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| <script src="https://2019.igem.org/Template:Calgary/DynamicSizeModelPage?action=raw&ctype=text/javascript"></script> | | <script src="https://2019.igem.org/Template:Calgary/DynamicSizeModelPage?action=raw&ctype=text/javascript"></script> |
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| <div class="text-area"> | | <div class="text-area"> |
| <div class="page-banner"> | | <div class="page-banner"> |
− | <h2 class="page-subtitle">Description</h2> | + | <h2 class="page-subtitle">Oviita</h2> |
− | <h1 class="page-title">Alberta Farmers and Oil Producers are Our Inspiration</h1> | + | <h1 class="page-title">Vitamin A Deficiency Solution</h1> |
| </div> | | </div> |
| </div> | | </div> |
− | </div> | + | |
− | | + | |
− | <div class="interface-group">
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− | <div class="content-area" id="textual-content">
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− | <div class="page">
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− | <div class="row"><div class="col-lg-2 col-md-2 col-sm-0 col-xs-0"></div><div class="col-lg-8 col-md-8 col-sm-12 col-xs-12">
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− | <div class="emphasis">
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− | Albertans are a tough breed.
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− | <br><br>
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− | We are a land of ranchers, roughnecks, foresters, and farmers.
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− | <br><br>
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− | We have tilled the earth through unforgiving prairie winters and we have thrived while doing it.
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− | <br><br>
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− | Our beautiful prairie landscape is painted yellow with budding canola.
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− | </div>
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− | <br><br>
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− | <div class="statement">
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− | We were inspired by the true grit of Albertan farmers who stand up to any challenge.
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− | This is iGEM Calgary's contribution.
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− | This is yOIL.
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− | </div></div><div class="col-lg-2 col-md-2 col-sm-0 col-xs-0"></div></div>
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− | </div>
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− | <div class="header-area">
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− | <h1>The Problem</h1>
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− | </div>
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− | <br><br>
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− | <img class ="center" src ="https://static.igem.org/mediawiki/2019/3/31/T--Calgary--field.jpg" style ="width:100%">
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− | <br><br>
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− | <img class ="center" src ="https://static.igem.org/mediawiki/2019/c/cb/T--Calgary--canola.png" style ="max-height:200px; width: auto;">
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− | <div class="subtitle">
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− | Canola oil is one of <b>Canada's leading exports</b>. In 2017, the Canadian grown canola contributed <b>$26.7 billion</b> to
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− | the Canadian economy each year, including more than <b>$250,000 jobs</b> and <b>$11.2 billion in wages</b>
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− | .
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− | </div>
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− | <br><br>
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− | <div class="statement">
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− | However, there is a threat.
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− | </div>
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− | <br><br>
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− | <div class="title">The Green Seed Problem</div>
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− | <img class ="center" src ="https://static.igem.org/mediawiki/2019/d/d6/T--Calgary--greenseed.png" style ="max-height:150px; width: auto;">
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− | <br><br>
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− | <div class="subtitle">
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− | As canola seeds mature, enzymes break down the green chlorophyll pigment resulting
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− | in a mature brown seed. The "green seed problem" arises when canola plants are exposed
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− | to frost, drought or other inclement weather before maturation is complete. The chlorophyll
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− | degradation pathway is suspended and the seed remains green.
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− | </div>
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− | <br><br>
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− | <div class = "statement">
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− | "Green is the difference between profit and loss."<br>
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− | <i>Craig Shand</i>
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− | </div>
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− | | + | |
− | <br><br>
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− | <div class="statement">
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− | What is so bad about that?
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− | </div>
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− | | + | |
− | <br><br>
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− | <div class="container">
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− | <div class="row">
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− | <div class="col-sm">
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− | Farmers lament the fact that their green canola seeds have to be sold
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− | at a discount price. Thus, a farmer like <a class="abody" href="https://2019.igem.org/Team:Calgary/Attributions">Craig Shand</a> is
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− | forced to go from <b>$10 per bushel to $7 per bushel</b> on a hard earned harvest.
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− | </div>
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− | <div class="col-sm">
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− | Excess chlorophyll in canola oil causes the oil to spoil faster, have a bitter taste, and a low smoke point. Downstream, oil crushers need to spend more money to remove chlorophyll from
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− | the canola oil in order to have a viable consumer product. This <b>process is costly, and oil is lost in the process</b>.
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− | </div>
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− | </div>
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− | </div>
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− | <br><br>
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− | | + | |
− | | + | |
− | <div class="statement">
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− | The green seed problem is a leech on the canola industry, stealing money out of the hands of farmers and oil producers.
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− | With the same grit and dedication of our hard working Albertan farmers, we developed yOIL.
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− | </div>
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− | <br><br>
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− | <div class="title">The Solution</div>
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− | <div class="row">
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− | <div class="col-lg-3 col-md-3 col-sm-0 col-xs-0"></div>
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− | <div class="col-lg-6 col-md-6 col-sm-12 col-xs-12">
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− | <br><br>
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− | <img class ="center" src ="https://static.igem.org/mediawiki/2019/2/21/T--Calgary--pipelineFull.png" style ="width:100%">
| + | |
− | <br><br>
| + | |
− | <p>
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− | yOIL is a multi-faceted attack on the green seed problem.
| + | |
− | </p>
| + | |
− | <p> The system
| + | |
− | utilizes
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− | <a class ="abody" href ="https://2019.igem.org/Team:Calgary/ChlorophyllExtraction">
| + | |
− | water-soluble-chlorophyll</a> binding proteins <a class = "abody" href = "https://2019.igem.org/Team:Calgary/EmulsifiedBindingProteinProcess">
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− | emulsified</a> in water droplets
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− | to remove chlorophyll molecules from oil.
| + | |
− | The captured chlorophyll will then be <a class = "abody" href = "https://2019.igem.org/Team:Calgary/RepurposingChlorophyll">
| + | |
− | repurposed</a> into pheophorbide, an experimental photosensitizer with potential as an
| + | |
− | <a class="abody" href="https://2019.igem.org/Team:Calgary/Anti-Fungal">anti-fungal</a> agent.
| + | |
− | </p>
| + | |
− | <p> To address ambiguities that arise when farmers take their seeds to be graded, our team developed
| + | |
− | a standardized seed grading platform, lovingly called <a class ="abody" href = "https://2019.igem.org/Team:Calgary/Modelling/StandardizedSeedGrading">"Mean Green Machine"
| + | |
− | </a> which automates the grading process. We took the Mean Green Machine (MGM) to <a class="abody" href="https://2019.igem.org/Team:Calgary/Human_Practices">The
| + | |
− | Canadian Grain Commission</a> where they gave us vital feedback to help move MGM closer to a fully-implemented product.
| + | |
− | </p>
| + | |
− | <p> Inclement weather is what causes green seed, and knowing the weather lets farmers
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− | make crucial decisions regarding their crop.
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− | We cannot control the weather, but we can create tools to predict it. After all, everyone could use more <a class ="abody" href="https://2019.igem.org/Team:Calgary/SunnyDays">Sunny Days</a>.
| + | |
− | Based on <dfn>Recurrent Neural Networks</dfn> and <dfn>Principal Component Analysis</dfn>, Sunny Days is a weather predictive algorithm capable of predicting the weather 180 days into the future
| + | |
− | with a mean absolute error of 2.0 degrees.
| + | |
− | </p>
| + | |
− |
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− |
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− |
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− |
| + | |
− | </div><div class="col-lg-3 col-md-3 col-sm-0 col-xs-0"></div>
| + | |
− | </div>
| + | |
− | <div class = "header-area">
| + | |
− | <img class ="center" src ="https://static.igem.org/mediawiki/2019/0/08/T--Calgary--yOIL-Background.png" style ="width:75%">
| + | |
− | </div>
| + | |
− | | + | |
− | | + | |
− | | + | |
− | <div class ="title">
| + | |
− | <h1>An All-Encompassing Solution to the Green Seed Problem</h1>
| + | |
− | </div>
| + | |
− | | + | |
− | <div class="subtitle">
| + | |
− | We have worked tirelessly to bring yOIL to fruition.
| + | |
− | <br>We have submitted <b>28 new parts</b> to
| + | |
− | the Biobrick registry, created <b>7 models</b> which informed our project design and held
| + | |
− | <b>over 35 meetings with stakeholders</b>, not to mention the innumerable number of hours in the
| + | |
− | lab (plus an extra 8 to travel to Boston).
| + | |
− | <br><br>
| + | |
− | After all is said and done, it is our privilege to present
| + | |
− | <br><br>
| + | |
− | </div>
| + | |
− | <img class ="center" src ="https://static.igem.org/mediawiki/2019/7/75/T--Calgary--yoilBlack.png" style ="width:20%">
| + | |
− | <div class="header-area">
| + | |
− |
| + | |
− | | + | |
− | </div>
| + | |
− | </div>
| + | |
− | </div>
| + | |
| </body> | | </body> |
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