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| <p style = "text-align: center;">Team UCL 2020</p> | | <p style = "text-align: center;">Team UCL 2020</p> |
− | <b>Team members</b><br> | + | <b>Student Team members</b><br> |
− | Olaide Ibiyemi, Juliette Champaud, Stefan Hristov, Pedro Lovatt Garcia, Daniel Castellano, Anna Su, Li Xu and Oliver Hernandez Fernandez | + | Olaide Ibiyemi, Juliette Champaud, Stefan Hristov, Pedro Lovatt Garcia, Daniel Castellano Garrido, Anna Su, Li Xu and Oliver Hernandez Fernandez |
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| <p>Figure 8. 1. Agent type over one a week, with time in a hours, showing active cells in blue, quiescent cells in orange, and dead cells in green. Each agent behaves as 125 cells. 2. Total current density generated by biofilm over one week</p> | | <p>Figure 8. 1. Agent type over one a week, with time in a hours, showing active cells in blue, quiescent cells in orange, and dead cells in green. Each agent behaves as 125 cells. 2. Total current density generated by biofilm over one week</p> |
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− | We observed a peak in the current density after about 48 hours at 1.2 A/m2. A steady state current density of approximately 0.0397 A/m2 was achieved lasting for over 9 days generating an average desalination rate of 0.623 L/m2/h. | + | We observed a peak in the current density after about 48 hours at 1.2 A/m2. A steady state current density of approximately 0.0397 A/m2 was achieved generating an average desalination rate of <b>0.623 L/m2/h</b> which is within the range of rates observed by Ramírez-Moreno et al. (12). |
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| + | For a lab-scale MDC with a 250 ml anode chamber and an anode surface area of 25cm2, 1.56 ml of water can be desalinated per hour. The system will have to be scaled up appropriately to desalinate enough water for irrigation. |
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| </div> | | </div> |
| </div> | | </div> |
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| <img src="https://static.igem.org/mediawiki/2020/1/1f/T--UCL--Poster_flowsheet_new.png"> | | <img src="https://static.igem.org/mediawiki/2020/1/1f/T--UCL--Poster_flowsheet_new.png"> |
− | <p>Figure 10. Flowsheet showing proposed implementation strategy of a desalination process</p> | + | <p>Figure 10. Flowsheet showing proposed implementation strategy of the desalination process</p><br> |
| + | As the FBA results showed that the co-culture and desalination have to be performed separately, the process has been split into 2 steps with an aerobic chamber for the co-culture and an anaerobic MDC chamber for electricity generation. After the co-culture, centrifugation and filtration are used to remove the cells from the outlet stream containing lactate which is then transferred to the anaerobic MDC.<br><br> |
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| + | From the CA, we found that the MDC will have to be scaled up to increase the surface area of the anode in order to desalinate enough water for irrigation. Through our research and communications with experts, we've proposed scaling up by stacking multiple MDCs or using a tubular MDC configuration. Though, this will have to be investigated further by next year's UCL iGEM team. |
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| </div> | | </div> |
| </div> | | </div> |
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| </div> | | </div> |
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− | <br><br><p><b>Team members</b></p> | + | <br><br><p><b>Student Team members</b></p> |
− | Olaide Ibiyemi, Juliette Champaud, Stefan Hristov, Pedro Lovatt Garcia, Daniel Castellano, Anna Su, Li Xu and Oliver Hernandez Fernandez | + | Olaide Ibiyemi, Juliette Champaud, Stefan Hristov, Pedro Lovatt Garcia, Daniel Castellano Garrido, Anna Su, Li Xu and Oliver Hernandez Fernandez |
| <br><br> | | <br><br> |
| <b>Supervisors and Instructors</b><br> | | <b>Supervisors and Instructors</b><br> |
− | Dr Stefanie Frank, Dr Kenth Gustafsson, Dr Chris Barnes, Dr Darren Nesbeth, Dr Rana Khalife, Alexander Van de Steen (Postgraduate Research student), Rory Gordon (Masters Research Student) and Neythen Treolar (Postgraduate Research Student). | + | Dr Stefanie Frank, Dr Kenth Gustafsson, Dr Chris Barnes, Dr Darren Nesbeth, Dr Rana Khalife, Alexander Van de Steen (Postgraduate Research student), Rory Gordon (Masters Research Student) and Neythen Treloar (Postgraduate Research Student). |
| <br><br> | | <br><br> |
| Thanks to everyone that shared their expertise and supported us throughout the project. | | Thanks to everyone that shared their expertise and supported us throughout the project. |
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| <li>Kane AL, Brutinel ED, Joo H, Maysonet R, VanDrisse CM, Kotloski NJ, et al. Formate Metabolism in Shewanella oneidensis Generates Proton Motive Force and Prevents Growth without an Electron Acceptor. Silhavy TJ, editor. J Bacteriol [Internet]. 2016 Apr;198(8):1337–46. Available from: https://jb.asm.org/content/198/8/1337</li> | | <li>Kane AL, Brutinel ED, Joo H, Maysonet R, VanDrisse CM, Kotloski NJ, et al. Formate Metabolism in Shewanella oneidensis Generates Proton Motive Force and Prevents Growth without an Electron Acceptor. Silhavy TJ, editor. J Bacteriol [Internet]. 2016 Apr;198(8):1337–46. Available from: https://jb.asm.org/content/198/8/1337</li> |
| <li>Luo S, Guo W, H. Nealson K, Feng X, He Z. 13C Pathway Analysis for the Role of Formate in Electricity Generation by Shewanella Oneidensis MR-1 Using Lactate in Microbial Fuel Cells. Sci Rep [Internet]. 2016 Aug;6(1):20941. Available from: http://www.nature.com/articles/srep20941</li> | | <li>Luo S, Guo W, H. Nealson K, Feng X, He Z. 13C Pathway Analysis for the Role of Formate in Electricity Generation by Shewanella Oneidensis MR-1 Using Lactate in Microbial Fuel Cells. Sci Rep [Internet]. 2016 Aug;6(1):20941. Available from: http://www.nature.com/articles/srep20941</li> |
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| <li>Knott BC, Erickson E, Allen MD, Gado JE, Graham R, Kearns FL, et al. Characterization and engineering of a two-enzyme system for plastics depolymerization. Proc Natl Acad Sci [Internet]. 2020 Oct 13 [cited 2020 Nov 9];117(41):25476–85. Available from: www.pnas.org/cgi/doi/10.1073/pnas.2006753117</li> | | <li>Knott BC, Erickson E, Allen MD, Gado JE, Graham R, Kearns FL, et al. Characterization and engineering of a two-enzyme system for plastics depolymerization. Proc Natl Acad Sci [Internet]. 2020 Oct 13 [cited 2020 Nov 9];117(41):25476–85. Available from: www.pnas.org/cgi/doi/10.1073/pnas.2006753117</li> |
| + | <li>Ramírez-Moreno M, Rodenas P, Aliaguilla M, Bosch-Jimenez P, Borràs E, Zamora P, et al. Comparative Performance of Microbial Desalination Cells Using Air Diffusion and Liquid Cathode Reactions: Study of the Salt Removal and Desalination Efficiency. Front Energy Res [Internet]. 2019 Dec 5 [cited 2020 Oct 24];7:135. Available from: https://www.frontiersin.org/article/10.3389/fenrg.2019.00135/full</li> |
| </ol> | | </ol> |
| </div> | | </div> |