Presented by iGEM_20 QHFZ-China
Authors:
Poster design: Bohan Zhang1, 2
Wiki code: Xinyuan Wang1, 2
Content: Yixian Yang1, 2, Zhandong Jiao1, 2
Proofread: Bert Shan1, 2, Shao Chang1, 2
Instructor: Xing (Leaves) Zhang1, 3
Wiki code: Xinyuan Wang1, 2
Content: Yixian Yang1, 2, Zhandong Jiao1, 2
Proofread: Bert Shan1, 2, Shao Chang1, 2
Instructor: Xing (Leaves) Zhang1, 3
![](https://static.igem.org/mediawiki/2020/7/72/T--QHFZ-China--Poster_section0_logo.png)
2. Tsinghua University High School, Beijing, China.
3. Beijing ZENO Co., Ltd., Beijing, China.
Abstract
Various engineered bacteria displayed useful functions. However, these bacteria's storage usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. We could produce dry life-paused bacteria powder by introducing the TDPs as protectants into bacteria during lyophilization (freeze-drying). The powder can then be stored at room temperature for a long time without any professional equipment usage.
This year, we confirmed that certain TDPs could maintain the survival rate of E. coli. We also optimized the method by regulating the expression levels and combining different TDPs. Also, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and researchers are designing engineered bacteria for various application scenarios out of the laboratory, we hope our improvement in bio-preservation methods would promote their practical applications.
Team
![](https://static.igem.org/mediawiki/2020/1/14/T--QHFZ-China--Poster_section0_team.png)
This year, QHFZ-China is composed of 15 students. It is the second year for QHFZ-China to attend iGEM Jamboree.
Leaders: Yixian Yang, Zhandong Jiao
Members: Celine Siu Ham Zhang, Jiangshan Gao, Sizhe Duan, Marco Xue, Yeqi Wong, Xinyuan Wang, Bert Shan, James Haorui Wang, Junfei (Sophie) Li, Yiming Tang, Shao Chang, Siyuan Wang, Bohan Zhang
Primary PI: Shiqi Wang
Instructors: Xing (Leaves) Zhang, Tianze Zhu
Advisors: Zhimeng Xu, Jun Ma, Aiqi Zhao, Li TianHong, Luo Shaowei, Weizhao Chen
All of us welcome you to visit and critique our poster!