Poster: 2020 Fudan
Make calcium supplement sustainable
- Student Leader: Shizheng Tian
- Student Members: Shuojin Liang, Mingwei Li, Ziyuan Wei, Jingqi Zhang, Tianhui Xu, Zhenru Zhang, Yiyang Yao, Yiyang Lu, Yuanyuan Liu, Emily Mo, Xiaohui Jiang, Xinran Li, Gaochen Zhang
- Advisors: Liang Cai, Yijie Pan, Yuchang Lee, Jingyi Hu
- Team PI: Daru Lu
- Affiliation: School of Life Sciences, Fudan University; with funding from YF Capital
In Asia, many old people suffer from insufficient calcium intake and related diseases such as osteoporosis. Considering that traditional calcium supplementation is not efficient and user-friendly enough, we intended to develop a kind of engineered bacteria based on E. coli that can colonize in the human intestine and secrete peptides to promote calcium absorption.
We introduced the quorum sensing system to colonize bacteria in the intestine. Once the bacteria have successfully colonized the intestinal tract, the luxPR promoter will be activated and a series of short peptides that can bind to calcium and promote calcium absorption of intestinal epithelial cells will be expressed. A kill switch is added accordingly for safety.
Our project provides a novel delivery method to provide calcium supplements sustainably. And we are actively seeking commercialization possibilities to expand our project impact, to reach more population, for the elderly's good health and well-being.
项目缘起，需要HP那四张关于骨质疏松的问卷图，数据不变 重画
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
铜奖满足，但不是罗列因为评委有一个列表给奖牌打分
- We have xxxx bala bala bala ba la ba la
- We have yyyy bala bala bala ba la ba la
- We have zzzz bala bala bala ba la ba la
- We have wxcv bala bala bala ba la ba la
银奖满足，但不是罗列因为评委有一个列表给奖牌打分
- We have xxxx bala bala bala ba la ba la
- We have yyyy bala bala bala ba la ba la
- We have zzzz bala bala bala ba la ba la
- We have wxcv bala bala bala ba la ba la
金奖满足，但不是罗列因为评委有一个列表给奖牌打分
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- We have yyyy bala bala bala ba la ba la
- We have zzzz bala bala bala ba la ba la
- We have wxcv bala bala bala ba la ba la
总结的话
描述我们项目如何贡献SDG3；为什么关怀老人，我们完成了什么，后来人继续做什么 bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
Our project aims to provide a holistic solution to the problem of osteoporosis among seniors. Our online general biology course Shengyin and senior-friendly textbooks, are also designed to educate the elderly on the importance of bone health.
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
描述我们项目如何贡献SDG4；我们为什么要教育，完成了什么，后来人继续做什么 bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
Our free online audio course The Voice Of Biology runs on multiple popular platforms with easy access to all age groups. We incorporated graphics to attract younger audiences and provided large font textbooks to accommodate seniors and those with hearing impairment. 上传两个ogg格式的文档
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la What is the problem your team is working to solve? How does it affect the world?
描述我们项目如何贡献SDG9；我们完成了什么，下一步将要做什么 bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
We created a thorough business plan for our project and further explored possible innovation in the industry by participating in youth innovation competition, pitching to investors and much more.
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la
bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la How are you going to solve the problem? Where did the idea come from?
CaAP stands for Calcium Absorption Peptide.
According to literature, we selected peptides @@@@GPAGPHGPVG, FDHIVY, YQEPVIAPKL, NDEELNK, and DHTKE. All of the first four peptides can form calcium-peptide complex through their Asp and Glu residues, and facilitate cell uptake. DHTKE uses its Asp, Glu and His residues to do the same. Basic part @@@@
We add a signal peptide to the N segment of CaAP to guide its secretion into the periplasmic space of E. coli. Because it is so small, it will then leak out of the cell wall into the outside environment. @@@@ 2 existing parts, 3 new; composite parts
We linked these peptides through Phe-Arg (FR) junctions. When CaAP is expressed and secreted by the engineered E. coli in the intestinal environment, CaAP could be further cleaved into functional oligopeptides in the presence of digestive enzymes (trypsin and chymotrypsin) in the intestinal lumen.
