Template:Virginia/scripts/seqDataJS
/*This file is used to store the text from the .gb files in a form that's readable by other scripts*/ var sequences = {}; sequences.seq_2x_Res_6x_CoA = {}; sequences.seq_2x_Res_6x_CoA.varName = "seq_2x_Res_6x_CoA"; sequences.seq_2x_Res_6x_CoA.name = "(2x Res 6x CoA)"; sequences.seq_2x_Res_6x_CoA.seqType = "both"; sequences.seq_2x_Res_6x_CoA.data = `LOCUS (2x_Res,_6x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_binding 2668..2690
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3089..3097
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3078..3086
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3034..3042
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2923..2930
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_binding 2967..2989
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 2501..2625
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2702..2710)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3100..3108
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2744..2853
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3410..3418)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 2931..2965
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(3421..3429)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
HIV-RT Binding Site join(3518..3521,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3465..3473)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(3443..3451)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3509..3517)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3122..3130
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3377..3385)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
gBlock 3187..3311
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3023..3031
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3045..3053
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3487..3495)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 3310..3317
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2691..2699)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 3001..3009
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3498..3506)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3012..3020
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(3454..3462)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 3318..3352
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Terminator 3241..3309
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(3399..3407)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Terminator 2555..2623
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(3432..3440)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(3388..3396)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3056..3064
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3067..3075
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Scar 2624..2631
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 2854..2922
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3111..3119
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2990..2998
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Promoter 2632..2666
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2724..2732)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2735..2743)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
HIV-RT Binding Site 2445..2554
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
HIV-RT Binding Site 3131..3240
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3476..3484)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 3354..3376
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2713..2721)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
gBlock 2800..2924
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAAAAA ACGTGGCGCC
2761 CGAACAGGGA CGGATAGCTC AGTCGGTAGA GCATCAGACT TTTAATCTGA GGGTCCAGGG
2821 TTCAAGTCCC TGTTCGGGCG CCACGTTTTT TTTattagca gaaagtcaaa agcctccgac
2881 cggaggcttt tgactaaaac ttcccttggg gttatcattg ggtactagag TTGACGGCTA
2941 GCTCAGTCCT AGGTACAGTG CTAGCTGGAA TTGTGAGCGG ATAACAATTG AGGACGGCAT
3001 GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC
3061 TGGGATGTCG ATGCCATGCG GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
3121 TGCGGCTGGG AAAAAAAACG TGGCGCCCGA ACAGGGACGG ATAGCTCAGT CGGTAGAGCA
3181 TCAGACTTTT AATCTGAGGG TCCAGGGTTC AAGTCCCTGT TCGGGCGCCA CGTTTTTTTT
3241 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt
3301 atcattgggt actagagTTG ACGGCTAGCT CAGTCCTAGG TACAGTGCTA GCTGGAATTG
3361 TGAGCGGATA ACAATTCCCA GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT
3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
//`
sequences.seq_4x_Res_4x_CoA = {}; sequences.seq_4x_Res_4x_CoA.varName = "seq_4x_Res_4x_CoA"; sequences.seq_4x_Res_4x_CoA.name = "(4x Res 4x CoA)"; sequences.seq_4x_Res_4x_CoA.seqType = "both"; sequences.seq_4x_Res_4x_CoA.data = `LOCUS (4x_Res,_4x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site 3089..3097
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2779..2787)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3421..3429)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3122..3130
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 2599..2667
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 2447..2455
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3133..3141
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Terminator 2942..3010
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 3155..3163
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3454..3462)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2757..2765)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3465..3473)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 3055..3077
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_binding 2712..2734
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2668..2675
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 3111..3119
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3498..3506)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
HIV-RT Binding Site join(3518..3521,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2469..2477
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Scar 3354..3361
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site complement(2768..2776)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Promoter 3362..3396
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2735..2743)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 3144..3152
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2812..2820)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Promoter 3019..3053
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(3509..3517)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
HIV-RT Binding Site 3175..3284
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2790..2798)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3078..3086
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Terminator 3285..3353
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(3432..3440)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 3398..3420
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Scar 3011..3018
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
gBlock 3231..3355
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3443..3451)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site complement(2823..2831)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site complement(3476..3484)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
gBlock 2545..2669
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2480..2488
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2801..2809)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Promoter 2676..2710
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(3487..3495)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3100..3108
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2489..2598
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
gBlock 2888..3012
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2832..2941
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2746..2754)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2458..2466
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 3166..3174
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
2821 ATGCCGTCCT CAAAAAAAAC GTGGCGCCCG AACAGGGACG GATAGCTCAG TCGGTAGAGC
2881 ATCAGACTTT TAATCTGAGG GTCCAGGGTT CAAGTCCCTG TTCGGGCGCC ACGTTTTTTT
2941 Tattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt
3001 tatcattggg tactagagTT GACGGCTAGC TCAGTCCTAG GTACAGTGCT AGCTGGAATT
3061 GTGAGCGGAT AACAATTGAG GACGGCATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
3121 TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGAAAAAA
3181 AACGTGGCGC CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG
3241 AGGGTCCAGG GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa
3301 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga
3361 gTTGACGGCT AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT
3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
//`
sequences.seq_CoA_4x_Assembly = {}; sequences.seq_CoA_4x_Assembly.varName = "seq_CoA_4x_Assembly"; sequences.seq_CoA_4x_Assembly.name = "CoA 4x Assembly"; sequences.seq_CoA_4x_Assembly.seqType = "both"; sequences.seq_CoA_4x_Assembly.data = `LOCUS CoA_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AA
//`
sequences.seq_CoA_4x_Comp_Assembly = {}; sequences.seq_CoA_4x_Comp_Assembly.varName = "seq_CoA_4x_Comp_Assembly"; sequences.seq_CoA_4x_Comp_Assembly.name = "CoA 4x Comp Assembly"; sequences.seq_CoA_4x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_4x_Comp_Assembly.data = `LOCUS CoA_4x_Comp_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2425..2433)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2480..2488)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2469..2477)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2447..2455)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2414..2422)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2403..2411)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2436..2444)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2392..2400)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2458..2466)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
2461 AGCCGCATGG CATCGACATG CCGTCCTCAA AA
//`
sequences.seq_CoA_4x_Full_Part = {}; sequences.seq_CoA_4x_Full_Part.varName = "seq_CoA_4x_Full_Part"; sequences.seq_CoA_4x_Full_Part.name = "CoA 4x Full Part"; sequences.seq_CoA_4x_Full_Part.seqType = "both"; sequences.seq_CoA_4x_Full_Part.data = `LOCUS CoA_4x_Full_Part 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2779..2787)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
HIV-RT Binding Site 2489..2598
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2746..2754)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Scar 2668..2675
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site join(2832..2835,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2735..2743)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2768..2776)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2790..2798)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2801..2809)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Promoter 2676..2710
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2812..2820)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gBlock 2545..2669
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2757..2765)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 2599..2667
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 2712..2734
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2823..2831)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTCCCAGC CGCATGGCAT CGACATCCCA
2761 GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
2821 ATGCCGTCCT CAAAA
//`
sequences.seq_CoA_4x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.varName = "seq_CoA_4x_r_oligo_Complement_Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.name = "CoA 4x r_oligo Complement Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.data = `LOCUS CoA_4x_r_oligo_Complemen 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(42..50)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(53..61)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(64..72)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(75..83)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(86..94)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(97..105)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(108..116)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(119..127)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(130..138)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(42..138)
/label="Temp2"
/note="sequence: GAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 42..138
/label="Temp"
/note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTC"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
121 CATCGACATG CCGTCCTCAA AACGAGACCA ACCTG
//`
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.varName = "seq_CoA_4x_r_oligo_Duplex_126145_REV_1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.name = "CoA 4x r_oligo Duplex 126-145 REV 1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.data = `LOCUS CoA_4x_r_oligo_Duplex_126 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGTTTTCCC AGCCGCATGG
//`
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.varName = "seq_CoA_4x_r_oligo_Duplex_6988_FWD_1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.name = "CoA 4x r_oligo Duplex 69-88 FWD 1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.data = `LOCUS CoA_4x_r_oligo_Duplex_69- 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TGGGATGTCG ATGCCATGCG
//`
sequences.seq_CoA_4x_r_oligo_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Duplex.varName = "seq_CoA_4x_r_oligo_Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.name = "CoA 4x r_oligo Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex.data = `LOCUS CoA_4x_r_oligo_Duplex 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 53..61
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 64..72
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 75..83
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 86..94
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 97..105
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 108..116
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 119..127
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 130..138
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(126..145)
/label="CoA 4x r_oligo Duplex 126-145 REV 1"
/note="sequence: TCGTTTTCCCAGCCGCATGG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 18..138
/label="Temp Fwd"
/note="sequence: TGGAATTGTGAGCGGATAACAATTGAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(20..140)
/label="Temp Rev"
/note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTCAATTGTTATCCGCTCACAATTCCA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 69..88
/label="CoA 4x r_oligo Duplex 69-88 FWD 1"
/note="sequence: TGGGATGTCGATGCCATGCG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
121 CGATGCCATG CGGCTGGGAA AACGAGACCA ACCTG
//`
sequences.seq_CoA_6x_Assembly = {}; sequences.seq_CoA_6x_Assembly.varName = "seq_CoA_6x_Assembly"; sequences.seq_CoA_6x_Assembly.name = "CoA 6x Assembly"; sequences.seq_CoA_6x_Assembly.seqType = "both"; sequences.seq_CoA_6x_Assembly.data = `LOCUS CoA_6x_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2502..2510
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2491..2499
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2524..2532
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2513..2521
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
HIV-RT Binding Site join(2533..2536,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
2521 CATGCGGCTG GGAAAA
//`
sequences.seq_CoA_6x_Comp_Assembly = {}; sequences.seq_CoA_6x_Comp_Assembly.varName = "seq_CoA_6x_Comp_Assembly"; sequences.seq_CoA_6x_Comp_Assembly.name = "CoA 6x Comp Assembly"; sequences.seq_CoA_6x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_6x_Comp_Assembly.data = `LOCUS CoA_6x_Comp_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site complement(2491..2499)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2502..2510)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2480..2488)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2458..2466)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2447..2455)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site complement(2436..2444)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2425..2433)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2414..2422)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2403..2411)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2392..2400)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2513..2521)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2524..2532)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
HIV-RT Binding Site join(2533..2536,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2469..2477)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
2461 AGCCGCATGG CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA
2521 CATGCCGTCC TCAAAA
//`
sequences.seq_CoA_6x_Full_Part = {}; sequences.seq_CoA_6x_Full_Part.varName = "seq_CoA_6x_Full_Part"; sequences.seq_CoA_6x_Full_Part.name = "CoA 6x Full Part"; sequences.seq_CoA_6x_Full_Part.seqType = "both"; sequences.seq_CoA_6x_Full_Part.data = `LOCUS CoA_6x_Full_Part 2923 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2756..2778
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2790..2798)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2900..2908)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2867..2875)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2533..2642
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2524..2532
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site complement(2823..2831)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2812..2820)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2878..2886)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2491..2499
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2856..2864)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2502..2510
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Terminator 2643..2711
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2834..2842)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2513..2521
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2779..2787)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 2720..2754
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2845..2853)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2712..2719
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 2589..2713
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2889..2897)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2801..2809)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site join(2920..2923,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2911..2919)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
2521 CATGCGGCTG GGAAAAAAAA CGTGGCGCCC GAACAGGGAC GGATAGCTCA GTCGGTAGAG
2581 CATCAGACTT TTAATCTGAG GGTCCAGGGT TCAAGTCCCT GTTCGGGCGC CACGTTTTTT
2641 TTattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact tcccttgggg
2701 ttatcattgg gtactagagT TGACGGCTAG CTCAGTCCTA GGTACAGTGC TAGCTGGAAT
2761 TGTGAGCGGA TAACAATTCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
2821 ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT CGACATCCCA GCCGCATGGC
2881 ATCGACATCC CAGCCGCATG GCATCGACAT GCCGTCCTCA AAA
//`
sequences.seq_CoA_6x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.varName = "seq_CoA_6x_r_oligo_Complement_Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.name = "CoA 6x r_oligo Complement Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.data = `LOCUS CoA_6x_r_oligo_Complemen 199 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(42..50)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(53..61)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(64..72)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(75..83)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(86..94)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(97..105)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(108..116)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(119..127)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(130..138)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(141..149)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(152..160)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(163..171)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(174..182)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
121 CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA CATGCCGTCC
181 TCAAAACGAG ACCAACCTG
//`
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.varName = "seq_CoA_6x_r_oligo_Duplex_113132_FWD_1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.name = "CoA 6x r_oligo Duplex 113-132 FWD 1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.data = `LOCUS CoA_6x_r_oligo_Duplex_113 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TGGGATGTCG ATGCCATGCG
//`
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.varName = "seq_CoA_6x_r_oligo_Duplex_170189_REV_1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.name = "CoA 6x r_oligo Duplex 170-189 REV 1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.data = `LOCUS CoA_6x_r_oligo_Duplex_170 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGTTTTCCC AGCCGCATGG
//`
sequences.seq_CoA_6x_r_oligo_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Duplex.varName = "seq_CoA_6x_r_oligo_Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.name = "CoA 6x r_oligo Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex.data = `LOCUS CoA_6x_r_oligo_Duplex 199 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 53..61
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 64..72
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 75..83
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 86..94
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 97..105
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 108..116
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 119..127
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 130..138
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 141..149
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 152..160
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 163..171
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 174..182
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 113..132
/label="CoA 6x r_oligo Duplex 113-132 FWD 1"
/note="sequence: TGGGATGTCGATGCCATGCG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(170..189)
/label="CoA 6x r_oligo Duplex 170-189 REV 1"
/note="sequence: TCGTTTTCCCAGCCGCATGG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
121 CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG
181 GGAAAACGAG ACCAACCTG
//`
sequences.seq_gBlock_A = {}; sequences.seq_gBlock_A.varName = "seq_gBlock_A"; sequences.seq_gBlock_A.name = "gBlock A"; sequences.seq_gBlock_A.seqType = "linear"; sequences.seq_gBlock_A.data = `LOCUS gBlock_A 995 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 863..870
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 785..790
/label="double stop codon"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 18..979
/label="gBlock A"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
stem_loop 883..905
/label="stem loop"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature 110..815
/label="RFP"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 82..89
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 97..101
/label="strong RBS"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 110..815
/label="BBa_E1010 (RFP)"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 110..112
/label="start codon"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 90..104
/label="BBa_B0030 (strong RBS)"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 822..862
/label="BBa_B0012"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 822..916
/label="BBa_B0014 (double terminator)"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 871..916
/label="BBa_B0011"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 18..39
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 90..916
/label="From BBa_K3114015"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 794..799
/label="double stop codon"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
misc_feature 40..74
/label="BBa_J23105"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 GACTTACGTC TCGtctggaa ttcgcggccg cttctagagt ttacggctag ctcagtccta
61 ggtactatgc tagcCGAGAC Ctactagaga ttaaagagga gaaatactaa tggcttcctc
121 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
181 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
241 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
301 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
361 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
421 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
481 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
541 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
601 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
661 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
721 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
781 tgcttaataa cgctgatagt gctagtgtag atcgctaata atcacactgg ctcaccttcg
841 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
901 ggctttttta ttatttGGTC TCGAAAAAAA ACGTGGCGCC CGAACAGGGA CGGATAGCTC
961 AGTCGGTAGA GCATCAGACT TTCGAGACGC TTGTG
//`
sequences.seq_gBlock_B = {}; sequences.seq_gBlock_B.varName = "seq_gBlock_B"; sequences.seq_gBlock_B.name = "gBlock B"; sequences.seq_gBlock_B.seqType = "linear"; sequences.seq_gBlock_B.data = `LOCUS gBlock_B 175 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 138..158
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 15..158
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc 72..111
/label="identical to E. coli MG1655 between tonB and yciA"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 69..137
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
stem_loop 79..110
/label="stem loop"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 GATCGTCGTC TCCCTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC GGGCGCCACG
61 TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc
121 ttggggttat cattgggtac tagtagcggc cgctgcagag tcCGAGACGT TCCAT
//`
sequences.seq_GGE114 = {}; sequences.seq_GGE114.varName = "seq_GGE114"; sequences.seq_GGE114.name = "GGE114"; sequences.seq_GGE114.seqType = "both"; sequences.seq_GGE114.data = `LOCUS (Addgene_#120731_)_GGE1 5268 bp ds-DNA circular 06-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
terminator 4070..4113
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2002..4115
/label="pSB1A3 Region"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
protein_bind 4452..4485
/label="loxP"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
source 1..5268
/label="source"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 3638..3657
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter complement(3876..3980)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS 722..1399
/label="mRFP1"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS complement(3015..3875)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
rep_origin complement(2256..2844)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
promoter 632..662
/label="lac promoter"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(2085..2102)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
terminator 2004..2061
/label="his operon terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
protein_bind 670..686
/label="lac operator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
protein_bind 596..617
/label="CAP binding site"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
terminator 1444..1515
/label="rrnB T1 terminator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind 4048..4066
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(2336..2355)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 3156..3156
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 1531..1558
/label="T7Te terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
RBS 704..715
/label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer 2002..2019
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(4088..4115)
/label="BBa_Prefix & Promoter SDM Rev"
/note="sequence: cagaaatcatccttagcgaaagctaagg"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(4093..4115)
/label="BsaI pSB1A3 Rev"
/note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4116..4135
/label="BBa_Prefix & Promoter SDM Fwd"
/note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 2002..2019
/label="BsaI pSB1A3 Fwd"
/note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(4093..4115)
/label="Linear pSB1A3 BsmBI Rev"
/note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4093..4115)
/label="pSB1A3 SapI rev"
/note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 2002..2019
/label="pSB1A3 fwd SapI"
/note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 2002..2023
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
ORIGIN
1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
//`
sequences.seq_Linear_pSB1A3_BsmBI_Fwd = {}; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.varName = "seq_Linear_pSB1A3_BsmBI_Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.name = "Linear pSB1A3 BsmBI Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.data = `LOCUS Linear_pSB1A3_BsmBI_Fwd 31 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CTATTTCGTC TCCagtccgg caaaaaaggg c
//`
sequences.seq_Linear_pSB1A3_BsmBI_Rev = {}; sequences.seq_Linear_pSB1A3_BsmBI_Rev.varName = "seq_Linear_pSB1A3_BsmBI_Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.name = "Linear pSB1A3 BsmBI Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.data = `LOCUS Linear_pSB1A3_BsmBI_Rev 36 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATTCCCCGTC TCCcagaaat catccttagc gaaagc
//`
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites = {}; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.varName = "seq_Linear_pSB1A3_with_BsmBI_Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.name = "Linear pSB1A3 with BsmBI Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.seqType = "linear"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.data = `LOCUS Linearized_pSB1A3_w/_Bs 2140 bp ds-DNA linear 24-JUN-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
CDS complement(1027..1887)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1650..1669
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind complement(97..114)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer_bind 2060..2078
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(348..367)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 14..2127
/label="pSB1A3 Region"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1168..1168
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2082..2125
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
terminator 16..73
/label="his operon terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
promoter complement(1888..1992)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
rep_origin complement(268..856)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(2100..2127)
/label="BBa_Prefix & Promoter SDM Rev"
/note="sequence: cagaaatcatccttagcgaaagctaagg"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 14..35
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
ORIGIN
1 CTATTTCGTC TCCagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
241 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
1141 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc
1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
1741 gagatccagt tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt
1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
1981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
2041 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
2101 cttagctttc gctaaggatg atttctgGGA GACGGGGAAT
//`
sequences.seq_Resveratrol_2x_Assembly = {}; sequences.seq_Resveratrol_2x_Assembly.varName = "seq_Resveratrol_2x_Assembly"; sequences.seq_Resveratrol_2x_Assembly.name = "Resveratrol 2x Assembly"; sequences.seq_Resveratrol_2x_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Assembly.data = `LOCUS Resveratrol_2x_Assembly 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
HIV-RT Binding Site join(2445..2448,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAA
//`
sequences.seq_Resveratrol_2x_Comp_Assembly = {}; sequences.seq_Resveratrol_2x_Comp_Assembly.varName = "seq_Resveratrol_2x_Comp_Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.name = "Resveratrol 2x Comp Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Comp_Assembly.data = `LOCUS Resveratrol_2x_Comp_Ass 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
HIV-RT Binding Site join(2445..2448,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2436..2444)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2425..2433)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2414..2422)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site complement(2403..2411)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2392..2400)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAA
//`
sequences.seq_Resveratrol_2x_Full_Part = {}; sequences.seq_Resveratrol_2x_Full_Part.varName = "seq_Resveratrol_2x_Full_Part"; sequences.seq_Resveratrol_2x_Full_Part.name = "Resveratrol 2x Full Part"; sequences.seq_Resveratrol_2x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_2x_Full_Part.data = `LOCUS Resveratrol_2x_Full_Par 2747 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Promoter 2632..2666
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2445..2554
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
HIV-RT Binding Site join(2744..2747,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2735..2743)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 2668..2690
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2713..2721)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Scar 2624..2631
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Terminator 2555..2623
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site complement(2724..2732)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2702..2710)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2691..2699)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gBlock 2501..2625
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAA
//`
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.name = "Resveratrol 2x r_oligo Complement Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_C 111 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature complement(42..50)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(53..61)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(64..72)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(75..83)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(86..94)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
61 CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAACG AGACCAACCT G
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.name = "Resveratrol 2x r_oligo Duplex 11-33 FWD 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 23 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCCTAGCTGG AATTGTGAGC GGA
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.name = "Resveratrol 2x r_oligo Duplex 75-94 REV 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTTCCACACA TCGCCCACGC
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.name = "Resveratrol 2x r_oligo Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_D 111 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 53..61
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 64..72
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 75..83
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 86..94
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(75..94)
/label="Resveratrol 2x r_oligo Duplex 75-94 REV 1"
/note="sequence: TTTCCACACATCGCCCACGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 11..33
/label="Resveratrol 2x r_oligo Duplex 11-33 FWD 1"
/note="sequence: TCCTAGCTGGAATTGTGAGCGGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAACG AGACCAACCT G
//`
sequences.seq_Resveratrol_4x_Assembly = {}; sequences.seq_Resveratrol_4x_Assembly.varName = "seq_Resveratrol_4x_Assembly"; sequences.seq_Resveratrol_4x_Assembly.name = "Resveratrol 4x Assembly"; sequences.seq_Resveratrol_4x_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Assembly.data = `LOCUS Resveratrol_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2447..2455
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2469..2477
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2458..2466
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2480..2488
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AA
//`
sequences.seq_Resveratrol_4x_Comp_Assembly = {}; sequences.seq_Resveratrol_4x_Comp_Assembly.varName = "seq_Resveratrol_4x_Comp_Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.name = "Resveratrol 4x Comp Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Comp_Assembly.data = `LOCUS Resveratrol_4x_Comp_Ass 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2469..2477)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2392..2400)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(2458..2466)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2480..2488)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2414..2422)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(2436..2444)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2425..2433)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2403..2411)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2447..2455)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT
2461 CCACACATCG CCCACGCATG CCGTCCTCAA AA
//`
sequences.seq_Resveratrol_4x_Full_Part = {}; sequences.seq_Resveratrol_4x_Full_Part.varName = "seq_Resveratrol_4x_Full_Part"; sequences.seq_Resveratrol_4x_Full_Part.name = "Resveratrol 4x Full Part"; sequences.seq_Resveratrol_4x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_4x_Full_Part.data = `LOCUS Resveratrol_4x_Full_Par 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site complement(2823..2831)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site complement(2768..2776)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2480..2488
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Scar 2668..2675
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2812..2820)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2779..2787)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
HIV-RT Binding Site 2489..2598
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2458..2466
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
HIV-RT Binding Site join(2832..2835,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2735..2743)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_binding 2712..2734
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 2599..2667
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2469..2477
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 2676..2710
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2790..2798)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gBlock 2545..2669
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2757..2765)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 2447..2455
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2801..2809)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2746..2754)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
2821 ATGCCGTCCT CAAAA
//`
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.name = "Resveratrol 4x r_oligo Complement Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_C 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature complement(42..50)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(53..61)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(64..72)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(75..83)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(86..94)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(97..105)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(108..116)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(119..127)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(130..138)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
61 CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT CCACACATCG
121 CCCACGCATG CCGTCCTCAA AACGAGACCA ACCTG
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.name = "Resveratrol 4x r_oligo Duplex 109-128 REV 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGCCCACGC ATTTTCCACA
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.name = "Resveratrol 4x r_oligo Duplex 39-58 FWD 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATTGAGGACG GCATGCGTGG
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.name = "Resveratrol 4x r_oligo Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_D 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 53..61
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 64..72
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 75..83
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 86..94
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 97..105
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 108..116
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 119..127
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 130..138
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 39..58
/label="Resveratrol 4x r_oligo Duplex 39-58 FWD 1"
/note="sequence: ATTGAGGACGGCATGCGTGG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(109..128)
/label="Resveratrol 4x r_oligo Duplex 109-128 REV 1"
/note="sequence: TCGCCCACGCATTTTCCACA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG TGGAAAATGC
121 GTGGGCGATG TGTGGAAAAA AACGAGACCA ACCTG
//`