Difference between revisions of "Team:Virginia/Poster"

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<div class="title">Manifold: Protein Shells with Encapsulated DNA Scaffolds for Increasing Efficiency of Biosynthetic Pathways</div>
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Presented by Team Virginia 2020
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<b>Monique De Freitas¹</b>, <b>Shawn Jin¹</b>, Evan Appleton², Swati Carr², Sonya Iverson², Traci Haddock³, and Douglas Densmore<sup>§</sup>
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<b>¹iGEM Student Team Member</b>, ²iGEM Team Mentor, ³iGEM Team Primary PI, <sup>§</sup>Faculty Sponsor,  Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA
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<b>Abstract</b>
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Our project has three aims: to introduce MoClo as an alternative assembly technique to BioBricks, to develop a standardized protocol for the characterization of genetic circuits using flow cytometry, and to share our MoClo Kit with the iGEM community. MoClo is an assembly technique developed by Weber et al., 2011, which involves a multi-way, one-pot digestion-ligation reaction, enabling faster and more efficient construction of genetic circuits. We converted a large subset of BioBricks from the Registry into MoClo Parts using PCR and cloning strategies. We are in the process of building and characterizing various genetic circuits using MoClo Parts, which we will compare to their BioBrick counterparts. A characterization workflow will be shared once this is complete. We also developed a data sheet using Clotho to be included in the Registry of Standard Biological Parts for each Part we characterized to easily share our data with the synthetic biology community.
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Revision as of 17:43, 9 November 2020

Manifold: Protein Shells with Encapsulated DNA Scaffolds for Increasing Efficiency of Biosynthetic Pathways
Presented by Team Virginia 2020

Monique De Freitas¹, Shawn Jin¹, Evan Appleton², Swati Carr², Sonya Iverson², Traci Haddock³, and Douglas Densmore§

¹iGEM Student Team Member, ²iGEM Team Mentor, ³iGEM Team Primary PI, §Faculty Sponsor, Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA

Abstract

Our project has three aims: to introduce MoClo as an alternative assembly technique to BioBricks, to develop a standardized protocol for the characterization of genetic circuits using flow cytometry, and to share our MoClo Kit with the iGEM community. MoClo is an assembly technique developed by Weber et al., 2011, which involves a multi-way, one-pot digestion-ligation reaction, enabling faster and more efficient construction of genetic circuits. We converted a large subset of BioBricks from the Registry into MoClo Parts using PCR and cloning strategies. We are in the process of building and characterizing various genetic circuits using MoClo Parts, which we will compare to their BioBrick counterparts. A characterization workflow will be shared once this is complete. We also developed a data sheet using Clotho to be included in the Registry of Standard Biological Parts for each Part we characterized to easily share our data with the synthetic biology community.
Introduction
Introduce your project and your team's goals.
Inspiration
What inspired your team? What motivated you to work on this particular project?
Problem
What is the problem your team is working to solve? How does it affect the world?
Idea
How are you going to solve the problem? Where did the idea come from?
Section 1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Section 2.1
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Section 2.2
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Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements
If not already cited in other sections of your poster, what literature sources did you reference on this poster? Who helped or advised you?