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| − | {{Poster}}
| + | https://static.igem.org/mediawiki/2020/b/b0/T--Paris_Bettencourt--Poster_EpiFlexLogo.png |
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| − | <html>
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| − | <style>
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| − | #section_title {
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| − | color: #ff0000;
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| − | background-color: #fff5efff;
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| − | text-align: center;
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| − | padding: 8px;
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| − | }
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| − | #section_text {
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| − | padding: 8px;
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| − | }
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| − | p {
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| − | color: #003366 !important;
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| − | text-align: justify;
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| − | }
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| − | .heading {
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| − | color: #ff0000 !important;
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| − | .padding-left {
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| − | padding-left: 8px;
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| − | .padding-right {
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| − | padding-right: 8px;
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| − | .logo {
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| − | width: 90% !important;
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| − | .logo-align{
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| − | text-align: center;
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| − | </style>
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| − | <div class="page_wrapper">
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| − | <div class="page_center">
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| − | <!-- <div style="padding: 20px 0px; width:95%; margin:auto; font-size:14px;">
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| − | <h1> Poster: Paris_Bettencourt </h1>
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| − | <br><br>
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| − | <h3> Poster Template </h3>
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| − | <p> This poster template will let you create an interactive poster! The poster is divided in two parts: the
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| − | visual overview on the left and the documentation on the right. The visual overview is broken down into
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| − | sections that the user can click on. When a user clicks on a poster section in the visual overview, the
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| − | documentation on the right will display the text and graphics associated with this section of the
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| − | poster. You can find the documentation on how to use this template, as well as an example here: <a
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| − | href="https://2020.igem.org/Competition/Deliverables/Poster">https://2020.igem.org/Competition/Deliverables/Poster</a>
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| − | </p>
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| − | | + | |
| − | </div> -->
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| − | | + | |
| − | <!---Place the content of your poster inside this div--->
| + | |
| − | <div class="poster_w content">
| + | |
| − | | + | |
| − | <!--This is the first row of the poster, it contains the title section --->
| + | |
| − | <!-- Poster title -->
| + | |
| − | <div class="row">
| + | |
| − | <!-- This creates a full width section with the id 'title' , since it will be the default section loaded, we add the class 'first_text' --->
| + | |
| − | <div class="section full first_text" id="title">
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| − | | + | |
| − | <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels -->
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| − | <img src="https://static.igem.org/mediawiki/2020/0/0f/T--Paris_Bettencourt--Poster_Title1.jpg">
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| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it since the first_text class is used above, this section will appear by default -->
| + | |
| − | <div class="info">
| + | |
| − | <!--Write the title of the section -->
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| − | <div class="title">SynDerma - The Skin Microbiome in the Spotlight: <br /> From Sampling to
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| − | Engineering
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| − | </div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
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| − | <p>Presented by Team Paris Bettencourt 2020</p>
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| − | <br />
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| − | <p><b>Amandine Maire<sup>1</sup>, Anu Susan Kurian<sup>1</sup>, Chetan Kumar
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| − | Velumurugan<sup>1</sup>, Nicolas Levrier<sup>1</sup>, Nikola Zarevski<sup>1</sup>,
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| − | Valerie March<sup>1</sup>, Xavier Olessa-Daragon<sup>1</sup>, Ariel
| + | |
| − | Lindner<sup>2</sup>, Jake Wintermute<sup>2</sup>, Radoslaw Ejsmont<sup>2</sup>,
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| − | Alexis Casas<sup>2</sup>, Darshak Bhatt<sup>2</sup></b></p>
| + | |
| − | <br />
| + | |
| − | <p><b><sup>1</sup>Student Team Member</b>, <sup>2</sup>Team Mentor</p>
| + | |
| − | <br />
| + | |
| − | <b>Abstract</b>
| + | |
| − | <p></p>
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| − | </div>
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| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | | + | |
| − | | + | |
| − | <!--This is the second row of the poster, it contains the introduction section --->
| + | |
| − | <!-- Objectives -->
| + | |
| − | <div class="row">
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| − | <!-- This creates a full width section with the id 'objective' --->
| + | |
| − | <div class="section full" id="objective">
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| − | <!--Here is the image used for the full width section, the size is: 1200 x 200 pixels -->
| + | |
| − | <img src="https://static.igem.org/mediawiki/2020/9/90/T--Paris_Bettencourt--Poster_Objectives1.jpg">
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| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
| + | |
| − | <div class="info">
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| − | <!--Write the title of the section -->
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| − | <div class="title"> Objectives </div>
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| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
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| − | <!-- <p><b>Objectives 1.</b></p> -->
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| − | <p><b class="heading">1. Sample and Sequence the Human Skin Microbiome</b></p>
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| − | <p>Our first objective, pursued as part of the Quaranskin project, is to study the impact of
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| − | environmental and behavioral factors on the skin microbiome and to provide a new
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| − | database to study its composition in the context of reduced social and environmental
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| − | interactions.
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| − | </p>
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| − | <br />
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| − | <!-- <p><b>Objectives 2.</b></p> -->
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| − | <p><b class="heading">2. Design tools to engineer the bacterium <i>S. epidermidis</i></b>
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| − | </p>
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| − | <p>Our second objective is to make S. epidermidis an efficient synthetic biology chassis
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| − | that can be used to monitor the population dynamics of the skin microbiome. This in
| + | |
| − | order to maintain its equilibrium and to avoid pathologies induced by dysbiosis.
| + | |
| − | </p>
| + | |
| − | </div>
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| − | </div>
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| − | </div>
| + | |
| − | </div>
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| − | | + | |
| − | | + | |
| − | <!--This is the third row of the poster, it contains the inspiration, problem and idea sections --->
| + | |
| − | <!-- Inspiration, Challenge, Response -->
| + | |
| − | <div class="row">
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| − | <!-- This creates a third width section with the id 'inspiration' --->
| + | |
| − | <!-- Inspiration -->
| + | |
| − | <div class="section third" id="inspiration">
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| − | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels -->
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| − | <img src="https://static.igem.org/mediawiki/2020/1/18/T--Paris_Bettencourt--Poster_Inspiration31.jpg">
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| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
| + | |
| − | <div class="info">
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| − | | + | |
| − | <!--Write the title of the section -->
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| − | <div class="title"> Inspiration </div>
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| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
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| − | <p>In the context of COVID-19 pandemic, we were, as most of the other iGEM teams confined at
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| − | home.</p>
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| − | | + | |
| − | <div class="subsection third">
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| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/e/e5/T--Paris_Bettencourt--Poster_Inspiration1.png">
| + | |
| − | </div>
| + | |
| − | <div class="subsection two_thirds">
| + | |
| − | <br />
| + | |
| − | <br />
| + | |
| − | <p class="padding-left">As this situation was unprecedented, we were wondering if the
| + | |
| − | lockdown could have an
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| − | impact on human health and more specifically on the composition of our skin
| + | |
| − | microbiome.
| + | |
| − | </p>
| + | |
| − | </div>
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| − | | + | |
| − | <div class="clear"></div>
| + | |
| − | <br />
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| − | | + | |
| − | <div class="subsection two_thirds">
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| − | <p class="padding-right">We met some dermatologists as Stéphanie Leclerc-Mercier. She
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| − | pointed out the fact
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| − | that the new sanitary measures and hygiene habits imposed by the pandemic were a
| + | |
| − | real issue for people with eczema symptoms since they cannot wash their hands as
| + | |
| − | often as healthy people. This discussion drew our interest for dysbiosis induced
| + | |
| − | pathologies and ways to treat them using synthetic biology.
| + | |
| − | </p>
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| − | </div>
| + | |
| − | <div class="subsection third">
| + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/0/03/T--Paris_Bettencourt--Poster_Inspiration2.png">
| + | |
| − | </div>
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| − | | + | |
| − | <div class="clear"></div>
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| − | <br />
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| − | | + | |
| − | <div class="subsection third">
| + | |
| − | <img
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| − | src="https://static.igem.org/mediawiki/2020/6/63/T--Paris_Bettencourt--Poster_Inspiration3.png">
| + | |
| − | </div>
| + | |
| − | <div class="subsection two_thirds">
| + | |
| − | <br />
| + | |
| − | <br />
| + | |
| − | <p class="padding-left">Our team personal values led us to create a project who enters
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| − | in
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| − | the framework of
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| − | Open and Citizen Science. Especially during the lockdown which forced us to be
| + | |
| − | physically isolated of each other, we wanted to find a way to connect people through
| + | |
| − | science.
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | | + | |
| − | | + | |
| − | <!-- This creates a third width section with the id 'challenge' --->
| + | |
| − | <!-- Challenge -->
| + | |
| − | <div class="section third" id="challenges">
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| − | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels -->
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| − | <img src="https://static.igem.org/mediawiki/2020/7/7e/T--Paris_Bettencourt--Poster_Challenges32.jpg">
| + | |
| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
| + | |
| − | <div class="info">
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| − | | + | |
| − | <!--Write the title of the section -->
| + | |
| − | <div class="title"> Challenges </div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
| + | |
| − | <p><b class="heading">1. Study the Skin Microbiome</b></p>
| + | |
| − | <p>Human skin microbiome sampling is a challenge in itself since it implies reaching out to
| + | |
| − | a
| + | |
| − | large cohort and collecting human being derived samples. In the context of a
| + | |
| − | pandemic other constraints are added because of social distancing measures.</p>
| + | |
| − | | + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/8/89/T--Paris_Bettencourt--Poster_SkinMicrobiomeStudy.png">
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| − | | + | |
| − | <p><b class="heading">2. Engineer the Skin Microbiome</b></p>
| + | |
| − | <ul>
| + | |
| − | <li><i>Staphylococcus epidermidis</i> is not used in synthetic biology and no tools are
| + | |
| − | currently available to engineer it</li>
| + | |
| − | <li>Thw two main challenges in using <i>S. epidermidis</i> in
| + | |
| − | synthetic biology are:</li>
| + | |
| − | </ul>
| + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/1/1f/T--Paris_Bettencourt--Poster_SkinMicrobiomeEngineering.png">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <!-- This creates a third width section with the id 'response' --->
| + | |
| − | <!-- Response -->
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| − | <div class="section third" id="responses">
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| − | <!--Here is the image used for the third width section, the size is: 400 x 200 pixels -->
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| − | <img src="https://static.igem.org/mediawiki/2020/5/5b/T--Paris_Bettencourt--Poster_Responses33.jpg">
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| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
| + | |
| − | <div class="info">
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| − | | + | |
| − | <!--Write the title of the section -->
| + | |
| − | <div class="title"> Responses </div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
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| − | <p><b class="heading">1. Quaranskin</b></p>
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| − | <p>Quaranskin is a study involving the collection of skin microbiome samples during the
| + | |
| − | COVID-19 pandemic and
| + | |
| − | observing the impact of environmental factors on the skin microbiome.
| + | |
| − | </p>
| + | |
| − | <ul>
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| − | <li>We took <b>ethical considerations</b> very seriously by submitting our project to
| + | |
| − | the French national ethics committee in order to obtain their approval before
| + | |
| − | starting the study
| + | |
| − | </li>
| + | |
| − | <li>In order to include <b>citizen participation</b>, we designed a smapling kit
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| − | containing all the material required for sampling the skin microbiome and set up
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| − | mailing system to ship and collect back the samples from participants
| + | |
| − | </li>
| + | |
| − | <li>We are building an <b>Open Database</b> of skin microbiome during the COVID-19
| + | |
| − | pandemic
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | <br />
| + | |
| − | <p><b class="heading">2. EpiFlex, EpiGlow, EpiGrow</b></p>
| + | |
| − | <p>These three projects aims to develop tools to make S. epidermidis a good chassis for
| + | |
| − | Synthetic biology.
| + | |
| − | </p>
| + | |
| − | <ul>
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| − | <li><b>EpiFlex</b> is a MoClo tool kit for <i>S. epidermidis</i> </li>
| + | |
| − | <li><b>EpiGlow</b> is the proof of concept of EpiFlex that aims to optimize
| + | |
| − | transformation protocol <i>S. epidermidis</i></li>
| + | |
| − | <li><b>EpiGrow</b> is the optimization of <i>S. epidermidis</i> growth</i> </li>
| + | |
| − | </ul>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | </div>
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| − | | + | |
| − | | + | |
| − | <!--This is the fourth row of the poster, it contains the Skin Microbiome section --->
| + | |
| − | <!-- Skin Microbiome -->
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| − | <div class="row">
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| − | <!-- This creates a full width section with the id 'skin_microbiome' --->
| + | |
| − | <div class="section full" id="skin_microbiome">
| + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/a/a9/T--Paris_Bettencourt--Poster_TheSkinMicrobiome.jpg">
| + | |
| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
| + | |
| − | <div class="info">
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| − | <!--Write the title of the section -->
| + | |
| − | <div class="title">The Skin Microbiome</div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
| + | |
| − | <p><b class="heading">What is the Skin Microbiome?</b></p>
| + | |
| − | <p>The human skin microbiome is a vast and very large ecosystem of microorganisms that
| + | |
| − | occupy the human skin at the level of the hypodermis, dermis, and epidermis. It is very
| + | |
| − | numerous and very diverse, a real petri dish hidden in plain sight.
| + | |
| − | </p>
| + | |
| − | <br />
| + | |
| − | <p><b class="heading">How the Skin Microbiome plays a role in the skin health?</b></p>
| + | |
| − | <p>The human skin microbiome is very tightly linked to the health of its host in numerous
| + | |
| − | ways. First of all the microorganisms of the skin microbiome prevent the colonization of
| + | |
| − | the skin by pathological microorganisms, and help to train the immune system to make it
| + | |
| − | more prepared to face pathogens. It also directly involved in various pathologies, like
| + | |
| − | atopic dermatitis, where its overall diversity is reduced and Staphylococcus aureus is
| + | |
| − | over represented.
| + | |
| − | </p>
| + | |
| − | <br />
| + | |
| − | <p><b class="heading">Why studying the composition of the Skin Microbiome?</b></p>
| + | |
| − | <p>
| + | |
| − | <ul>
| + | |
| − | <li>Most of the previous studies which lead to understand the skin microbiome by
| + | |
| − | sampling human volunteers were based on North American subjects. Form these results,
| + | |
| − | current evidence suggests a greater microbial diversity to be a beneficial trait,
| + | |
| − | however there is a need for greater diversity in the participant pools from which
| + | |
| − | this data is derived. Thus, a study observing human skin microbiome among a European
| + | |
| − | population would give new data increasing our knowledge of the skin microbiome.
| + | |
| − | </li>
| + | |
| − | <li>Besides the current global environment wherein a significant number of people are
| + | |
| − | minimizing their interactions with other people is a good opportunity to observe
| + | |
| − | environmental impact on skin microbiome in a more simplified model.
| + | |
| − | </li>
| + | |
| − | <li>Also, by taking samples from the skin microbiome during the COVID-19 pandemic, we
| + | |
| − | will have preserved evidence of the influence of the social restrictions induced by
| + | |
| − | the pandemic on the skin microbiome.
| + | |
| − | </li>
| + | |
| − | </ul>
| + | |
| − | </p>
| + | |
| − | <br />
| + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/8/80/T--Paris_Bettencourt--Poster_SkinMicrobiomeIllustration.png">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <!--This is the fifth row of the poster, it contains the Quaranskin and Implementation section --->
| + | |
| − | <!-- Quaranskin, Implementation -->
| + | |
| − | <div class="row">
| + | |
| − | <!-- This creates a full width section with the id 'quaranskin' --->
| + | |
| − | <!-- Quaranskin -->
| + | |
| − | <div class="section two_thirds" id="quaranskin">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2020/5/5b/T--Paris_Bettencourt--Poster_Quaranskin51.jpg">
| + | |
| − | | + | |
| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
| + | |
| − | <div class="info">
| + | |
| − | <!--Write the title of the section -->
| + | |
| − | <div class="title">Quaranskin</div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
| + | |
| − | <p>Quaranskin, a combination of quarantine and skin, is a project based on the collection
| + | |
| − | and analysis of skin microbiome samples, collected from participants across Europe.
| + | |
| − | </p>
| + | |
| − | <p>We aim to understand if there are correlations between behavioral characteristics that
| + | |
| − | involve activity, hygiene and human interaction, and the diversity and composition of
| + | |
| − | skin microbes at four body sites.
| + | |
| − | </p>
| + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/6/6e/T--Paris_Bettencourt--Poster_QuaranskinStudy1.png">
| + | |
| − | <br />
| + | |
| − | <p><b class="heading">Study Pipeline</b></p>
| + | |
| − | <br />
| + | |
| − | <img
| + | |
| − | src="https://static.igem.org/mediawiki/2020/1/11/T--Paris_Bettencourt--Poster_QuaranskinStudy2.png">
| + | |
| − | <p><b>Participants recruitment</b></p>
| + | |
| − | <p>Emails are sent to all the members of our institution, the CRI, and all the european
| + | |
| − | iGEMers, by focusing on people form countries where confinement or social distanciation
| + | |
| − | are set up. The total number of participants expected is 70.
| + | |
| − | </p>
| + | |
| − | <p><b>Participants enrollment</b></p>
| + | |
| − | <p>To be involved in the study each participants has to fill in a participation form and
| + | |
| − | link to it a signed and dated consent form.
| + | |
| − | </p>
| + | |
| − | <p>A link is sent to them allowing them to create a account on the OpenHumans platform in
| + | |
| − | order to furnish them an ID code which we use to keep anonymity
| + | |
| − | </p>
| + | |
| − | <p><b>Participants action</b></p>
| + | |
| − | <p>Once they are officially enroll in the study, we send to the participants by mail a kit
| + | |
| − | containing all tools needed to sample microbiome from 4 body sites and to send them back
| + | |
| − | to us, and by email a link to answer an online questionnaire asking questions covering 4
| + | |
| − | main topics : The intrinsic characteristic (age, sexe, nationality...), the hygiene
| + | |
| − | habits, the level of confinement and the skin disorder appeared since February 2020.
| + | |
| − | </p>
| + | |
| − | <p><b>Microbiome sequencing</b></p>
| + | |
| − | <p>Once we receive the microbiome samples we send them directly to an external company,
| + | |
| − | Genewiz. They’ll extract bacterial DNA from the samples, amplify the V3-V4 regions of
| + | |
| − | the 16S RNA gene, and then sequence the amplicons.
| + | |
| − | </p>
| + | |
| − | <p><b>Statistical analysis</b></p>
| + | |
| − | <p>After having analyzed the composition and diversity of each microbiome we’ll link these
| + | |
| − | results to the answer to the questionnaire to finally find some correlations between
| + | |
| − | some microbiome compositions and some characteristics of the lifestyle or some skin
| + | |
| − | disorder
| + | |
| − | </p>
| + | |
| − | <br />
| + | |
| − | | + | |
| − | <p><b class="heading">Data Analysis</b></p>
| + | |
| − | <p><b>1. Diversity analysis by index of hygiene, personal information, level of restriction
| + | |
| − | </b></p>
| + | |
| − | <p>The microbiomes in the generated database are grouped by index value in each of the 3
| + | |
| − | categories. By setting two indices, we can study the impact of the third element on the
| + | |
| − | diversity of the microbiome
| + | |
| − | </p>
| + | |
| − | <p><b>2. Environmental factors which influence proportion of Staphylococcus
| + | |
| − | </b></p>
| + | |
| − | <p>We define a proportion threshold, then we identify all the people who present a
| + | |
| − | population of Staphylococcus beyond this threshold. We finally look for parameters
| + | |
| − | common to these individuals,
| + | |
| − | </p>
| + | |
| − | <p><b>3. Researching existing microbiome composition among our data set
| + | |
| − | </b></p>
| + | |
| − | <p>We want to compare a typical composition of eczematic microbiome found in literature,
| + | |
| − | with our data
| + | |
| − | </p>
| + | |
| − | <p><b>4. Analysis of data based on common symptoms
| + | |
| − | </b></p>
| + | |
| − | <p>When a significant number of people present the same symptom, independently of the
| + | |
| − | environment and lifestyle, we want to make a synthesis of the typical composition of the
| + | |
| − | microbiome for this symptom.
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | <div class="section third" id="Implementation">
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| − | <div class="title">Implementation</div>
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| + | |
| − | <div class="text">
| + | |
| − | <p><b class="heading">Ethical consideration</b></p>
| + | |
| − | <p>As Quaranskin is a collection of data extracted from human derived samples (skin
| + | |
| − | microbiome) and personal information (answer to the questionnaire), it’s important to
| + | |
| − | learn about the ethical rules that govern research involving human being in Europe in
| + | |
| − | order to respect the protection of the participants in our study.
| + | |
| − | </p>
| + | |
| − | <p>In France, Research Involving Human Being (RIPH) has to be approved and framed by the
| + | |
| − | Committees for the Protection of Persons (CPP).
| + | |
| − | </p>
| + | |
| − | <p>We have submitted to the ethics committee a complete file presenting our study in detail.
| + | |
| − | This file consisted mainly of a research protocol and all the information documents for
| + | |
| − | the study participants. Even though we already recruit participant and set up all the
| + | |
| − | logistic aspect of the study, we are still waiting for their approval to collect and
| + | |
| − | analyse samples.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <p><b class="heading">Contribution to the scientific community</b></p>
| + | |
| − | <ol>
| + | |
| − | <li>Increase the knowledge we have about the microbiome and more precisely about the
| + | |
| − | external factors that can impact it.
| + | |
| − | </li>
| + | |
| − | <li>Create an open database of skin microbiome from people in the context of a pandemic.
| + | |
| − | </li>
| + | |
| − | <li>Develop Science@Home by providing pipeline and protocols useful for future
| + | |
| − | microbiome studies based on citizen science
| + | |
| − | </li>
| + | |
| − | <li>Instruct participants about their microbiome by sending them back their microbiome
| + | |
| − | profile after analysis.
| + | |
| − | </li>
| + | |
| − | </ol>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
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| − | <!-- Engineer -->
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| − | <div class="row">
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| − | <div class="section full" id="engineer">
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| − | <img src="https://static.igem.org/mediawiki/2020/4/40/T--Paris_Bettencourt--Poster_Engineering1.jpg">
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| − | <div class="info">
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| − | <div class="title"> Engineering </div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
| + | |
| − | <p>Biological engineering allowed us to develop solutions for our objectives.</p>
| + | |
| − | <p><b class="heading">Purpose</b></p>
| + | |
| − | <p>We aim to sense and modulate population dynamics of the skin microbiome in order to help
| + | |
| − | maintain its equilibrium and avoid dysbiosis induced pathologies. This is where we
| + | |
| − | envision the role of synthetic biology in the probiotic arena.
| + | |
| − | </p>
| + | |
| − | <p>More precisely, we thought about engineering <i>S. epidermidis</i> in order to control
| + | |
| − | overgrowth of <i>S. aureus</i> which can induce eczema.
| + | |
| − | </p>
| + | |
| − | <p><b class="heading"><i>S. epidermidis</i></b></p>
| + | |
| − | <p><i>S. epidermidis</i> represents approximately 90% of the aerobic skin flora, and about 5% of
| + | |
| − | the overall skin microbiome species. It is a non-motile gram-positive coccus, and a
| + | |
| − | facultative anaerobe. Its mainly mutualistic behavior makes it a very good candidate to
| + | |
| − | become a vector for sensors or ways to modulate the skin microbiome.
| + | |
| − | </p>
| + | |
| − | <p><b class="heading">Research Focus</b></p>
| + | |
| − | <p>Three essential stages that we want to optimize to help engineer <i>S. epidermidis</i>:
| + | |
| − | </p>
| + | |
| − | <p></p>
| + | |
| − | <ol>
| + | |
| − | <li>Grow</li>
| + | |
| − | <li>Transform</li>
| + | |
| − | <li>Clone</li>
| + | |
| − | </ol>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
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| − | <div class="row">
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| − | <img src="https://static.igem.org/mediawiki/2020/9/91/T--Paris_Bettencourt--Poster_EpiFlex71.jpg">
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| − | <!--Info: Place the title and text for this section, it will appear when the user clicks on it-->
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| + | |
| − | <div class="title">EpiFlex</div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
| + | |
| − | <div class="subsection half">
| + | |
| − | <p>The bacterium Staphylococcus epidermidis has only few tools available for efficient expression of recombinant DNA and genetic engineering. This generated the idea to develop a MoClo toolkit for S.epidermidis, the EpiFlex toolkit.
| + | |
| − | </p>
| + | |
| − | <p>EpiFlex aims to make the bacterium a chassis for synthetic biology.
| + | |
| − | </p>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="subsection half logo-align">
| + | |
| − | <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_EpiGlow72.jpg" class="logo">
| + | |
| − | </div>
| + | |
| − |
| + | |
| − | <div class="clear"></div>
| + | |
| − | <br />
| + | |
| − | | + | |
| − | <p><b class="heading">MoClo Toolkit</b></p>
| + | |
| − | <p>The MoClo is a modular cloning method based on Golden Gate assembly. EpiFlex is a Moclo toolkit developed with parts that function specifically in S.epidermidis.
| + | |
| − | </p>
| + | |
| − | <p><b class="heading">The MoClo Concept</b></p>
| + | |
| − | <img src="https://static.igem.org/mediawiki/2020/6/6b/T--Paris_Bettencourt--Poster_MoCloConcept.png">
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Paris_Bettencourt--Poster_EpiGlow72.jpg">
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| − | <div class="title"> Section 2 </div>
| + | |
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| − | <div class="text"> Use this section to explain whatever you would like! Suggestions: Safety,
| + | |
| − | Human Practices, Measurement, etc. </div>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | <div class="title"> Section 3 </div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text"> Use this section to explain whatever you would like! Suggestions: Safety,
| + | |
| − | Human Practices, Measurement, etc. </div>
| + | |
| − | </div>
| + | |
| − | </div>
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| − | </div>
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| − | <div class="title"> Results</div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text"> What did your team achieve? What do you plan to work on moving forward?
| + | |
| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="title">References and Acknowledgements</div>
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| + | |
| − | <div class="text">If not already cited in other sections of your poster, what literature sources
| + | |
| − | did you reference on this poster? Who helped or advised you? </div>
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| − | </div>
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| − | </div>
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| − | <img src="https://static.igem.org/mediawiki/2020/2/24/T--Paris_Bettencourt--Poster_iGEMExperience1.jpg">
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| + | |
| − | <div class="title"> Our iGEM Experience</div>
| + | |
| − | <!--Write the text explaining this section -->
| + | |
| − | <div class="text">
| + | |
| − | <!-- <img src="https://static.igem.org/mediawiki/2020/f/f8/T--Paris_Bettencourt--Poster_iGEMExperienceLogo.jpg"
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| − | class="logo">
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| − | <div class="clear"></div>
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| − | <br /> -->
| + | |
| − | | + | |
| − | <p>iGEM 2020 was a unique opportunity to meet other aspiring and motivated researchers that
| + | |
| − | helped broaden our knowledge on both human and scientific topics! We truly enjoyed our
| + | |
| − | experience while attending the virtual meetings and discussing on various topics with
| + | |
| − | iGEMers across the world.
| + | |
| − | </p>
| + | |
| − | <p><b class="heading">A Chit-Chat around the Skin Microbiome</b></p>
| + | |
| − | <p>In early June, our team enjoyed hosting a ChitChat session to discuss on the subject of
| + | |
| − | the Skin Microbiome. Several iGEM teams joined us in this session and provided us with
| + | |
| − | the opportunity in sharing our experience on this topic with them.
| + | |
| − | </p>
| + | |
| − | <p><b class="heading">iGEMeetParis: A Parisian Virtual Meetup</b></p>
| + | |
| − | <p>During the first weekend of September, we hosted our own virtual meetup, together with
| + | |
| − | the other Parisian teams. It took two months of preparation to set up an amazing weekend
| + | |
| − | lined up with workshops, hackathons, discussions, pitch presentations and other cool
| + | |
| − | social events!
| + | |
| − | </p>
| + | |
| − | <p><b class="heading">Cité des Sciences</b></p>
| + | |
| − | <p>We had the wonderful opportunity to display our project at the Cité des Sciences Annual
| + | |
| − | Science Fair in Paris where we shared our scientific communication with the general
| + | |
| − | public.
| + | |
| − | </p>
| + | |
| − | </div>
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| − | </div>
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| − | </div>
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| − | </div>
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| − | <div class="text">
| + | |
| − | <p><b class="heading">Acknowledgements</b></p>
| + | |
| − | <p>Valérie Antonio</p>
| + | |
| − | <p>Mad Price Ball</p>
| + | |
| − | <p>Céline Couteau</p>
| + | |
| − | <p>Paulina Ejsmont</p>
| + | |
| − | <p>Stéphanie Leclerc-Mercier</p>
| + | |
| − | <p>Valérie Lerouyer</p>
| + | |
| − | <p>Hamid Mebrouki</p>
| + | |
| − | <p>Piers Millett</p>
| + | |
| − | <p>Ian Monk</p>
| + | |
| − | <p>Julia Oh</p>
| + | |
| − | <p>Alexandre Singier</p>
| + | |
| − | <p>Jean-Christophe Thalabard</p>
| + | |
| − | <p>Bastian Greshake Tzovaras</p>
| + | |
| − | <br/>
| + | |
| − |
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| − | <p><b class="heading">Sponsors</b></p>
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| − | src="https://static.igem.org/mediawiki/2020/a/a3/T--Paris_Bettencourt--Poster_FondationBettencourtSchueller.png" class="logo">
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| − | </div>
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| − | src="https://static.igem.org/mediawiki/2020/4/47/T--Paris_Bettencourt--Poster_Geneious.png" class="logo">
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| − | <!--This is where the selected content will be displayed - Don't change this section!-->
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| − | <div class="poster_w display">
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| − | <h3 id="section_title"></h3>
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| − | <p id="section_text"></p>
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| − | </div>
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| − | </div>
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| − | </div>
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