final Part"; sequences.seq_pduD_on_pSB1C3__Final_Part.seqType = "both"; sequences.seq_pduD_on_pSB1C3__Final_Part.comp = true; sequences.seq_pduD_on_pSB1C3__Final_Part.data = `LOCUS pduD_on_pSB1C3_-_Final_ 2589 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    primer_bind     complement(333..352)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    Biobricking     2569..2584
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    2028..2028
                    /label="BioBrick prefix"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    misc_feature    2177..2473
                    /label="Spacer + ZFc"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer_bind     complement(82..99)
                    /label="L4440"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    source          1..2028
                    /label="source"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2050..2084
                    /label="new promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    terminator      1982..2025
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    Scar            2515..2522
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    rep_origin      complement(253..841)
                    /label="ori"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    CDS             complement(1139..1798)
                    /label="CmR"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    join(2585..2589,1..2028)
                    /label="pSB1C3 Backbone Region"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    misc_feature    2585..2589
                    /label="BioBrick suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    terminator      1..58
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    primer_bind     1713..1732
                    /label="CAT-R"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    2123..2176
                    /label="pduD (1-18 aa)"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Biobricking     2029..2049
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    primer_bind     1960..1978
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    misc_feature    2474..2568
                    /label="BBa_B0014 Double Terminator"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    Biobricking     2569..2589
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    terminator      1024..1118
                    /label="lambda t0 terminator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    Biobricking     2028..2049
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    source          2585..2589
                    /label="source"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2109..2122
                    /label="RBS-J61107"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
      61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
    1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
    1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
    1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
    1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
    1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
    1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
    1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
    1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
    1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
    1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
    1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
    1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
    1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
    1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
    1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
    1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
    1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
    2041 cttctagagt ttacagctag ctcagtccta ggtattatgc tagcTGGAAT TGTGAGCGGA
    2101 TAACAATTGA AAGAAGAGAC TCATGGAAAT CAACGAAAAA CTGCTGCGTC AGATTATTGA
    2161 GGACGTGTTA CGCGATACGT CGGCGGCCGC ACCAGGCGAA CGCCCATTTC AGTGTCGCAT
    2221 CTGCATGCGT AATTTCTCTT CACCGAGCAA ACTTATTCGC CATACCCGTA CCCATACTGG
    2281 CGAGAAACCC TTTCAGTGTC GTATCTGTAT GCGAAACTTC TCCGACGGGA GCAACCTGGC
    2341 TCGGCACTTG CGTACACATA CCGGTGAAAA GCCGTTTCAA TGCCGTATTT GCATGCGCAA
    2401 TTTCAGTCGC GTGGATAACT TACCGAGACA CCTGAAAACG CACTACCCGT ATGATGTTCC
    2461 TGATTATGCG TAAtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
    2521 agagagaata taaaaagcca gattattaat ccggcttttt tattatttta ctagtagcgg
    2581 ccgctgcag

//`

sequences.seq_pHIV_Backbone_NEB_FWD = {}; sequences.seq_pHIV_Backbone_NEB_FWD.varName = "seq_pHIV_Backbone_NEB_FWD"; sequences.seq_pHIV_Backbone_NEB_FWD.name = "pHIV Backbone NEB FWD"; sequences.seq_pHIV_Backbone_NEB_FWD.seqType = "linear"; sequences.seq_pHIV_Backbone_NEB_FWD.comp = false; sequences.seq_pHIV_Backbone_NEB_FWD.data = `LOCUS pHIV_Backbone_NEB_FWD 42 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 taaagttctg taatattagt gaaagaggag aaatactaga tg

//`

sequences.seq_pHIV_Backbone_NEB_REV = {}; sequences.seq_pHIV_Backbone_NEB_REV.varName = "seq_pHIV_Backbone_NEB_REV"; sequences.seq_pHIV_Backbone_NEB_REV.name = "pHIV Backbone NEB REV"; sequences.seq_pHIV_Backbone_NEB_REV.seqType = "linear"; sequences.seq_pHIV_Backbone_NEB_REV.comp = false; sequences.seq_pHIV_Backbone_NEB_REV.data = `LOCUS pHIV_Backbone_NEB_REV 22 bp ds-DNA linear 08-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 agtgccagat gaattgcctg ta

//`

sequences.seq_pHIV_GGA_FWD = {}; sequences.seq_pHIV_GGA_FWD.varName = "seq_pHIV_GGA_FWD"; sequences.seq_pHIV_GGA_FWD.name = "pHIV GGA FWD"; sequences.seq_pHIV_GGA_FWD.seqType = "linear"; sequences.seq_pHIV_GGA_FWD.comp = false; sequences.seq_pHIV_GGA_FWD.data = `LOCUS pHIV_GGA_FWD 34 bp ds-DNA linear 07-SEP-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 CTACTACGTC TCTGGAtaga tgccgattag cccg

//`

sequences.seq_pHIV_GGA_REV = {}; sequences.seq_pHIV_GGA_REV.varName = "seq_pHIV_GGA_REV"; sequences.seq_pHIV_GGA_REV.name = "pHIV GGA REV"; sequences.seq_pHIV_GGA_REV.seqType = "linear"; sequences.seq_pHIV_GGA_REV.comp = false; sequences.seq_pHIV_GGA_REV.data = `LOCUS pHIV_GGA_REV 37 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCAGTACGTC TCAgccgctt ctagtatata aacgcag

//`

sequences.seq_pHIV_NEB_FWD = {}; sequences.seq_pHIV_NEB_FWD.varName = "seq_pHIV_NEB_FWD"; sequences.seq_pHIV_NEB_FWD.name = "pHIV NEB FWD"; sequences.seq_pHIV_NEB_FWD.seqType = "linear"; sequences.seq_pHIV_NEB_FWD.comp = false; sequences.seq_pHIV_NEB_FWD.data = `LOCUS pHIV_NEB_FWD 22 bp ds-DNA linear 08-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 caggcaattc atctggcact ac

//`

sequences.seq_pHIV_NEB_REV = {}; sequences.seq_pHIV_NEB_REV.varName = "seq_pHIV_NEB_REV"; sequences.seq_pHIV_NEB_REV.name = "pHIV NEB REV"; sequences.seq_pHIV_NEB_REV.seqType = "linear"; sequences.seq_pHIV_NEB_REV.comp = false; sequences.seq_pHIV_NEB_REV.data = `LOCUS pHIV_NEB_REV 37 bp ds-DNA linear 08-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 tcctctttca ctaatattac agaactttac gaatacc

//`

sequences.seq_pHIV_pTp66p51_Linearized = {}; sequences.seq_pHIV_pTp66p51_Linearized.varName = "seq_pHIV_pTp66p51_Linearized"; sequences.seq_pHIV_pTp66p51_Linearized.name = "pHIV_pTp66p51 Linearized"; sequences.seq_pHIV_pTp66p51_Linearized.seqType = "linear"; sequences.seq_pHIV_pTp66p51_Linearized.comp = true; sequences.seq_pHIV_pTp66p51_Linearized.data = `LOCUS pHIV_pTp66p51_Linearize 3226 bp ds-DNA linear 07-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    1732..3057
                    /label="HIVRT P51"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    terminator      3167..3194
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    Scar            3064..3071
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    20..1705
                    /label="HIVRT p66"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    RBS             1714..1725
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    Scar            3152..3159
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      3080..3151
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    primer          complement(1458..1479)
                    /label="pHIV Backbone NEB REV"
                    /note="sequence: agtgccagatgaattgcctgta"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          1726..1746
                    /label="pHIV GGA FWD"
                    /note="sequence: CTACTACGTCTCTGGAtagatgccgattagcccg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(3179..3194)
                    /label="pHIV GGA REV"
                    /note="sequence: TCAGTACGTCTCAcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(3179..3194)
                    /label="pHIV REV"
                    /note="sequence: AATATCCGTCTCTAGTCcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          5..25
                    /label="pHIV GGA FWD"
                    /note="sequence: CTACTACGTCTCTaaagaggagaaatactagatgccg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          5..34
                    /label="pHIV GGA FWD"
                    /note="sequence: CTACTACGTCTCTGGAtagatgccgattagcccg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          1702..1737
                    /label="pHIV Backbone NEB FWD"
                    /note="sequence: gtaatattagtgaaagaggagaaatactagatgccg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(1685..1721)
                    /label="pHIV NEB REV"
                    /note="sequence: tcctctttcactaatattacagaactttacgaatacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          1460..1481
                    /label="pHIV NEB FWD"
                    /note="sequence: caggcaattcatctggcactac"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(3179..3194)
                    /label="pHIV REV"
                    /note="sequence: ATTACACGTCTCAcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 CTACTACGTC TCTGGATaga tgccgattag cccgattgaa accgttccgg ttaaactgaa
      61 accgggtatg gatggtccga aagttaaaca gtggcctctg accgaagaaa aaatcaaagc
     121 actggttgaa atctgcaccg agatggaaaa agaaggcaaa attagcaaaa tcggtccgga
     181 aaatccgtat aatacaccgg tttttgccat taagaaaaaa gatagcacca aatggcgcaa
     241 actggtggat tttcgtgaac tgaataaacg cacccaggat ttttgggaag ttcagctggg
     301 tattccgcat ccggcaggtc tgaaacagaa aaaaagcgtt accgttctgg atgttggtga
     361 tgcatatttt agcgttccgc tggataaaga tttccgtaaa tataccgcat ttaccatccc
     421 gagcattaat aacgaaacac cgggtattcg ctatcagtat aatgttctgc cgcagggttg
     481 gaaaggtagt ccggcaattt ttcagtgtag catgaccaaa attctggaac cgtttcgtaa
     541 acagaatccg gatattgtga tctaccagta tatggatgat ctgtatgttg gtagcgatct
     601 ggaaattggt cagcatcgta ccaaaattga agaactgcgt cagcatctgc tgcgttgggg
     661 ttttaccaca ccggataaaa aacatcagaa agaaccgcct tttctgtgga tgggttatga
     721 actgcatccg gataaatgga ccgttcagcc gattgttctg ccggaaaaag atagctggac
     781 cgttaatgat attcagaaac tggtgggtaa actgaattgg gcaagccaga tttatgccgg
     841 tattaaagtt cgtcagctgt gtaaactgct gcgtggcacc aaagcactga ccgaagttgt
     901 tccgctgaca gaagaagcag aactggaact ggcagaaaat cgtgaaattc tgaaagaacc
     961 ggttcacggc gtttattatg atccgagcaa agatctgatt gccgaaattc agaaacaggg
    1021 tcagggtcag tggacctatc agatttatca agaaccgttt aaaaacctga aaaccggcaa
    1081 atatgcacgt atgaaaggtg cacataccaa cgatgttaaa cagctgaccg aagcagttca
    1141 gaaaattgca accgaaagca ttgtgatttg gggtaaaacc ccgaaattca aactgccgat
    1201 tcagaaagaa acctgggaag catggtggac cgaatattgg caggcaacct ggattccgga
    1261 atgggaattt gttaataccc ctccgctggt taaactgtgg tatcagctgg aaaaagaacc
    1321 gattattggt gccgaaacct tttatgttga tggtgcagcc aatcgtgaaa ccaaactggg
    1381 taaagcaggt tatgttaccg atcgtggtcg tcagaaagtg gtgccgctga ccgataccac
    1441 caatcagaaa accgaactac aggcaattca tctggcacta caggatagcg gtctggaagt
    1501 taatattgtt accgatagcc agtatgccct gggtattatt caggcacagc cggataaaag
    1561 cgaaagcgaa ctggttagcc agattattga acagctgatc aaaaaagaaa aagtgtacct
    1621 ggcatgggtt ccggcacata aaggtattgg tggtaatgaa caggttgatg gtctggttag
    1681 cgcaggtatt cgtaaagttc tgtaatatta gtgaaagagg agaaatacta gatgccgatt
    1741 agcccgattg aaaccgttcc ggttaaactg aaaccgggta tggatggtcc gaaagttaaa
    1801 cagtggcctc tgaccgaaga aaaaatcaaa gcactggttg aaatctgcac cgagatggaa
    1861 aaagaaggca aaattagcaa aatcggtccg gaaaatccgt ataatacacc ggtttttgcc
    1921 attaagaaaa aagatagcac caaatggcgc aaactggtgg attttcgtga actgaataaa
    1981 cgcacccagg atttttggga agttcagctg ggtattccgc atccggcagg tctgaaacag
    2041 aaaaaaagcg ttaccgttct ggatgttggt gatgcatatt ttagcgttcc gctggataaa
    2101 gatttccgta aatataccgc atttaccatc ccgagcatta ataacgaaac accgggtatt
    2161 cgctatcagt ataatgttct gccgcagggt tggaaaggta gtccggcaat ttttcagtgt
    2221 agcatgacca aaattctgga accgtttcgt aaacagaatc cggatattgt gatctaccag
    2281 tatatggatg atctgtatgt tggtagcgat ctggaaattg gtcagcatcg taccaaaatt
    2341 gaagaactgc gtcagcatct gctgcgttgg ggttttacca caccggataa aaaacatcag
    2401 aaagaaccgc cttttctgtg gatgggttat gaactgcatc cggataaatg gaccgttcag
    2461 ccgattgttc tgccggaaaa agatagctgg accgttaatg atattcagaa actggtgggt
    2521 aaactgaatt gggcaagcca gatttatgcc ggtattaaag ttcgtcagct gtgtaaactg
    2581 ctgcgtggca ccaaagcact gaccgaagtt gttccgctga cagaagaagc agaactggaa
    2641 ctggcagaaa atcgtgaaat tctgaaagaa ccggttcacg gcgtttatta tgatccgagc
    2701 aaagatctga ttgccgaaat tcagaaacag ggtcagggtc agtggaccta tcagatttat
    2761 caagaaccgt ttaaaaacct gaaaaccggc aaatatgcac gtatgaaagg tgcacatacc
    2821 aacgatgtta aacagctgac cgaagcagtt cagaaaattg caaccgaaag cattgtgatt
    2881 tggggtaaaa ccccgaaatt caaactgccg attcagaaag aaacctggga agcatggtgg
    2941 accgaatatt ggcaggcaac ctggattccg gaatgggaat ttgttaatac ccctccgctg
    3001 gttaaactgt ggtatcagct ggaaaaagaa ccgattattg gtgccgaaac cttttaagat
    3061 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
    3121 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
    3181 tgggcctttc tgcgtttata tactagaagc ggcTGAGACG TACTGA

//`

sequences.seq_pHIV_pTp66p51_NEBuilder_Product = {}; sequences.seq_pHIV_pTp66p51_NEBuilder_Product.varName = "seq_pHIV_pTp66p51_NEBuilder_Product"; sequences.seq_pHIV_pTp66p51_NEBuilder_Product.name = "pHIV_pTp66p51 NEBuilder Product"; sequences.seq_pHIV_pTp66p51_NEBuilder_Product.seqType = "both"; sequences.seq_pHIV_pTp66p51_NEBuilder_Product.comp = true; sequences.seq_pHIV_pTp66p51_NEBuilder_Product.data = `LOCUS pHIV_pTp66p51_NEBuilder 6621 bp ds-DNA circular 07-SEP-2020 DEFINITION . KEYWORDS "accession:addgene_78233_145927" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    primer_bind     6305..6324
                    /label="Amp-R"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    Scar            4677..4684
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    promoter        1400..1428
                    /label="tac promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    source          1..6621
                    /label="source"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    CDS             complement(5682..6542)
                    /label="AmpR"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    protein_bind    1434..1453
                    /label="lac operator (symmetric)"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer_bind     complement(4752..4769)
                    /label="L4440"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Scar            4589..4596
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    CDS             complement(183..1265)
                    /label="lacI"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    terminator      91..120
                    /label="T3Te terminator"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    primer_bind     1227..1246
                    /label="LacI-R"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295
    misc_feature    1545..3230
                    /label="HIVRT p66"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    rep_origin      complement(4905..5587)
                    /label="ColE1 origin"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    rep_origin      complement(4923..5511)
                    /label="ori"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    1454..1538
                    /label="on g-block 1"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    RBS             3239..3250
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    terminator      4692..4719
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    misc_feature    3257..4582
                    /label="HIVRT P51"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_RNA        1469..1518
                    /label="sTRSV HHRz"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    terminator      4605..4676
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    promoter        complement(join(6543..6621,1..26))
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    primer_bind     complement(5003..5022)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    RBS             1527..1538
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    CDS             complement(5685..6344)
                    /label="AmpR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer          complement(2983..3004)
                    /label="pHIV Backbone NEB REV"
                    /note="sequence: agtgccagatgaattgcctgta"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(4715..4738)
                    /label="pHIV GGA REV"
                    /note="sequence: TCAGTACGTCTCAgccgcttctagtatataaacgcag"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(3210..3246)
                    /label="pHIV NEB REV"
                    /note="sequence: tcctctttcactaatattacagaactttacgaatacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          1539..1559
                    /label="pHIV GGA FWD"
                    /note="sequence: CTACTACGTCTCTGGAtagatgccgattagcccg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          3218..3259
                    /label="pHIV Backbone NEB FWD"
                    /note="sequence: taaagttctgtaatattagtgaaagaggagaaatactagatg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          1469..1488
                    /label="pHIV FWD"
                    /note="sequence: ATTACACGTCTCActgtcaccggatgtgctttc"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          3227..3262
                    /label="pHIV Backbone NEB FWD"
                    /note="sequence: gtaatattagtgaaagaggagaaatactagatgccg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          1469..1488
                    /label="pHIV FWD"
                    /note="sequence: CGTGAGCGTCTCACGATctgtcaccggatgtgctttc"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(4704..4719)
                    /label="pHIV REV"
                    /note="sequence: AATATCCGTCTCTAGTCcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(4704..4719)
                    /label="pHIV REV"
                    /note="sequence: ATTACACGTCTCAcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          2985..3006
                    /label="pHIV NEB FWD"
                    /note="sequence: caggcaattcatctggcactac"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
      61 gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc
     121 agtgagttga ttgctacgta atgtcggcca attcgcgcta acttacatta attgcgttgc
     181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     241 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     301 gagactggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     361 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     421 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
     481 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     541 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     601 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     661 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     721 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg
     781 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     841 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     901 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     961 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
    1021 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
    1081 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
    1141 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
    1201 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
    1261 ttcatattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
    1321 gttttgcgcc attcgatggc gcgccgcttc gtcaggccac atagctttct tgttctgatc
    1381 ggaacgatcg ttggctgtgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
    1441 agcgctcaca attctatgga ctatgtttct gtcaccggat gtgctttccg gtctgatgag
    1501 tccgtgagga cgaaacagta ctagtgaaag aggagaaata ctagatgccg attagcccga
    1561 ttgaaaccgt tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc
    1621 ctctgaccga agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag
    1681 gcaaaattag caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga
    1741 aaaaagatag caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc
    1801 aggatttttg ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa
    1861 gcgttaccgt tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc
    1921 gtaaatatac cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc
    1981 agtataatgt tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga
    2041 ccaaaattct ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg
    2101 atgatctgta tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac
    2161 tgcgtcagca tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac
    2221 cgccttttct gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg
    2281 ttctgccgga aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga
    2341 attgggcaag ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg
    2401 gcaccaaagc actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag
    2461 aaaatcgtga aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc
    2521 tgattgccga aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac
    2581 cgtttaaaaa cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg
    2641 ttaaacagct gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta
    2701 aaaccccgaa attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat
    2761 attggcaggc aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac
    2821 tgtggtatca gctggaaaaa gaaccgatta ttggtgccga aaccttttat gttgatggtg
    2881 cagccaatcg tgaaaccaaa ctgggtaaag caggttatgt taccgatcgt ggtcgtcaga
    2941 aagtggtgcc gctgaccgat accaccaatc agaaaaccga actacaggca attcatctgg
    3001 cactacagga tagcggtctg gaagttaata ttgttaccga tagccagtat gccctgggta
    3061 ttattcaggc acagccggat aaaagcgaaa gcgaactggt tagccagatt attgaacagc
    3121 tgatcaaaaa agaaaaagtg tacctggcat gggttccggc acataaaggt attggtggta
    3181 atgaacaggt tgatggtctg gttagcgcag gtattcgtaa agttctgtaa tattagtgaa
    3241 agaggagaaa tactagatgc cgattagccc gattgaaacc gttccggtta aactgaaacc
    3301 gggtatggat ggtccgaaag ttaaacagtg gcctctgacc gaagaaaaaa tcaaagcact
    3361 ggttgaaatc tgcaccgaga tggaaaaaga aggcaaaatt agcaaaatcg gtccggaaaa
    3421 tccgtataat acaccggttt ttgccattaa gaaaaaagat agcaccaaat ggcgcaaact
    3481 ggtggatttt cgtgaactga ataaacgcac ccaggatttt tgggaagttc agctgggtat
    3541 tccgcatccg gcaggtctga aacagaaaaa aagcgttacc gttctggatg ttggtgatgc
    3601 atattttagc gttccgctgg ataaagattt ccgtaaatat accgcattta ccatcccgag
    3661 cattaataac gaaacaccgg gtattcgcta tcagtataat gttctgccgc agggttggaa
    3721 aggtagtccg gcaatttttc agtgtagcat gaccaaaatt ctggaaccgt ttcgtaaaca
    3781 gaatccggat attgtgatct accagtatat ggatgatctg tatgttggta gcgatctgga
    3841 aattggtcag catcgtacca aaattgaaga actgcgtcag catctgctgc gttggggttt
    3901 taccacaccg gataaaaaac atcagaaaga accgcctttt ctgtggatgg gttatgaact
    3961 gcatccggat aaatggaccg ttcagccgat tgttctgccg gaaaaagata gctggaccgt
    4021 taatgatatt cagaaactgg tgggtaaact gaattgggca agccagattt atgccggtat
    4081 taaagttcgt cagctgtgta aactgctgcg tggcaccaaa gcactgaccg aagttgttcc
    4141 gctgacagaa gaagcagaac tggaactggc agaaaatcgt gaaattctga aagaaccggt
    4201 tcacggcgtt tattatgatc cgagcaaaga tctgattgcc gaaattcaga aacagggtca
    4261 gggtcagtgg acctatcaga tttatcaaga accgtttaaa aacctgaaaa ccggcaaata
    4321 tgcacgtatg aaaggtgcac ataccaacga tgttaaacag ctgaccgaag cagttcagaa
    4381 aattgcaacc gaaagcattg tgatttgggg taaaaccccg aaattcaaac tgccgattca
    4441 gaaagaaacc tgggaagcat ggtggaccga atattggcag gcaacctgga ttccggaatg
    4501 ggaatttgtt aatacccctc cgctggttaa actgtggtat cagctggaaa aagaaccgat
    4561 tattggtgcc gaaacctttt aagatcgcta ctagagccag gcatcaaata aaacgaaagg
    4621 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
    4681 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaagcggccg
    4741 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    4801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    4861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    4921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    4981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    5041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    5101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    5161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    5221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    5281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    5341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    5401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    5461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    5521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    5581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    5641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    5701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    5761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    5821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    5881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    5941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    6001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    6061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    6121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    6181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    6241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    6301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    6361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    6421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    6481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    6541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    6601 tacatatttg aatgtattta g

//`

sequences.seq_pHIV_pTp66p51 = {}; sequences.seq_pHIV_pTp66p51.varName = "seq_pHIV_pTp66p51"; sequences.seq_pHIV_pTp66p51.name = "pHIV_pTp66p51"; sequences.seq_pHIV_pTp66p51.seqType = "both"; sequences.seq_pHIV_pTp66p51.comp = true; sequences.seq_pHIV_pTp66p51.data = `LOCUS pHIV_pTp66p51 6621 bp ds-DNA circular 09-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_78233_145927" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    primer_bind     complement(5003..5022)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    Scar            4677..4684
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer_bind     1227..1246
                    /label="LacI-R"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295
    RBS             1527..1538
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    source          1..6621
                    /label="source"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    1454..1526
                    /label="on g-block 1"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer_bind     complement(4752..4769)
                    /label="L4440"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    promoter        complement(join(6543..6621,1..26))
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    CDS             complement(5685..6344)
                    /label="AmpR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    terminator      4692..4719
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    misc_feature    3257..4582
                    /label="HIVRT P51"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_RNA        1469..1518
                    /label="sTRSV HHRz"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    RBS             3239..3250
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    CDS             complement(5682..6542)
                    /label="AmpR"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    1545..3230
                    /label="HIVRT p66"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    protein_bind    1434..1453
                    /label="lac operator (symmetric)"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    Scar            4589..4596
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    rep_origin      complement(4923..5511)
                    /label="ori"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    CDS             complement(183..1265)
                    /label="lacI"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    rep_origin      complement(4905..5587)
                    /label="ColE1 origin"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    promoter        1400..1428
                    /label="tac promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    primer_bind     6305..6324
                    /label="Amp-R"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    terminator      91..120
                    /label="T3Te terminator"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    terminator      4605..4676
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    primer          2985..3006
                    /label="pHIV NEB FWD"
                    /note="sequence: caggcaattcatctggcactac"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          1469..1488
                    /label="pHIV FWD"
                    /note="sequence: CGTGAGCGTCTCACGATctgtcaccggatgtgctttc"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          1517..1547
                    /label="pHIV Backbone NEB FWD"
                    /note="sequence: taaagttctgtaatattagtgaaagaggagaaatactagatg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(4704..4719)
                    /label="pHIV REV"
                    /note="sequence: ATTACACGTCTCAcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          3218..3259
                    /label="pHIV Backbone NEB FWD"
                    /note="sequence: taaagttctgtaatattagtgaaagaggagaaatactagatg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(4704..4719)
                    /label="pHIV REV"
                    /note="sequence: AATATCCGTCTCTAGTCcgcagaaaggcccacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(3210..3246)
                    /label="pHIV NEB REV"
                    /note="sequence: tcctctttcactaatattacagaactttacgaatacc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(2983..3004)
                    /label="pHIV Backbone NEB REV"
                    /note="sequence: agtgccagatgaattgcctgta"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          1469..1488
                    /label="pHIV FWD"
                    /note="sequence: ATTACACGTCTCActgtcaccggatgtgctttc"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
      61 gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc
     121 agtgagttga ttgctacgta atgtcggcca attcgcgcta acttacatta attgcgttgc
     181 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     241 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     301 gagactggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     361 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     421 taacatgagc tatcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
     481 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     541 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     601 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     661 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     721 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg
     781 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     841 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     901 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     961 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
    1021 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
    1081 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
    1141 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
    1201 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
    1261 ttcatattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
    1321 gttttgcgcc attcgatggc gcgccgcttc gtcaggccac atagctttct tgttctgatc
    1381 ggaacgatcg ttggctgtgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
    1441 agcgctcaca attctatgga ctatgtttct gtcaccggat gtgctttccg gtctgatgag
    1501 tccgtgagga cgaaacagta ctagtgaaag aggagaaata ctagatgccg attagcccga
    1561 ttgaaaccgt tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc
    1621 ctctgaccga agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag
    1681 gcaaaattag caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga
    1741 aaaaagatag caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc
    1801 aggatttttg ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa
    1861 gcgttaccgt tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc
    1921 gtaaatatac cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc
    1981 agtataatgt tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga
    2041 ccaaaattct ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg
    2101 atgatctgta tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac
    2161 tgcgtcagca tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac
    2221 cgccttttct gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg
    2281 ttctgccgga aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga
    2341 attgggcaag ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg
    2401 gcaccaaagc actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag
    2461 aaaatcgtga aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc
    2521 tgattgccga aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac
    2581 cgtttaaaaa cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg
    2641 ttaaacagct gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta
    2701 aaaccccgaa attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat
    2761 attggcaggc aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac
    2821 tgtggtatca gctggaaaaa gaaccgatta ttggtgccga aaccttttat gttgatggtg
    2881 cagccaatcg tgaaaccaaa ctgggtaaag caggttatgt taccgatcgt ggtcgtcaga
    2941 aagtggtgcc gctgaccgat accaccaatc agaaaaccga actgcaggca attcatctgg
    3001 cactgcagga tagcggtctg gaagttaata ttgttaccga tagccagtat gccctgggta
    3061 ttattcaggc acagccggat aaaagcgaaa gcgaactggt tagccagatt attgaacagc
    3121 tgatcaaaaa agaaaaagtg tacctggcat gggttccggc acataaaggt attggtggta
    3181 atgaacaggt tgatggtctg gttagcgcag gtattcgtaa agttctgtaa tactagtgaa
    3241 agaggagaaa tactagatgc cgattagccc gattgaaacc gttccggtta aactgaaacc
    3301 gggtatggat ggtccgaaag ttaaacagtg gcctctgacc gaagaaaaaa tcaaagcact
    3361 ggttgaaatc tgcaccgaga tggaaaaaga aggcaaaatt agcaaaatcg gtccggaaaa
    3421 tccgtataat acaccggttt ttgccattaa gaaaaaagat agcaccaaat ggcgcaaact
    3481 ggtggatttt cgtgaactga ataaacgcac ccaggatttt tgggaagttc agctgggtat
    3541 tccgcatccg gcaggtctga aacagaaaaa aagcgttacc gttctggatg ttggtgatgc
    3601 atattttagc gttccgctgg ataaagattt ccgtaaatat accgcattta ccatcccgag
    3661 cattaataac gaaacaccgg gtattcgcta tcagtataat gttctgccgc agggttggaa
    3721 aggtagtccg gcaatttttc agtgtagcat gaccaaaatt ctggaaccgt ttcgtaaaca
    3781 gaatccggat attgtgatct accagtatat ggatgatctg tatgttggta gcgatctgga
    3841 aattggtcag catcgtacca aaattgaaga actgcgtcag catctgctgc gttggggttt
    3901 taccacaccg gataaaaaac atcagaaaga accgcctttt ctgtggatgg gttatgaact
    3961 gcatccggat aaatggaccg ttcagccgat tgttctgccg gaaaaagata gctggaccgt
    4021 taatgatatt cagaaactgg tgggtaaact gaattgggca agccagattt atgccggtat
    4081 taaagttcgt cagctgtgta aactgctgcg tggcaccaaa gcactgaccg aagttgttcc
    4141 gctgacagaa gaagcagaac tggaactggc agaaaatcgt gaaattctga aagaaccggt
    4201 tcacggcgtt tattatgatc cgagcaaaga tctgattgcc gaaattcaga aacagggtca
    4261 gggtcagtgg acctatcaga tttatcaaga accgtttaaa aacctgaaaa ccggcaaata
    4321 tgcacgtatg aaaggtgcac ataccaacga tgttaaacag ctgaccgaag cagttcagaa
    4381 aattgcaacc gaaagcattg tgatttgggg taaaaccccg aaattcaaac tgccgattca
    4441 gaaagaaacc tgggaagcat ggtggaccga atattggcag gcaacctgga ttccggaatg
    4501 ggaatttgtt aatacccctc cgctggttaa actgtggtat cagctggaaa aagaaccgat
    4561 tattggtgcc gaaacctttt aagatcgcta ctagagccag gcatcaaata aaacgaaagg
    4621 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
    4681 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaagcggccg
    4741 ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    4801 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    4861 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    4921 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    4981 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    5041 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    5101 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    5161 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    5221 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    5281 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    5341 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    5401 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    5461 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    5521 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    5581 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    5641 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    5701 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    5761 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    5821 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    5881 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    5941 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    6001 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    6061 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    6121 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    6181 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    6241 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    6301 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    6361 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    6421 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    6481 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    6541 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    6601 tacatatttg aatgtattta g

//`

sequences.seq_pMLRT_GGA_FWD = {}; sequences.seq_pMLRT_GGA_FWD.varName = "seq_pMLRT_GGA_FWD"; sequences.seq_pMLRT_GGA_FWD.name = "pMLRT GGA FWD"; sequences.seq_pMLRT_GGA_FWD.seqType = "linear"; sequences.seq_pMLRT_GGA_FWD.comp = false; sequences.seq_pMLRT_GGA_FWD.data = `LOCUS pMLRT_GGA_FWD 29 bp ds-DNA linear 07-SEP-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ATTACACGTC TCTCcaAggc ccgatcgat

//`

sequences.seq_pMLRT_GGA_REV = {}; sequences.seq_pMLRT_GGA_REV.varName = "seq_pMLRT_GGA_REV"; sequences.seq_pMLRT_GGA_REV.name = "pMLRT GGA REV"; sequences.seq_pMLRT_GGA_REV.seqType = "linear"; sequences.seq_pMLRT_GGA_REV.comp = false; sequences.seq_pMLRT_GGA_REV.data = `LOCUS pMLRT_GGA_REV 49 bp ds-DNA linear 08-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCGCCTCGTC TCACCCTtct ggacatttat tatatataaa cgcagaaag

//`

sequences.seq_pMLRT_Linearized = {}; sequences.seq_pMLRT_Linearized.varName = "seq_pMLRT_Linearized"; sequences.seq_pMLRT_Linearized.name = "pMLRT Linearized"; sequences.seq_pMLRT_Linearized.seqType = "linear"; sequences.seq_pMLRT_Linearized.comp = true; sequences.seq_pMLRT_Linearized.data = `LOCUS pMLRT_Linearized 2133 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    terminator      2066..2093
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    terminator      1979..2050
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Scar            1963..1970
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    Scar            2051..2058
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          15..30
                    /label="pMLRT GGA FWD"
                    /note="sequence: ATTACACGTCTCAttggatcccagcaggcc"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(2085..2116)
                    /label="pMLRT GGA REV"
                    /note="sequence: TCGCCTCGTCTCACCCTtctggacatttattatatataaacgcagaaag"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(1367..1387)
                    /label="pMLRT SDM REV"
                    /note="sequence: ggggccagaatgacaagtggc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          1388..1413
                    /label="pMLRT SDM FWD"
                    /note="sequence: ccatgcagtagaggcacttgtcaaac"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 ATTACACGTC TCAttggatc ccagcaggcc tcagggcccg atcgatggga ccaatggggc
      61 agcccctgca agtgttgacc ctaaatatag aagatgagta tcggctacat gagacctcaa
     121 aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
     181 aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
     241 cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
     301 agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
     361 acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
     421 tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
     481 tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
     541 ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
     601 cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
     661 gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
     721 cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
     781 actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
     841 cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
     901 agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
     961 agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
    1021 ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
    1081 attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
    1141 cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
    1201 gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
    1261 tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
    1321 ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca cttgtcattc
    1381 tggcccccca tgcagtagag gcacttgtca aacaaccccc cgaccgctgg ctttccaacg
    1441 cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
    1501 tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
    1561 gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
    1621 cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
    1681 aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
    1741 ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
    1801 gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
    1861 gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
    1921 acgagatctt taaatgacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
    1981 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
    2041 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
    2101 ataataaatg tccagaAGGG TGAGACGAGG CGA

//`

sequences.seq_pMLRT_SDM_FWD = {}; sequences.seq_pMLRT_SDM_FWD.varName = "seq_pMLRT_SDM_FWD"; sequences.seq_pMLRT_SDM_FWD.name = "pMLRT SDM FWD"; sequences.seq_pMLRT_SDM_FWD.seqType = "linear"; sequences.seq_pMLRT_SDM_FWD.comp = false; sequences.seq_pMLRT_SDM_FWD.data = `LOCUS pMLRT_SDM_FWD 21 bp ds-DNA linear 08-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 cccatgcagt agaggcactt g

//`

sequences.seq_pMLRT_SDM_Product = {}; sequences.seq_pMLRT_SDM_Product.varName = "seq_pMLRT_SDM_Product"; sequences.seq_pMLRT_SDM_Product.name = "pMLRT SDM Product"; sequences.seq_pMLRT_SDM_Product.seqType = "both"; sequences.seq_pMLRT_SDM_Product.comp = true; sequences.seq_pMLRT_SDM_Product.data = `LOCUS pMLRT_SDM_Product 5742 bp ds-DNA circular 07-SEP-2020 DEFINITION . KEYWORDS "accession:addgene_78234_146112" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    misc_feature    3339..3450
                    /label="on g-block 2"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    terminator      2157..2251
                    /label="lambda t0 terminator"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295
    CDS             complement(2282..3076)
                    /label="NeoR/KanR"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    terminator      5486..5513
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    CDS             3405..3419
                    /label="enterokinase site"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    RBS             3339..3350
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    CDS             complement(445..1395)
                    /label="Rep101"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    5537..5652
                    /label="on g-block 3"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    terminator      5399..5470
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    CDS             3363..3398
                    /label="6xHN"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    primer_bind     complement(2393..2412)
                    /label="Neo-F"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    CDS             complement(2282..3073)
                    /label="Kan/neoR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    3357..5357
                    /label="MLRT ORF"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer_bind     3003..3022
                    /label="Neo-R"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    rep_origin      1443..1665
                    /label="pSC101 ori"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    primer_bind     3255..3273
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    Scar            5471..5478
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    source          1..5742
                    /label="source"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    terminator      5566..5652
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    Scar            5383..5390
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          complement(5624..5652)
                    /label="pMLRT REV"
                    /note="sequence: atttgtcctactcaggagagcgttcaccg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          4807..4827
                    /label="pMLRT SDM FWD"
                    /note="sequence: cccatgcagtagaggcacttg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          3339..3361
                    /label="pMLRT FWD"
                    /note="sequence: CGTGAGCGTCTCTAAGGaaagaggagaaaggtaccatggg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(5631..5652)
                    /label="pMLRT REV"
                    /note="sequence: CTCACGCGTCTCAGCTACTGCAGCGGCCGCTACTAGTAatttgtcctactcaggagagcg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(5631..5652)
                    /label="pMLRT REV"
                    /note="sequence: CTCACGCGTCTCAGCTAatttgtcctactcaggagagcg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          5653..5672
                    /label="pMLRT FWD"
                    /note="sequence: TACTAGTAGCGGCCGCTGCAGccgccgccctagacctaggg"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer          3451..3466
                    /label="pMLRT GGA FWD"
                    /note="sequence: ATTACACGTCTCTCcaAggcccgatcgat"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          complement(4787..4806)
                    /label="pMLRT SDM REV"
                    /note="sequence: gggccagaatgacaagtggc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(5505..5536)
                    /label="pMLRT GGA REV"
                    /note="sequence: TCGCCTCGTCTCACCCTtctggacatttattatatataaacgcagaaag"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 ttcaggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
      61 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
     121 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
     181 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
     241 tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa
     301 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
     361 tttgatatct aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     421 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     481 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg
     541 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     601 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
     661 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     721 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     781 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     841 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     901 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     961 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
    1021 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
    1081 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
    1141 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
    1201 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
    1261 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
    1321 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
    1381 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
    1441 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
    1501 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
    1561 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
    1621 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
    1681 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
    1741 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
    1801 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
    1861 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
    1921 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
    1981 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
    2041 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
    2101 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
    2161 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
    2221 gaggtcatta ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg
    2281 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
    2341 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
    2401 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
    2461 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
    2521 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
    2581 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
    2641 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
    2701 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
    2761 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
    2821 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
    2881 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
    2941 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
    3001 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
    3061 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
    3121 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
    3181 ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt attatcatga
    3241 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagttgacag
    3301 ctagctcagt cctaggtact gtgctagcgg aattcattaa agaggagaaa ggtaccatgg
    3361 gtcataatca taatcataat cataatcata atcacaacgg tggagatgac gatgacaagg
    3421 gtggtcgaca agcttggatc cctgcaggcc tcagggcccg atcgatggga ccaatggggc
    3481 agcccctgca agtgttgacc ctaaatatag aagatgagta tcggctacat gagacctcaa
    3541 aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
    3601 aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
    3661 cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
    3721 agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
    3781 acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
    3841 tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
    3901 tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
    3961 ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
    4021 cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
    4081 gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
    4141 cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
    4201 actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
    4261 cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
    4321 agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
    4381 agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
    4441 ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
    4501 attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
    4561 cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
    4621 gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
    4681 tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
    4741 ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca cttgtcattc
    4801 tggcccccca tgcagtagag gcacttgtca aacaaccccc cgaccgctgg ctttccaacg
    4861 cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
    4921 tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
    4981 gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
    5041 cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
    5101 aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
    5161 ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
    5221 gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
    5281 gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
    5341 acgagatctt taaatgacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
    5401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
    5461 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
    5521 ataataaatg tccagacctg caggcatgca agcctctaga ggcatcaaat aaaacgaaag
    5581 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
    5641 agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct
    5701 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gc

//`

sequences.seq_pMLRT_SDM_REV = {}; sequences.seq_pMLRT_SDM_REV.varName = "seq_pMLRT_SDM_REV"; sequences.seq_pMLRT_SDM_REV.name = "pMLRT SDM REV"; sequences.seq_pMLRT_SDM_REV.seqType = "linear"; sequences.seq_pMLRT_SDM_REV.comp = false; sequences.seq_pMLRT_SDM_REV.data = `LOCUS pMLRT_SDM_REV 20 bp ds-DNA linear 08-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 gggccagaat gacaagtggc

//`

sequences.seq_pMLRT = {}; sequences.seq_pMLRT.varName = "seq_pMLRT"; sequences.seq_pMLRT.name = "pMLRT"; sequences.seq_pMLRT.seqType = "both"; sequences.seq_pMLRT.comp = true; sequences.seq_pMLRT.data = `LOCUS pMLRT 5742 bp ds-DNA circular 08-JUL-2020 DEFINITION . KEYWORDS "accession:addgene_78234_146112" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    CDS             complement(2282..3073)
                    /label="Kan/neoR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    terminator      5566..5652
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    CDS             3405..3419
                    /label="enterokinase site"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    terminator      5486..5513
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    CDS             complement(2282..3076)
                    /label="NeoR/KanR"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer_bind     complement(2393..2412)
                    /label="Neo-F"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    terminator      2157..2251
                    /label="lambda t0 terminator"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295
    source          1..5742
                    /label="source"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    RBS             3339..3350
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    5538..5652
                    /label="on g-block 3"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    rep_origin      1443..1665
                    /label="pSC101 ori"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    CDS             3363..3398
                    /label="6xHN"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    3357..5357
                    /label="MLRT ORF"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    3339..3446
                    /label="on g-block 2"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer_bind     3003..3022
                    /label="Neo-R"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    Scar            5471..5478
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      5399..5470
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    CDS             complement(445..1395)
                    /label="Rep101"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    Scar            5383..5390
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer_bind     3255..3273
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    primer          3339..3361
                    /label="pMLRT FWD"
                    /note="sequence: CGTGAGCGTCTCTAAGGaaagaggagaaaggtaccatggg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer          5653..5672
                    /label="pMLRT FWD"
                    /note="sequence: TACTAGTAGCGGCCGCTGCAGccgccgccctagacctaggg"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer          complement(5631..5652)
                    /label="pMLRT REV"
                    /note="sequence: CTCACGCGTCTCAGCTACTGCAGCGGCCGCTACTAGTAatttgtcctactcaggagagcg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(5624..5652)
                    /label="pMLRT REV"
                    /note="sequence: atttgtcctactcaggagagcgttcaccg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(5631..5652)
                    /label="pMLRT REV"
                    /note="sequence: CTCACGCGTCTCAGCTAatttgtcctactcaggagagcg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          complement(4787..4806)
                    /label="pMLRT SDM REV"
                    /note="sequence: gggccagaatgacaagtggc"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          4807..4827
                    /label="pMLRT SDM FWD"
                    /note="sequence: cccatgcagtagaggcacttg"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 ttcaggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
      61 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
     121 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
     181 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
     241 tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa
     301 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
     361 tttgatatct aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     421 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     481 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg
     541 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     601 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
     661 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     721 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     781 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     841 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     901 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     961 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
    1021 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
    1081 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
    1141 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
    1201 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
    1261 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
    1321 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
    1381 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
    1441 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
    1501 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
    1561 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
    1621 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
    1681 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
    1741 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
    1801 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
    1861 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
    1921 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
    1981 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
    2041 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
    2101 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
    2161 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
    2221 gaggtcatta ctggatctat caacaggagt ccaagcgagc tctcgaaccc cagagtcccg
    2281 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
    2341 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
    2401 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
    2461 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
    2521 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
    2581 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
    2641 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
    2701 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
    2761 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
    2821 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
    2881 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
    2941 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
    3001 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
    3061 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
    3121 tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
    3181 ccttaccaga gggcgcccca gctggcaatt ccgacgtcta agaaaccatt attatcatga
    3241 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcacctc gagttgacag
    3301 ctagctcagt cctaggtact gtgctagcgg aattcattaa agaggagaaa ggtaccatgg
    3361 gtcataatca taatcataat cataatcata atcacaacgg tggagatgac gatgacaagg
    3421 gtggtcgaca agcttggatc cctgcaggcc tcagggcccg atcgatggga ccaatggggc
    3481 agcccctgca agtgttgacc ctaaatatag aagatgagta tcggctacat gagacctcaa
    3541 aagagccaga tgtttctcta gggtccacat ggctgtctga ttttcctcag gcctgggcgg
    3601 aaaccggggg catgggactg gcagttcgcc aagctcctct gatcatacct ctgaaagcaa
    3661 cctctacccc cgtgtccata aaacaatacc ccatgtcaca agaagccaga ctggggatca
    3721 agccccacat acagagactg ttggaccagg gaatactggt accctgccag tccccctgga
    3781 acacgcccct gctacccgtt aagaaaccag ggactaatga ttataggcct gtccaggatc
    3841 tgagagaagt caacaagcgg gtggaagaca tccaccccac cgtgcccaac ccttacaacc
    3901 tcttgagcgg gctcccaccg tcccaccagt ggtacactgt gcttgattta aaggatgcct
    3961 ttttctgcct gagactccac cccaccagtc agcctctctt cgcctttgag tggagagatc
    4021 cagagatggg aatctcagga caattgacct ggaccagact cccacagggt ttcaaaaaca
    4081 gtcccaccct gtttgatgag gcactgcaca gagacctagc agacttccgg atccagcacc
    4141 cagacttgat cctgctacag tacgtggatg acttactgct ggccgccact tctgagctag
    4201 actgccaaca aggtactcgg gccctgttac aaaccctagg gaacctcggg tatcgggcct
    4261 cggccaagaa agcccaaatt tgccagaaac aggtcaagta tctggggtat cttctaaaag
    4321 agggtcagag atggctgact gaggccagaa aagagactgt gatggggcag cctactccga
    4381 agacccctcg acaactaagg gagttcctag ggacggcagg cttctgtcgc ctctggatcc
    4441 ctgggtttgc agaaatggca gcccccttgt accctctcac caaaacgggg actctgttta
    4501 attggggccc agaccaacaa aaggcctatc aagaaatcaa gcaagctctt ctaactgccc
    4561 cagccctggg gttgccagat ttgactaagc cctttgaact ctttgtcgac gagaagcagg
    4621 gctacgccaa aggtgtccta acgcaaaaac tgggaccttg gcgtcggccg gtggcctacc
    4681 tgtccaaaaa gctagaccca gtagcagctg ggtggccccc ttgcctacgg atggtagcag
    4741 ccattgccgt actgacaaag gatgcaggca agctaaccat gggacagcca ctagtcattc
    4801 tggcccccca tgcagtagag gcactagtca aacaaccccc cgaccgctgg ctttccaacg
    4861 cccggatgac tcactatcag gccttgcttt tggacacgga ccgggtccag ttcggaccgg
    4921 tggtagccct gaacccggct acgctgctcc cactgcctga ggaagggctg caacacaact
    4981 gccttgatat cctggccgaa gcccacggaa cccgacccga cctaacggac cagccgctcc
    5041 cagacgccga ccacacctgg tacacggatg gaagcagtct cttacaagag ggacagcgta
    5101 aggcgggagc tgcggtgacc accgagaccg aggtaatctg ggctaaagcc ctgccagccg
    5161 ggacatccgc tcagcgggct gaactgatag cactcaccca ggccctaaag atggcagaag
    5221 gtaagaagct aaatgtttat actgatagcc gttatgcttt tgctactgcc catatccatg
    5281 gagaaatata cagaaggcgt gggttgctca catcagaagg caaagagatc aaaaataaag
    5341 acgagatctt taaatgacgc tgatagtgct agtgtagatc gctactagag ccaggcatca
    5401 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
    5461 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
    5521 ataataaatg tccagacctg caggcatgca agcctctaga ggcatcaaat aaaacgaaag
    5581 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
    5641 agtaggacaa atccgccgcc ctagacctag ggtacgggtt ttgctgcccg caaacgggct
    5701 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gc

//`

sequences.seq_pSB1A3GB3_backbone = {}; sequences.seq_pSB1A3GB3_backbone.varName = "seq_pSB1A3GB3_backbone"; sequences.seq_pSB1A3GB3_backbone.name = "pSB1A3-GB3 backbone"; sequences.seq_pSB1A3GB3_backbone.seqType = "both"; sequences.seq_pSB1A3GB3_backbone.comp = true; sequences.seq_pSB1A3GB3_backbone.data = `LOCUS pSB1A3-GB3_backbone 5268 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    primer_bind     4048..4066
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    promoter        632..662
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    terminator      2004..2061
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer_bind     3638..3657
                    /label="Amp-R"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    RBS             704..715
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    CDS             complement(3015..3875)
                    /label="AmpR"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    terminator      1444..1515
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer_bind     complement(2336..2355)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    source          1..5268
                    /label="source"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    protein_bind    4452..4485
                    /label="loxP"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    rep_origin      complement(2256..2844)
                    /label="ori"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer_bind     complement(2085..2102)
                    /label="L4440"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    promoter        complement(3876..3980)
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    protein_bind    670..686
                    /label="lac operator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    2002..4115
                    /label="pSB1A3 Region"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    CDS             722..1399
                    /label="mRFP1"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    protein_bind    596..617
                    /label="CAP binding site"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    misc_feature    3156..3156
                    /label="Point Mutation"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      1531..1558
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      4070..4113
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer          complement(4088..4115)
                    /label="BBa_Prefix & Promoter SDM Rev"
                    /note="sequence: cagaaatcatccttagcgaaagctaagg"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer          4116..4135
                    /label="BBa_Prefix & Promoter SDM Fwd"
                    /note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          2002..2019
                    /label="pSB1A3 fwd SapI"
                    /note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          complement(4093..4115)
                    /label="BsaI pSB1A3 Rev"
                    /note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          complement(4093..4115)
                    /label="pSB1A3 SapI rev"
                    /note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer          2002..2019
                    /label="BsaI pSB1A3 Fwd"
                    /note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          complement(4093..4115)
                    /label="Linear pSB1A3 BsmBI Rev"
                    /note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          2002..2023
                    /label="Linear pSB1A3 BsmBI Fwd"
                    /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295
    primer          2002..2019
                    /label="Linear pSB1A3 BsmBI Fwd"
                    /note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2

ORIGIN 

       1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
      61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
     121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
     181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
     241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
     301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
     361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
     421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
     481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
     721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
     781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
     841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
     901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
     961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
    1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
    1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
    1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
    1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
    1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
    1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
    1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
    1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
    1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
    1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
    1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
    1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
    1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
    1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
    1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
    1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
    1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
    2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
    2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
    2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
    2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
    2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
    2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
    3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
    3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
    4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
    4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
    4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
    4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
    4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
    4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
    4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
    4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
    4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
    4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
    4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
    4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
    4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
    4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
    4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
    4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
    5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
    5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
    5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
    5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa

//`

sequences.seq_pSB1C3_Forward_Primer = {}; sequences.seq_pSB1C3_Forward_Primer.varName = "seq_pSB1C3_Forward_Primer"; sequences.seq_pSB1C3_Forward_Primer.name = "pSB1C3 Forward Primer"; sequences.seq_pSB1C3_Forward_Primer.seqType = "linear"; sequences.seq_pSB1C3_Forward_Primer.comp = false; sequences.seq_pSB1C3_Forward_Primer.data = `LOCUS pSB1C3_Forward_Primer 41 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TATGATCGTC TCAcaaagtc agaacagatg tttgccgatg c

//`

sequences.seq_pSB1C3_Reverse_Primer = {}; sequences.seq_pSB1C3_Reverse_Primer.varName = "seq_pSB1C3_Reverse_Primer"; sequences.seq_pSB1C3_Reverse_Primer.name = "pSB1C3 Reverse Primer"; sequences.seq_pSB1C3_Reverse_Primer.seqType = "linear"; sequences.seq_pSB1C3_Reverse_Primer.comp = false; sequences.seq_pSB1C3_Reverse_Primer.data = `LOCUS pSB1C3_Reverse_Primer 45 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ACTGATCGTC TCTgttgtga agaaaaagtg aatgatgtag ccgtc

//`

sequences.seq_pSB1K3_containing_mRFP1 = {}; sequences.seq_pSB1K3_containing_mRFP1.varName = "seq_pSB1K3_containing_mRFP1"; sequences.seq_pSB1K3_containing_mRFP1.name = "pSB1K3 containing mRFP1"; sequences.seq_pSB1K3_containing_mRFP1.seqType = "both"; sequences.seq_pSB1K3_containing_mRFP1.comp = true; sequences.seq_pSB1K3_containing_mRFP1.data = `LOCUS pSB1K3_containing_mRFP1 3273 bp ds-DNA circular 07-JUL-2020 DEFINITION . KEYWORDS "accession:addgene_118082_252166" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    protein_bind    53..69
                    /label="lac operator"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    rep_origin      complement(1221..1809)
                    /label="ori"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer_bind     complement(1050..1067)
                    /label="L4440"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    RBS             87..98
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer_bind     2849..2868
                    /label="pENTR-R"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      827..898
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    primer_bind     2765..2784
                    /label="Kan-R"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    terminator      3084..3127
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    primer_bind     complement(1301..1320)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    source          1..3273
                    /label="source"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    CDS             complement(2042..2857)
                    /label="KanR"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    primer_bind     3062..3080
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    misc_feature    3130..3151
                    /label="BioBrick prefix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    promoter        15..45
                    /label="lac promoter"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    CDS             105..782
                    /label="mRFP1"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    protein_bind    3252..3273
                    /label="CAP binding site"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    948..968
                    /label="BioBrick suffix"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    terminator      969..1026
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    terminator      914..941
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2

ORIGIN 

       1 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      61 ggataacaat ttcacacata ctagagaaag aggagaaata ctagatggct tcctccgaag
     121 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
     181 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
     241 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
     301 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
     361 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
     421 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
     481 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
     541 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
     601 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
     661 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
     721 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
     781 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa
     841 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     901 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagtagcggc
     961 cgctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg
    1021 aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt
    1081 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    1141 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    1201 aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat
    1261 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    1321 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    1381 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    1441 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    1501 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    1561 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    1621 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    1681 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    1741 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    1801 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    1861 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    1921 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
    1981 tcgagtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta accaattctg
    2041 attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
    2101 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
    2161 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
    2221 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
    2281 ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt tcaacaggcc
    2341 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
    2401 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
    2461 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
    2521 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
    2581 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
    2641 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
    2701 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
    2761 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
    2821 gcctggagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
    2881 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
    2941 agagattttg agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg
    3001 atcagctcga gtgccacttg acgtctaaga aaccattatt atcatgacat taacctataa
    3061 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
    3121 tgatttctgg aattcgcggc cgcttctaga gcaatacgca aaccgcctct ccccgcgcgt
    3181 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
    3241 cgcaacgcaa ttaatgtgag ttagctcact cat

//`

sequences.seq_pSB1T3_Backbone = {}; sequences.seq_pSB1T3_Backbone.varName = "seq_pSB1T3_Backbone"; sequences.seq_pSB1T3_Backbone.name = "pSB1T3 Backbone"; sequences.seq_pSB1T3_Backbone.seqType = "both"; sequences.seq_pSB1T3_Backbone.comp = true; sequences.seq_pSB1T3_Backbone.data = `LOCUS pSB1T3_Backbone 2498 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    primer_bind     2389..2407
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer_bind     complement(120..137)
                    /label="L4440"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    18..38
                    /label="BioBrick suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    2456..2477
                    /label="BioBrick prefix"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    terminator      39..96
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    terminator      2411..2454
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    primer_bind     complement(371..390)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    rep_origin      complement(291..879)
                    /label="ori"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    CDS             1145..2329
                    /label="TetR"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86

ORIGIN 

       1 GGACTAGGTC TCATGCCtac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg
      61 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
     121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
     301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    1021 agtatatatg agtaaacttg gtctgacagc tcgagattct catgtttgac agcttatcat
    1081 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
    1141 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
    1201 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
    1261 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
    1321 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
    1381 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
    1441 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
    1501 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
    1561 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
    1621 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
    1681 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
    1741 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
    1801 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
    1861 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
    1921 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
    1981 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
    2041 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
    2101 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
    2161 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
    2221 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
    2281 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aactcgagtg
    2341 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
    2401 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctgaatt
    2461 cgcggccgct tctagaggCG TTAGAGACCT AGAGCATT

//`

sequences.seq_pUCSTS_FWD_BspMI = {}; sequences.seq_pUCSTS_FWD_BspMI.varName = "seq_pUCSTS_FWD_BspMI"; sequences.seq_pUCSTS_FWD_BspMI.name = "pUC-STS FWD BspMI"; sequences.seq_pUCSTS_FWD_BspMI.seqType = "linear"; sequences.seq_pUCSTS_FWD_BspMI.comp = false; sequences.seq_pUCSTS_FWD_BspMI.data = `LOCUS pUC-STS_FWD_BspMI 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ACAGTGGGTC TCCTAGTatg gtgtctgtga gtgag

//`

sequences.seq_pUCSTS_REV_BspMI = {}; sequences.seq_pUCSTS_REV_BspMI.varName = "seq_pUCSTS_REV_BspMI"; sequences.seq_pUCSTS_REV_BspMI.name = "pUC-STS REV BspMI"; sequences.seq_pUCSTS_REV_BspMI.seqType = "linear"; sequences.seq_pUCSTS_REV_BspMI.comp = false; sequences.seq_pUCSTS_REV_BspMI.data = `LOCUS pUC-STS_REV_BspMI 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ACAGTCGGTC TCCacaatta tatggccatg ctgcg

//`

sequences.seq_pUCSTS = {}; sequences.seq_pUCSTS.varName = "seq_pUCSTS"; sequences.seq_pUCSTS.name = "pUC-STS"; sequences.seq_pUCSTS.seqType = "both"; sequences.seq_pUCSTS.comp = true; sequences.seq_pUCSTS.data = `LOCUS pUC-STS 3622 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_35949_44214" SOURCE synthetic DNA construct 

 ORGANISM  synthetic DNA construct

FEATURES Location/Qualifiers 

    CDS             1632..2492
                    /label="AmpR"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    1154..1156
                    /label="Threonine inside BsaI site"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    primer_bind     complement(1262..1281)
                    /label="pRS-marker"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    primer_bind     3152..3171
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    source          23..1192
                    /label="STS"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    rep_origin      2663..3251
                    /label="ori"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    promoter        1527..1631
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    promoter        3575..3605
                    /label="lac promoter"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    primer_bind     complement(1850..1869)
                    /label="Amp-R"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    primer_bind     complement(1441..1459)
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    protein_bind    3539..3560
                    /label="CAP binding site"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc_feature    794..796
                    /label="Leu Codon inside BsaI Site "
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    primer_bind     1381..1403
                    /label="pGEX 3'"
                    /ApEinfo_revcolor=#d59687
                    /ApEinfo_fwdcolor=#d59687
    primer_bind     3405..3422
                    /label="L4440"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          complement(1173..1192)
                    /label="STS Reverse BspMI"
                    /note="sequence: ACTCCTACCTGCTTCaACAAttatatggccatgctgcgga"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer          complement(1193..1210)
                    /label="stsreverseprimer"
                    /note="sequence: aaataccgcagcggccgc"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    primer          23..44
                    /label="STS Forward BspMI"
                    /note="sequence: ACTGCTACCTGCTCCcTAGTatggtgtctgtgagtgagatcc"
                    /ApEinfo_revcolor=#d6b295
                    /ApEinfo_fwdcolor=#d6b295
    primer          1..22
                    /label="forward primer"
                    /note="sequence: ctagaagaaggaggattacaaa"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 ctagaagaag gaggattaca aaatggtgtc tgtgagtgag atccgcaaag ttcaaagggc
      61 agaaggccct gcaactgtat tggcgatagg cacagcaaat ccaccaaatt gtattgatca
     121 gagcacatat gctgattatt attttagagt aactaacagt gaacacatga ctgatctcaa
     181 gaagaagttt cagcgcattt gtgagagaac acaaatcaag aacagacata tgtacttaac
     241 agaagagata ctgaaagaga atcctaacat gtgcgcatac aaagcaccgt cgttggatgc
     301 aagggaagac atgatgatca gggaggtacc aagggttgga aaagaggctg caaccaaggc
     361 catcaaggaa tggggtcagc caatgtctaa gatcacacat ttgatcttct gcaccaccag
     421 cggtgttgca ttgcctggcg ttgattacga actcatcgta ctcttaggac tcgacccatc
     481 cgtcaagagg tacatgatgt accaccaagg ctgcttcgcc ggtggcactg tccttcgttt
     541 ggctaaggac ttggctgaaa acaacaagga tgctcgtgtg cttatcgttt gttctgagaa
     601 taccgcagtc actttccgtg gtcctagtga gacagacatg gatagtcttg tagggcaagc
     661 cttgtttgct gatggagctg ctgcgattat cattggttct gatcctgtgc cagaggttga
     721 aaagcctatc tttgaaattg tttcgactga tcaaaaactt gtccctaaca gccatggagc
     781 catcggtggt ctccttcgtg aagttgggct tacattctat cttaataaga gtgttcctga
     841 tattatttca caaaacatca atgatgcgct cagtaaagct tttgatccat tgggtatatc
     901 tgattataac tcaatatttt ggattgcaca ccctggtgga cgtgcaattt tggatcaggt
     961 tgaacagaaa gtgaacttga aaccagagaa gatgaatgcc actagagacg tgcttagcaa
    1021 ttacggtaac atgtcaagtg cgtgtgtgtt cttcatcatg gatttaatga ggaagaagtc
    1081 ccttgaagaa ggacttaaaa ctaccggtga aggacttgat tggggcgtac tttttggctt
    1141 tggtcctggt ctcactattg aaactgttgt tctccgcagc atggccatat aagcggccgc
    1201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    1261 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    1321 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    1381 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
    1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    1501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    1561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    1681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    1741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    1801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    1861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    1921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    1981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    2041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    2101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    2161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    2521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    2581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
    2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    3301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    3361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    3421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    3481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    3541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    3601 tgttgtgtgg aattgtgagc gt

//`

sequences.seq_Resveratrol_2x_Assembly = {}; sequences.seq_Resveratrol_2x_Assembly.varName = "seq_Resveratrol_2x_Assembly"; sequences.seq_Resveratrol_2x_Assembly.name = "Resveratrol 2x Assembly"; sequences.seq_Resveratrol_2x_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Assembly.comp = true; sequences.seq_Resveratrol_2x_Assembly.data = `LOCUS Resveratrol_2x_Assembly 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2436..2444
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2425..2433
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2414..2422
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    HIV-RT Binding Site join(2445..2448,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2403..2411
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAA

//`

sequences.seq_Resveratrol_2x_Comp_Assembly = {}; sequences.seq_Resveratrol_2x_Comp_Assembly.varName = "seq_Resveratrol_2x_Comp_Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.name = "Resveratrol 2x Comp Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Comp_Assembly.comp = true; sequences.seq_Resveratrol_2x_Comp_Assembly.data = `LOCUS Resveratrol_2x_Comp_Ass 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    HIV-RT Binding Site join(2445..2448,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2436..2444)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2425..2433)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    complement(2414..2422)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    complement(2403..2411)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2392..2400)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
    2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAA

//`

sequences.seq_Resveratrol_2x_Full_Part = {}; sequences.seq_Resveratrol_2x_Full_Part.varName = "seq_Resveratrol_2x_Full_Part"; sequences.seq_Resveratrol_2x_Full_Part.name = "Resveratrol 2x Full Part"; sequences.seq_Resveratrol_2x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_2x_Full_Part.comp = true; sequences.seq_Resveratrol_2x_Full_Part.data = `LOCUS Resveratrol_2x_Full_Par 2747 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    2414..2422
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Promoter            2632..2666
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site 2445..2554
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    HIV-RT Binding Site join(2744..2747,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2735..2743)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    misc_binding        2668..2690
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2713..2721)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Scar                2624..2631
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Terminator          2555..2623
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2425..2433
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2436..2444
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    2403..2411
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site    complement(2724..2732)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2702..2710)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2691..2699)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    gBlock              2501..2625
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
    2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
    2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
    2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
    2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
    2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAA

//`

sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.name = "Resveratrol 2x r_oligo Complement Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.comp = true; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_C 111 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    complement(42..50)
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    complement(53..61)
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    complement(64..72)
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    complement(75..83)
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site complement(86..94)
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
      61 CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAACG AGACCAACCT G

//`

sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.name = "Resveratrol 2x r_oligo Duplex 11-33 FWD 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.comp = false; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 23 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCCTAGCTGG AATTGTGAGC GGA

//`

sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.name = "Resveratrol 2x r_oligo Duplex 75-94 REV 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.comp = false; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TTTCCACACA TCGCCCACGC

//`

sequences.seq_Resveratrol_2x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.name = "Resveratrol 2x r_oligo Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.comp = true; sequences.seq_Resveratrol_2x_r_oligo_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_D 111 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site 42..50
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    misc_feature    53..61
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    64..72
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    75..83
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    86..94
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          complement(75..94)
                    /label="Resveratrol 2x r_oligo Duplex 75-94 REV 1"
                    /note="sequence: TTTCCACACATCGCCCACGC"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          11..33
                    /label="Resveratrol 2x r_oligo Duplex 11-33 FWD 1"
                    /note="sequence: TCCTAGCTGGAATTGTGAGCGGA"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
      61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAACG AGACCAACCT G

//`

sequences.seq_Resveratrol_4x_Assembly = {}; sequences.seq_Resveratrol_4x_Assembly.varName = "seq_Resveratrol_4x_Assembly"; sequences.seq_Resveratrol_4x_Assembly.name = "Resveratrol 4x Assembly"; sequences.seq_Resveratrol_4x_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Assembly.comp = true; sequences.seq_Resveratrol_4x_Assembly.data = `LOCUS Resveratrol_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    2447..2455
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2469..2477
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2458..2466
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Zinc Finger Site    2403..2411
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    2425..2433
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Zinc Finger Site    2436..2444
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2414..2422
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    HIV-RT Binding Site join(2489..2492,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2480..2488
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
    2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AA

//`

sequences.seq_Resveratrol_4x_Comp_Assembly = {}; sequences.seq_Resveratrol_4x_Comp_Assembly.varName = "seq_Resveratrol_4x_Comp_Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.name = "Resveratrol 4x Comp Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Comp_Assembly.comp = true; sequences.seq_Resveratrol_4x_Comp_Assembly.data = `LOCUS Resveratrol_4x_Comp_Ass 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Zinc Finger Site    complement(2469..2477)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2392..2400)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    complement(2458..2466)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    complement(2480..2488)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2414..2422)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    complement(2436..2444)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    HIV-RT Binding Site join(2489..2492,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2425..2433)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site    complement(2403..2411)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    complement(2447..2455)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
    2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT
    2461 CCACACATCG CCCACGCATG CCGTCCTCAA AA

//`

sequences.seq_Resveratrol_4x_Full_Part = {}; sequences.seq_Resveratrol_4x_Full_Part.varName = "seq_Resveratrol_4x_Full_Part"; sequences.seq_Resveratrol_4x_Full_Part.name = "Resveratrol 4x Full Part"; sequences.seq_Resveratrol_4x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_4x_Full_Part.comp = true; sequences.seq_Resveratrol_4x_Full_Part.data = `LOCUS Resveratrol_4x_Full_Par 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    Zinc Finger Site    complement(2823..2831)
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site    complement(2768..2776)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2392..2400
                        /label="ZFc Site"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    Zinc Finger Site    2403..2411
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2480..2488
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    terminator          2265..2308
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    Scar                2668..2675
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    primer_bind         complement(280..297)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Zinc Finger Site    complement(2812..2820)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         2243..2261
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    2414..2422
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    misc_feature        1351..1351
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    Promoter            2333..2367
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2779..2787)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    2436..2444
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    primer_bind         complement(531..550)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    Biobricking         2311..2332
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    HIV-RT Binding Site 2489..2598
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    2458..2466
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    HIV-RT Binding Site join(2832..2835,1..106)
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Zinc Finger Site    complement(2735..2743)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    misc_binding        2712..2734
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Terminator          2599..2667
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2425..2433
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site    2469..2477
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Backbone            197..2310
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Promoter            2676..2710
                        /label="J23100 Anderson Promoter"
                        /ApEinfo_revcolor=#d59687
                        /ApEinfo_fwdcolor=#d59687
    Zinc Finger Site    complement(2790..2798)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    gBlock              2545..2669
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    promoter            complement(2071..2175)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    CDS                 complement(1210..2070)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2757..2765)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    Zinc Finger Site    2447..2455
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         1833..1852
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_binding        2369..2391
                        /label="LacO"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    gBlock              53..196
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Zinc Finger Site    complement(2801..2809)
                        /label="PBSII Site"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    rep_origin          complement(451..1039)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    Zinc Finger Site    complement(2746..2754)
                        /label="Zif268 Site"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Biobricking         176..196
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    Terminator          107..175
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1

ORIGIN 

       1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
      61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
     121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
     181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
    1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
    1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
    2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
    2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
    2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
    2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
    2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
    2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
    2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
    2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
    2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
    2821 ATGCCGTCCT CAAAA

//`

sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.name = "Resveratrol 4x r_oligo Complement Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.comp = true; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_C 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    complement(42..50)
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    complement(53..61)
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    complement(64..72)
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    complement(75..83)
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    complement(86..94)
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    complement(97..105)
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    complement(108..116)
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    complement(119..127)
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    Zinc Finger Site complement(130..138)
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
      61 CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT CCACACATCG
     121 CCCACGCATG CCGTCCTCAA AACGAGACCA ACCTG

//`

sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.name = "Resveratrol 4x r_oligo Duplex 109-128 REV 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.comp = false; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 TCGCCCACGC ATTTTCCACA

//`

sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.name = "Resveratrol 4x r_oligo Duplex 39-58 FWD 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.comp = false; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 ATTGAGGACG GCATGCGTGG

//`

sequences.seq_Resveratrol_4x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.name = "Resveratrol 4x r_oligo Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.comp = true; sequences.seq_Resveratrol_4x_r_oligo_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_D 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    19..41
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Zinc Finger Site 42..50
                    /label="ZFc Site"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    misc_feature    53..61
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    64..72
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    75..83
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    86..94
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    97..105
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    108..116
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    119..127
                    /label="Zif268"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    130..138
                    /label="PBSII Site"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          39..58
                    /label="Resveratrol 4x r_oligo Duplex 39-58 FWD 1"
                    /note="sequence: ATTGAGGACGGCATGCGTGG"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    primer          complement(109..128)
                    /label="Resveratrol 4x r_oligo Duplex 109-128 REV 1"
                    /note="sequence: TCGCCCACGCATTTTCCACA"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3

ORIGIN 

       1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
      61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG TGGAAAATGC
     121 GTGGGCGATG TGTGGAAAAA AACGAGACCA ACCTG

//`

sequences.seq_r_oligo_Flipper = {}; sequences.seq_r_oligo_Flipper.varName = "seq_r_oligo_Flipper"; sequences.seq_r_oligo_Flipper.name = "r_oligo Flipper"; sequences.seq_r_oligo_Flipper.seqType = "both"; sequences.seq_r_oligo_Flipper.comp = true; sequences.seq_r_oligo_Flipper.data = `LOCUS r_oligo_Flipper 3220 bp ds-DNA circular 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    Biobricking         2111..2132
                        /label="BioBrick Prefix "
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    primer_bind         2043..2061
                        /label="pBRforEco"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    BioBrick            2964..3009
                        /label="BBa_B0011"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    BioBrick            2915..2955
                        /label="BBa_B0012"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    misc_feature        1151..1151
                        /label="Point Mutation"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    BioBrick            2915..3009
                        /label="BBa_B0014 (double terminator)"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    gBlock              2111..3072
                        /label="gBlock A"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    Scar                2956..2963
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    CDS                 complement(1010..1870)
                        /label="AmpR"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    BioBrick            2203..2908
                        /label="BBa_E1010 (RFP)"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    stem_loop           2976..2998
                        /label="stem loop"
                        /ApEinfo_revcolor=#ff9ccd
                        /ApEinfo_fwdcolor=#ff9ccd
    primer_bind         1633..1652
                        /label="Amp-R"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    primer_bind         complement(331..350)
                        /label="pBR322ori-F"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    HIV-RT Binding Site 3017..3126
                        /label="C* HTBS"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    terminator          2065..2108
                        /label="bacterial terminator"
                        /ApEinfo_revcolor=#ffef86
                        /ApEinfo_fwdcolor=#ffef86
    stop                2878..2883
                        /label="double stop codon"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    primer_bind         complement(80..97)
                        /label="L4440"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    Backbone            join(3217..3220,1..2110)
                        /label="Mutated pSB1A3"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e
    gBlock              3073..3216
                        /label="gBlock B"
                        /ApEinfo_revcolor=#85dae9
                        /ApEinfo_fwdcolor=#85dae9
    Scar                2175..2182
                        /label="BioBrick Scar"
                        /ApEinfo_revcolor=#9eafd2
                        /ApEinfo_fwdcolor=#9eafd2
    rep_origin          complement(251..839)
                        /label="ori"
                        /ApEinfo_revcolor=#b1ff67
                        /ApEinfo_fwdcolor=#b1ff67
    rbs                 2190..2194
                        /label="strong RBS"
                        /ApEinfo_revcolor=#c7b0e3
                        /ApEinfo_fwdcolor=#c7b0e3
    protein             2203..2908
                        /label="RFP"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    Terminator          3127..3195
                        /label="B0054"
                        /ApEinfo_revcolor=#c6c9d1
                        /ApEinfo_fwdcolor=#c6c9d1
    Biobricking         3196..3216
                        /label="BioBrick Suffix"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    stop                2887..2892
                        /label="double stop codon"
                        /ApEinfo_revcolor=#75c6a9
                        /ApEinfo_fwdcolor=#75c6a9
    promoter            complement(1871..1975)
                        /label="AmpR promoter"
                        /ApEinfo_revcolor=#faac61
                        /ApEinfo_fwdcolor=#faac61
    start               2203..2205
                        /label="start codon"
                        /ApEinfo_revcolor=#84b0dc
                        /ApEinfo_fwdcolor=#84b0dc
    BioBrick            2183..2197
                        /label="BBa_B0030 (strong RBS)"
                        /ApEinfo_revcolor=#b4abac
                        /ApEinfo_fwdcolor=#b4abac
    misc_feature        2183..3009
                        /label="From BBa_K3114015"
                        /ApEinfo_revcolor=#b7e6d7
                        /ApEinfo_fwdcolor=#b7e6d7
    misc_feature        2133..2167
                        /label="BBa_J23105"
                        /ApEinfo_revcolor=#f58a5e
                        /ApEinfo_fwdcolor=#f58a5e

ORIGIN 

       1 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
      61 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     241 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     661 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    1021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    1081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    1141 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    1201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    1261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    1321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    1381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    1441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    1501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    1561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    1621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    1681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatat
    1741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    1801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    1861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    1921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    1981 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    2041 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
    2101 atgatttctg gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacta
    2161 tgctagcCGA GACCtactag agattaaaga ggagaaatac taatggcttc ctccgaagac
    2221 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
    2281 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
    2341 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
    2401 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
    2461 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
    2521 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
    2581 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
    2641 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
    2701 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
    2761 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
    2821 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
    2881 taacgctgat agtgctagtg tagatcgcta ataatcacac tggctcacct tcgggtgggc
    2941 ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt
    3001 ttattatttG GTCTCGAAAA AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT
    3061 AGAGCATCAG ACTTTTAATC TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT
    3121 TTTTTTatta gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt
    3181 ggggttatca ttgggtacta gtagcggccg ctgcagagtc

//`

sequences.seq_SapI_FWD_Primer = {}; sequences.seq_SapI_FWD_Primer.varName = "seq_SapI_FWD_Primer"; sequences.seq_SapI_FWD_Primer.name = "SapI FWD Primer"; sequences.seq_SapI_FWD_Primer.seqType = "linear"; sequences.seq_SapI_FWD_Primer.comp = false; sequences.seq_SapI_FWD_Primer.data = `LOCUS SapI_FWD_Primer 32 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 AAAGCTCTTC AGGAagtccg gcaaaaaagg gc

//`

sequences.seq_SapI_Rev_Primer = {}; sequences.seq_SapI_Rev_Primer.varName = "seq_SapI_Rev_Primer"; sequences.seq_SapI_Rev_Primer.name = "SapI Rev Primer"; sequences.seq_SapI_Rev_Primer.seqType = "linear"; sequences.seq_SapI_Rev_Primer.comp = false; sequences.seq_SapI_Rev_Primer.data = `LOCUS SapI_Rev_Primer 37 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN 

       1 CCCGCTCTTC AGTGcagaaa tcatccttag cgaaagc

//`

sequences.seq_STS_intro_gblock = {}; sequences.seq_STS_intro_gblock.varName = "seq_STS_intro_gblock"; sequences.seq_STS_intro_gblock.name = "STS intro gblock"; sequences.seq_STS_intro_gblock.seqType = "linear"; sequences.seq_STS_intro_gblock.comp = true; sequences.seq_STS_intro_gblock.data = `LOCUS STS_intro_gblock 474 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    40..74
                    /label="BBa_J23111"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_feature    123..431
                    /label="PBSII"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    misc_feature    99..112
                    /label="BBa_J61101"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    Biobricking     18..39
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_binding    75..98
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    432..461
                    /label="Spacer"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          18..41
                    /label="forward Prefix-Promoter-PBS2-sapI primer"
                    /note="sequence: AAAGCTCTTCACACgaattcgcggccgcttctagagtt"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer          complement(439..461)
                    /label="reverse Prefix-Promoter-PBS2-sapI primer"
                    /note="sequence: AAAGCTCTTCAACTactacctccacttccacctccac"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3

ORIGIN 

       1 ACCATCGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
      61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGACAGGAC CCtaaataca
     121 tgATGCATCA CCATCACCAT CATCCAGGCG AGAAACCGTA CGCCTGCCCC GAATGCGGTA
     181 AATCATTTAG TCAGCGGGCG AACTTACGCG CACACCAGCG AACCCATACC GGAGAAAAAC
     241 CGTATAAATG TCCGGAATGT GGTAAAAGCT TTAGCCGTTC GGATCATCTG ACGACTCACC
     301 AACGCACACA CACCGGGGAA AAGCCGTATA AATGCCCTGA GTGTGGTAAG TCGTTCTCCC
     361 GTTCTGACGT TCTGGTGCGC CATCAGCGTA CGCATACTGG TGGCGGGAGC GGCGGCGGAA
     421 GTGGTGGCTC Cggtggaagt ggaggtggaa gtggaggtag tAGAGACCTC CTTT

//`

sequences.seq_STS_outro_gblock = {}; sequences.seq_STS_outro_gblock.varName = "seq_STS_outro_gblock"; sequences.seq_STS_outro_gblock.name = "STS outro gblock"; sequences.seq_STS_outro_gblock.seqType = "linear"; sequences.seq_STS_outro_gblock.comp = true; sequences.seq_STS_outro_gblock.data = `LOCUS STS_outro_gblock 129 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    stem_loop       27..78
                    /label="T0 terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    Biobricking     79..99
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 gtcaagctgc catgacgagG GTCTCAttgt tcagaacgct cggtcttgca caccgggcgt
      61 tttttctttg tgagtccata ctagtagcgg ccgctgcagT GCCAGAGACC tccaagagag
     121 tgaagggat

//`

sequences.seq_ultramer_duplex_2 = {}; sequences.seq_ultramer_duplex_2.varName = "seq_ultramer_duplex_2"; sequences.seq_ultramer_duplex_2.name = "ultramer duplex 2"; sequences.seq_ultramer_duplex_2.seqType = "linear"; sequences.seq_ultramer_duplex_2.comp = true; sequences.seq_ultramer_duplex_2.data = `LOCUS ultramer_duplex_2 215 bp ds-DNA linear 06-SEP-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_binding    54..76
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    RBS             87..98
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    CDS             111..146
                    /label="6xHN"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    18..52
                    /label="'BBa_J23109'"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    CDS             153..167
                    /label="enterokinase site"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 TTATCCCGTC TCATGCGttt acagctagct cagtcctagg gactgtgcta gcTGGAATTG
      61 TGAGCGGATA ACAATTcgtt gcaactaaag aggagaaagg taccatgggt cataatcata
     121 atcataatca taatcataat cacaacggtg gagatgacga tgacaagggt ggtcgacaag
     181 cttggatccc agcaggccTT GGTGAGACGG TGATT

//`

sequences.seq_Updated_Assembly_in_Amp = {}; sequences.seq_Updated_Assembly_in_Amp.varName = "seq_Updated_Assembly_in_Amp"; sequences.seq_Updated_Assembly_in_Amp.name = "Updated Assembly in Amp"; sequences.seq_Updated_Assembly_in_Amp.seqType = "both"; sequences.seq_Updated_Assembly_in_Amp.comp = true; sequences.seq_Updated_Assembly_in_Amp.data = `LOCUS Updated_Assembly_in_Amp 7888 bp ds-DNA circular 24-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    None            5477..5511
                    /label="'BBa_J23109'"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    Scar            5415..5422
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      2065..2108
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    1151..1151
                    /label="Point Mutation"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    Scar            7678..7685
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      7777..7863
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    misc_binding    5513..5535
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    primer_bind     1633..1652
                    /label="Amp-R"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    misc_feature    2283..3968
                    /label="HIVRT p66"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    primer_bind     2043..2061
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    CDS             5570..5605
                    /label="6xHN"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_binding    2169..2191
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    RBS             3977..3988
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    CDS             complement(1010..1870)
                    /label="AmpR"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    Biobricking     7864..7884
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Biobricking     2111..2132
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    RBS             5546..5557
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    RBS             2265..2276
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    terminator      7606..7677
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    misc_feature    3995..5320
                    /label="HIVRT P51"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    terminator      7693..7720
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    rep_origin      complement(251..839)
                    /label="ori"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    Scar            7590..7597
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    2133..2167
                    /label="BBa_J23109'"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    5564..7564
                    /label="MLRT ORF"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_feature    2207..2256
                    /label="sTRSV HHRz"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    primer_bind     complement(331..350)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    primer_bind     complement(80..97)
                    /label="L4440"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    terminator      5343..5414
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    terminator      5430..5457
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    promoter        complement(1871..1975)
                    /label="AmpR promoter"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    Scar            5327..5334
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    misc_feature    5612..5626
                    /label="Enterokinase Site"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
      61 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     241 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     661 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    1021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    1081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    1141 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    1201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    1261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    1321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    1381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    1441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    1501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    1561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    1621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    1681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatat
    1741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    1801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    1861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    1921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    1981 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    2041 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
    2101 atgatttctg GAATTCGCGG CCGCTTCTAG AGtttacagc tagctcagtc ctagggactg
    2161 tgctagcTGG AATTGTGAGC GGATAACAAT Tctatggact atgtttCTGT CACCGGATGT
    2221 GCTTTCCGGT CTGATGAGTC CGTGAGGACG AAACAGtacc agtgaaagag gagaaaGGAT
    2281 agatgccgat tagcccgatt gaaaccgttc cggttaaact gaaaccgggt atggatggtc
    2341 cgaaagttaa acagtggcct ctgaccgaag aaaaaatcaa agcactggtt gaaatctgca
    2401 ccgagatgga aaaagaaggc aaaattagca aaatcggtcc ggaaaatccg tataatacac
    2461 cggtttttgc cattaagaaa aaagatagca ccaaatggcg caaactggtg gattttcgtg
    2521 aactgaataa acgcacccag gatttttggg aagttcagct gggtattccg catccggcag
    2581 gtctgaaaca gaaaaaaagc gttaccgttc tggatgttgg tgatgcatat tttagcgttc
    2641 cgctggataa agatttccgt aaatataccg catttaccat cccgagcatt aataacgaaa
    2701 caccgggtat tcgctatcag tataatgttc tgccgcaggg ttggaaaggt agtccggcaa
    2761 tttttcagtg tagcatgacc aaaattctgg aaccgtttcg taaacagaat ccggatattg
    2821 tgatctacca gtatatggat gatctgtatg ttggtagcga tctggaaatt ggtcagcatc
    2881 gtaccaaaat tgaagaactg cgtcagcatc tgctgcgttg gggttttacc acaccggata
    2941 aaaaacatca gaaagaaccg ccttttctgt ggatgggtta tgaactgcat ccggataaat
    3001 ggaccgttca gccgattgtt ctgccggaaa aagatagctg gaccgttaat gatattcaga
    3061 aactggtggg taaactgaat tgggcaagcc agatttatgc cggtattaaa gttcgtcagc
    3121 tgtgtaaact gctgcgtggc accaaagcac tgaccgaagt tgttccgctg acagaagaag
    3181 cagaactgga actggcagaa aatcgtgaaa ttctgaaaga accggttcac ggcgtttatt
    3241 atgatccgag caaagatctg attgccgaaa ttcagaaaca gggtcagggt cagtggacct
    3301 atcagattta tcaagaaccg tttaaaaacc tgaaaaccgg caaatatgca cgtatgaaag
    3361 gtgcacatac caacgatgtt aaacagctga ccgaagcagt tcagaaaatt gcaaccgaaa
    3421 gcattgtgat ttggggtaaa accccgaaat tcaaactgcc gattcagaaa gaaacctggg
    3481 aagcatggtg gaccgaatat tggcaggcaa cctggattcc ggaatgggaa tttgttaata
    3541 cccctccgct ggttaaactg tggtatcagc tggaaaaaga accgattatt ggtgccgaaa
    3601 ccttttatgt tgatggtgca gccaatcgtg aaaccaaact gggtaaagca ggttatgtta
    3661 ccgatcgtgg tcgtcagaaa gtggtgccgc tgaccgatac caccaatcag aaaaccgaac
    3721 tacaggcaat tcatctggca ctacaggata gcggtctgga agttaatatt gttaccgata
    3781 gccagtatgc cctgggtatt attcaggcac agccggataa aagcgaaagc gaactggtta
    3841 gccagattat tgaacagctg atcaaaaaag aaaaagtgta cctggcatgg gttccggcac
    3901 ataaaggtat tggtggtaat gaacaggttg atggtctggt tagcgcaggt attcgtaaag
    3961 ttctgtaata ttagtgaaag aggagaaata ctagatgccg attagcccga ttgaaaccgt
    4021 tccggttaaa ctgaaaccgg gtatggatgg tccgaaagtt aaacagtggc ctctgaccga
    4081 agaaaaaatc aaagcactgg ttgaaatctg caccgagatg gaaaaagaag gcaaaattag
    4141 caaaatcggt ccggaaaatc cgtataatac accggttttt gccattaaga aaaaagatag
    4201 caccaaatgg cgcaaactgg tggattttcg tgaactgaat aaacgcaccc aggatttttg
    4261 ggaagttcag ctgggtattc cgcatccggc aggtctgaaa cagaaaaaaa gcgttaccgt
    4321 tctggatgtt ggtgatgcat attttagcgt tccgctggat aaagatttcc gtaaatatac
    4381 cgcatttacc atcccgagca ttaataacga aacaccgggt attcgctatc agtataatgt
    4441 tctgccgcag ggttggaaag gtagtccggc aatttttcag tgtagcatga ccaaaattct
    4501 ggaaccgttt cgtaaacaga atccggatat tgtgatctac cagtatatgg atgatctgta
    4561 tgttggtagc gatctggaaa ttggtcagca tcgtaccaaa attgaagaac tgcgtcagca
    4621 tctgctgcgt tggggtttta ccacaccgga taaaaaacat cagaaagaac cgccttttct
    4681 gtggatgggt tatgaactgc atccggataa atggaccgtt cagccgattg ttctgccgga
    4741 aaaagatagc tggaccgtta atgatattca gaaactggtg ggtaaactga attgggcaag
    4801 ccagatttat gccggtatta aagttcgtca gctgtgtaaa ctgctgcgtg gcaccaaagc
    4861 actgaccgaa gttgttccgc tgacagaaga agcagaactg gaactggcag aaaatcgtga
    4921 aattctgaaa gaaccggttc acggcgttta ttatgatccg agcaaagatc tgattgccga
    4981 aattcagaaa cagggtcagg gtcagtggac ctatcagatt tatcaagaac cgtttaaaaa
    5041 cctgaaaacc ggcaaatatg cacgtatgaa aggtgcacat accaacgatg ttaaacagct
    5101 gaccgaagca gttcagaaaa ttgcaaccga aagcattgtg atttggggta aaaccccgaa
    5161 attcaaactg ccgattcaga aagaaacctg ggaagcatgg tggaccgaat attggcaggc
    5221 aacctggatt ccggaatggg aatttgttaa tacccctccg ctggttaaac tgtggtatca
    5281 gctggaaaaa gaaccgatta ttggtgccga aaccttttaa gatcgctact agagccaggc
    5341 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
    5401 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
    5461 atatactaga agcggcTTTA CAGCTAGCTC AGTCCTAGGG ACTGTGCTAG CTGGAATTGT
    5521 GAGCGGATAA CAATTCGTTG CAACTAAAGA GGAGAAAGGT ACCATGGGGC ATAATCACAA
    5581 CCACAATCAC AATCATAACC ACAATGGAGG TGACGATGAC GATAAGGGTG GCCGCCAGGC
    5641 CTGGATCCCA GCCGGCCCCA Aggcccgatc gatgggacca atggggcagc ccctgcaagt
    5701 gttgacccta aatatagaag atgagtatcg gctacatgag acctcaaaag agccagatgt
    5761 ttctctaggg tccacatggc tgtctgattt tcctcaggcc tgggcggaaa ccgggggcat
    5821 gggactggca gttcgccaag ctcctctgat catacctctg aaagcaacct ctacccccgt
    5881 gtccataaaa caatacccca tgtcacaaga agccagactg gggatcaagc cccacataca
    5941 gagactgttg gaccagggaa tactggtacc ctgccagtcc ccctggaaca cgcccctgct
    6001 acccgttaag aaaccaggga ctaatgatta taggcctgtc caggatctga gagaagtcaa
    6061 caagcgggtg gaagacatcc accccaccgt gcccaaccct tacaacctct tgagcgggct
    6121 cccaccgtcc caccagtggt acactgtgct tgatttaaag gatgcctttt tctgcctgag
    6181 actccacccc accagtcagc ctctcttcgc ctttgagtgg agagatccag agatgggaat
    6241 ctcaggacaa ttgacctgga ccagactccc acagggtttc aaaaacagtc ccaccctgtt
    6301 tgatgaggca ctgcacagag acctagcaga cttccggatc cagcacccag acttgatcct
    6361 gctacagtac gtggatgact tactgctggc cgccacttct gagctagact gccaacaagg
    6421 tactcgggcc ctgttacaaa ccctagggaa cctcgggtat cgggcctcgg ccaagaaagc
    6481 ccaaatttgc cagaaacagg tcaagtatct ggggtatctt ctaaaagagg gtcagagatg
    6541 gctgactgag gccagaaaag agactgtgat ggggcagcct actccgaaga cccctcgaca
    6601 actaagggag ttcctaggga cggcaggctt ctgtcgcctc tggatccctg ggtttgcaga
    6661 aatggcagcc cccttgtacc ctctcaccaa aacggggact ctgtttaatt ggggcccaga
    6721 ccaacaaaag gcctatcaag aaatcaagca agctcttcta actgccccag ccctggggtt
    6781 gccagatttg actaagccct ttgaactctt tgtcgacgag aagcagggct acgccaaagg
    6841 tgtcctaacg caaaaactgg gaccttggcg tcggccggtg gcctacctgt ccaaaaagct
    6901 agacccagta gcagctgggt ggcccccttg cctacggatg gtagcagcca ttgccgtact
    6961 gacaaaggat gcaggcaagc taaccatggg acagccactt gtcattctgg ccccccatgc
    7021 agtagaggca cttgtcaaac aaccccccga ccgctggctt tccaacgccc ggatgactca
    7081 ctatcaggcc ttgcttttgg acacggaccg ggtccagttc ggaccggtgg tagccctgaa
    7141 cccggctacg ctgctcccac tgcctgagga agggctgcaa cacaactgcc ttgatatcct
    7201 ggccgaagcc cacggaaccc gacccgacct aacggaccag ccgctcccag acgccgacca
    7261 cacctggtac acggatggaa gcagtctctt acaagaggga cagcgtaagg cgggagctgc
    7321 ggtgaccacc gagaccgagg taatctgggc taaagccctg ccagccggga catccgctca
    7381 gcgggctgaa ctgatagcac tcacccaggc cctaaagatg gcagaaggta agaagctaaa
    7441 tgtttatact gatagccgtt atgcttttgc tactgcccat atccatggag aaatatacag
    7501 aaggcgtggg ttgctcacat cagaaggcaa agagatcaaa aataaagacg agatctttaa
    7561 atgacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
    7621 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
    7681 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatata ataaatgtcc
    7741 agaAGGGcct tcaggcatgc aagcctctgg aggcatcaaa taaaacgaaa ggctcagtcg
    7801 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
    7861 aatTACTAGT AGCGGCCGCT GCAGAGTC

//`

sequences.seq_Updated_Assembly_in_Kan = {}; sequences.seq_Updated_Assembly_in_Kan.varName = "seq_Updated_Assembly_in_Kan"; sequences.seq_Updated_Assembly_in_Kan.name = "Updated Assembly in Kan"; sequences.seq_Updated_Assembly_in_Kan.seqType = "both"; sequences.seq_Updated_Assembly_in_Kan.comp = true; sequences.seq_Updated_Assembly_in_Kan.data = `LOCUS Updated_Assembly_in_Kan 7935 bp ds-DNA circular 24-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    terminator      7746..7789
                    /label="bacterial terminator"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    1741..3066
                    /label="HIVRT P51"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    5626..5630
                    /label="BioBrick suffix"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    RBS             11..22
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    misc_feature    3358..3372
                    /label="Enterokinase Site"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    7792..7792
                    /label="BioBrick prefix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    Biobricking     7793..7813
                    /label="BioBrick Prefix "
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    CDS             complement(6704..7519)
                    /label="KanR"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    Scar            3073..3080
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer_bind     7427..7446
                    /label="Kan-R"
                    /ApEinfo_revcolor=#c6c9d1
                    /ApEinfo_fwdcolor=#c6c9d1
    misc_binding    7850..7872
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    misc_feature    29..1714
                    /label="HIVRT p66"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Scar            5336..5343
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      3176..3203
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    terminator      3089..3160
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    CDS             3316..3351
                    /label="6xHN"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    source          5626..7792
                    /label="source"
                    /ApEinfo_revcolor=#b4abac
                    /ApEinfo_fwdcolor=#b4abac
    terminator      5439..5466
                    /label="T7Te terminator"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    misc_feature    7814..7848
                    /label="BBa_J23109'"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    misc_feature    join(7888..7935,1..2)
                    /label="sTRSV HHRz"
                    /ApEinfo_revcolor=#f8d3a9
                    /ApEinfo_fwdcolor=#f8d3a9
    terminator      5523..5609
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#b7e6d7
                    /ApEinfo_fwdcolor=#b7e6d7
    primer_bind     7724..7742
                    /label="pBRforEco"
                    /ApEinfo_revcolor=#75c6a9
                    /ApEinfo_fwdcolor=#75c6a9
    RBS             1723..1734
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#c7b0e3
                    /ApEinfo_fwdcolor=#c7b0e3
    Biobricking     5610..5625
                    /label="BioBrick Suffix"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc
    None            3223..3257
                    /label="'BBa_J23109'"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    primer_bind     7511..7530
                    /label="pENTR-R"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    primer_bind     complement(5963..5982)
                    /label="pBR322ori-F"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    Scar            3161..3168
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    RBS             3292..3303
                    /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                    /ApEinfo_revcolor=#faac61
                    /ApEinfo_fwdcolor=#faac61
    rep_origin      complement(5883..6471)
                    /label="ori"
                    /ApEinfo_revcolor=#85dae9
                    /ApEinfo_fwdcolor=#85dae9
    primer_bind     complement(5712..5729)
                    /label="L4440"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd
    terminator      5631..5688
                    /label="his operon terminator"
                    /ApEinfo_revcolor=#b1ff67
                    /ApEinfo_fwdcolor=#b1ff67
    Scar            5424..5431
                    /label="BioBrick Scar"
                    /ApEinfo_revcolor=#9eafd2
                    /ApEinfo_fwdcolor=#9eafd2
    terminator      5352..5423
                    /label="rrnB T1 terminator"
                    /ApEinfo_revcolor=#f58a5e
                    /ApEinfo_fwdcolor=#f58a5e
    misc_feature    3310..5310
                    /label="MLRT ORF"
                    /ApEinfo_revcolor=#ffef86
                    /ApEinfo_fwdcolor=#ffef86
    misc_binding    3259..3281
                    /label="LacO"
                    /ApEinfo_revcolor=#84b0dc
                    /ApEinfo_fwdcolor=#84b0dc

ORIGIN 

       1 AGtaccagtg aaagaggaga aaGGATagat gccgattagc ccgattgaaa ccgttccggt
      61 taaactgaaa ccgggtatgg atggtccgaa agttaaacag tggcctctga ccgaagaaaa
     121 aatcaaagca ctggttgaaa tctgcaccga gatggaaaaa gaaggcaaaa ttagcaaaat
     181 cggtccggaa aatccgtata atacaccggt ttttgccatt aagaaaaaag atagcaccaa
     241 atggcgcaaa ctggtggatt ttcgtgaact gaataaacgc acccaggatt tttgggaagt
     301 tcagctgggt attccgcatc cggcaggtct gaaacagaaa aaaagcgtta ccgttctgga
     361 tgttggtgat gcatatttta gcgttccgct ggataaagat ttccgtaaat ataccgcatt
     421 taccatcccg agcattaata acgaaacacc gggtattcgc tatcagtata atgttctgcc
     481 gcagggttgg aaaggtagtc cggcaatttt tcagtgtagc atgaccaaaa ttctggaacc
     541 gtttcgtaaa cagaatccgg atattgtgat ctaccagtat atggatgatc tgtatgttgg
     601 tagcgatctg gaaattggtc agcatcgtac caaaattgaa gaactgcgtc agcatctgct
     661 gcgttggggt tttaccacac cggataaaaa acatcagaaa gaaccgcctt ttctgtggat
     721 gggttatgaa ctgcatccgg ataaatggac cgttcagccg attgttctgc cggaaaaaga
     781 tagctggacc gttaatgata ttcagaaact ggtgggtaaa ctgaattggg caagccagat
     841 ttatgccggt attaaagttc gtcagctgtg taaactgctg cgtggcacca aagcactgac
     901 cgaagttgtt ccgctgacag aagaagcaga actggaactg gcagaaaatc gtgaaattct
     961 gaaagaaccg gttcacggcg tttattatga tccgagcaaa gatctgattg ccgaaattca
    1021 gaaacagggt cagggtcagt ggacctatca gatttatcaa gaaccgttta aaaacctgaa
    1081 aaccggcaaa tatgcacgta tgaaaggtgc acataccaac gatgttaaac agctgaccga
    1141 agcagttcag aaaattgcaa ccgaaagcat tgtgatttgg ggtaaaaccc cgaaattcaa
    1201 actgccgatt cagaaagaaa cctgggaagc atggtggacc gaatattggc aggcaacctg
    1261 gattccggaa tgggaatttg ttaatacccc tccgctggtt aaactgtggt atcagctgga
    1321 aaaagaaccg attattggtg ccgaaacctt ttatgttgat ggtgcagcca atcgtgaaac
    1381 caaactgggt aaagcaggtt atgttaccga tcgtggtcgt cagaaagtgg tgccgctgac
    1441 cgataccacc aatcagaaaa ccgaactaca ggcaattcat ctggcactac aggatagcgg
    1501 tctggaagtt aatattgtta ccgatagcca gtatgccctg ggtattattc aggcacagcc
    1561 ggataaaagc gaaagcgaac tggttagcca gattattgaa cagctgatca aaaaagaaaa
    1621 agtgtacctg gcatgggttc cggcacataa aggtattggt ggtaatgaac aggttgatgg
    1681 tctggttagc gcaggtattc gtaaagttct gtaatattag tgaaagagga gaaatactag
    1741 atgccgatta gcccgattga aaccgttccg gttaaactga aaccgggtat ggatggtccg
    1801 aaagttaaac agtggcctct gaccgaagaa aaaatcaaag cactggttga aatctgcacc
    1861 gagatggaaa aagaaggcaa aattagcaaa atcggtccgg aaaatccgta taatacaccg
    1921 gtttttgcca ttaagaaaaa agatagcacc aaatggcgca aactggtgga ttttcgtgaa
    1981 ctgaataaac gcacccagga tttttgggaa gttcagctgg gtattccgca tccggcaggt
    2041 ctgaaacaga aaaaaagcgt taccgttctg gatgttggtg atgcatattt tagcgttccg
    2101 ctggataaag atttccgtaa atataccgca tttaccatcc cgagcattaa taacgaaaca
    2161 ccgggtattc gctatcagta taatgttctg ccgcagggtt ggaaaggtag tccggcaatt
    2221 tttcagtgta gcatgaccaa aattctggaa ccgtttcgta aacagaatcc ggatattgtg
    2281 atctaccagt atatggatga tctgtatgtt ggtagcgatc tggaaattgg tcagcatcgt
    2341 accaaaattg aagaactgcg tcagcatctg ctgcgttggg gttttaccac accggataaa
    2401 aaacatcaga aagaaccgcc ttttctgtgg atgggttatg aactgcatcc ggataaatgg
    2461 accgttcagc cgattgttct gccggaaaaa gatagctgga ccgttaatga tattcagaaa
    2521 ctggtgggta aactgaattg ggcaagccag atttatgccg gtattaaagt tcgtcagctg
    2581 tgtaaactgc tgcgtggcac caaagcactg accgaagttg ttccgctgac agaagaagca
    2641 gaactggaac tggcagaaaa tcgtgaaatt ctgaaagaac cggttcacgg cgtttattat
    2701 gatccgagca aagatctgat tgccgaaatt cagaaacagg gtcagggtca gtggacctat
    2761 cagatttatc aagaaccgtt taaaaacctg aaaaccggca aatatgcacg tatgaaaggt
    2821 gcacatacca acgatgttaa acagctgacc gaagcagttc agaaaattgc aaccgaaagc
    2881 attgtgattt ggggtaaaac cccgaaattc aaactgccga ttcagaaaga aacctgggaa
    2941 gcatggtgga ccgaatattg gcaggcaacc tggattccgg aatgggaatt tgttaatacc
    3001 cctccgctgg ttaaactgtg gtatcagctg gaaaaagaac cgattattgg tgccgaaacc
    3061 ttttaagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
    3121 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
    3181 accttcgggt gggcctttct gcgtttatat actagaagcg gcTTTACAGC TAGCTCAGTC
    3241 CTAGGGACTG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCGTTGCAAC TAAAGAGGAG
    3301 AAAGGTACCA TGGGGCATAA TCACAACCAC AATCACAATC ATAACCACAA TGGAGGTGAC
    3361 GATGACGATA AGGGTGGCCG CCAGGCCTGG ATCCCAGCCG GCCCCAAggc ccgatcgatg
    3421 ggaccaatgg ggcagcccct gcaagtgttg accctaaata tagaagatga gtatcggcta
    3481 catgagacct caaaagagcc agatgtttct ctagggtcca catggctgtc tgattttcct
    3541 caggcctggg cggaaaccgg gggcatggga ctggcagttc gccaagctcc tctgatcata
    3601 cctctgaaag caacctctac ccccgtgtcc ataaaacaat accccatgtc acaagaagcc
    3661 agactgggga tcaagcccca catacagaga ctgttggacc agggaatact ggtaccctgc
    3721 cagtccccct ggaacacgcc cctgctaccc gttaagaaac cagggactaa tgattatagg
    3781 cctgtccagg atctgagaga agtcaacaag cgggtggaag acatccaccc caccgtgccc
    3841 aacccttaca acctcttgag cgggctccca ccgtcccacc agtggtacac tgtgcttgat
    3901 ttaaaggatg cctttttctg cctgagactc caccccacca gtcagcctct cttcgccttt
    3961 gagtggagag atccagagat gggaatctca ggacaattga cctggaccag actcccacag
    4021 ggtttcaaaa acagtcccac cctgtttgat gaggcactgc acagagacct agcagacttc
    4081 cggatccagc acccagactt gatcctgcta cagtacgtgg atgacttact gctggccgcc
    4141 acttctgagc tagactgcca acaaggtact cgggccctgt tacaaaccct agggaacctc
    4201 gggtatcggg cctcggccaa gaaagcccaa atttgccaga aacaggtcaa gtatctgggg
    4261 tatcttctaa aagagggtca gagatggctg actgaggcca gaaaagagac tgtgatgggg
    4321 cagcctactc cgaagacccc tcgacaacta agggagttcc tagggacggc aggcttctgt
    4381 cgcctctgga tccctgggtt tgcagaaatg gcagccccct tgtaccctct caccaaaacg
    4441 gggactctgt ttaattgggg cccagaccaa caaaaggcct atcaagaaat caagcaagct
    4501 cttctaactg ccccagccct ggggttgcca gatttgacta agccctttga actctttgtc
    4561 gacgagaagc agggctacgc caaaggtgtc ctaacgcaaa aactgggacc ttggcgtcgg
    4621 ccggtggcct acctgtccaa aaagctagac ccagtagcag ctgggtggcc cccttgccta
    4681 cggatggtag cagccattgc cgtactgaca aaggatgcag gcaagctaac catgggacag
    4741 ccacttgtca ttctggcccc ccatgcagta gaggcacttg tcaaacaacc ccccgaccgc
    4801 tggctttcca acgcccggat gactcactat caggccttgc ttttggacac ggaccgggtc
    4861 cagttcggac cggtggtagc cctgaacccg gctacgctgc tcccactgcc tgaggaaggg
    4921 ctgcaacaca actgccttga tatcctggcc gaagcccacg gaacccgacc cgacctaacg
    4981 gaccagccgc tcccagacgc cgaccacacc tggtacacgg atggaagcag tctcttacaa
    5041 gagggacagc gtaaggcggg agctgcggtg accaccgaga ccgaggtaat ctgggctaaa
    5101 gccctgccag ccgggacatc cgctcagcgg gctgaactga tagcactcac ccaggcccta
    5161 aagatggcag aaggtaagaa gctaaatgtt tatactgata gccgttatgc ttttgctact
    5221 gcccatatcc atggagaaat atacagaagg cgtgggttgc tcacatcaga aggcaaagag
    5281 atcaaaaata aagacgagat ctttaaatga cgctgatagt gctagtgtag atcgctacta
    5341 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
    5401 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
    5461 tctgcgttta tatataataa atgtccagaA GGGccttcag gcatgcaagc ctctggaggc
    5521 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
    5581 cggtgaacgc tctcctgagt aggacaaatT ACTAGTAGCG GCCGCtgcag tccggcaaaa
    5641 aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt
    5701 atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    5761 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    5821 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    5881 tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    5941 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    6001 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    6061 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    6121 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    6181 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    6241 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    6301 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    6361 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    6421 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    6481 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    6541 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    6601 aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgagtcc cgtcaagtca
    6661 gcgtaatgct ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga
    6721 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
    6781 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
    6841 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
    6901 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
    6961 gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
    7021 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
    7081 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
    7141 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
    7201 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
    7261 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
    7321 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
    7381 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
    7441 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctggag caagacgttt
    7501 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
    7561 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
    7621 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagctc gagtgccact
    7681 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
    7741 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggAATTCGCG
    7801 GCCGCTTCTA GAGtttacag ctagctcagt cctagggact gtgctagcTG GAATTGTGAG
    7861 CGGATAACAA TTctatggac tatgtttCTG TCACCGGATG TGCTTTCCGG TCTGATGAGT
    7921 CCGTGAGGAC GAAAC

//`

sequences.seq_ZFc_codon_optimized_1 = {}; sequences.seq_ZFc_codon_optimized_1.varName = "seq_ZFc_codon_optimized_1"; sequences.seq_ZFc_codon_optimized_1.name = "ZFc (codon optimized) (1)"; sequences.seq_ZFc_codon_optimized_1.seqType = "linear"; sequences.seq_ZFc_codon_optimized_1.comp = true; sequences.seq_ZFc_codon_optimized_1.data = `LOCUS ZFc_(codon_optimized) 331 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    18..311
                    /label="ZFc with linker"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd

ORIGIN 

       1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA
      61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG
     121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA
     181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA
     241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC
     301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T

//`

sequences.seq_ZFc_codon_optimized = {}; sequences.seq_ZFc_codon_optimized.varName = "seq_ZFc_codon_optimized"; sequences.seq_ZFc_codon_optimized.name = "ZFc (codon optimized)"; sequences.seq_ZFc_codon_optimized.seqType = "linear"; sequences.seq_ZFc_codon_optimized.comp = true; sequences.seq_ZFc_codon_optimized.data = `LOCUS ZFc_(codon_optimized) 331 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers 

    misc_feature    18..311
                    /label="ZFc with linker"
                    /ApEinfo_revcolor=#ff9ccd
                    /ApEinfo_fwdcolor=#ff9ccd

ORIGIN 

       1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA
      61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG
     121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA
     181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA
     241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC
     301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T

//`