/*This file is used to store the text from the .gb files in a form that's readable by other scripts*/ var sequences = {}; sequences.seq_2x_Res_6x_CoA = {}; sequences.seq_2x_Res_6x_CoA.varName = "seq_2x_Res_6x_CoA"; sequences.seq_2x_Res_6x_CoA.name = "(2x Res 6x CoA)"; sequences.seq_2x_Res_6x_CoA.seqType = "both"; sequences.seq_2x_Res_6x_CoA.data = `LOCUS (2x_Res,_6x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_binding 2668..2690 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 3089..3097 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 3078..3086 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 3034..3042 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Scar 2923..2930 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_binding 2967..2989 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc gBlock 2501..2625 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2702..2710) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 3100..3108 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site 2744..2853 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(3410..3418) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Promoter 2931..2965 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(3421..3429) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac HIV-RT Binding Site join(3518..3521,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(3465..3473) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(3443..3451) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2436..2444 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site complement(3509..3517) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 3122..3130 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(3377..3385) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 gBlock 3187..3311 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 3023..3031 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 3045..3053 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(3487..3495) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Scar 3310..3317 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Zinc Finger Site complement(2691..2699) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site 2425..2433 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2414..2422 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site 3001..3009 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(3498..3506) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 3012..3020 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(3454..3462) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Promoter 3318..3352 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Terminator 3241..3309 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(3399..3407) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Terminator 2555..2623 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(3432..3440) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(3388..3396) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 3056..3064 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 3067..3075 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Scar 2624..2631 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Terminator 2854..2922 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 3111..3119 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2990..2998 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Promoter 2632..2666 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2724..2732) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2735..2743) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 HIV-RT Binding Site 2445..2554 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 HIV-RT Binding Site 3131..3240 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(3476..3484) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2403..2411 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_binding 3354..3376 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2713..2721) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 gBlock 2800..2924 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC 2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG 2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga 2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT 2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA 2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAAAAA ACGTGGCGCC 2761 CGAACAGGGA CGGATAGCTC AGTCGGTAGA GCATCAGACT TTTAATCTGA GGGTCCAGGG 2821 TTCAAGTCCC TGTTCGGGCG CCACGTTTTT TTTattagca gaaagtcaaa agcctccgac 2881 cggaggcttt tgactaaaac ttcccttggg gttatcattg ggtactagag TTGACGGCTA 2941 GCTCAGTCCT AGGTACAGTG CTAGCTGGAA TTGTGAGCGG ATAACAATTG AGGACGGCAT 3001 GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC 3061 TGGGATGTCG ATGCCATGCG GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA 3121 TGCGGCTGGG AAAAAAAACG TGGCGCCCGA ACAGGGACGG ATAGCTCAGT CGGTAGAGCA 3181 TCAGACTTTT AATCTGAGGG TCCAGGGTTC AAGTCCCTGT TCGGGCGCCA CGTTTTTTTT 3241 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt 3301 atcattgggt actagagTTG ACGGCTAGCT CAGTCCTAGG TACAGTGCTA GCTGGAATTG 3361 TGAGCGGATA ACAATTCCCA GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT 3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT 3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
//`
sequences.seq_4x_Res_4x_CoA = {}; sequences.seq_4x_Res_4x_CoA.varName = "seq_4x_Res_4x_CoA"; sequences.seq_4x_Res_4x_CoA.name = "(4x Res 4x CoA)"; sequences.seq_4x_Res_4x_CoA.seqType = "both"; sequences.seq_4x_Res_4x_CoA.data = `LOCUS (4x_Res,_4x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site 3089..3097 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2779..2787) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site complement(3421..3429) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 3122..3130 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 2599..2667 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site 2447..2455 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 3133..3141 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Terminator 2942..3010 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 3155..3163 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(3454..3462) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2757..2765) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site complement(3465..3473) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_binding 3055..3077 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_binding 2712..2734 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Scar 2668..2675 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 3111..3119 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(3498..3506) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e HIV-RT Binding Site join(3518..3521,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2469..2477 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Scar 3354..3361 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site complement(2768..2776) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Promoter 3362..3396 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2735..2743) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2414..2422 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site 3144..3152 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2812..2820) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Promoter 3019..3053 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(3509..3517) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 HIV-RT Binding Site 3175..3284 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2790..2798) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 3078..3086 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Terminator 3285..3353 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(3432..3440) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2403..2411 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_binding 3398..3420 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Scar 3011..3018 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 gBlock 3231..3355 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2436..2444 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site complement(3443..3451) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site complement(2823..2831) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site complement(3476..3484) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e gBlock 2545..2669 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2480..2488 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Zinc Finger Site complement(2801..2809) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Promoter 2676..2710 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(3487..3495) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 3100..3108 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site 2489..2598 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 gBlock 2888..3012 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site 2832..2941 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2746..2754) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2425..2433 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2458..2466 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site 3166..3174 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG 2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT 2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC 2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact 2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA 2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC 2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC 2821 ATGCCGTCCT CAAAAAAAAC GTGGCGCCCG AACAGGGACG GATAGCTCAG TCGGTAGAGC 2881 ATCAGACTTT TAATCTGAGG GTCCAGGGTT CAAGTCCCTG TTCGGGCGCC ACGTTTTTTT 2941 Tattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt 3001 tatcattggg tactagagTT GACGGCTAGC TCAGTCCTAG GTACAGTGCT AGCTGGAATT 3061 GTGAGCGGAT AACAATTGAG GACGGCATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA 3121 TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGAAAAAA 3181 AACGTGGCGC CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG 3241 AGGGTCCAGG GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa 3301 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga 3361 gTTGACGGCT AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT 3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT 3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
//`
sequences.seq_Addgene_109383_pSB1C3Lux = {}; sequences.seq_Addgene_109383_pSB1C3Lux.varName = "seq_Addgene_109383_pSB1C3Lux"; sequences.seq_Addgene_109383_pSB1C3Lux.name = "(Addgene 109383) pSB1C3-Lux"; sequences.seq_Addgene_109383_pSB1C3Lux.seqType = "both"; sequences.seq_Addgene_109383_pSB1C3Lux.data = `LOCUS (Addgene_#109383)_pSB1C 9707 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_109383_206254" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
terminator 1..58 /label="his operon terminator" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 rep_origin complement(253..841) /label="ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 1024..1118 /label="lambda t0 terminator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 promoter 2955..3239 /label="araBAD promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 terminator 1982..2025 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2028..2049 /label="BioBrick prefix" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 CDS complement(1139..1798) /label="CmR" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer_bind complement(82..99) /label="L4440" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature join(9687..9707,1..2049) /label="pSB1C3 Backbone Region" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 source 1..9707 /label="source" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind 1960..1978 /label="pBRforEco" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 CDS complement(2050..2928) /label="araC" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind complement(333..352) /label="pBR322ori-F" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind 3162..3181 /label="pBAD Forward" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 9687..9707 /label="BioBrick suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 9647..9650 /label="Overhang for end of pduD part" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind 1713..1732 /label="CAT-R" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer complement(2062..2093) /label="pSB1C3 Reverse Primer" /note="sequence: ACTGATCGTCTCTgttgtgaagaaaaagtgaatgatgtagccgtc" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer 9647..9670 /label="pSB1C3 Forward Primer" /note="sequence: TATGATCGTCTCAgtcagaacagatgtttgccgatgc" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2
ORIGIN
1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt 61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc 301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct 1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt 1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa 1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta 1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac 1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg 2041 cttctagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg 2101 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc 2161 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag 2221 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg 2281 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat 2341 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt 2401 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc 2461 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat 2521 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt 2581 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta 2641 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc 2701 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac 2761 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc 2821 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc 2881 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact 2941 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt 3001 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta 3061 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag 3121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc 3181 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata 3241 cccgtttttt tgggctagca gggaaaataa tgaataaatg tattccaatg ataattaatg 3301 gaatgattca agattttgat aattatgcat ataaagaagt taaactaaat aatgataata 3361 gagtaaaatt atctgtcatt actgaaagtt cagtttcaaa aacattaaat atcaaagata 3421 gaattaatct aaatttaaat cagattgtga attttttata taccgttggt caacgatgga 3481 aaagtgaaga atataatcgg cgacgaacct atatccgtga gttaaaaaca tatcttggtt 3541 attctgatga aatggcaaga ttagaagcga attggattgc aatgttattg tgctctaaaa 3601 gtgctttgta tgacattgtt aattatgatt tgggctctat acacgtatta gatgaatggc 3661 ttccacgtgg tgattgctat gttaaagcac aaccgaaagg tgtttctgtt cacttgttag 3721 ctggtaatgt tccattatca ggagtgacat ctattttgcg tgctatttta acaaaaaatg 3781 agtgcattat taaaacttcg tcttcagatc cttttactgc aaacgcttta gtttccagtt 3841 ttattgatgt taatgcagac catccaataa ccaaatcaat gtctgttatg tattggccgc 3901 atgatgaaga tatgactcta tctcaaagaa taatgaatca tgccgacgtg gttattgctt 3961 ggggtggaga cgaggcgatt aaatgggcgg taaaatattc accaccgcat gtcgatattc 4021 tgaaatttgg accaaagaaa agcttaagta ttattgaagc tcctaaagat atagaagccg 4081 cagcaatggg ggttgctcat gatatttgtt tctatgacca gcaagcctgc ttctctactc 4141 aagacgttta ttatatagga gataatttac ctttattttt aaatgaactt gaaaaacagc 4201 tagatcgata cgcgaaaatt ttaccaaaag gttcaaatag ttttgatgaa aaagcggcgt 4261 ttactcttac tgaaaaagaa agtctatttg ctggatatga agtgagaaag ggagataagc 4321 aagcttggtt aatagtcgta tcacctacaa atagctttgg aaatcaaccg ctatcacgaa 4381 gtgtgtatgt tcatcaagta tctgatatta aagagataat tccttttgtt aataaaaata 4441 gaacacaaac tgtttctatt tatccttggg aagcgtcatt aaaatatcga gataaattag 4501 caagaagtgg agttgaaaga attgttgaat caggcatgaa taatattttc agagttggag 4561 gggctcatga ttcattatct cctctccagt acctagttag gtttgtatcg catgagagac 4621 catttaatta tacgacaaaa gatgttgcgg ttgaaatcga acaaacacgt tacttagagg 4681 aagataagtt tttagttttt gtcccatagt taaaggaaat tatatgaaag atgaaagtgc 4741 tttttttacg attgatcaca tcatcaagct tgataatggt cagtctatcc gagtttggga 4801 aacactccct aaaaagaacg taccagagaa aaaacataca atacttattg cttcgggttt 4861 tgctagaaga atggatcatt ttgcaggtct tgctgagtat ttatctacta acggttttca 4921 tgtcattcgc tacgattctt tgcatcatgt tggattaagc agtggatgta taaatgaatt 4981 tacgatgtcg attggaaaaa atagcctgct tacagtcgta gattggctta cagatcatgg 5041 tgtcgaacga atagggctga ttgctgctag tttgtcagcg agaatcgcct atgaggtagt 5101 aaataaaatt aaattatcat ttttaattac ggccgtaggt gtcgttaatc ttagagatac 5161 attagaaaaa gcattggagt atgactattt gcaattacct atttcagagt taccagaaga 5221 tcttgacttt gaaggtcata atttaggatc ggaggtcttt gttacagatt gctttaaaca 5281 taattgggac acgttagact cgacacttaa tagtgttaaa ggattagcga ttccatttat 5341 tgcttttact gcaaacgatg atagttgggt aaagcaaagt gaagttatag agctcattga 5401 tagcattgaa tctagtaatt gtaagctcta ttcgctaatt ggaagttcac atgatcttgg 5461 ggaaaatttg gttgtattaa gaaattttta tcaatcagtg acgaaggcag ccttagcatt 5521 agatgatggt ttattggatt tagagataga cattattgaa cctcgatttg aggacgttac 5581 aagtattact gttaaggagc gtagattaaa aaatgaaatt gaaaatgaat tattagaatt 5641 ggcttaaata aacagaatca ccaaaaagga atagagtatg aagtttggaa atatttgttt 5701 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg 5761 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga 5821 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac 5881 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga 5941 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg 6001 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac 6061 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag 6121 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc 6181 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc 6241 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa 6301 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat 6361 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa 6421 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg 6481 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa 6541 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga 6601 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc 6661 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc 6721 tttcttaaaa gaacctaaat aaattactta tttgatacta gagataataa ggaacaagtt 6781 atgaaatttg gattattttt tctaaacttt cagaaagatg gaataacatc tgaagaaacg 6841 ttggataata tggtaaagac tgtcacgtta attgattcaa ctaaatatca ttttaatact 6901 gcctttgtta atgaacatca cttttcaaaa aatggtattg ttggagcacc tattaccgca 6961 gctggttttt tattagggtt aacaaataaa ttacatattg gttcattaaa tcaagtaatt 7021 accacccatc accctgtacg tgtagcagaa gaagccagtt tattagatca aatgtcagag 7081 ggacgcttca ttcttggttt tagtgactgc gaaagtgatt tcgaaatgga attttttaga 7141 cgtcatatct catcaaggca acaacaattt gaagcatgct atgaaataat taatgacgca 7201 ttaactacag gttattgtca tccccaaaac gacttttatg attttccaaa ggtttcaatt 7261 aatccacact gttacagtga gaatggacct aagcaatatg tatccgctac atcaaaagaa 7321 gtcgtcatgt gggcagcgaa aaaggcactg cctttaacat ttaagtggga ggataattta 7381 gaaaccaaag aacgctatgc aattctatat aataaaacag cacaacaata tggtattgat 7441 atttcggatg ttgatcatca attaactgta attgcgaact taaatgctga tagaagtacg 7501 gctcaagaag aagtgagaga atacttaaaa gactatatca ctgaaactta ccctcaaatg 7561 gacagagatg aaaaaattaa ctgcattatt gaagagaatg cagttgggtc tcatgatgac 7621 tattatgaat cgacaaaatt agcagtggaa aaaacagggt ctaaaaatat tttattatcc 7681 tttgaatcaa tgtccgatat taaagatgta aaagatatta ttgatatgtt gaaccaaaaa 7741 atcgaaatga atttaccata ataaaattaa aggcaatttc tatattagat tgccttttta 7801 aatttctgtt gatattaggt attactggag agggtatgac tgtccatact gaatataaaa 7861 gaaatcaaat cattgctagt tcagaaattg atgatcttat ctttatgacg aaaccacaag 7921 agtggtcatt tgaagagcaa aaagaaatac gggataaatt agttcgtgag gctttttatt 7981 ttcactacaa tagaaatgaa gaatatagaa attattgtat caatcagcat gtgagtgata 8041 atttacacac tattgatgaa atacccgtgt ttccaacatc tgtttttaaa tataagaaat 8101 tacatactgt cacagccgag gacattgaaa attggtatac aagtagtgga actcgtggag 8161 taaaaagtca tattgcacgt gatcgtctta gcattgaacg cttgcttggt tctgtcaact 8221 tcggaatgaa atacgttgga gattggtttg agcatcaaat ggaattgata aatttaggac 8281 cagatagatt caatacaaat aatatttggt ttaaatatgt catgagtttg gtcgagttac 8341 tttatccgac tgaatttaca gttgataatg acaaaataga ttttgaaaaa acagtaaaac 8401 atctatttag aattaagaat agtaaaaaag acatttgctt aattgggcca ccattttttg 8461 tgtatctttt gtgccaatat atgaaagaaa acaatattga atttaaagga ggagatagag 8521 tacatattat tactggtgga ggatggaaat ctaatcagaa tgactcttta gatcgtgctg 8581 attttaatca attattaatg gatactttcc aactcgacaa gattaatcaa attagagata 8641 cctttaatca agttgagctt aatacttgtt tttttgaaga tgaatttcaa agaaaacatg 8701 ttccaccgtg ggtatatgct cgggctcttg atcctgaaac cttgaaaccc gtagcagatg 8761 gtgagatcgg gttgttaagt tatatggacg cctcatcaac tgcttaccct gcttttattg 8821 ttactgatga tatcggtatt gtaaaagaaa ttagagaacc agatccttac ccaggggtaa 8881 ctgttgagat tgttcggcgc ttaaatacac gtgcgcaaaa aggatgcgcg ctctctatgg 8941 ctaatgtcat acaaaagaat atcaaggatt aagttatgat tgttgatggt agagtttcaa 9001 aaatagtatt agcgtcaata agaaataata tatataaggt atttattact gtaaattcac 9061 caataaagtt catcgctgga caatttgtaa tggtcacgat taatgggaaa aaatgccctt 9121 tttcaattgc gaattgcccg acaaaaaatt acgaaataga attgcatatt ggtagttcga 9181 atagagactg ctcattggat attatcgaat attttgtcga tgctcttgtt gaggaagtcg 9241 caattgagtt agatgctccc catggaaacg cttggttacg gtctgaaagt aataacccat 9301 tgctattaat tgcgggaggt acaggtttat catatataaa tagcattcta acaaattgct 9361 taaataggaa tatacctcaa gatatttatc tttactgggg agtaaaagac agttctcttt 9421 tgtatgaaga tgaagagtta ctaaacttat cactaaacaa caaaaacttt cattatattc 9481 ctgttattga agataaaagt gaagaatgga tagggagaaa aggcactgtt cttgatgctg 9541 tcatggaaga ttttactgat cttacttatt ttgatattta tgtttgtgga cccttcatga 9601 tggctaaaac agcaaaagaa aaattaattg aagagaaaaa agcaaagtca gaacagatgt 9661 ttgccgatgc ttttgcatac gtataatact agtagcggcc gctgcag
//`
sequences.seq_4CL_Intro_gblock = {}; sequences.seq_4CL_Intro_gblock.varName = "seq_4CL_Intro_gblock"; sequences.seq_4CL_Intro_gblock.name = "4CL Intro gblock"; sequences.seq_4CL_Intro_gblock.seqType = "linear"; sequences.seq_4CL_Intro_gblock.data = `LOCUS 4CL_Intro_gblock 480 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 437..466 /label="Spacer" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 40..73 /label="BBa_J23102" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 122..436 /label="ZIF268" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 98..111 /label="BBa_J61115" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Biobricking 18..39 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_binding 74..97 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 CGTCAGGCTC TTCACACgaa ttcgcggccg cttctagagt gacagctagc tcagtcctag 61 gtactgtgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGGGATA Ctaaatacat 121 gATGCACCAC CATCACCATC ATCCCGGTGA GAAACCATAT GCGTGCCCGG TGGAAAGCTG 181 TGATAGACGC TTTAGCCGTA GTGACGAACT GACCCGCCAC ATTCGAATAC ATACGGGGCA 241 AAAACCGTTT CAGTGCCGCA TTTGTATGCG TAACTTCTCA CGGAGTGATC ATTTAACCAC 301 TCATATTCGT ACTCATACAG GCGAAAAACC TTTTGCCTGC GACATCTGTG GTCGCAAATT 361 CGCACGTTCC GATGAGCGCA AGCGTCACAC GAAAATCCAT ACCGGTGGCG GGAGCGGCGG 421 CGGATCTGGT GGCTCGggtg gaagtggagg tggaagtgga ggtagtAGAA GAGCTGCAAG
//`
sequences.seq_4CL_Outro_gblock = {}; sequences.seq_4CL_Outro_gblock.varName = "seq_4CL_Outro_gblock"; sequences.seq_4CL_Outro_gblock.name = "4CL Outro gblock"; sequences.seq_4CL_Outro_gblock.seqType = "linear"; sequences.seq_4CL_Outro_gblock.data = `LOCUS 4CL_Outro_gblock 137 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
stem_loop 15..66 /label="T0 terminator" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Biobricking 67..87 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 TTCCGGGCTC TTCAttgttc agaacgctcg gtcttgcaca ccgggcgttt tttctttgtg 61 agtccatact agtagcggcc gctgcagGGA AGAAGAGCtc caagagagtg aagggatgtc 121 aagctgccat gAATTGA
//`
sequences.seq_ACC_intro_gblock = {}; sequences.seq_ACC_intro_gblock.varName = "seq_ACC_intro_gblock"; sequences.seq_ACC_intro_gblock.name = "ACC intro gblock"; sequences.seq_ACC_intro_gblock.seqType = "both"; sequences.seq_ACC_intro_gblock.data = `LOCUS ACC_intro_gblock 2034 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 99..112 /label="RBS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 40..74 /label="Promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 113..1885 /label="ACCalpha" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1889..2017 /label="Terminator" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_binding 75..98 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 18..39 /label="Prefix" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86
ORIGIN
1 AATGCTGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta 61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGAGGTGAC ATATGAGCGT 121 GGAAACCCGT AAAATCACTA AAGTTTTGGT CGCGAACCGG GGTGAAATCG CTATTCGCGT 181 TTTTCGTGCG GCCCGCGATG AGGGTATCGG CAGTGTTGCT GTCTACGCCG AGCCGGATGC 241 CGATGCGCCA TTCGTGAGCT ATGCGGACGA AGCCTTTGCC CTGGGCGGCC AGACATCGGC 301 CGAATCGTAT CTGGTAATCG ACAAAATCAT TGACGCCGCT CGTAAGAGCG GGGCTGATGC 361 AATCCATCCC GGATACGGCT TCCTTGCAGA AAACGCGGAC TTCGCCGAAG CTGTTATAAA 421 CGAAGGGCTA ATTTGGATTG GCCCGTCCCC AGAGAGCATC AGATCCTTAG GAGACAAAGT 481 AACAGCGCGG CATATTGCGG ATACCGCAAA GGCGCCGATG GCACCCGGGA CGAAAGAGCC 541 CGTGAAAGAT GCCGCCGAAG TCGTGGCTTT TGCAGAGGAG TTTGGGCTGC CGATCGCTAT 601 CAAAGCAGCC TTTGGTGGTG GTGGCCGTGG AATGAAGGTG GCCTACAAAA TGGAAGAAGT 661 CGCGGATCTG TTTGAGTCTG CGACCCGTGA AGCGACCGCC GCTTTCGGTC GAGGTGAGTG 721 CTTTGTGGAA CGGTATCTGG ACAAAGCGCG CCACGTGGAA GCGCAAGTCA TCGCAGATAA 781 ACATGGAAAC GTCGTAGTGG CGGGCACCCG CGACTGCTCG CTTCAGCGCC GTTTTCAAAA 841 ACTCGTGGAA GAAGCGCCGG CACCGTTTCT CACTGATGAC CAAAGAGAGC GTTTACACTC 901 AAGTGCAAAG GCGATTTGTA AAGAAGCTGG CTATTATGGT GCTGGCACTG TTGAATACTT 961 GGTTGGTTCT GATGGACTCA TCTCGTTCCT GGAAGTGAAT ACCCGTCTGC AAGTCGAACA 1021 CCCTGTGACG GAAGAAACGA CAGGTATCGA CCTGGTTCGC GAAATGTTTC GTATTGCGGA 1081 AGGTCATGAA CTGTCTATTA AAGAAGATCC AGCACCCCGC GGCCACGCCT TCGAGTTCCG 1141 TATTAACGGC GAGGATGCCG GCTCAAACTT CATGCCGGCT CCTGGAAAGA TTACTAGCTA 1201 CCGAGAACCA CAGGGGCCTG GCGTTCGTAT GGACTCCGGC GTCGTGGAGG GGAGCGAAAT 1261 ATCTGGTCAG TTTGACAGTA TGTTGGCAAA GCTTATTGTC TGGGGCGATA CCCGCGAACA 1321 GGCCTTACAG CGCAGCCGTC GCGCCCTGGC GGAATATGTA GTGGAGGGTA TGCCGACCGT 1381 AATCCCGTTC CACCAGCATA TTGTGGAAAA TCCGGCCTTT GTTGGGAATG ATGAAGGTTT 1441 TGAAATTTAC ACCAAATGGA TAGAGGAAGT ATGGGACAAT CCTATTGCAC CTTATGTGGA 1501 TGCGTCCGAA CTGGATGAGG ATGAAGATAA AACGCCAGCG CAGAAAGTTG TTGTAGAAAT 1561 TAATGGCCGC CGCGTTGAGG TGGCGCTGCC GGGCGATCTG GCACTGGGCG GGACAGCGGG 1621 ACCGAAAAAA AAGGCTAAAA AACGGAGGGC GGGCGGTGCG AAAGCAGGCG TCAGTGGGGA 1681 TGCGGTAGCG GCACCGATGC AGGGTACGGT GATTAAAGTA AATGTCGAGG AAGGCGCAGA 1741 GGTGAACGAA GGTGATACCG TGGTTGTACT TGAAGCAATG AAGATGGAAA ACCCGGTGAA 1801 AGCCCATAAA TCAGGCACGG TGACGGGTTT GACCGTTGCC GCGGGTGAAG GCGTCAATAA 1861 AGGTGTGGTT CTGTTAGAGA TTAAATAAcc aggcatcaaa taaaacgaaa ggctcagtcg 1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 1981 actggctcac cttcgggtgg gcctttctgc gtttatatag tAGAGACCAG GTGC
//`
sequences.seq_ACC_outro_gblock = {}; sequences.seq_ACC_outro_gblock.varName = "seq_ACC_outro_gblock"; sequences.seq_ACC_outro_gblock.name = "ACC outro gblock"; sequences.seq_ACC_outro_gblock.seqType = "both"; sequences.seq_ACC_outro_gblock.data = `LOCUS ACC_outro_gblock 2216 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 2179..2199 /label="Suffix" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 388..417 /label="Spacer" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 18..52 /label="Promoter" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2050..2178 /label="Terminator" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 418..2049 /label="ACCbeta" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 77..90 /label="RBS" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 94..387 /label="ZFA" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_binding 53..76 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 CCAGCCGGTC TCATAGTttg acggctagct cagtcctagg tatagtgcta gcTGGAATTG 61 TGAGCGGATA ACAATTGAAA GAGGTGACAT ATGACATCTG CAGCGGCCCC GGGGGAACGG 121 CCATTCCAGT GTCGGATTTG CATGAGAAAC TTTAGCGATT CACCGACCCT GCGTCGTCAC 181 ACCCGCACGC ATACTGGCGA AAAGCCTTTC CAGTGTCGCA TTTGCATGCG CAATTTTTCC 241 GTTCGTCATA ATTTAACGCG CCACCTTCGC ACGCACACCG GTGAGAAACC GTTCCAATGC 301 CGTATCTGTA TGCGTAACTT TTCGGACCGC ACTAGTCTGG CGCGACATTT GAAAACCCAT 361 TATCCCTATG ATGTGCCGGA TTACGCTggt ggaagtggag gtggaagtgg aggtagtATG 421 ACGATCAGTT CCCCGCTGAT CGATGTTGCT AACCTGCCGG ATATTAACAC CACGGCGGGA 481 AAAATAGCTG ATCTCAAAGC GCGCCGCGCG GAAGCCCATT TCCCTATGGG CGAAAAGGCG 541 GTTGAGAAAG TGCACGCCGC AGGTCGTCTG ACAGCACGGG AACGTCTGGA TTATCTCTTA 601 GATGAAGGCT CTTTCATCGA AACCGATCAA CTTGCCCGCC ACCGGACGAC GGCGTTTGGC 661 CTGGGCGCCA AACGTCCGGC AACCGATGGG ATCGTGACCG GGTGGGGTAC CATTGATGGC 721 CGCGAAGTCT GTATTTTCAG CCAGGATGGA ACTGTTTTTG GCGGCGCGCT GGGCGAGGTT 781 TACGGGGAAA AAATGATAAA AATTATGGAA TTAGCGATCG ATACGGGGCG TCCGCTGATT 841 GGCCTGTACG AGGGAGCGGG CGCCCGAATC CAGGATGGTG CAGTCTCGCT AGATTTCATT 901 AGTCAAACAT TTTACCAGAA TATCCAGGCC AGCGGAGTAA TTCCCCAGAT TAGTGTGATC 961 ATGGGCGCGT GCGCCGGTGG GAATGCTTAT GGCCCAGCGT TGACAGACTT CGTGGTGATG 1021 GTCGATAAGA CGTCTAAAAT GTTTGTGACG GGTCCAGATG TGATTAAGAC CGTGACTGGC 1081 GAGGAAATTA CCCAGGAGGA GCTTGGTGGT GCAACGACAC ACATGGTAAC TGCGGGAAAC 1141 TCCCATTATA CTGCGGCTAC CGACGAGGAA GCCTTGGATT GGGTTCAAGA TCTGGTTAGC 1201 TTTCTGCCTT CCAACAACCG GTCTTACACT CCCCTGGAAG ATTTTGACGA AGAAGAAGGT 1261 GGGGTTGAAG AAAATATCAC CGCAGACGAC CTTAAACTCG ACGAAATCAT TCCGGATTCA 1321 GCTACCGTAC CATACGACGT TCGTGACGTC ATCGAATGTC TTACCGATGA CGGTGAGTAT 1381 TTGGAGATTC AGGCGGACCG CGCCGAAAAC GTCGTGATTG CGTTTGGGCG CATTGAAGGT 1441 CAAAGCGTTG GCTTTGTGGC AAATCAGCCG ACTCAGTTCG CGGGTTGTCT GGATATTGAT 1501 TCATCGGAAA AAGCAGCACG TTTCGTGCGT ACGTGCGATG CGTTTAACAT CCCGATTGTA 1561 ATGCTGGTCG ACGTGCCGGG ATTTCTGCCG GGAGCCGGTC AAGAATATGG TGGTATTCTG 1621 AGACGCGGCG CTAAATTATT ATATGCATAT GGCGAAGCAA CAGTACCGAA AATCACCGTT 1681 ACCATGCGTA AGGCATACGG GGGTGCGTAC TGCGTCATGG GCTCAAAAGG CTTGGGCAGT 1741 GACATCAATC TAGCGTGGCC GACCGCTCAG ATTGCCGTGA TGGGTGCCGC GGGCGCAGTC 1801 GGCTTCATAT ATCGCAAAGA ACTGATGGCG GCCGATGCCA AAGGTCTGGA TACGGTGGCT 1861 CTGGCTAAAA GCTTTGAGCG CGAGTATGAA GACCACATGC TGAATCCTTA TCATGCTGCC 1921 GAGCGTGGGC TCATAGACGC GGTAATCTTA CCCTCGGAAA CCAGGGGCCA GATTAGCCGA 1981 AATCTGCGTC TTCTGAAGCA TAAAAATGTG ACACGCCCAG CCAGAAAACA TGGTAACATG 2041 CCTTTGTAAc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2101 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2161 ggcctttctg cgtttatata ctagtagcgg ccgctgcagT GCCAGAGACC CCATTA
//`
sequences.seq_ACS_intro_gblock = {}; sequences.seq_ACS_intro_gblock.varName = "seq_ACS_intro_gblock"; sequences.seq_ACS_intro_gblock.name = "ACS intro gblock"; sequences.seq_ACS_intro_gblock.seqType = "linear"; sequences.seq_ACS_intro_gblock.data = `LOCUS ACS_intro_gblock 458 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 18..39 /label="Prefix" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 40..74 /label="promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 122..415 /label="ZFB" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_binding 75..98 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 99..118 /label="RBS" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 416..445 /label="Spacer" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9
ORIGIN
1 GTTTCAGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta 61 ggtacagtgc tagcTGGAAT TGTGAGCGGA TAACAATTTC GACTCGTTCG ATTAAAGAAT 121 GACCAGTGCG GCTGCCCCTG GTGAACGCCC GTTTCAGTGT CGGATTTGCA TGCGAAATTT 181 CAGCAAAAAA GATCATCTCC ACCGTCATAC TCGCACACAC ACTGGGGAAA AACCGTTCCA 241 GTGTCGCATT TGCATGCGTA ACTTTTCCTT GTCACAAACC CTGAAACGTC ACCTTCGCAC 301 GCATACGGGC GAGAAGCCCT TCCAGTGTAG AATCTGCATG CGCAACTTTT CGCGTTTAGA 361 TATGCTGGCG CGTCATCTGA AAACCCATTA TCCATACGAT GTGCCGGACT ATGCAggtgg 421 aagtggaggt ggaagtggag gtagtAGAGA CCGGACTA
//`
sequences.seq_ACS_outro_gblock = {}; sequences.seq_ACS_outro_gblock.varName = "seq_ACS_outro_gblock"; sequences.seq_ACS_outro_gblock.name = "ACS outro gblock"; sequences.seq_ACS_outro_gblock.seqType = "linear"; sequences.seq_ACS_outro_gblock.data = `LOCUS ACS_outro_gblock 2143 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 2106..2126 /label="Suffix" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 18..1973 /label="ACS" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1977..2105 /label="Terminator" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 GTTTCAGGTC TCATAGTATG TCTCAGATTC ACAAACATAC TATCCCTGCA AACATTGCTG 61 ACCGTTGCCT GATTAATCCT CAACAGTATG AAGCCATGTA CCAGCAGTCG ATTAATGTGC 121 CGGATACATT TTGGGGCGAA CAGGGGAAGA TTCTGGACTG GATCAAACCG TACCAAAAAG 181 TGAAAAATAC CAGTTTCGCA CCGGGTAATG TTAGCATCAA ATGGTACGAA GACGGCACTC 241 TTAACCTTGC GGCGAATTGT CTGGATCGTC ATCTACAAGA AAACGGCGAT CGTACAGCGA 301 TTATATGGGA GGGTGATGAT GCTAGCCAGT CGAAGCATAT CAGCTATAAA GAACTTCATC 361 GCGACGTATG CCGCTTCGCA AACACGTTGT TAGAATTAGG CATCAAGAAA GGTGATGTGG 421 TCGCCATTTA TATGCCAATG GTTCCGGAAG CGGCCGTGGC TATGCTCGCA TGCGCCCGTA 481 TTGGAGCGGT CCATTCGGTT ATTTTTGGGG GGTTTTCCCC GGAGGCAGTC GCCGGCCGCA 541 TTATTGATTC CAATAGCCGT CTCGTCATCA CCTCTGATGA AGGAGTTCGG GCGGGCCGCA 601 GCATTCCTCT GAAAAAGAAT GTAGACGATG CTCTGAAAAA CCCTAACGTC ACGAGTGTCG 661 AGCACGTTGT TGTGTTAAAA CGCACCGGTG GTAAAATTGA CTGGCAGGAA GGGCGTGACT 721 TGTGGTGGCA TGACCTTGTA GAACAAGCGT CCGATCAGCA TCAGGCTGAA GAGATGAACG 781 CAGAGGACCC GCTGTTCATC TTATATACCA GCGGATCTAC AGGTAAACCC AAAGGGGTGC 841 TGCATACTAC GGGCGGTTAT TTGGTGTACG CCGCCCTGAC TTTTAAATAC GTCTTTGATT 901 ACCACCCCGG TGACATCTAT TGGTGTACCG CTGATGTCGG TTGGGTTACC GGACATAGCT 961 ATCTGCTGTA TGGCCCCTTG GCATGTGGGG CCACAACGTT GATGTTTGAA GGAGTGCCGA 1021 ATTGGCCGAC CCCAGCCCGC ATGGCGCAGG TAGTTGATAA GCATCAGGTG AACATCCTGT 1081 ACACGGCTCC GACGGCCATT CGAGCTCTGA TGGCGGAAGG CGATAAAGCG ATAGAAGGCA 1141 CGGATCGCTC TTCTCTCCGC ATCCTGGGTT CAGTTGGCGA GCCAATCAAC CCAGAGGCGT 1201 GGGAGTGGTA CTGGAAAAAA ATTGGTAATG AAAAATGCCC GGTCGTGGAC ACCTGGTGGC 1261 AGACCGAAAC GGGAGGCTTT ATGATTACCC CGCTGCCAGG CGCGACGGAG CTAAAGGCAG 1321 GGAGCGCAAC AAGGCCTTTT TTCGGTGTGC AACCGGCCTT AGTGGACAAT GAGGGTAATC 1381 CGCTGGAAGG GGCGACCGAG GGATCACTGG TGATTACTGA CAGTTGGCCG GGCCAGGCAC 1441 GAACCCTGTT CGGTGATCAC GAAAGATTCG AACAAACGTA TTTCTCCACA TTTAAAAACA 1501 TGTATTTTTC AGGTGACGGC GCGCGTAGAG ATGAAGATGG TTATTATTGG ATCACCGGCC 1561 GGGTCGATGA TGTGCTCAAC GTTAGCGGTC ACCGTCTTGG CACGGCGGAA ATCGAATCGG 1621 CTCTGGTGGC CCACCCTAAG ATCGCGGAGG CAGCCGTGGT AGGCATTCCG CATAACATAA 1681 AAGGCCAGGC CATTTACGCG TATGTGACTT TGAATCACGG CGAAGAACCA TCGCCGGAAT 1741 TATACGCGGA AGTACGCAAC TGGGTTCGGA AAGAAATCGG TCCACTGGCA ACCCCGGATG 1801 TACTGCACTG GACCGATTCA CTGCCCAAAA CGCGCAGTGG GAAGATCATG CGTCGCATAC 1861 TGCGTAAAAT TGCGGCCGGT GATACTTCAA ACCTCGGCGA TACCTCCACC CTTGCAGATC 1921 CCGGCGTAGT TGAAAAACTG CTGGAAGAGA AACAAGCCAT TGCGATGCCG AGTTGAccag 1981 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2041 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2101 ttatatacta gtagcggccg ctgcagTGCC AGAGACCGGA CTA
//`
sequences.seq_BsaI_FWD_primer = {}; sequences.seq_BsaI_FWD_primer.varName = "seq_BsaI_FWD_primer"; sequences.seq_BsaI_FWD_primer.name = "BsaI FWD primer"; sequences.seq_BsaI_FWD_primer.seqType = "linear"; sequences.seq_BsaI_FWD_primer.data = `LOCUS BsaI_FWD_primer 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CTCTCAGGTC TCATGCCagt ccggcaaaaa agggc
//`
sequences.seq_BsaI_REV_primer = {}; sequences.seq_BsaI_REV_primer.varName = "seq_BsaI_REV_primer"; sequences.seq_BsaI_REV_primer.name = "BsaI REV primer"; sequences.seq_BsaI_REV_primer.seqType = "linear"; sequences.seq_BsaI_REV_primer.data = `LOCUS BsaI_REV_primer 40 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATCTTAGGTC TCTCTCCcag aaatcatcct tagcgaaagc
//`
sequences.seq_CoA_4x_Assembly = {}; sequences.seq_CoA_4x_Assembly.varName = "seq_CoA_4x_Assembly"; sequences.seq_CoA_4x_Assembly.name = "CoA 4x Assembly"; sequences.seq_CoA_4x_Assembly.seqType = "both"; sequences.seq_CoA_4x_Assembly.data = `LOCUS CoA_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2480..2488 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2469..2477 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Zinc Finger Site 2458..2466 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 2447..2455 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2436..2444 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2414..2422 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2403..2411 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site 2425..2433 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 HIV-RT Binding Site join(2489..2492,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG 2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AA
//`
sequences.seq_CoA_4x_Comp_Assembly = {}; sequences.seq_CoA_4x_Comp_Assembly.varName = "seq_CoA_4x_Comp_Assembly"; sequences.seq_CoA_4x_Comp_Assembly.name = "CoA 4x Comp Assembly"; sequences.seq_CoA_4x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_4x_Comp_Assembly.data = `LOCUS CoA_4x_Comp_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2425..2433) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2480..2488) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2469..2477) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site complement(2447..2455) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Zinc Finger Site complement(2414..2422) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2403..2411) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2436..2444) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2392..2400) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 HIV-RT Binding Site join(2489..2492,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2458..2466) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC 2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC 2461 AGCCGCATGG CATCGACATG CCGTCCTCAA AA
//`
sequences.seq_CoA_4x_Full_Part = {}; sequences.seq_CoA_4x_Full_Part.varName = "seq_CoA_4x_Full_Part"; sequences.seq_CoA_4x_Full_Part.name = "CoA 4x Full Part"; sequences.seq_CoA_4x_Full_Part.seqType = "both"; sequences.seq_CoA_4x_Full_Part.data = `LOCUS CoA_4x_Full_Part 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2779..2787) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2425..2433 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2447..2455 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 HIV-RT Binding Site 2489..2598 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site 2436..2444 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 2414..2422 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2746..2754) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Scar 2668..2675 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2458..2466 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site join(2832..2835,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2735..2743) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2768..2776) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2790..2798) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2801..2809) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Promoter 2676..2710 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2812..2820) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2403..2411 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2480..2488 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac gBlock 2545..2669 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site 2469..2477 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2757..2765) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 2599..2667 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_binding 2712..2734 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2823..2831) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG 2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT 2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC 2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact 2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA 2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTCCCAGC CGCATGGCAT CGACATCCCA 2761 GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC 2821 ATGCCGTCCT CAAAA
//`
sequences.seq_CoA_4x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.varName = "seq_CoA_4x_r_oligo_Complement_Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.name = "CoA 4x r_oligo Complement Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.data = `LOCUS CoA_4x_r_oligo_Complemen 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(42..50) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(53..61) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(64..72) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(75..83) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(86..94) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(97..105) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(108..116) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(119..127) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(130..138) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(42..138) /label="Temp2" /note="sequence: GAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer 42..138 /label="Temp" /note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTC" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA 61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG 121 CATCGACATG CCGTCCTCAA AACGAGACCA ACCTG
//`
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.varName = "seq_CoA_4x_r_oligo_Duplex_126145_REV_1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.name = "CoA 4x r_oligo Duplex 126-145 REV 1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.data = `LOCUS CoA_4x_r_oligo_Duplex_126 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGTTTTCCC AGCCGCATGG
//`
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.varName = "seq_CoA_4x_r_oligo_Duplex_6988_FWD_1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.name = "CoA 4x r_oligo Duplex 69-88 FWD 1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.data = `LOCUS CoA_4x_r_oligo_Duplex_69- 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TGGGATGTCG ATGCCATGCG
//`
sequences.seq_CoA_4x_r_oligo_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Duplex.varName = "seq_CoA_4x_r_oligo_Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.name = "CoA 4x r_oligo Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex.data = `LOCUS CoA_4x_r_oligo_Duplex 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 42..50 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site 53..61 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 64..72 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 75..83 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 86..94 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 97..105 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 108..116 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 119..127 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 130..138 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(126..145) /label="CoA 4x r_oligo Duplex 126-145 REV 1" /note="sequence: TCGTTTTCCCAGCCGCATGG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 18..138 /label="Temp Fwd" /note="sequence: TGGAATTGTGAGCGGATAACAATTGAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(20..140) /label="Temp Rev" /note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTCAATTGTTATCCGCTCACAATTCCA" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer 69..88 /label="CoA 4x r_oligo Duplex 69-88 FWD 1" /note="sequence: TGGGATGTCGATGCCATGCG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC 61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT 121 CGATGCCATG CGGCTGGGAA AACGAGACCA ACCTG
//`
sequences.seq_CoA_6x_Assembly = {}; sequences.seq_CoA_6x_Assembly.varName = "seq_CoA_6x_Assembly"; sequences.seq_CoA_6x_Assembly.name = "CoA 6x Assembly"; sequences.seq_CoA_6x_Assembly.seqType = "both"; sequences.seq_CoA_6x_Assembly.data = `LOCUS CoA_6x_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2502..2510 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site 2491..2499 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2480..2488 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2524..2532 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2469..2477 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2458..2466 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2513..2521 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2447..2455 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2436..2444 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2425..2433 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2414..2422 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2403..2411 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 HIV-RT Binding Site join(2533..2536,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG 2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC 2521 CATGCGGCTG GGAAAA
//`
sequences.seq_CoA_6x_Comp_Assembly = {}; sequences.seq_CoA_6x_Comp_Assembly.varName = "seq_CoA_6x_Comp_Assembly"; sequences.seq_CoA_6x_Comp_Assembly.name = "CoA 6x Comp Assembly"; sequences.seq_CoA_6x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_6x_Comp_Assembly.data = `LOCUS CoA_6x_Comp_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site complement(2491..2499) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2502..2510) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2480..2488) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2458..2466) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2447..2455) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site complement(2436..2444) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2425..2433) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2414..2422) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2403..2411) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2392..2400) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2513..2521) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2524..2532) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 HIV-RT Binding Site join(2533..2536,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site complement(2469..2477) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC 2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC 2461 AGCCGCATGG CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA 2521 CATGCCGTCC TCAAAA
//`
sequences.seq_CoA_6x_Full_Part = {}; sequences.seq_CoA_6x_Full_Part.varName = "seq_CoA_6x_Full_Part"; sequences.seq_CoA_6x_Full_Part.name = "CoA 6x Full Part"; sequences.seq_CoA_6x_Full_Part.seqType = "both"; sequences.seq_CoA_6x_Full_Part.data = `LOCUS CoA_6x_Full_Part 2923 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site 2425..2433 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_binding 2756..2778 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2790..2798) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2469..2477 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2900..2908) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2480..2488 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2447..2455 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2867..2875) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site 2533..2642 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2524..2532 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site complement(2823..2831) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2458..2466 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2812..2820) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2436..2444 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site complement(2878..2886) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site 2491..2499 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2856..2864) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2502..2510 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Terminator 2643..2711 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2834..2842) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2513..2521 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(2779..2787) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Zinc Finger Site 2403..2411 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Promoter 2720..2754 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2845..2853) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Scar 2712..2719 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2414..2422 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 gBlock 2589..2713 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2889..2897) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2801..2809) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site join(2920..2923,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2911..2919) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG 2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC 2521 CATGCGGCTG GGAAAAAAAA CGTGGCGCCC GAACAGGGAC GGATAGCTCA GTCGGTAGAG 2581 CATCAGACTT TTAATCTGAG GGTCCAGGGT TCAAGTCCCT GTTCGGGCGC CACGTTTTTT 2641 TTattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact tcccttgggg 2701 ttatcattgg gtactagagT TGACGGCTAG CTCAGTCCTA GGTACAGTGC TAGCTGGAAT 2761 TGTGAGCGGA TAACAATTCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC 2821 ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT CGACATCCCA GCCGCATGGC 2881 ATCGACATCC CAGCCGCATG GCATCGACAT GCCGTCCTCA AAA
//`
sequences.seq_CoA_6x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.varName = "seq_CoA_6x_r_oligo_Complement_Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.name = "CoA 6x r_oligo Complement Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.data = `LOCUS CoA_6x_r_oligo_Complemen 199 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(42..50) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(53..61) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(64..72) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(75..83) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(86..94) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(97..105) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(108..116) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(119..127) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(130..138) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(141..149) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(152..160) /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(163..171) /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site complement(174..182) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA 61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG 121 CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA CATGCCGTCC 181 TCAAAACGAG ACCAACCTG
//`
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.varName = "seq_CoA_6x_r_oligo_Duplex_113132_FWD_1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.name = "CoA 6x r_oligo Duplex 113-132 FWD 1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.data = `LOCUS CoA_6x_r_oligo_Duplex_113 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TGGGATGTCG ATGCCATGCG
//`
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.varName = "seq_CoA_6x_r_oligo_Duplex_170189_REV_1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.name = "CoA 6x r_oligo Duplex 170-189 REV 1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.data = `LOCUS CoA_6x_r_oligo_Duplex_170 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGTTTTCCC AGCCGCATGG
//`
sequences.seq_CoA_6x_r_oligo_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Duplex.varName = "seq_CoA_6x_r_oligo_Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.name = "CoA 6x r_oligo Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex.data = `LOCUS CoA_6x_r_oligo_Duplex 199 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 42..50 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site 53..61 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 64..72 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 75..83 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 86..94 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 97..105 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 108..116 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 119..127 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 130..138 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 141..149 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 152..160 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 163..171 /label="ZFa Site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 174..182 /label="ZFb Site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer 113..132 /label="CoA 6x r_oligo Duplex 113-132 FWD 1" /note="sequence: TGGGATGTCGATGCCATGCG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(170..189) /label="CoA 6x r_oligo Duplex 170-189 REV 1" /note="sequence: TCGTTTTCCCAGCCGCATGG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC 61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT 121 CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG 181 GGAAAACGAG ACCAACCTG
//`
sequences.seq_gBlock_A = {}; sequences.seq_gBlock_A.varName = "seq_gBlock_A"; sequences.seq_gBlock_A.name = "gBlock A"; sequences.seq_gBlock_A.seqType = "linear"; sequences.seq_gBlock_A.data = `LOCUS gBlock_A 995 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 863..870 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 785..790 /label="double stop codon" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 18..979 /label="gBlock A" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e stem_loop 883..905 /label="stem loop" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 110..815 /label="RFP" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 82..89 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 97..101 /label="strong RBS" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 110..815 /label="BBa_E1010 (RFP)" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 110..112 /label="start codon" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 90..104 /label="BBa_B0030 (strong RBS)" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 822..862 /label="BBa_B0012" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 822..916 /label="BBa_B0014 (double terminator)" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 871..916 /label="BBa_B0011" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 18..39 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 90..916 /label="From BBa_K3114015" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 794..799 /label="double stop codon" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 40..74 /label="BBa_J23105" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 GACTTACGTC TCGtctggaa ttcgcggccg cttctagagt ttacggctag ctcagtccta 61 ggtactatgc tagcCGAGAC Ctactagaga ttaaagagga gaaatactaa tggcttcctc 121 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg 181 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc 241 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca 301 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa 361 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt 421 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact 481 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga 541 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg 601 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa 661 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca 721 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg 781 tgcttaataa cgctgatagt gctagtgtag atcgctaata atcacactgg ctcaccttcg 841 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc 901 ggctttttta ttatttGGTC TCGAAAAAAA ACGTGGCGCC CGAACAGGGA CGGATAGCTC 961 AGTCGGTAGA GCATCAGACT TTCGAGACGC TTGTG
//`
sequences.seq_gBlock_B = {}; sequences.seq_gBlock_B.varName = "seq_gBlock_B"; sequences.seq_gBlock_B.name = "gBlock B"; sequences.seq_gBlock_B.seqType = "linear"; sequences.seq_gBlock_B.data = `LOCUS gBlock_B 175 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 138..158 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 15..158 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc 72..111 /label="identical to E. coli MG1655 between tonB and yciA" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 69..137 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 stem_loop 79..110 /label="stem loop" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 GATCGTCGTC TCCCTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC GGGCGCCACG 61 TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc 121 ttggggttat cattgggtac tagtagcggc cgctgcagag tcCGAGACGT TCCAT
//`
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear = {}; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.varName = "seq_GFP_final_synthesized_for_pSB1T3_linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.name = "GFP final synthesized for pSB1T3 linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.seqType = "linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.data = `LOCUS GFP_final_synthesized_f 1240 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 91..405 /label="Zif268 + linker" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Recognition Sequence 7..12 /label="BsaI 5' site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1..6 /label="rando bases" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1235..1240 /label="rando bases" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 18..52 /label="new promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 406..1122 /label="GFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Recognition Sequence 1229..1234 /label="BsaI 3' site" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Scar 1170..1177 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1129..1223 /label="BBa_B0014 Double Terminator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 77..90 /label="RBS - J61111" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_binding 54..76 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 GATACGGGTC TCACGTTttt acggctagct cagtcctagg tatagtgcta gcTGGAATTG 61 TGAGCGGATA ACAATTGAAA GAGGCGATAC ATGCACCATC ACCATCATCA CCCGGGTGAG 121 AAGCCATATG CCTGCCCCGT GGAAAGCTGC GATCGTCGTT TCAGTCGCTC TGATGAACTG 181 ACACGCCACA TCAGAATACA TACCGGGCAG AAACCGTTTC AATGTCGCAT TTGTATGCGC 241 AACTTTTCGC GCAGCGACCA TTTAACTACG CATATCCGTA CCCACACTGG TGAAAAACCT 301 TTCGCGTGTG ATATTTGCGG ACGAAAATTT GCACGGTCAG ACGAGCGTAA ACGTCATACC 361 AAAATTCATA CGGGCGGCGG CAGCGGCGGC GGTAGTGGTG GGTCCcgtaa aggagaagaa 421 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa 481 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt 541 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat 601 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt 661 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac 721 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa 781 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac 841 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa 901 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact 961 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc 1021 ctttcgaaag atcccaacga aaagAGGgac cacatggtcc ttcttgagtt tgtaacagct 1081 gctgggatta cacatggcat ggatgaacta tacaaataat aatggctgtc acactggctc 1141 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta 1201 ttaatccggc ttttttatta tttTGCCaGA GACCAGGCTT
//`
sequences.seq_GFP_Fusion_in_TetR = {}; sequences.seq_GFP_Fusion_in_TetR.varName = "seq_GFP_Fusion_in_TetR"; sequences.seq_GFP_Fusion_in_TetR.name = "GFP Fusion in TetR"; sequences.seq_GFP_Fusion_in_TetR.seqType = "both"; sequences.seq_GFP_Fusion_in_TetR.data = `LOCUS GFP_Fusion_in_TetR 3675 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 2539..2853 /label="Zif268 + linker" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2466..2500 /label="new promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 terminator 2394..2437 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2525..2538 /label="RBS - J61111" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd primer_bind complement(103..120) /label="L4440" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Biobricking 2440..2461 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 3672..3675 /label="3' overhang" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Scar 3618..3625 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 22..79 /label="his operon terminator" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 3577..3671 /label="BBa_B0014 Double Terminator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Biobricking 1..21 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2854..3570 /label="GFP" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 1..21 /label="BioBrick suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 2372..2390 /label="pBRforEco" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 rep_origin complement(274..862) /label="ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 2462..2465 /label="5' overhang" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind complement(354..373) /label="pBR322ori-F" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS 1128..2312 /label="TetR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2440..2461 /label="BioBrick prefix" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer complement(2444..2461) /label="Copy of pSB1T3 REV" /note="sequence: AAGGTCGGTCTCCCTCTctctagaagcggccgcga" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 1..18 /label="Copy of pSB1T3 FWD" /note="sequence: AAGGTCGGTCTCCTACTtactagtagcggccgctg" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt 61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct 121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga 301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1021 ttggtctgac agctcgagat tctcatgttt gacagcttat catcgataag ctttaatgcg 1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg 1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc 1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg 1261 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac 1321 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg 1381 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg 1441 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg 1501 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg 1561 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg 1621 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata 1681 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 1741 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag 1801 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga 1861 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg 1921 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg 1981 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca 2041 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca 2101 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc 2161 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat 2221 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc 2281 gtcgcggtgc atggagccgg gccacctcga cctaactcga gtgccacctg acgtctaaga 2341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag 2401 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga 2461 gAGAGtttac ggctagctca gtcctaggta tagtgctagc TGGAATTGTG AGCGGATAAC 2521 AATTGAAAGA GGCGATACAT GCACCATCAC CATCATCACC CGGGTGAGAA GCCATATGCC 2581 TGCCCCGTGG AAAGCTGCGA TCGTCGTTTC AGTCGCTCTG ATGAACTGAC ACGCCACATC 2641 AGAATACATA CCGGGCAGAA ACCGTTTCAA TGTCGCATTT GTATGCGCAA CTTTTCGCGC 2701 AGCGACCATT TAACTACGCA TATCCGTACC CACACTGGTG AAAAACCTTT CGCGTGTGAT 2761 ATTTGCGGAC GAAAATTTGC ACGGTCAGAC GAGCGTAAAC GTCATACCAA AATTCATACG 2821 GGCGGCGGCA GCGGCGGCGG TAGTGGTGGG TCCcgtaaag gagaagaact tttcactgga 2881 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt 2941 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact 3001 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc 3061 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa 3121 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct 3181 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt 3241 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta 3301 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac 3361 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat 3421 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat 3481 cccaacgaaa agAGGgacca catggtcctt cttgagtttg taacagctgc tgggattaca 3541 catggcatgg atgaactata caaataataa tggctgtcac actggctcac cttcgggtgg 3601 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt 3661 ttttattatt tTACT
//`
sequences.seq_GGE114 = {}; sequences.seq_GGE114.varName = "seq_GGE114"; sequences.seq_GGE114.name = "GGE114"; sequences.seq_GGE114.seqType = "both"; sequences.seq_GGE114.data = `LOCUS (Addgene_#120731_)_GGE1 5268 bp ds-DNA circular 06-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
terminator 4070..4113 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2002..4115 /label="pSB1A3 Region" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e protein_bind 4452..4485 /label="loxP" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 source 1..5268 /label="source" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind 3638..3657 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac promoter complement(3876..3980) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS 722..1399 /label="mRFP1" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS complement(3015..3875) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 rep_origin complement(2256..2844) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 promoter 632..662 /label="lac promoter" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(2085..2102) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 terminator 2004..2061 /label="his operon terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 protein_bind 670..686 /label="lac operator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 protein_bind 596..617 /label="CAP binding site" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 terminator 1444..1515 /label="rrnB T1 terminator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind 4048..4066 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(2336..2355) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 3156..3156 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 1531..1558 /label="T7Te terminator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 RBS 704..715 /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer 2002..2019 /label="Linear pSB1A3 BsmBI Fwd" /note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(4088..4115) /label="BBa_Prefix & Promoter SDM Rev" /note="sequence: cagaaatcatccttagcgaaagctaagg" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer complement(4093..4115) /label="BsaI pSB1A3 Rev" /note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 4116..4135 /label="BBa_Prefix & Promoter SDM Fwd" /note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 2002..2019 /label="BsaI pSB1A3 Fwd" /note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(4093..4115) /label="Linear pSB1A3 BsmBI Rev" /note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(4093..4115) /label="pSB1A3 SapI rev" /note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 2002..2019 /label="pSB1A3 fwd SapI" /note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 2002..2023 /label="Linear pSB1A3 BsmBI Fwd" /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295
ORIGIN
1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc 61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag 121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc 181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc 241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt 301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg 361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc 421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag 481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta 721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc 1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg 1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg 1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt 1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt 1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac 1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt 1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct 2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac 2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat 3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct 3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg 4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta 4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt 4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga 4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag 4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt 4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa 4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac 4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca 4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt 4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt 4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc 4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt 4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac 4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag 4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg 5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt 5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg 5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg 5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
//`
sequences.seq_Linear_pSB1A3_BsmBI_Fwd = {}; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.varName = "seq_Linear_pSB1A3_BsmBI_Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.name = "Linear pSB1A3 BsmBI Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.data = `LOCUS Linear_pSB1A3_BsmBI_Fwd 31 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CTATTTCGTC TCCagtccgg caaaaaaggg c
//`
sequences.seq_Linear_pSB1A3_BsmBI_Rev = {}; sequences.seq_Linear_pSB1A3_BsmBI_Rev.varName = "seq_Linear_pSB1A3_BsmBI_Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.name = "Linear pSB1A3 BsmBI Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.data = `LOCUS Linear_pSB1A3_BsmBI_Rev 36 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATTCCCCGTC TCCcagaaat catccttagc gaaagc
//`
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites = {}; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.varName = "seq_Linear_pSB1A3_with_BsmBI_Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.name = "Linear pSB1A3 with BsmBI Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.seqType = "linear"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.data = `LOCUS Linearized_pSB1A3_w/_Bs 2140 bp ds-DNA linear 24-JUN-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
CDS complement(1027..1887) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind 1650..1669 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac primer_bind complement(97..114) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer_bind 2060..2078 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer_bind complement(348..367) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 14..2127 /label="pSB1A3 Region" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1168..1168 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 2082..2125 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 terminator 16..73 /label="his operon terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter complement(1888..1992) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 rep_origin complement(268..856) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer complement(2100..2127) /label="BBa_Prefix & Promoter SDM Rev" /note="sequence: cagaaatcatccttagcgaaagctaagg" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 14..35 /label="Linear pSB1A3 BsmBI Fwd" /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295
ORIGIN
1 CTATTTCGTC TCCagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt 61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc 121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 241 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca 301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 1141 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc 1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 1741 gagatccagt tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt 1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 1981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 2041 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc 2101 cttagctttc gctaaggatg atttctgGGA GACGGGGAAT
//`
sequences.seq_pAC4CL1_FWD = {}; sequences.seq_pAC4CL1_FWD.varName = "seq_pAC4CL1_FWD"; sequences.seq_pAC4CL1_FWD.name = "pAC-4CL1 FWD"; sequences.seq_pAC4CL1_FWD.seqType = "linear"; sequences.seq_pAC4CL1_FWD.data = `LOCUS pAC-4CL1_FWD 39 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTCCCAGCTC TTCCagtatg gaggaggatt acaaaatgg
//`
sequences.seq_pAC4CL1_REV = {}; sequences.seq_pAC4CL1_REV.varName = "seq_pAC4CL1_REV"; sequences.seq_pAC4CL1_REV.name = "pAC-4CL1 REV"; sequences.seq_pAC4CL1_REV.seqType = "linear"; sequences.seq_pAC4CL1_REV.data = `LOCUS pAC-4CL1_REV 39 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTCCCAGCTC TTCCcaatca caatccattt gctagtttt
//`
sequences.seq_pAC4CL1 = {}; sequences.seq_pAC4CL1.varName = "seq_pAC4CL1"; sequences.seq_pAC4CL1.name = "pAC-4CL1"; sequences.seq_pAC4CL1.seqType = "both"; sequences.seq_pAC4CL1.data = `LOCUS pAC-4CL1 6156 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "marker:CmR" "ref:pmid\:16551366" FEATURES Location/Qualifiers
CDS complement(join(5716..6156,1..219)) /label="CmR" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter 1969..1975 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 protein_bind 1909..1930 /label="CAP binding site" /ApEinfo_revcolor=#31849b /ApEinfo_fwdcolor=#31849b CDS complement(join(5719..6156,1..219)) /label="CmR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter 1504..1509 /label="tet promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 rep_origin complement(848..1392) /label="p15A ori" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter 1951..1968 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2016..3719 /label="4CL" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd promoter 1527..1532 /label="tet promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 1945..1950 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter complement(220..322) /label="cat promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 1510..1526 /label="tet promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 1945..1975 /label="lac promoter" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt 61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt 121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga 181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga 241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt 301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc 361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat 421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt 481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg 541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact 601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa 661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc 721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc 781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa 841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc 901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct 961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg 1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact 1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg 1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta 1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt 1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc 1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca 1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttcaagat ttcagtgcaa 1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca 1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta 1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt 1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga 1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt 1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc 1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc 1861 cgtcctgtgg atccccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 1921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 1981 aattgtgagc gtctagagcg cccggggaat tccccatgga ggaggattac aaaatggcgc 2041 cacaagaaca agcagtttct caggtgatgg agaaacagag caacaacaac aacagtgacg 2101 tcattttccg atcaaagtta ccggatattt acatcccgaa ccacctatct ctccacgact 2161 acatcttcca aaacatctcc gaattcgcca ctaagccttg cctaatcaac ggaccaaccg 2221 gccacgtgta cacttactcc gacgtccacg tcatctcccg ccaaatcgcc gccaattttc 2281 acaaactcgg cgttaaccaa aacgacgtcg tcatgctcct cctcccaaac tgtcccgaat 2341 tcgtcctctc tttcctcgcc gcctccttcc gcggcgcaac cgccaccgcc gcaaaccctt 2401 tcttcactcc ggcggagata gctaaacaag ccaaagcctc caacaccaaa ctcataatca 2461 ccgaagctcg ttacgtcgac aaaatcaaac cacttcaaaa cgacgacgga gtagtcatcg 2521 tctgcatcga cgacaacgaa tccgtgccaa tccctgaagg ctgcctccgc ttcaccgagt 2581 tgactcagtc gacaaccgag gcatcagaag tcatcgactc ggtggagatt tcaccggacg 2641 acgtggtggc actaccttac tcctctggca cgacgggatt accaaaagga gtgatgctga 2701 ctcacaaggg actagtcacg agcgttgctc agcaagtcga cggcgagaac ccgaatcttt 2761 atttccacag cgatgacgtc atactctgtg ttttgcccat gtttcatatc tacgctttga 2821 actcgatcat gttgtgtggt cttagagttg gtgcggcgat tctgataatg ccgaagtttg 2881 agatcaatct gctattggag ctgatccaga ggtgtaaagt gacggtggct ccgatggttc 2941 cgccgattgt gttggccatt gcgaagtctt cggagacgga gaagtatgat ttgagctcga 3001 taagagtggt gaaatctggt gctgctcctc ttggtaaaga acttgaagat gccgttaatg 3061 ccaagtttcc taatgccaaa ctcggtcagg gatacggaat gacggaagca ggtccagtgc 3121 tagcaatgtc gttaggtttt gcaaaggaac cttttccggt taagtcagga gcttgtggta 3181 ctgttgtaag aaatgctgag atgaaaatag ttgatccaga caccggagat tctctttcga 3241 ggaatcaacc cggtgagatt tgtattcgtg gtcaccagat catgaaaggt tacctcaaca 3301 atccggcagc tacagcagag accattgata aagacggttg gcttcatact ggagatattg 3361 gattgatcga tgacgatgac gagcttttca tcgttgatcg attgaaagaa cttatcaagt 3421 ataaaggttt tcaggtagct ccggctgagc tagaggcttt gctcatcggt catcctgaca 3481 ttactgatgt tgctgttgtc gcaatgaaag aagaagcagc tggtgaagtt cctgttgcat 3541 ttgtggtgaa atcgaaggat tcggagttat cagaagatga tgtgaagcaa ttcgtgtcga 3601 aacaggttgt gttttacaag agaatcaaca aagtgttctt cactgaatcc attcctaaag 3661 ctccatcagg gaagatattg aggaaagatc tgagggcaaa actagcaaat ggattgtgac 3721 catgggcggc cgctgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 3781 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg 3841 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga 3901 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca 3961 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg 4021 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct 4081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 4141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg 4201 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa 4261 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga 4321 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg 4381 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga 4441 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc 4501 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg 4561 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc 4621 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct 4681 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca 4741 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc 4801 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt 4861 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg 4921 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc 4981 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac 5041 gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga 5101 taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt 5161 acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt 5221 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc 5281 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac 5341 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc 5401 tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata 5461 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag 5521 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg 5581 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc 5641 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata 5701 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt 5761 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg 5821 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa 5881 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga 5941 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca 6001 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca 6061 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc 6121 ccatatcacc agctcaccgt ctttcattgc catacg
//`
sequences.seq_pduABJKNUT_Fwd = {}; sequences.seq_pduABJKNUT_Fwd.varName = "seq_pduABJKNUT_Fwd"; sequences.seq_pduABJKNUT_Fwd.name = "pduABJKNUT Fwd"; sequences.seq_pduABJKNUT_Fwd.seqType = "linear"; sequences.seq_pduABJKNUT_Fwd.data = `LOCUS pduABJKNUT_Fwd 46 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCTGCTCGTC TCTgaccaca acggtttccc tgtagaaata attttg
//`
sequences.seq_pduABJKNUT_promoter = {}; sequences.seq_pduABJKNUT_promoter.varName = "seq_pduABJKNUT_promoter"; sequences.seq_pduABJKNUT_promoter.name = "pduABJKNUT promoter"; sequences.seq_pduABJKNUT_promoter.seqType = "linear"; sequences.seq_pduABJKNUT_promoter.data = `LOCUS *pduABJKNUT_promoter 133 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 38..72 /label="BBa_J23114" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 34..37 /label="Overhang pSB1C3" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 97..100 /label="Overhang pduABJKNUT" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_binding 74..96 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 AAGTGTTCGA ACGCTTCAGA TCTGTACGTC TCACAACttt atggctagct cagtcctagg 61 tacaatgcta gcTGGAATTG TGAGCGGATA ACAATTGACC AGAGACGTCT GCTTGCCAAC 121 CGTAGAACTT ACG
//`
sequences.seq_pduABJKNUT_Rev = {}; sequences.seq_pduABJKNUT_Rev.varName = "seq_pduABJKNUT_Rev"; sequences.seq_pduABJKNUT_Rev.name = "pduABJKNUT Rev"; sequences.seq_pduABJKNUT_Rev.seqType = "linear"; sequences.seq_pduABJKNUT_Rev.data = `LOCUS pduABJKNUT_Rev 42 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACTGTTCGTC TCAgctttgt tagcagccgg atccaaaagt ag
//`
sequences.seq_PduD_final_product = {}; sequences.seq_PduD_final_product.varName = "seq_PduD_final_product"; sequences.seq_PduD_final_product.name = "PduD (final product)"; sequences.seq_PduD_final_product.seqType = "linear"; sequences.seq_PduD_final_product.data = `LOCUS *PduD_(final_product) 658 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 115..154 /label="random bp" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 18..112 /label="BBa_B0014 Double Terminator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 653..658 /label="rando runway" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 218..231 /label="Anderson RBS 7" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Scar 59..66 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 583..621 /label="BBa_B1006" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1..6 /label="Rando Runway" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 286..582 /label="Spacer + ZFc" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 14..17 /label="5' overhang with pdu" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 159..193 /label="J23101 Anderson Promoter" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature 232..285 /label="pduD (1-18 aa)" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_binding 195..217 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 TGAGTACGTC TCAAAGCtca cactggctca ccttcgggtg ggcctttctg cgtttatata 61 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tttgAAGGGA 121 GTAAGTCTTC GAATGGTTCA GTTATAACTA TATAgctgtt tacagctagc tcagtcctag 181 gtattatgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGAGACT CATGGAAATC 241 AACGAAAAAC TGCTGCGTCA GATTATTGAG GACGTGTTAC GCGATACGTC GGCGGCCGCA 301 CCAGGCGAAC GCCCATTTCA GTGTCGCATC TGCATGCGTA ATTTCTCTTC ACCGAGCAAA 361 CTTATTCGCC ATACCCGTAC CCATACTGGC GAGAAACCCT TTCAGTGTCG TATCTGTATG 421 CGAAACTTCT CCGACGGGAG CAACCTGGCT CGGCACTTGC GTACACATAC CGGTGAAAAG 481 CCGTTTCAAT GCCGTATTTG CATGCGCAAT TTCAGTCGCG TGGATAACTT ACCGAGACAC 541 CTGAAAACGC ACTACCCGTA TGATGTTCCT GATTATGCGT AAaaaaaaaa accccgcccc 601 tgacagggcg gggttttttt tAATTAAAGA CACAAGTTTC CGTCAAGAGA CGTCGAGT
//`
sequences.seq_pduD_on_pSB1C3__Final_Part = {}; sequences.seq_pduD_on_pSB1C3__Final_Part.varName = "seq_pduD_on_pSB1C3__Final_Part"; sequences.seq_pduD_on_pSB1C3__Final_Part.name = "pduD on pSB1C3 - Final Part"; sequences.seq_pduD_on_pSB1C3__Final_Part.seqType = "both"; sequences.seq_pduD_on_pSB1C3__Final_Part.data = `LOCUS pduD_on_pSB1C3_-_Final_ 2589 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind complement(333..352) /label="pBR322ori-F" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Biobricking 2569..2584 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2028..2028 /label="BioBrick prefix" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 misc_feature 2177..2473 /label="Spacer + ZFc" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 primer_bind complement(82..99) /label="L4440" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 source 1..2028 /label="source" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2050..2084 /label="new promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 terminator 1982..2025 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Scar 2515..2522 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 rep_origin complement(253..841) /label="ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS complement(1139..1798) /label="CmR" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd misc_feature join(2585..2589,1..2028) /label="pSB1C3 Backbone Region" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_feature 2585..2589 /label="BioBrick suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc terminator 1..58 /label="his operon terminator" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer_bind 1713..1732 /label="CAT-R" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 misc_feature 2123..2176 /label="pduD (1-18 aa)" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Biobricking 2029..2049 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 1960..1978 /label="pBRforEco" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 2474..2568 /label="BBa_B0014 Double Terminator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Biobricking 2569..2589 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc terminator 1024..1118 /label="lambda t0 terminator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Biobricking 2028..2049 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc source 2585..2589 /label="source" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 misc_feature 2109..2122 /label="RBS-J61107" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt 61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc 301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct 1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg 1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt 1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat 1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc 1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca 1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct 1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat 1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt 1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac 1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa 1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat 1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt 1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt 1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta 1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac 1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag 1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg 2041 cttctagagt ttacagctag ctcagtccta ggtattatgc tagcTGGAAT TGTGAGCGGA 2101 TAACAATTGA AAGAAGAGAC TCATGGAAAT CAACGAAAAA CTGCTGCGTC AGATTATTGA 2161 GGACGTGTTA CGCGATACGT CGGCGGCCGC ACCAGGCGAA CGCCCATTTC AGTGTCGCAT 2221 CTGCATGCGT AATTTCTCTT CACCGAGCAA ACTTATTCGC CATACCCGTA CCCATACTGG 2281 CGAGAAACCC TTTCAGTGTC GTATCTGTAT GCGAAACTTC TCCGACGGGA GCAACCTGGC 2341 TCGGCACTTG CGTACACATA CCGGTGAAAA GCCGTTTCAA TGCCGTATTT GCATGCGCAA 2401 TTTCAGTCGC GTGGATAACT TACCGAGACA CCTGAAAACG CACTACCCGT ATGATGTTCC 2461 TGATTATGCG TAAtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag 2521 agagagaata taaaaagcca gattattaat ccggcttttt tattatttta ctagtagcgg 2581 ccgctgcag
//`
sequences.seq_pSB1A3GB3_backbone = {}; sequences.seq_pSB1A3GB3_backbone.varName = "seq_pSB1A3GB3_backbone"; sequences.seq_pSB1A3GB3_backbone.name = "pSB1A3-GB3 backbone"; sequences.seq_pSB1A3GB3_backbone.seqType = "both"; sequences.seq_pSB1A3GB3_backbone.data = `LOCUS pSB1A3-GB3_backbone 5268 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
primer_bind 4048..4066 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter 632..662 /label="lac promoter" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 terminator 2004..2061 /label="his operon terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind 3638..3657 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac RBS 704..715 /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 CDS complement(3015..3875) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 terminator 1444..1515 /label="rrnB T1 terminator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(2336..2355) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac source 1..5268 /label="source" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 protein_bind 4452..4485 /label="loxP" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 rep_origin complement(2256..2844) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(2085..2102) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 promoter complement(3876..3980) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 protein_bind 670..686 /label="lac operator" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2002..4115 /label="pSB1A3 Region" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e CDS 722..1399 /label="mRFP1" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 protein_bind 596..617 /label="CAP binding site" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 3156..3156 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 1531..1558 /label="T7Te terminator" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 4070..4113 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer complement(4088..4115) /label="BBa_Prefix & Promoter SDM Rev" /note="sequence: cagaaatcatccttagcgaaagctaagg" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer 4116..4135 /label="BBa_Prefix & Promoter SDM Fwd" /note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 2002..2019 /label="pSB1A3 fwd SapI" /note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(4093..4115) /label="BsaI pSB1A3 Rev" /note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer complement(4093..4115) /label="pSB1A3 SapI rev" /note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer 2002..2019 /label="BsaI pSB1A3 Fwd" /note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(4093..4115) /label="Linear pSB1A3 BsmBI Rev" /note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer 2002..2023 /label="Linear pSB1A3 BsmBI Fwd" /note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 2002..2019 /label="Linear pSB1A3 BsmBI Fwd" /note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2
ORIGIN
1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc 61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag 121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc 181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc 241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt 301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg 361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc 421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag 481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta 721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc 1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg 1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg 1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt 1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt 1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac 1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt 1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct 2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac 2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca 2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat 3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct 3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa 4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg 4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta 4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt 4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga 4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag 4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt 4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa 4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac 4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca 4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt 4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt 4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc 4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt 4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac 4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag 4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg 5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt 5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg 5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg 5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
//`
sequences.seq_pSB1C3_Forward_Primer = {}; sequences.seq_pSB1C3_Forward_Primer.varName = "seq_pSB1C3_Forward_Primer"; sequences.seq_pSB1C3_Forward_Primer.name = "pSB1C3 Forward Primer"; sequences.seq_pSB1C3_Forward_Primer.seqType = "linear"; sequences.seq_pSB1C3_Forward_Primer.data = `LOCUS pSB1C3_Forward_Primer 41 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TATGATCGTC TCAcaaagtc agaacagatg tttgccgatg c
//`
sequences.seq_pSB1C3_Reverse_Primer = {}; sequences.seq_pSB1C3_Reverse_Primer.varName = "seq_pSB1C3_Reverse_Primer"; sequences.seq_pSB1C3_Reverse_Primer.name = "pSB1C3 Reverse Primer"; sequences.seq_pSB1C3_Reverse_Primer.seqType = "linear"; sequences.seq_pSB1C3_Reverse_Primer.data = `LOCUS pSB1C3_Reverse_Primer 45 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACTGATCGTC TCTgttgtga agaaaaagtg aatgatgtag ccgtc
//`
sequences.seq_pSB1T3_Backbone = {}; sequences.seq_pSB1T3_Backbone.varName = "seq_pSB1T3_Backbone"; sequences.seq_pSB1T3_Backbone.name = "pSB1T3 Backbone"; sequences.seq_pSB1T3_Backbone.seqType = "both"; sequences.seq_pSB1T3_Backbone.data = `LOCUS pSB1T3_Backbone 2498 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 2389..2407 /label="pBRforEco" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind complement(120..137) /label="L4440" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 18..38 /label="BioBrick suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 2456..2477 /label="BioBrick prefix" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 terminator 39..96 /label="his operon terminator" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 terminator 2411..2454 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind complement(371..390) /label="pBR322ori-F" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 rep_origin complement(291..879) /label="ori" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS 1145..2329 /label="TetR" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86
ORIGIN
1 GGACTAGGTC TCATGCCtac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg 61 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc 121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg 301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 1021 agtatatatg agtaaacttg gtctgacagc tcgagattct catgtttgac agcttatcat 1081 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg 1141 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg 1201 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag 1261 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc 1321 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct 1381 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta 1441 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat 1501 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt 1561 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca 1621 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct 1681 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt 1741 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt 1801 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg 1861 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc 1921 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat 1981 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg 2041 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt 2101 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct 2161 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc 2221 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt 2281 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aactcgagtg 2341 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 2401 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctgaatt 2461 cgcggccgct tctagaggCG TTAGAGACCT AGAGCATT
//`
sequences.seq_pUCSTS_FWD_BspMI = {}; sequences.seq_pUCSTS_FWD_BspMI.varName = "seq_pUCSTS_FWD_BspMI"; sequences.seq_pUCSTS_FWD_BspMI.name = "pUC-STS FWD BspMI"; sequences.seq_pUCSTS_FWD_BspMI.seqType = "linear"; sequences.seq_pUCSTS_FWD_BspMI.data = `LOCUS pUC-STS_FWD_BspMI 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACAGTGGGTC TCCTAGTatg gtgtctgtga gtgag
//`
sequences.seq_pUCSTS_REV_BspMI = {}; sequences.seq_pUCSTS_REV_BspMI.varName = "seq_pUCSTS_REV_BspMI"; sequences.seq_pUCSTS_REV_BspMI.name = "pUC-STS REV BspMI"; sequences.seq_pUCSTS_REV_BspMI.seqType = "linear"; sequences.seq_pUCSTS_REV_BspMI.data = `LOCUS pUC-STS_REV_BspMI 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACAGTCGGTC TCCacaatta tatggccatg ctgcg
//`
sequences.seq_pUCSTS = {}; sequences.seq_pUCSTS.varName = "seq_pUCSTS"; sequences.seq_pUCSTS.name = "pUC-STS"; sequences.seq_pUCSTS.seqType = "both"; sequences.seq_pUCSTS.data = `LOCUS pUC-STS 3622 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_35949_44214" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
CDS 1632..2492 /label="AmpR" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 misc_feature 1154..1156 /label="Threonine inside BsaI site" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind complement(1262..1281) /label="pRS-marker" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind 3152..3171 /label="pBR322ori-F" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 source 23..1192 /label="STS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 rep_origin 2663..3251 /label="ori" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 promoter 1527..1631 /label="AmpR promoter" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter 3575..3605 /label="lac promoter" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(1850..1869) /label="Amp-R" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer_bind complement(1441..1459) /label="pBRforEco" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e protein_bind 3539..3560 /label="CAP binding site" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_feature 794..796 /label="Leu Codon inside BsaI Site " /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 primer_bind 1381..1403 /label="pGEX 3'" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 primer_bind 3405..3422 /label="L4440" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(1173..1192) /label="STS Reverse BspMI" /note="sequence: ACTCCTACCTGCTTCaACAAttatatggccatgctgcgga" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer complement(1193..1210) /label="stsreverseprimer" /note="sequence: aaataccgcagcggccgc" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer 23..44 /label="STS Forward BspMI" /note="sequence: ACTGCTACCTGCTCCcTAGTatggtgtctgtgagtgagatcc" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 primer 1..22 /label="forward primer" /note="sequence: ctagaagaaggaggattacaaa" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 ctagaagaag gaggattaca aaatggtgtc tgtgagtgag atccgcaaag ttcaaagggc 61 agaaggccct gcaactgtat tggcgatagg cacagcaaat ccaccaaatt gtattgatca 121 gagcacatat gctgattatt attttagagt aactaacagt gaacacatga ctgatctcaa 181 gaagaagttt cagcgcattt gtgagagaac acaaatcaag aacagacata tgtacttaac 241 agaagagata ctgaaagaga atcctaacat gtgcgcatac aaagcaccgt cgttggatgc 301 aagggaagac atgatgatca gggaggtacc aagggttgga aaagaggctg caaccaaggc 361 catcaaggaa tggggtcagc caatgtctaa gatcacacat ttgatcttct gcaccaccag 421 cggtgttgca ttgcctggcg ttgattacga actcatcgta ctcttaggac tcgacccatc 481 cgtcaagagg tacatgatgt accaccaagg ctgcttcgcc ggtggcactg tccttcgttt 541 ggctaaggac ttggctgaaa acaacaagga tgctcgtgtg cttatcgttt gttctgagaa 601 taccgcagtc actttccgtg gtcctagtga gacagacatg gatagtcttg tagggcaagc 661 cttgtttgct gatggagctg ctgcgattat cattggttct gatcctgtgc cagaggttga 721 aaagcctatc tttgaaattg tttcgactga tcaaaaactt gtccctaaca gccatggagc 781 catcggtggt ctccttcgtg aagttgggct tacattctat cttaataaga gtgttcctga 841 tattatttca caaaacatca atgatgcgct cagtaaagct tttgatccat tgggtatatc 901 tgattataac tcaatatttt ggattgcaca ccctggtgga cgtgcaattt tggatcaggt 961 tgaacagaaa gtgaacttga aaccagagaa gatgaatgcc actagagacg tgcttagcaa 1021 ttacggtaac atgtcaagtg cgtgtgtgtt cttcatcatg gatttaatga ggaagaagtc 1081 ccttgaagaa ggacttaaaa ctaccggtga aggacttgat tggggcgtac tttttggctt 1141 tggtcctggt ctcactattg aaactgttgt tctccgcagc atggccatat aagcggccgc 1201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca 1261 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg 1321 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct 1381 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg 1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt 1501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 1561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 1681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 1741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 1801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 1861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 1921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 1981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 2041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 2101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 2161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 2521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 2581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 3301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 3361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 3421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 3481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 3541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 3601 tgttgtgtgg aattgtgagc gt
//`
sequences.seq_Resveratrol_2x_Assembly = {}; sequences.seq_Resveratrol_2x_Assembly.varName = "seq_Resveratrol_2x_Assembly"; sequences.seq_Resveratrol_2x_Assembly.name = "Resveratrol 2x Assembly"; sequences.seq_Resveratrol_2x_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Assembly.data = `LOCUS Resveratrol_2x_Assembly 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2436..2444 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2425..2433 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2414..2422 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 HIV-RT Binding Site join(2445..2448,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2403..2411 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAA
//`
sequences.seq_Resveratrol_2x_Comp_Assembly = {}; sequences.seq_Resveratrol_2x_Comp_Assembly.varName = "seq_Resveratrol_2x_Comp_Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.name = "Resveratrol 2x Comp Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Comp_Assembly.data = `LOCUS Resveratrol_2x_Comp_Ass 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 HIV-RT Binding Site join(2445..2448,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2436..2444) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2425..2433) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site complement(2414..2422) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site complement(2403..2411) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2392..2400) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC 2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAA
//`
sequences.seq_Resveratrol_2x_Full_Part = {}; sequences.seq_Resveratrol_2x_Full_Part.varName = "seq_Resveratrol_2x_Full_Part"; sequences.seq_Resveratrol_2x_Full_Part.name = "Resveratrol 2x Full Part"; sequences.seq_Resveratrol_2x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_2x_Full_Part.data = `LOCUS Resveratrol_2x_Full_Par 2747 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 2414..2422 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Promoter 2632..2666 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site 2445..2554 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 HIV-RT Binding Site join(2744..2747,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2735..2743) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 misc_binding 2668..2690 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2713..2721) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Scar 2624..2631 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Terminator 2555..2623 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2425..2433 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2436..2444 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site 2403..2411 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site complement(2724..2732) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2702..2710) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2691..2699) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac gBlock 2501..2625 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC 2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG 2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga 2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT 2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA 2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAA
//`
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.name = "Resveratrol 2x r_oligo Complement Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_C 111 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature complement(42..50) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(53..61) /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(64..72) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(75..83) /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(86..94) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG 61 CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAACG AGACCAACCT G
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.name = "Resveratrol 2x r_oligo Duplex 11-33 FWD 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 23 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCCTAGCTGG AATTGTGAGC GGA
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.name = "Resveratrol 2x r_oligo Duplex 75-94 REV 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTTCCACACA TCGCCCACGC
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.name = "Resveratrol 2x r_oligo Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_D 111 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 42..50 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 53..61 /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 64..72 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 75..83 /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 86..94 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(75..94) /label="Resveratrol 2x r_oligo Duplex 75-94 REV 1" /note="sequence: TTTCCACACATCGCCCACGC" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 11..33 /label="Resveratrol 2x r_oligo Duplex 11-33 FWD 1" /note="sequence: TCCTAGCTGGAATTGTGAGCGGA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC 61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAACG AGACCAACCT G
//`
sequences.seq_Resveratrol_4x_Assembly = {}; sequences.seq_Resveratrol_4x_Assembly.varName = "seq_Resveratrol_4x_Assembly"; sequences.seq_Resveratrol_4x_Assembly.name = "Resveratrol 4x Assembly"; sequences.seq_Resveratrol_4x_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Assembly.data = `LOCUS Resveratrol_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 2447..2455 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2469..2477 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2458..2466 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Zinc Finger Site 2403..2411 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site 2425..2433 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Zinc Finger Site 2436..2444 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2414..2422 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 HIV-RT Binding Site join(2489..2492,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2480..2488 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG 2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AA
//`
sequences.seq_Resveratrol_4x_Comp_Assembly = {}; sequences.seq_Resveratrol_4x_Comp_Assembly.varName = "seq_Resveratrol_4x_Comp_Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.name = "Resveratrol 4x Comp Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Comp_Assembly.data = `LOCUS Resveratrol_4x_Comp_Ass 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Zinc Finger Site complement(2469..2477) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2392..2400) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site complement(2458..2466) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site complement(2480..2488) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2414..2422) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site complement(2436..2444) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 HIV-RT Binding Site join(2489..2492,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2425..2433) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site complement(2403..2411) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(2447..2455) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC 2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT 2461 CCACACATCG CCCACGCATG CCGTCCTCAA AA
//`
sequences.seq_Resveratrol_4x_Full_Part = {}; sequences.seq_Resveratrol_4x_Full_Part.varName = "seq_Resveratrol_4x_Full_Part"; sequences.seq_Resveratrol_4x_Full_Part.name = "Resveratrol 4x Full Part"; sequences.seq_Resveratrol_4x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_4x_Full_Part.data = `LOCUS Resveratrol_4x_Full_Par 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site complement(2823..2831) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site complement(2768..2776) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2392..2400 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 Zinc Finger Site 2403..2411 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2480..2488 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 terminator 2265..2308 /label="bacterial terminator" /ApEinfo_revcolor=#ffef86 /ApEinfo_fwdcolor=#ffef86 Scar 2668..2675 /label="BioBrick Scar" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 primer_bind complement(280..297) /label="L4440" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Zinc Finger Site complement(2812..2820) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 2243..2261 /label="pBRforEco" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site 2414..2422 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1351..1351 /label="Point Mutation" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 Promoter 2333..2367 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2779..2787) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site 2436..2444 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer_bind complement(531..550) /label="pBR322ori-F" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac Biobricking 2311..2332 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc HIV-RT Binding Site 2489..2598 /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site 2458..2466 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 HIV-RT Binding Site join(2832..2835,1..106) /label="C* HTBS" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 Zinc Finger Site complement(2735..2743) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_binding 2712..2734 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Terminator 2599..2667 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2425..2433 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site 2469..2477 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Backbone 197..2310 /label="Mutated pSB1A3" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e Promoter 2676..2710 /label="J23100 Anderson Promoter" /ApEinfo_revcolor=#d59687 /ApEinfo_fwdcolor=#d59687 Zinc Finger Site complement(2790..2798) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 gBlock 2545..2669 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 promoter complement(2071..2175) /label="AmpR promoter" /ApEinfo_revcolor=#faac61 /ApEinfo_fwdcolor=#faac61 CDS complement(1210..2070) /label="AmpR" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2757..2765) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 Zinc Finger Site 2447..2455 /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 primer_bind 1833..1852 /label="Amp-R" /ApEinfo_revcolor=#b4abac /ApEinfo_fwdcolor=#b4abac misc_binding 2369..2391 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc gBlock 53..196 /label="gBlock B" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Zinc Finger Site complement(2801..2809) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 rep_origin complement(451..1039) /label="ori" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 Zinc Finger Site complement(2746..2754) /label="Zif268 Site" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Biobricking 176..196 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Terminator 107..175 /label="B0054" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC 61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc 121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta 181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc 241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg 301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca 481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc 1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt 1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac 1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC 2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC 2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG 2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT 2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC 2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact 2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA 2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC 2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC 2821 ATGCCGTCCT CAAAA
//`
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.name = "Resveratrol 4x r_oligo Complement Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_C 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature complement(42..50) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(53..61) /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(64..72) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(75..83) /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(86..94) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(97..105) /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature complement(108..116) /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature complement(119..127) /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 Zinc Finger Site complement(130..138) /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG 61 CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT CCACACATCG 121 CCCACGCATG CCGTCCTCAA AACGAGACCA ACCTG
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.name = "Resveratrol 4x r_oligo Duplex 109-128 REV 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGCCCACGC ATTTTCCACA
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.name = "Resveratrol 4x r_oligo Duplex 39-58 FWD 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATTGAGGACG GCATGCGTGG
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.name = "Resveratrol 4x r_oligo Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_D 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc Zinc Finger Site 42..50 /label="ZFc Site" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 53..61 /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 64..72 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 75..83 /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 86..94 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 97..105 /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 108..116 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 119..127 /label="Zif268" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 130..138 /label="PBSII Site" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer 39..58 /label="Resveratrol 4x r_oligo Duplex 39-58 FWD 1" /note="sequence: ATTGAGGACGGCATGCGTGG" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 primer complement(109..128) /label="Resveratrol 4x r_oligo Duplex 109-128 REV 1" /note="sequence: TCGCCCACGCATTTTCCACA" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC 61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG TGGAAAATGC 121 GTGGGCGATG TGTGGAAAAA AACGAGACCA ACCTG
//`
sequences.seq_SapI_FWD_Primer = {}; sequences.seq_SapI_FWD_Primer.varName = "seq_SapI_FWD_Primer"; sequences.seq_SapI_FWD_Primer.name = "SapI FWD Primer"; sequences.seq_SapI_FWD_Primer.seqType = "linear"; sequences.seq_SapI_FWD_Primer.data = `LOCUS SapI_FWD_Primer 32 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 AAAGCTCTTC AGGAagtccg gcaaaaaagg gc
//`
sequences.seq_SapI_Rev_Primer = {}; sequences.seq_SapI_Rev_Primer.varName = "seq_SapI_Rev_Primer"; sequences.seq_SapI_Rev_Primer.name = "SapI Rev Primer"; sequences.seq_SapI_Rev_Primer.seqType = "linear"; sequences.seq_SapI_Rev_Primer.data = `LOCUS SapI_Rev_Primer 37 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CCCGCTCTTC AGTGcagaaa tcatccttag cgaaagc
//`
sequences.seq_STS_intro_gblock = {}; sequences.seq_STS_intro_gblock.varName = "seq_STS_intro_gblock"; sequences.seq_STS_intro_gblock.name = "STS intro gblock"; sequences.seq_STS_intro_gblock.seqType = "linear"; sequences.seq_STS_intro_gblock.data = `LOCUS STS_intro_gblock 474 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 40..74 /label="BBa_J23111" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 123..431 /label="PBSII" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 99..112 /label="BBa_J61101" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 Biobricking 18..39 /label="BioBrick Prefix " /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_binding 75..98 /label="LacO" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc misc_feature 432..461 /label="Spacer" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer 18..41 /label="forward Prefix-Promoter-PBS2-sapI primer" /note="sequence: AAAGCTCTTCACACgaattcgcggccgcttctagagtt" /ApEinfo_revcolor=#85dae9 /ApEinfo_fwdcolor=#85dae9 primer complement(439..461) /label="reverse Prefix-Promoter-PBS2-sapI primer" /note="sequence: AAAGCTCTTCAACTactacctccacttccacctccac" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCATCGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta 61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGACAGGAC CCtaaataca 121 tgATGCATCA CCATCACCAT CATCCAGGCG AGAAACCGTA CGCCTGCCCC GAATGCGGTA 181 AATCATTTAG TCAGCGGGCG AACTTACGCG CACACCAGCG AACCCATACC GGAGAAAAAC 241 CGTATAAATG TCCGGAATGT GGTAAAAGCT TTAGCCGTTC GGATCATCTG ACGACTCACC 301 AACGCACACA CACCGGGGAA AAGCCGTATA AATGCCCTGA GTGTGGTAAG TCGTTCTCCC 361 GTTCTGACGT TCTGGTGCGC CATCAGCGTA CGCATACTGG TGGCGGGAGC GGCGGCGGAA 421 GTGGTGGCTC Cggtggaagt ggaggtggaa gtggaggtag tAGAGACCTC CTTT
//`
sequences.seq_STS_outro_gblock = {}; sequences.seq_STS_outro_gblock.varName = "seq_STS_outro_gblock"; sequences.seq_STS_outro_gblock.name = "STS outro gblock"; sequences.seq_STS_outro_gblock.seqType = "linear"; sequences.seq_STS_outro_gblock.data = `LOCUS STS_outro_gblock 129 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
stem_loop 27..78 /label="T0 terminator" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 Biobricking 79..99 /label="BioBrick Suffix" /ApEinfo_revcolor=#84b0dc /ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 gtcaagctgc catgacgagG GTCTCAttgt tcagaacgct cggtcttgca caccgggcgt 61 tttttctttg tgagtccata ctagtagcgg ccgctgcagT GCCAGAGACC tccaagagag 121 tgaagggat
//`
sequences.seq_ZFc_codon_optimized = {}; sequences.seq_ZFc_codon_optimized.varName = "seq_ZFc_codon_optimized"; sequences.seq_ZFc_codon_optimized.name = "ZFc (codon optimized)"; sequences.seq_ZFc_codon_optimized.seqType = "linear"; sequences.seq_ZFc_codon_optimized.data = `LOCUS ZFc_(codon_optimized) 331 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 18..311 /label="ZFc with linker" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd
ORIGIN
1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA 61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG 121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA 181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA 241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC 301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T
//`