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The lack of a versatile and reliable way to improve metabolic flux channeling, pathway orthogonality, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of bacterial microcompartments (BMCs) with encapsulated dsDNA scaffolds that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as zinc-finger fusion proteins. Here we deliver the designs for an E. coli cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis.
 
The lack of a versatile and reliable way to improve metabolic flux channeling, pathway orthogonality, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of bacterial microcompartments (BMCs) with encapsulated dsDNA scaffolds that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as zinc-finger fusion proteins. Here we deliver the designs for an E. coli cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis.
 
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<div class="text">The field of synthetic biology has seen great success in utilizing bacteria and yeast to produce chemicals of medical and industrial value, but these methods still have the potential to be improved. In many cases, prokaryotes are structurally simpler and generally easier to engineer than eukaryotes, but do not possess a simple way to compartmentalize reaction pathways like eukaryotic organelles do. We strove to combine the ease of use of prokaryotes with the compartmentalization capability of eukaryotes by developing a method to synthetically isolate a simple biosynthetic pathway - resveratrol synthesis - within a prokaryotic system.</div>
 
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Revision as of 17:53, 9 November 2020

Manifold: Protein Shells with Encapsulated DNA Scaffolds for Increasing Efficiency of Biosynthetic Pathways
Presented by Team Virginia 2020

Team: J. Ball, V. Gutierrez, C. Haws, A. Kola, S. Link, C. Marino, E. Micklovic, D. Patel, J. Polzin, A. Pradhan, P. Revelli

Advisors: K. G. Kozminski, J. A. Papin

Abstract:
The lack of a versatile and reliable way to improve metabolic flux channeling, pathway orthogonality, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of bacterial microcompartments (BMCs) with encapsulated dsDNA scaffolds that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as zinc-finger fusion proteins. Here we deliver the designs for an E. coli cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis.
Inspiration
The field of synthetic biology has seen great success in utilizing bacteria and yeast to produce chemicals of medical and industrial value, but these methods still have the potential to be improved. In many cases, prokaryotes are structurally simpler and generally easier to engineer than eukaryotes, but do not possess a simple way to compartmentalize reaction pathways like eukaryotic organelles do. We strove to combine the ease of use of prokaryotes with the compartmentalization capability of eukaryotes by developing a method to synthetically isolate a simple biosynthetic pathway - resveratrol synthesis - within a prokaryotic system.
Inspiration
What inspired your team? What motivated you to work on this particular project?
Problem
What is the problem your team is working to solve? How does it affect the world?
Idea
How are you going to solve the problem? Where did the idea come from?
Section 1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Section 2.1
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Section 2.2
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Section 3
Use this section to explain whatever you would like! Suggestions: Safety, Human Practices, Measurement, etc.
Results
What did your team achieve? What do you plan to work on moving forward?
References and Acknowledgements
If not already cited in other sections of your poster, what literature sources did you reference on this poster? Who helped or advised you?