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| <br> | | <br> |
− | <b>Presented by iGEM_20 QHFZ-China</b><br> | + | <b>Presented by iGEM_20 QHFZ-China</b><br><br> |
− | <br>
| + | <b>Authors:</b><br> |
− | <div class="subsection quarter"> | + | <div class="subsection two_thirds"> |
− | <img src="https://static.igem.org/mediawiki/2020/7/72/T--QHFZ-China--Poster_section0_logo.png" alt="">
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− | </div>
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− | <div class="subsection three_quarters"> | + | |
− | <b>Authors:</b><br>
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| <b>Poster design: </b>Bohan Zhang<sup>1, 2</sup><br> | | <b>Poster design: </b>Bohan Zhang<sup>1, 2</sup><br> |
| <b>Wiki code: </b>Xinyuan Wang<sup>1, 2</sup><br> | | <b>Wiki code: </b>Xinyuan Wang<sup>1, 2</sup><br> |
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| <b>Proofread: </b>Celine Siu Ham Zhang<sup>1, 2</sup><br> | | <b>Proofread: </b>Celine Siu Ham Zhang<sup>1, 2</sup><br> |
| <b>Instructor: </b>Xing (Leaves) Zhang<sup>1, 3</sup> | | <b>Instructor: </b>Xing (Leaves) Zhang<sup>1, 3</sup> |
| + | </div> |
| + | <div class="subsection third"> |
| + | <img src="https://static.igem.org/mediawiki/2020/7/72/T--QHFZ-China--Poster_section0_logo.png" alt=""> |
| </div> | | </div> |
| <div class="clear"></div> | | <div class="clear"></div> |
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| 3. Beijing ZENO Co., Ltd., Beijing, China.<br><br><br> | | 3. Beijing ZENO Co., Ltd., Beijing, China.<br><br><br> |
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| <b>Abstract</b><br><br> | | <b>Abstract</b><br><br> |
| Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of <i>E. coli</i>. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.<br><br> | | Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of <i>E. coli</i>. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.<br><br> |
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| <img src="https://static.igem.org/mediawiki/2020/6/6b/T--QHFZ-China--Poster_section1.png"> | | <img src="https://static.igem.org/mediawiki/2020/6/6b/T--QHFZ-China--Poster_section1.png"> |
| <div class="info"> | | <div class="info"> |
− | <div class="title">标题</div> | + | <div class="title">Introduction & Inspiration</div> |
− | <div class="text">内容<b>加粗</b><i>斜体</i><sub>角标</sub><sup>角标</sup><br><br>sdadasdadasdsadadasdas | + | <div class="text"> |
| + | Our project is a continuation of the project last year. Last year, we made a portable household uric acid detector, whose core is a kind of genetically engineered bacteria. It would facilitate gout patients a lot. However, we realize that there are still some problems hindering its practical application. Among them, how to store the bacteria before use is an important issue. <br> |
| + | <img src="https://static.igem.org/mediawiki/2020/2/27/T--QHFZ-China--Poster_section1_1.png" alt=""><br> |
| + | Engineered bacteria should be stored with a long enough shelf life before use. They should stay alive, while their metabolism should be paused. In laboratory, we use ultralow temperature to achieve this. That is why -80℃ refrigerator is necessary for molecular biology lab. However, it is hard such professional equipment out of laboratories, especially in our daily life. In general, household refrigerators can only achieve about -20℃, which is insufficient. Besides, it is not ideal to use the refrigerator, which we stored food, to store bacteria. Indeed, many engineered bacteria are designed for our daily life. We interviewed 10 iGEM teams and most of them believed that a suitable method to solve the problem would benefit the practical application of their products. <br> |
| + | <img src="https://static.igem.org/mediawiki/2020/2/2b/T--QHFZ-China--Poster_section1_2.png" alt=""><br> |
| + | As a result, we decide to come up with a biopreservation method, which could help the storage of engineered bacteria get rid of professional equipment. |
| </div> | | </div> |
| </div> | | </div> |
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| <div class="text"> | | <div class="text"> |
| <br> | | <br> |
− | <b>Presented by iGEM_20 QHFZ-China</b><br> | + | <b>Presented by iGEM_20 QHFZ-China</b><br><br> |
− | <br>
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| <b>Authors:</b><br> | | <b>Authors:</b><br> |
− | <b>Poster design: </b>Bohan Zhang<sup>1, 2</sup><br> | + | <div class="subsection two_thirds"> |
− | <b>Wiki code: </b>Xinyuan Wang<sup>1, 2</sup><br>
| + | <b>Poster design: </b>Bohan Zhang<sup>1, 2</sup><br> |
− | <b>Content: </b>Yixian Yang<sup>1, 2</sup><br>
| + | <b>Wiki code: </b>Xinyuan Wang<sup>1, 2</sup><br> |
− | <b>Proofread: </b>Celine Siu Ham Zhang<sup>1, 2</sup><br>
| + | <b>Content: </b>Yixian Yang<sup>1, 2</sup><br> |
− | <b>Instructor: </b>Xing (Leaves) Zhang<sup>1, 3</sup><br><br>
| + | <b>Proofread: </b>Celine Siu Ham Zhang<sup>1, 2</sup><br> |
| + | <b>Instructor: </b>Xing (Leaves) Zhang<sup>1, 3</sup> |
| + | </div> |
| + | <div class="subsection third"> |
| + | <img src="https://static.igem.org/mediawiki/2020/7/72/T--QHFZ-China--Poster_section0_logo.png" alt=""> |
| + | </div> |
| + | <div class="clear"></div> |
| 1. iGEM team QHFZ-China 2020, China, Asia.<br> | | 1. iGEM team QHFZ-China 2020, China, Asia.<br> |
| 2. Tsinghua University High School, Beijing, China.<br> | | 2. Tsinghua University High School, Beijing, China.<br> |
| 3. Beijing ZENO Co., Ltd., Beijing, China.<br><br><br> | | 3. Beijing ZENO Co., Ltd., Beijing, China.<br><br><br> |
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| <b>Abstract</b><br><br> | | <b>Abstract</b><br><br> |
| Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of <i>E. coli</i>. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.<br><br> | | Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of <i>E. coli</i>. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.<br><br> |