Difference between revisions of "Team:CSU CHINA/Poster"
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| − | <p><span style="font-weight: | + | <p><span style="font-weight: bolder">Cadmium contamination</span> of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use <span style="font-weight: bolder">Synechocystis sp. PCC6803</span> with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.</p> |
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| − | <p>First of all, we <span style="font-weight: | + | <p>First of all, we <span style="font-weight: bolder">translate</span> cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by <span style="font-weight: bolder">chelating proteins</span>. At the same time, we increase the expression of <span style="font-weight: bolder">oxidoreductase</span> to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)</p> |
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<img src="https://static.igem.org/mediawiki/2020/3/3e/T--CSU_CHINA--Figure1-design.jpeg" /> | <img src="https://static.igem.org/mediawiki/2020/3/3e/T--CSU_CHINA--Figure1-design.jpeg" /> | ||
Revision as of 16:29, 10 November 2020
Poster: CSU_CHINA
Poster Template
This poster template will let you create an interactive poster! The poster is divided in two parts: the visual overview on the left and the documentation on the right. The visual overview is broken down into sections that the user can click on. When a user clicks on a poster section in the visual overview, the documentation on the right will display the text and graphics associated with this section of the poster. You can find the documentation on how to use this template, as well as an example here: https://2020.igem.org/Competition/Deliverables/Poster
Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.
First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)
Cadmium contamination of water worldwide is already having a severe environmental impact, especially on crops, such as cadmium rice. In addition, cadmium contamination is likely to cause itai-itai disease and renal tubular injury, after prolonged and/or high exposure may eventually to renal failure, thus, the problem of cadmium pollution of water needs to be solved urgently. We adopt an environmentally friendly biological approach——use Synechocystis sp. PCC6803 with a clear genetic background and a certain degree of tolerance to cadmium as a chassis organism for transformation.
First of all, we translate cadmium, which is enriched on the cell wall through transporter proteins. However, this may cause photosynthesis failure, protein inactivation or oxidative stress in Synechocystis and lead to cell death, making it difficult to achieve high tolerance rate. Therefore, after transporting large amounts of cadmium ions into the cells, we accumulate heavy metal ions and prevent them from being transported out of the cells by chelating proteins. At the same time, we increase the expression of oxidoreductase to remove reactive oxygen clusters in the cells, thus increasing the growth rate of algae under cadmium ion stress. Besides, for high expression of functional proteins, We optimized the codon preferences of the algae, and analyzed the protein expression results.(Figure 1)
