Template:Virginia/scripts/seqDataJS
/*This file is used to store the text from the .gb files in a form that's readable by other scripts*/ var sequences = {}; sequences.seq_2x_Res_6x_CoA = {}; sequences.seq_2x_Res_6x_CoA.varName = "seq_2x_Res_6x_CoA"; sequences.seq_2x_Res_6x_CoA.name = "(2x Res 6x CoA)"; sequences.seq_2x_Res_6x_CoA.seqType = "both"; sequences.seq_2x_Res_6x_CoA.data = `LOCUS (2x_Res,_6x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_binding 2668..2690
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3089..3097
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3078..3086
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3034..3042
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2923..2930
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_binding 2967..2989
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 2501..2625
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2702..2710)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3100..3108
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2744..2853
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3410..3418)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 2931..2965
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(3421..3429)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
HIV-RT Binding Site join(3518..3521,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3465..3473)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(3443..3451)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3509..3517)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3122..3130
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3377..3385)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
gBlock 3187..3311
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3023..3031
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3045..3053
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3487..3495)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 3310..3317
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2691..2699)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 3001..3009
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3498..3506)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3012..3020
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(3454..3462)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 3318..3352
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Terminator 3241..3309
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(3399..3407)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Terminator 2555..2623
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(3432..3440)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(3388..3396)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 3056..3064
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3067..3075
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Scar 2624..2631
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 2854..2922
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3111..3119
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2990..2998
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Promoter 2632..2666
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2724..2732)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2735..2743)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
HIV-RT Binding Site 2445..2554
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
HIV-RT Binding Site 3131..3240
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(3476..3484)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 3354..3376
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2713..2721)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
gBlock 2800..2924
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAAAAA ACGTGGCGCC
2761 CGAACAGGGA CGGATAGCTC AGTCGGTAGA GCATCAGACT TTTAATCTGA GGGTCCAGGG
2821 TTCAAGTCCC TGTTCGGGCG CCACGTTTTT TTTattagca gaaagtcaaa agcctccgac
2881 cggaggcttt tgactaaaac ttcccttggg gttatcattg ggtactagag TTGACGGCTA
2941 GCTCAGTCCT AGGTACAGTG CTAGCTGGAA TTGTGAGCGG ATAACAATTG AGGACGGCAT
3001 GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC
3061 TGGGATGTCG ATGCCATGCG GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
3121 TGCGGCTGGG AAAAAAAACG TGGCGCCCGA ACAGGGACGG ATAGCTCAGT CGGTAGAGCA
3181 TCAGACTTTT AATCTGAGGG TCCAGGGTTC AAGTCCCTGT TCGGGCGCCA CGTTTTTTTT
3241 attagcagaa agtcaaaagc ctccgaccgg aggcttttga ctaaaacttc ccttggggtt
3301 atcattgggt actagagTTG ACGGCTAGCT CAGTCCTAGG TACAGTGCTA GCTGGAATTG
3361 TGAGCGGATA ACAATTCCCA GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT
3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
//`
sequences.seq_4x_Res_4x_CoA = {}; sequences.seq_4x_Res_4x_CoA.varName = "seq_4x_Res_4x_CoA"; sequences.seq_4x_Res_4x_CoA.name = "(4x Res 4x CoA)"; sequences.seq_4x_Res_4x_CoA.seqType = "both"; sequences.seq_4x_Res_4x_CoA.data = `LOCUS (4x_Res,_4x_CoA) 3521 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site 3089..3097
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2779..2787)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3421..3429)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3122..3130
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 2599..2667
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 2447..2455
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3133..3141
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Terminator 2942..3010
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 3155..3163
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3454..3462)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2757..2765)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3465..3473)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 3055..3077
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_binding 2712..2734
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2668..2675
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 3111..3119
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(3498..3506)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
HIV-RT Binding Site join(3518..3521,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2469..2477
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Scar 3354..3361
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site complement(2768..2776)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Promoter 3362..3396
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2735..2743)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 3144..3152
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2812..2820)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Promoter 3019..3053
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(3509..3517)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
HIV-RT Binding Site 3175..3284
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2790..2798)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 3078..3086
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Terminator 3285..3353
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(3432..3440)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 3398..3420
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Scar 3011..3018
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
gBlock 3231..3355
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(3443..3451)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site complement(2823..2831)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site complement(3476..3484)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
gBlock 2545..2669
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2480..2488
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2801..2809)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Promoter 2676..2710
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(3487..3495)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 3100..3108
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2489..2598
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
gBlock 2888..3012
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2832..2941
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2746..2754)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2458..2466
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 3166..3174
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
2821 ATGCCGTCCT CAAAAAAAAC GTGGCGCCCG AACAGGGACG GATAGCTCAG TCGGTAGAGC
2881 ATCAGACTTT TAATCTGAGG GTCCAGGGTT CAAGTCCCTG TTCGGGCGCC ACGTTTTTTT
2941 Tattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt
3001 tatcattggg tactagagTT GACGGCTAGC TCAGTCCTAG GTACAGTGCT AGCTGGAATT
3061 GTGAGCGGAT AACAATTGAG GACGGCATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA
3121 TGCGGCTGGG ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGAAAAAA
3181 AACGTGGCGC CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG
3241 AGGGTCCAGG GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa
3301 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga
3361 gTTGACGGCT AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT
3421 CCCAGCCGCA TGGCATCGAC ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT
3481 CGACATCCCA GCCGCATGGC ATCGACATGC CGTCCTCAAA A
//`
sequences.seq_Addgene_109383_pSB1C3Lux = {}; sequences.seq_Addgene_109383_pSB1C3Lux.varName = "seq_Addgene_109383_pSB1C3Lux"; sequences.seq_Addgene_109383_pSB1C3Lux.name = "(Addgene 109383) pSB1C3-Lux"; sequences.seq_Addgene_109383_pSB1C3Lux.seqType = "both"; sequences.seq_Addgene_109383_pSB1C3Lux.data = `LOCUS (Addgene_#109383)_pSB1C 9707 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_109383_206254" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
terminator 1..58
/label="his operon terminator"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
rep_origin complement(253..841)
/label="ori"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 1024..1118
/label="lambda t0 terminator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
promoter 2955..3239
/label="araBAD promoter"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 1982..2025
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2028..2049
/label="BioBrick prefix"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
CDS complement(1139..1798)
/label="CmR"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer_bind complement(82..99)
/label="L4440"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature join(9687..9707,1..2049)
/label="pSB1C3 Backbone Region"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
source 1..9707
/label="source"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 1960..1978
/label="pBRforEco"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
CDS complement(2050..2928)
/label="araC"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer_bind complement(333..352)
/label="pBR322ori-F"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer_bind 3162..3181
/label="pBAD Forward"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 9687..9707
/label="BioBrick suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 9647..9650
/label="Overhang for end of pduD part"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1713..1732
/label="CAT-R"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer complement(2062..2093)
/label="pSB1C3 Reverse Primer"
/note="sequence: ACTGATCGTCTCTgttgtgaagaaaaagtgaatgatgtagccgtc"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer 9647..9670
/label="pSB1C3 Forward Primer"
/note="sequence: TATGATCGTCTCAgtcagaacagatgtttgccgatgc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
ORIGIN
1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
2041 cttctagagt tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg
2101 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc
2161 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag
2221 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg
2281 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat
2341 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt
2401 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc
2461 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat
2521 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt
2581 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta
2641 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc
2701 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac
2761 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc
2821 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc
2881 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact
2941 cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt
3001 cttttactgg ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta
3061 acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag
3121 aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc
3181 cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg tttctccata
3241 cccgtttttt tgggctagca gggaaaataa tgaataaatg tattccaatg ataattaatg
3301 gaatgattca agattttgat aattatgcat ataaagaagt taaactaaat aatgataata
3361 gagtaaaatt atctgtcatt actgaaagtt cagtttcaaa aacattaaat atcaaagata
3421 gaattaatct aaatttaaat cagattgtga attttttata taccgttggt caacgatgga
3481 aaagtgaaga atataatcgg cgacgaacct atatccgtga gttaaaaaca tatcttggtt
3541 attctgatga aatggcaaga ttagaagcga attggattgc aatgttattg tgctctaaaa
3601 gtgctttgta tgacattgtt aattatgatt tgggctctat acacgtatta gatgaatggc
3661 ttccacgtgg tgattgctat gttaaagcac aaccgaaagg tgtttctgtt cacttgttag
3721 ctggtaatgt tccattatca ggagtgacat ctattttgcg tgctatttta acaaaaaatg
3781 agtgcattat taaaacttcg tcttcagatc cttttactgc aaacgcttta gtttccagtt
3841 ttattgatgt taatgcagac catccaataa ccaaatcaat gtctgttatg tattggccgc
3901 atgatgaaga tatgactcta tctcaaagaa taatgaatca tgccgacgtg gttattgctt
3961 ggggtggaga cgaggcgatt aaatgggcgg taaaatattc accaccgcat gtcgatattc
4021 tgaaatttgg accaaagaaa agcttaagta ttattgaagc tcctaaagat atagaagccg
4081 cagcaatggg ggttgctcat gatatttgtt tctatgacca gcaagcctgc ttctctactc
4141 aagacgttta ttatatagga gataatttac ctttattttt aaatgaactt gaaaaacagc
4201 tagatcgata cgcgaaaatt ttaccaaaag gttcaaatag ttttgatgaa aaagcggcgt
4261 ttactcttac tgaaaaagaa agtctatttg ctggatatga agtgagaaag ggagataagc
4321 aagcttggtt aatagtcgta tcacctacaa atagctttgg aaatcaaccg ctatcacgaa
4381 gtgtgtatgt tcatcaagta tctgatatta aagagataat tccttttgtt aataaaaata
4441 gaacacaaac tgtttctatt tatccttggg aagcgtcatt aaaatatcga gataaattag
4501 caagaagtgg agttgaaaga attgttgaat caggcatgaa taatattttc agagttggag
4561 gggctcatga ttcattatct cctctccagt acctagttag gtttgtatcg catgagagac
4621 catttaatta tacgacaaaa gatgttgcgg ttgaaatcga acaaacacgt tacttagagg
4681 aagataagtt tttagttttt gtcccatagt taaaggaaat tatatgaaag atgaaagtgc
4741 tttttttacg attgatcaca tcatcaagct tgataatggt cagtctatcc gagtttggga
4801 aacactccct aaaaagaacg taccagagaa aaaacataca atacttattg cttcgggttt
4861 tgctagaaga atggatcatt ttgcaggtct tgctgagtat ttatctacta acggttttca
4921 tgtcattcgc tacgattctt tgcatcatgt tggattaagc agtggatgta taaatgaatt
4981 tacgatgtcg attggaaaaa atagcctgct tacagtcgta gattggctta cagatcatgg
5041 tgtcgaacga atagggctga ttgctgctag tttgtcagcg agaatcgcct atgaggtagt
5101 aaataaaatt aaattatcat ttttaattac ggccgtaggt gtcgttaatc ttagagatac
5161 attagaaaaa gcattggagt atgactattt gcaattacct atttcagagt taccagaaga
5221 tcttgacttt gaaggtcata atttaggatc ggaggtcttt gttacagatt gctttaaaca
5281 taattgggac acgttagact cgacacttaa tagtgttaaa ggattagcga ttccatttat
5341 tgcttttact gcaaacgatg atagttgggt aaagcaaagt gaagttatag agctcattga
5401 tagcattgaa tctagtaatt gtaagctcta ttcgctaatt ggaagttcac atgatcttgg
5461 ggaaaatttg gttgtattaa gaaattttta tcaatcagtg acgaaggcag ccttagcatt
5521 agatgatggt ttattggatt tagagataga cattattgaa cctcgatttg aggacgttac
5581 aagtattact gttaaggagc gtagattaaa aaatgaaatt gaaaatgaat tattagaatt
5641 ggcttaaata aacagaatca ccaaaaagga atagagtatg aagtttggaa atatttgttt
5701 ttcgtatcaa ccaccaggtg aaactcataa gcaagtaatg gatcgctttg ttcggcttgg
5761 tatcgcctca gaagaggtag ggtttgatac atattggacc ttagaacatc attttacaga
5821 gtttggtctt acgggaaatt tatttgttgc tgcggctaac ctgttaggaa gaactaaaac
5881 attaaatgtt ggcactatgg gggttgttat tccgacagca cacccagttc gacagttaga
5941 agacgtttta ttattagatc aaatgtcgaa aggtcgtttt aattttggaa ccgttcgagg
6001 gctataccat aaagattttc gagtatttgg tgttgatatg gaagagtctc gagcaattac
6061 tcaaaatttc taccagatga taatggaaag cttacagaca ggaaccatta gctctgatag
6121 tgattacatt caatttccta aggttgatgt atatcccaaa gtgtactcaa aaaatgtacc
6181 aacctgtatg actgctgagt ccgcaagtac gacagaatgg ctagcaatac aagggctacc
6241 aatggttctt agttggatta ttggtactaa tgaaaaaaaa gcacagatgg aactctataa
6301 tgaaattgcg acagaatatg gtcatgatat atctaaaata gatcattgta tgacttatat
6361 ttgttctgtt gatgatgatg cacaaaaggc gcaagatgtt tgtcgggagt ttctgaaaaa
6421 ttggtatgac tcatatgtaa atgcgaccaa tatctttaat gatagcaatc aaactcgtgg
6481 ttatgattat cataaaggtc aatggcgtga ttttgtttta caaggacata caaacaccaa
6541 tcgacgtgtt gattatagca atggtattaa ccctgtaggc actcctgagc agtgtattga
6601 aatcattcaa cgtgatattg atgcaacggg tattacaaac attacatgcg gatttgaagc
6661 taatggaact gaagatgaaa taattgcttc catgcgacgc tttatgacac aagtcgctcc
6721 tttcttaaaa gaacctaaat aaattactta tttgatacta gagataataa ggaacaagtt
6781 atgaaatttg gattattttt tctaaacttt cagaaagatg gaataacatc tgaagaaacg
6841 ttggataata tggtaaagac tgtcacgtta attgattcaa ctaaatatca ttttaatact
6901 gcctttgtta atgaacatca cttttcaaaa aatggtattg ttggagcacc tattaccgca
6961 gctggttttt tattagggtt aacaaataaa ttacatattg gttcattaaa tcaagtaatt
7021 accacccatc accctgtacg tgtagcagaa gaagccagtt tattagatca aatgtcagag
7081 ggacgcttca ttcttggttt tagtgactgc gaaagtgatt tcgaaatgga attttttaga
7141 cgtcatatct catcaaggca acaacaattt gaagcatgct atgaaataat taatgacgca
7201 ttaactacag gttattgtca tccccaaaac gacttttatg attttccaaa ggtttcaatt
7261 aatccacact gttacagtga gaatggacct aagcaatatg tatccgctac atcaaaagaa
7321 gtcgtcatgt gggcagcgaa aaaggcactg cctttaacat ttaagtggga ggataattta
7381 gaaaccaaag aacgctatgc aattctatat aataaaacag cacaacaata tggtattgat
7441 atttcggatg ttgatcatca attaactgta attgcgaact taaatgctga tagaagtacg
7501 gctcaagaag aagtgagaga atacttaaaa gactatatca ctgaaactta ccctcaaatg
7561 gacagagatg aaaaaattaa ctgcattatt gaagagaatg cagttgggtc tcatgatgac
7621 tattatgaat cgacaaaatt agcagtggaa aaaacagggt ctaaaaatat tttattatcc
7681 tttgaatcaa tgtccgatat taaagatgta aaagatatta ttgatatgtt gaaccaaaaa
7741 atcgaaatga atttaccata ataaaattaa aggcaatttc tatattagat tgccttttta
7801 aatttctgtt gatattaggt attactggag agggtatgac tgtccatact gaatataaaa
7861 gaaatcaaat cattgctagt tcagaaattg atgatcttat ctttatgacg aaaccacaag
7921 agtggtcatt tgaagagcaa aaagaaatac gggataaatt agttcgtgag gctttttatt
7981 ttcactacaa tagaaatgaa gaatatagaa attattgtat caatcagcat gtgagtgata
8041 atttacacac tattgatgaa atacccgtgt ttccaacatc tgtttttaaa tataagaaat
8101 tacatactgt cacagccgag gacattgaaa attggtatac aagtagtgga actcgtggag
8161 taaaaagtca tattgcacgt gatcgtctta gcattgaacg cttgcttggt tctgtcaact
8221 tcggaatgaa atacgttgga gattggtttg agcatcaaat ggaattgata aatttaggac
8281 cagatagatt caatacaaat aatatttggt ttaaatatgt catgagtttg gtcgagttac
8341 tttatccgac tgaatttaca gttgataatg acaaaataga ttttgaaaaa acagtaaaac
8401 atctatttag aattaagaat agtaaaaaag acatttgctt aattgggcca ccattttttg
8461 tgtatctttt gtgccaatat atgaaagaaa acaatattga atttaaagga ggagatagag
8521 tacatattat tactggtgga ggatggaaat ctaatcagaa tgactcttta gatcgtgctg
8581 attttaatca attattaatg gatactttcc aactcgacaa gattaatcaa attagagata
8641 cctttaatca agttgagctt aatacttgtt tttttgaaga tgaatttcaa agaaaacatg
8701 ttccaccgtg ggtatatgct cgggctcttg atcctgaaac cttgaaaccc gtagcagatg
8761 gtgagatcgg gttgttaagt tatatggacg cctcatcaac tgcttaccct gcttttattg
8821 ttactgatga tatcggtatt gtaaaagaaa ttagagaacc agatccttac ccaggggtaa
8881 ctgttgagat tgttcggcgc ttaaatacac gtgcgcaaaa aggatgcgcg ctctctatgg
8941 ctaatgtcat acaaaagaat atcaaggatt aagttatgat tgttgatggt agagtttcaa
9001 aaatagtatt agcgtcaata agaaataata tatataaggt atttattact gtaaattcac
9061 caataaagtt catcgctgga caatttgtaa tggtcacgat taatgggaaa aaatgccctt
9121 tttcaattgc gaattgcccg acaaaaaatt acgaaataga attgcatatt ggtagttcga
9181 atagagactg ctcattggat attatcgaat attttgtcga tgctcttgtt gaggaagtcg
9241 caattgagtt agatgctccc catggaaacg cttggttacg gtctgaaagt aataacccat
9301 tgctattaat tgcgggaggt acaggtttat catatataaa tagcattcta acaaattgct
9361 taaataggaa tatacctcaa gatatttatc tttactgggg agtaaaagac agttctcttt
9421 tgtatgaaga tgaagagtta ctaaacttat cactaaacaa caaaaacttt cattatattc
9481 ctgttattga agataaaagt gaagaatgga tagggagaaa aggcactgtt cttgatgctg
9541 tcatggaaga ttttactgat cttacttatt ttgatattta tgtttgtgga cccttcatga
9601 tggctaaaac agcaaaagaa aaattaattg aagagaaaaa agcaaagtca gaacagatgt
9661 ttgccgatgc ttttgcatac gtataatact agtagcggcc gctgcag
//`
sequences.seq_4CL_Intro_gblock = {}; sequences.seq_4CL_Intro_gblock.varName = "seq_4CL_Intro_gblock"; sequences.seq_4CL_Intro_gblock.name = "4CL Intro gblock"; sequences.seq_4CL_Intro_gblock.seqType = "linear"; sequences.seq_4CL_Intro_gblock.data = `LOCUS 4CL_Intro_gblock 480 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 437..466
/label="Spacer"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 40..73
/label="BBa_J23102"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 122..436
/label="ZIF268"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 98..111
/label="BBa_J61115"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Biobricking 18..39
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_binding 74..97
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 CGTCAGGCTC TTCACACgaa ttcgcggccg cttctagagt gacagctagc tcagtcctag
61 gtactgtgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGGGATA Ctaaatacat
121 gATGCACCAC CATCACCATC ATCCCGGTGA GAAACCATAT GCGTGCCCGG TGGAAAGCTG
181 TGATAGACGC TTTAGCCGTA GTGACGAACT GACCCGCCAC ATTCGAATAC ATACGGGGCA
241 AAAACCGTTT CAGTGCCGCA TTTGTATGCG TAACTTCTCA CGGAGTGATC ATTTAACCAC
301 TCATATTCGT ACTCATACAG GCGAAAAACC TTTTGCCTGC GACATCTGTG GTCGCAAATT
361 CGCACGTTCC GATGAGCGCA AGCGTCACAC GAAAATCCAT ACCGGTGGCG GGAGCGGCGG
421 CGGATCTGGT GGCTCGggtg gaagtggagg tggaagtgga ggtagtAGAA GAGCTGCAAG
//`
sequences.seq_4CL_Outro_gblock = {}; sequences.seq_4CL_Outro_gblock.varName = "seq_4CL_Outro_gblock"; sequences.seq_4CL_Outro_gblock.name = "4CL Outro gblock"; sequences.seq_4CL_Outro_gblock.seqType = "linear"; sequences.seq_4CL_Outro_gblock.data = `LOCUS 4CL_Outro_gblock 137 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
stem_loop 15..66
/label="T0 terminator"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Biobricking 67..87
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 TTCCGGGCTC TTCAttgttc agaacgctcg gtcttgcaca ccgggcgttt tttctttgtg
61 agtccatact agtagcggcc gctgcagGGA AGAAGAGCtc caagagagtg aagggatgtc
121 aagctgccat gAATTGA
//`
sequences.seq_ACC_intro_gblock = {}; sequences.seq_ACC_intro_gblock.varName = "seq_ACC_intro_gblock"; sequences.seq_ACC_intro_gblock.name = "ACC intro gblock"; sequences.seq_ACC_intro_gblock.seqType = "both"; sequences.seq_ACC_intro_gblock.data = `LOCUS ACC_intro_gblock 2034 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 99..112
/label="RBS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 40..74
/label="Promoter"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 113..1885
/label="ACCalpha"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1889..2017
/label="Terminator"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_binding 75..98
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 18..39
/label="Prefix"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
ORIGIN
1 AATGCTGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGAGGTGAC ATATGAGCGT
121 GGAAACCCGT AAAATCACTA AAGTTTTGGT CGCGAACCGG GGTGAAATCG CTATTCGCGT
181 TTTTCGTGCG GCCCGCGATG AGGGTATCGG CAGTGTTGCT GTCTACGCCG AGCCGGATGC
241 CGATGCGCCA TTCGTGAGCT ATGCGGACGA AGCCTTTGCC CTGGGCGGCC AGACATCGGC
301 CGAATCGTAT CTGGTAATCG ACAAAATCAT TGACGCCGCT CGTAAGAGCG GGGCTGATGC
361 AATCCATCCC GGATACGGCT TCCTTGCAGA AAACGCGGAC TTCGCCGAAG CTGTTATAAA
421 CGAAGGGCTA ATTTGGATTG GCCCGTCCCC AGAGAGCATC AGATCCTTAG GAGACAAAGT
481 AACAGCGCGG CATATTGCGG ATACCGCAAA GGCGCCGATG GCACCCGGGA CGAAAGAGCC
541 CGTGAAAGAT GCCGCCGAAG TCGTGGCTTT TGCAGAGGAG TTTGGGCTGC CGATCGCTAT
601 CAAAGCAGCC TTTGGTGGTG GTGGCCGTGG AATGAAGGTG GCCTACAAAA TGGAAGAAGT
661 CGCGGATCTG TTTGAGTCTG CGACCCGTGA AGCGACCGCC GCTTTCGGTC GAGGTGAGTG
721 CTTTGTGGAA CGGTATCTGG ACAAAGCGCG CCACGTGGAA GCGCAAGTCA TCGCAGATAA
781 ACATGGAAAC GTCGTAGTGG CGGGCACCCG CGACTGCTCG CTTCAGCGCC GTTTTCAAAA
841 ACTCGTGGAA GAAGCGCCGG CACCGTTTCT CACTGATGAC CAAAGAGAGC GTTTACACTC
901 AAGTGCAAAG GCGATTTGTA AAGAAGCTGG CTATTATGGT GCTGGCACTG TTGAATACTT
961 GGTTGGTTCT GATGGACTCA TCTCGTTCCT GGAAGTGAAT ACCCGTCTGC AAGTCGAACA
1021 CCCTGTGACG GAAGAAACGA CAGGTATCGA CCTGGTTCGC GAAATGTTTC GTATTGCGGA
1081 AGGTCATGAA CTGTCTATTA AAGAAGATCC AGCACCCCGC GGCCACGCCT TCGAGTTCCG
1141 TATTAACGGC GAGGATGCCG GCTCAAACTT CATGCCGGCT CCTGGAAAGA TTACTAGCTA
1201 CCGAGAACCA CAGGGGCCTG GCGTTCGTAT GGACTCCGGC GTCGTGGAGG GGAGCGAAAT
1261 ATCTGGTCAG TTTGACAGTA TGTTGGCAAA GCTTATTGTC TGGGGCGATA CCCGCGAACA
1321 GGCCTTACAG CGCAGCCGTC GCGCCCTGGC GGAATATGTA GTGGAGGGTA TGCCGACCGT
1381 AATCCCGTTC CACCAGCATA TTGTGGAAAA TCCGGCCTTT GTTGGGAATG ATGAAGGTTT
1441 TGAAATTTAC ACCAAATGGA TAGAGGAAGT ATGGGACAAT CCTATTGCAC CTTATGTGGA
1501 TGCGTCCGAA CTGGATGAGG ATGAAGATAA AACGCCAGCG CAGAAAGTTG TTGTAGAAAT
1561 TAATGGCCGC CGCGTTGAGG TGGCGCTGCC GGGCGATCTG GCACTGGGCG GGACAGCGGG
1621 ACCGAAAAAA AAGGCTAAAA AACGGAGGGC GGGCGGTGCG AAAGCAGGCG TCAGTGGGGA
1681 TGCGGTAGCG GCACCGATGC AGGGTACGGT GATTAAAGTA AATGTCGAGG AAGGCGCAGA
1741 GGTGAACGAA GGTGATACCG TGGTTGTACT TGAAGCAATG AAGATGGAAA ACCCGGTGAA
1801 AGCCCATAAA TCAGGCACGG TGACGGGTTT GACCGTTGCC GCGGGTGAAG GCGTCAATAA
1861 AGGTGTGGTT CTGTTAGAGA TTAAATAAcc aggcatcaaa taaaacgaaa ggctcagtcg
1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
1981 actggctcac cttcgggtgg gcctttctgc gtttatatag tAGAGACCAG GTGC
//`
sequences.seq_ACC_outro_gblock = {}; sequences.seq_ACC_outro_gblock.varName = "seq_ACC_outro_gblock"; sequences.seq_ACC_outro_gblock.name = "ACC outro gblock"; sequences.seq_ACC_outro_gblock.seqType = "both"; sequences.seq_ACC_outro_gblock.data = `LOCUS ACC_outro_gblock 2216 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 2179..2199
/label="Suffix"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 388..417
/label="Spacer"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 18..52
/label="Promoter"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 2050..2178
/label="Terminator"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 418..2049
/label="ACCbeta"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 77..90
/label="RBS"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 94..387
/label="ZFA"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 53..76
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 CCAGCCGGTC TCATAGTttg acggctagct cagtcctagg tatagtgcta gcTGGAATTG
61 TGAGCGGATA ACAATTGAAA GAGGTGACAT ATGACATCTG CAGCGGCCCC GGGGGAACGG
121 CCATTCCAGT GTCGGATTTG CATGAGAAAC TTTAGCGATT CACCGACCCT GCGTCGTCAC
181 ACCCGCACGC ATACTGGCGA AAAGCCTTTC CAGTGTCGCA TTTGCATGCG CAATTTTTCC
241 GTTCGTCATA ATTTAACGCG CCACCTTCGC ACGCACACCG GTGAGAAACC GTTCCAATGC
301 CGTATCTGTA TGCGTAACTT TTCGGACCGC ACTAGTCTGG CGCGACATTT GAAAACCCAT
361 TATCCCTATG ATGTGCCGGA TTACGCTggt ggaagtggag gtggaagtgg aggtagtATG
421 ACGATCAGTT CCCCGCTGAT CGATGTTGCT AACCTGCCGG ATATTAACAC CACGGCGGGA
481 AAAATAGCTG ATCTCAAAGC GCGCCGCGCG GAAGCCCATT TCCCTATGGG CGAAAAGGCG
541 GTTGAGAAAG TGCACGCCGC AGGTCGTCTG ACAGCACGGG AACGTCTGGA TTATCTCTTA
601 GATGAAGGCT CTTTCATCGA AACCGATCAA CTTGCCCGCC ACCGGACGAC GGCGTTTGGC
661 CTGGGCGCCA AACGTCCGGC AACCGATGGG ATCGTGACCG GGTGGGGTAC CATTGATGGC
721 CGCGAAGTCT GTATTTTCAG CCAGGATGGA ACTGTTTTTG GCGGCGCGCT GGGCGAGGTT
781 TACGGGGAAA AAATGATAAA AATTATGGAA TTAGCGATCG ATACGGGGCG TCCGCTGATT
841 GGCCTGTACG AGGGAGCGGG CGCCCGAATC CAGGATGGTG CAGTCTCGCT AGATTTCATT
901 AGTCAAACAT TTTACCAGAA TATCCAGGCC AGCGGAGTAA TTCCCCAGAT TAGTGTGATC
961 ATGGGCGCGT GCGCCGGTGG GAATGCTTAT GGCCCAGCGT TGACAGACTT CGTGGTGATG
1021 GTCGATAAGA CGTCTAAAAT GTTTGTGACG GGTCCAGATG TGATTAAGAC CGTGACTGGC
1081 GAGGAAATTA CCCAGGAGGA GCTTGGTGGT GCAACGACAC ACATGGTAAC TGCGGGAAAC
1141 TCCCATTATA CTGCGGCTAC CGACGAGGAA GCCTTGGATT GGGTTCAAGA TCTGGTTAGC
1201 TTTCTGCCTT CCAACAACCG GTCTTACACT CCCCTGGAAG ATTTTGACGA AGAAGAAGGT
1261 GGGGTTGAAG AAAATATCAC CGCAGACGAC CTTAAACTCG ACGAAATCAT TCCGGATTCA
1321 GCTACCGTAC CATACGACGT TCGTGACGTC ATCGAATGTC TTACCGATGA CGGTGAGTAT
1381 TTGGAGATTC AGGCGGACCG CGCCGAAAAC GTCGTGATTG CGTTTGGGCG CATTGAAGGT
1441 CAAAGCGTTG GCTTTGTGGC AAATCAGCCG ACTCAGTTCG CGGGTTGTCT GGATATTGAT
1501 TCATCGGAAA AAGCAGCACG TTTCGTGCGT ACGTGCGATG CGTTTAACAT CCCGATTGTA
1561 ATGCTGGTCG ACGTGCCGGG ATTTCTGCCG GGAGCCGGTC AAGAATATGG TGGTATTCTG
1621 AGACGCGGCG CTAAATTATT ATATGCATAT GGCGAAGCAA CAGTACCGAA AATCACCGTT
1681 ACCATGCGTA AGGCATACGG GGGTGCGTAC TGCGTCATGG GCTCAAAAGG CTTGGGCAGT
1741 GACATCAATC TAGCGTGGCC GACCGCTCAG ATTGCCGTGA TGGGTGCCGC GGGCGCAGTC
1801 GGCTTCATAT ATCGCAAAGA ACTGATGGCG GCCGATGCCA AAGGTCTGGA TACGGTGGCT
1861 CTGGCTAAAA GCTTTGAGCG CGAGTATGAA GACCACATGC TGAATCCTTA TCATGCTGCC
1921 GAGCGTGGGC TCATAGACGC GGTAATCTTA CCCTCGGAAA CCAGGGGCCA GATTAGCCGA
1981 AATCTGCGTC TTCTGAAGCA TAAAAATGTG ACACGCCCAG CCAGAAAACA TGGTAACATG
2041 CCTTTGTAAc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
2101 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
2161 ggcctttctg cgtttatata ctagtagcgg ccgctgcagT GCCAGAGACC CCATTA
//`
sequences.seq_ACS_intro_gblock = {}; sequences.seq_ACS_intro_gblock.varName = "seq_ACS_intro_gblock"; sequences.seq_ACS_intro_gblock.name = "ACS intro gblock"; sequences.seq_ACS_intro_gblock.seqType = "linear"; sequences.seq_ACS_intro_gblock.data = `LOCUS ACS_intro_gblock 458 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 18..39
/label="Prefix"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature 40..74
/label="promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 122..415
/label="ZFB"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
misc_binding 75..98
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 99..118
/label="RBS"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature 416..445
/label="Spacer"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 GTTTCAGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
61 ggtacagtgc tagcTGGAAT TGTGAGCGGA TAACAATTTC GACTCGTTCG ATTAAAGAAT
121 GACCAGTGCG GCTGCCCCTG GTGAACGCCC GTTTCAGTGT CGGATTTGCA TGCGAAATTT
181 CAGCAAAAAA GATCATCTCC ACCGTCATAC TCGCACACAC ACTGGGGAAA AACCGTTCCA
241 GTGTCGCATT TGCATGCGTA ACTTTTCCTT GTCACAAACC CTGAAACGTC ACCTTCGCAC
301 GCATACGGGC GAGAAGCCCT TCCAGTGTAG AATCTGCATG CGCAACTTTT CGCGTTTAGA
361 TATGCTGGCG CGTCATCTGA AAACCCATTA TCCATACGAT GTGCCGGACT ATGCAggtgg
421 aagtggaggt ggaagtggag gtagtAGAGA CCGGACTA
//`
sequences.seq_ACS_outro_gblock = {}; sequences.seq_ACS_outro_gblock.varName = "seq_ACS_outro_gblock"; sequences.seq_ACS_outro_gblock.name = "ACS outro gblock"; sequences.seq_ACS_outro_gblock.seqType = "linear"; sequences.seq_ACS_outro_gblock.data = `LOCUS ACS_outro_gblock 2143 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 2106..2126
/label="Suffix"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 18..1973
/label="ACS"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1977..2105
/label="Terminator"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 GTTTCAGGTC TCATAGTATG TCTCAGATTC ACAAACATAC TATCCCTGCA AACATTGCTG
61 ACCGTTGCCT GATTAATCCT CAACAGTATG AAGCCATGTA CCAGCAGTCG ATTAATGTGC
121 CGGATACATT TTGGGGCGAA CAGGGGAAGA TTCTGGACTG GATCAAACCG TACCAAAAAG
181 TGAAAAATAC CAGTTTCGCA CCGGGTAATG TTAGCATCAA ATGGTACGAA GACGGCACTC
241 TTAACCTTGC GGCGAATTGT CTGGATCGTC ATCTACAAGA AAACGGCGAT CGTACAGCGA
301 TTATATGGGA GGGTGATGAT GCTAGCCAGT CGAAGCATAT CAGCTATAAA GAACTTCATC
361 GCGACGTATG CCGCTTCGCA AACACGTTGT TAGAATTAGG CATCAAGAAA GGTGATGTGG
421 TCGCCATTTA TATGCCAATG GTTCCGGAAG CGGCCGTGGC TATGCTCGCA TGCGCCCGTA
481 TTGGAGCGGT CCATTCGGTT ATTTTTGGGG GGTTTTCCCC GGAGGCAGTC GCCGGCCGCA
541 TTATTGATTC CAATAGCCGT CTCGTCATCA CCTCTGATGA AGGAGTTCGG GCGGGCCGCA
601 GCATTCCTCT GAAAAAGAAT GTAGACGATG CTCTGAAAAA CCCTAACGTC ACGAGTGTCG
661 AGCACGTTGT TGTGTTAAAA CGCACCGGTG GTAAAATTGA CTGGCAGGAA GGGCGTGACT
721 TGTGGTGGCA TGACCTTGTA GAACAAGCGT CCGATCAGCA TCAGGCTGAA GAGATGAACG
781 CAGAGGACCC GCTGTTCATC TTATATACCA GCGGATCTAC AGGTAAACCC AAAGGGGTGC
841 TGCATACTAC GGGCGGTTAT TTGGTGTACG CCGCCCTGAC TTTTAAATAC GTCTTTGATT
901 ACCACCCCGG TGACATCTAT TGGTGTACCG CTGATGTCGG TTGGGTTACC GGACATAGCT
961 ATCTGCTGTA TGGCCCCTTG GCATGTGGGG CCACAACGTT GATGTTTGAA GGAGTGCCGA
1021 ATTGGCCGAC CCCAGCCCGC ATGGCGCAGG TAGTTGATAA GCATCAGGTG AACATCCTGT
1081 ACACGGCTCC GACGGCCATT CGAGCTCTGA TGGCGGAAGG CGATAAAGCG ATAGAAGGCA
1141 CGGATCGCTC TTCTCTCCGC ATCCTGGGTT CAGTTGGCGA GCCAATCAAC CCAGAGGCGT
1201 GGGAGTGGTA CTGGAAAAAA ATTGGTAATG AAAAATGCCC GGTCGTGGAC ACCTGGTGGC
1261 AGACCGAAAC GGGAGGCTTT ATGATTACCC CGCTGCCAGG CGCGACGGAG CTAAAGGCAG
1321 GGAGCGCAAC AAGGCCTTTT TTCGGTGTGC AACCGGCCTT AGTGGACAAT GAGGGTAATC
1381 CGCTGGAAGG GGCGACCGAG GGATCACTGG TGATTACTGA CAGTTGGCCG GGCCAGGCAC
1441 GAACCCTGTT CGGTGATCAC GAAAGATTCG AACAAACGTA TTTCTCCACA TTTAAAAACA
1501 TGTATTTTTC AGGTGACGGC GCGCGTAGAG ATGAAGATGG TTATTATTGG ATCACCGGCC
1561 GGGTCGATGA TGTGCTCAAC GTTAGCGGTC ACCGTCTTGG CACGGCGGAA ATCGAATCGG
1621 CTCTGGTGGC CCACCCTAAG ATCGCGGAGG CAGCCGTGGT AGGCATTCCG CATAACATAA
1681 AAGGCCAGGC CATTTACGCG TATGTGACTT TGAATCACGG CGAAGAACCA TCGCCGGAAT
1741 TATACGCGGA AGTACGCAAC TGGGTTCGGA AAGAAATCGG TCCACTGGCA ACCCCGGATG
1801 TACTGCACTG GACCGATTCA CTGCCCAAAA CGCGCAGTGG GAAGATCATG CGTCGCATAC
1861 TGCGTAAAAT TGCGGCCGGT GATACTTCAA ACCTCGGCGA TACCTCCACC CTTGCAGATC
1921 CCGGCGTAGT TGAAAAACTG CTGGAAGAGA AACAAGCCAT TGCGATGCCG AGTTGAccag
1981 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
2041 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
2101 ttatatacta gtagcggccg ctgcagTGCC AGAGACCGGA CTA
//`
sequences.seq_BsaI_FWD_primer = {}; sequences.seq_BsaI_FWD_primer.varName = "seq_BsaI_FWD_primer"; sequences.seq_BsaI_FWD_primer.name = "BsaI FWD primer"; sequences.seq_BsaI_FWD_primer.seqType = "linear"; sequences.seq_BsaI_FWD_primer.data = `LOCUS BsaI_FWD_primer 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CTCTCAGGTC TCATGCCagt ccggcaaaaa agggc
//`
sequences.seq_BsaI_REV_primer = {}; sequences.seq_BsaI_REV_primer.varName = "seq_BsaI_REV_primer"; sequences.seq_BsaI_REV_primer.name = "BsaI REV primer"; sequences.seq_BsaI_REV_primer.seqType = "linear"; sequences.seq_BsaI_REV_primer.data = `LOCUS BsaI_REV_primer 40 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATCTTAGGTC TCTCTCCcag aaatcatcct tagcgaaagc
//`
sequences.seq_CoA_4x_Assembly = {}; sequences.seq_CoA_4x_Assembly.varName = "seq_CoA_4x_Assembly"; sequences.seq_CoA_4x_Assembly.name = "CoA 4x Assembly"; sequences.seq_CoA_4x_Assembly.seqType = "both"; sequences.seq_CoA_4x_Assembly.data = `LOCUS CoA_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AA
//`
sequences.seq_CoA_4x_Comp_Assembly = {}; sequences.seq_CoA_4x_Comp_Assembly.varName = "seq_CoA_4x_Comp_Assembly"; sequences.seq_CoA_4x_Comp_Assembly.name = "CoA 4x Comp Assembly"; sequences.seq_CoA_4x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_4x_Comp_Assembly.data = `LOCUS CoA_4x_Comp_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2425..2433)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2480..2488)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2469..2477)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2447..2455)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2414..2422)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2403..2411)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2436..2444)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2392..2400)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2458..2466)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
2461 AGCCGCATGG CATCGACATG CCGTCCTCAA AA
//`
sequences.seq_CoA_4x_Full_Part = {}; sequences.seq_CoA_4x_Full_Part.varName = "seq_CoA_4x_Full_Part"; sequences.seq_CoA_4x_Full_Part.name = "CoA 4x Full Part"; sequences.seq_CoA_4x_Full_Part.seqType = "both"; sequences.seq_CoA_4x_Full_Part.data = `LOCUS CoA_4x_Full_Part 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2779..2787)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
HIV-RT Binding Site 2489..2598
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2746..2754)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Scar 2668..2675
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site join(2832..2835,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2735..2743)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2768..2776)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2790..2798)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2801..2809)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Promoter 2676..2710
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2812..2820)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gBlock 2545..2669
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2757..2765)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 2599..2667
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 2712..2734
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2823..2831)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTCCCAGC CGCATGGCAT CGACATCCCA
2761 GCCGCATGGC ATCGACATCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
2821 ATGCCGTCCT CAAAA
//`
sequences.seq_CoA_4x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.varName = "seq_CoA_4x_r_oligo_Complement_Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.name = "CoA 4x r_oligo Complement Duplex"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Complement_Duplex.data = `LOCUS CoA_4x_r_oligo_Complemen 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(42..50)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(53..61)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(64..72)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(75..83)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(86..94)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(97..105)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(108..116)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(119..127)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(130..138)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(42..138)
/label="Temp2"
/note="sequence: GAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 42..138
/label="Temp"
/note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTC"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
121 CATCGACATG CCGTCCTCAA AACGAGACCA ACCTG
//`
sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.varName = "seq_CoA_4x_r_oligo_Duplex_126145_REV_1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.name = "CoA 4x r_oligo Duplex 126-145 REV 1"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_126145_REV_1.data = `LOCUS CoA_4x_r_oligo_Duplex_126 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGTTTTCCC AGCCGCATGG
//`
sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1 = {}; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.varName = "seq_CoA_4x_r_oligo_Duplex_6988_FWD_1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.name = "CoA 4x r_oligo Duplex 69-88 FWD 1"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex_6988_FWD_1.data = `LOCUS CoA_4x_r_oligo_Duplex_69- 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TGGGATGTCG ATGCCATGCG
//`
sequences.seq_CoA_4x_r_oligo_Duplex = {}; sequences.seq_CoA_4x_r_oligo_Duplex.varName = "seq_CoA_4x_r_oligo_Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.name = "CoA 4x r_oligo Duplex"; sequences.seq_CoA_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_4x_r_oligo_Duplex.data = `LOCUS CoA_4x_r_oligo_Duplex 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 53..61
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 64..72
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 75..83
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 86..94
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 97..105
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 108..116
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 119..127
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 130..138
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(126..145)
/label="CoA 4x r_oligo Duplex 126-145 REV 1"
/note="sequence: TCGTTTTCCCAGCCGCATGG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 18..138
/label="Temp Fwd"
/note="sequence: TGGAATTGTGAGCGGATAACAATTGAGGACGGCATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGGATGTCGATGCCATGCGGCTGGG"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(20..140)
/label="Temp Rev"
/note="sequence: CCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATCCCAGCCGCATGGCATCGACATGCCGTCCTCAATTGTTATCCGCTCACAATTCCA"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer 69..88
/label="CoA 4x r_oligo Duplex 69-88 FWD 1"
/note="sequence: TGGGATGTCGATGCCATGCG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
121 CGATGCCATG CGGCTGGGAA AACGAGACCA ACCTG
//`
sequences.seq_CoA_6x_Assembly = {}; sequences.seq_CoA_6x_Assembly.varName = "seq_CoA_6x_Assembly"; sequences.seq_CoA_6x_Assembly.name = "CoA 6x Assembly"; sequences.seq_CoA_6x_Assembly.seqType = "both"; sequences.seq_CoA_6x_Assembly.data = `LOCUS CoA_6x_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2502..2510
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2491..2499
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2524..2532
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2513..2521
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
HIV-RT Binding Site join(2533..2536,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
2521 CATGCGGCTG GGAAAA
//`
sequences.seq_CoA_6x_Comp_Assembly = {}; sequences.seq_CoA_6x_Comp_Assembly.varName = "seq_CoA_6x_Comp_Assembly"; sequences.seq_CoA_6x_Comp_Assembly.name = "CoA 6x Comp Assembly"; sequences.seq_CoA_6x_Comp_Assembly.seqType = "both"; sequences.seq_CoA_6x_Comp_Assembly.data = `LOCUS CoA_6x_Comp_Assembly 2536 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site complement(2491..2499)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2502..2510)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2480..2488)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2458..2466)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2447..2455)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site complement(2436..2444)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2425..2433)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2414..2422)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2403..2411)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2392..2400)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2513..2521)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2524..2532)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
HIV-RT Binding Site join(2533..2536,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2469..2477)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC
2401 ATGGCATCGA CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC
2461 AGCCGCATGG CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA
2521 CATGCCGTCC TCAAAA
//`
sequences.seq_CoA_6x_Full_Part = {}; sequences.seq_CoA_6x_Full_Part.varName = "seq_CoA_6x_Full_Part"; sequences.seq_CoA_6x_Full_Part.name = "CoA 6x Full Part"; sequences.seq_CoA_6x_Full_Part.seqType = "both"; sequences.seq_CoA_6x_Full_Part.data = `LOCUS CoA_6x_Full_Part 2923 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site 2425..2433
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_binding 2756..2778
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2790..2798)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2469..2477
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2900..2908)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2480..2488
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2447..2455
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2867..2875)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2533..2642
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2524..2532
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site complement(2823..2831)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2458..2466
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2812..2820)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2436..2444
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2878..2886)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2491..2499
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2856..2864)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2502..2510
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Terminator 2643..2711
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2834..2842)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2513..2521
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(2779..2787)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site 2403..2411
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 2720..2754
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2845..2853)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2712..2719
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2414..2422
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 2589..2713
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2889..2897)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2801..2809)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site join(2920..2923,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2911..2919)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGTCGATGC CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG
2461 GCTGGGATGT CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC
2521 CATGCGGCTG GGAAAAAAAA CGTGGCGCCC GAACAGGGAC GGATAGCTCA GTCGGTAGAG
2581 CATCAGACTT TTAATCTGAG GGTCCAGGGT TCAAGTCCCT GTTCGGGCGC CACGTTTTTT
2641 TTattagcag aaagtcaaaa gcctccgacc ggaggctttt gactaaaact tcccttgggg
2701 ttatcattgg gtactagagT TGACGGCTAG CTCAGTCCTA GGTACAGTGC TAGCTGGAAT
2761 TGTGAGCGGA TAACAATTCC CAGCCGCATG GCATCGACAT CCCAGCCGCA TGGCATCGAC
2821 ATCCCAGCCG CATGGCATCG ACATCCCAGC CGCATGGCAT CGACATCCCA GCCGCATGGC
2881 ATCGACATCC CAGCCGCATG GCATCGACAT GCCGTCCTCA AAA
//`
sequences.seq_CoA_6x_r_oligo_Complement_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.varName = "seq_CoA_6x_r_oligo_Complement_Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.name = "CoA 6x r_oligo Complement Duplex"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Complement_Duplex.data = `LOCUS CoA_6x_r_oligo_Complemen 199 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(42..50)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(53..61)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(64..72)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(75..83)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(86..94)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(97..105)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(108..116)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(119..127)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(130..138)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(141..149)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(152..160)
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(163..171)
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site complement(174..182)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TCCCAGCCGC ATGGCATCGA
61 CATCCCAGCC GCATGGCATC GACATCCCAG CCGCATGGCA TCGACATCCC AGCCGCATGG
121 CATCGACATC CCAGCCGCAT GGCATCGACA TCCCAGCCGC ATGGCATCGA CATGCCGTCC
181 TCAAAACGAG ACCAACCTG
//`
sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.varName = "seq_CoA_6x_r_oligo_Duplex_113132_FWD_1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.name = "CoA 6x r_oligo Duplex 113-132 FWD 1"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_113132_FWD_1.data = `LOCUS CoA_6x_r_oligo_Duplex_113 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TGGGATGTCG ATGCCATGCG
//`
sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1 = {}; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.varName = "seq_CoA_6x_r_oligo_Duplex_170189_REV_1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.name = "CoA 6x r_oligo Duplex 170-189 REV 1"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex_170189_REV_1.data = `LOCUS CoA_6x_r_oligo_Duplex_170 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGTTTTCCC AGCCGCATGG
//`
sequences.seq_CoA_6x_r_oligo_Duplex = {}; sequences.seq_CoA_6x_r_oligo_Duplex.varName = "seq_CoA_6x_r_oligo_Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.name = "CoA 6x r_oligo Duplex"; sequences.seq_CoA_6x_r_oligo_Duplex.seqType = "linear"; sequences.seq_CoA_6x_r_oligo_Duplex.data = `LOCUS CoA_6x_r_oligo_Duplex 199 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 53..61
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 64..72
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 75..83
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 86..94
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 97..105
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 108..116
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 119..127
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 130..138
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 141..149
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 152..160
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 163..171
/label="ZFa Site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 174..182
/label="ZFb Site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 113..132
/label="CoA 6x r_oligo Duplex 113-132 FWD 1"
/note="sequence: TGGGATGTCGATGCCATGCG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(170..189)
/label="CoA 6x r_oligo Duplex 170-189 REV 1"
/note="sequence: TCGTTTTCCCAGCCGCATGG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGTCGATGC
61 CATGCGGCTG GGATGTCGAT GCCATGCGGC TGGGATGTCG ATGCCATGCG GCTGGGATGT
121 CGATGCCATG CGGCTGGGAT GTCGATGCCA TGCGGCTGGG ATGTCGATGC CATGCGGCTG
181 GGAAAACGAG ACCAACCTG
//`
sequences.seq_gBlock_A = {}; sequences.seq_gBlock_A.varName = "seq_gBlock_A"; sequences.seq_gBlock_A.name = "gBlock A"; sequences.seq_gBlock_A.seqType = "linear"; sequences.seq_gBlock_A.data = `LOCUS gBlock_A 995 bp ds-DNA linear 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 863..870
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 785..790
/label="double stop codon"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 18..979
/label="gBlock A"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
stem_loop 883..905
/label="stem loop"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature 110..815
/label="RFP"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 82..89
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 97..101
/label="strong RBS"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 110..815
/label="BBa_E1010 (RFP)"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 110..112
/label="start codon"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 90..104
/label="BBa_B0030 (strong RBS)"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 822..862
/label="BBa_B0012"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 822..916
/label="BBa_B0014 (double terminator)"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 871..916
/label="BBa_B0011"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 18..39
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 90..916
/label="From BBa_K3114015"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 794..799
/label="double stop codon"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
misc_feature 40..74
/label="BBa_J23105"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 GACTTACGTC TCGtctggaa ttcgcggccg cttctagagt ttacggctag ctcagtccta
61 ggtactatgc tagcCGAGAC Ctactagaga ttaaagagga gaaatactaa tggcttcctc
121 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
181 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
241 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
301 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
361 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
421 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
481 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
541 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
601 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
661 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
721 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
781 tgcttaataa cgctgatagt gctagtgtag atcgctaata atcacactgg ctcaccttcg
841 ggtgggcctt tctgcgttta tatactagag agagaatata aaaagccaga ttattaatcc
901 ggctttttta ttatttGGTC TCGAAAAAAA ACGTGGCGCC CGAACAGGGA CGGATAGCTC
961 AGTCGGTAGA GCATCAGACT TTCGAGACGC TTGTG
//`
sequences.seq_gBlock_B = {}; sequences.seq_gBlock_B.varName = "seq_gBlock_B"; sequences.seq_gBlock_B.name = "gBlock B"; sequences.seq_gBlock_B.seqType = "linear"; sequences.seq_gBlock_B.data = `LOCUS gBlock_B 175 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 138..158
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 15..158
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc 72..111
/label="identical to E. coli MG1655 between tonB and yciA"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 69..137
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
stem_loop 79..110
/label="stem loop"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 GATCGTCGTC TCCCTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC GGGCGCCACG
61 TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact aaaacttccc
121 ttggggttat cattgggtac tagtagcggc cgctgcagag tcCGAGACGT TCCAT
//`
sequences.seq_GFP_final_synthesized_for_pSB1T3_linear = {}; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.varName = "seq_GFP_final_synthesized_for_pSB1T3_linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.name = "GFP final synthesized for pSB1T3 linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.seqType = "linear"; sequences.seq_GFP_final_synthesized_for_pSB1T3_linear.data = `LOCUS GFP_final_synthesized_f 1240 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 91..405
/label="Zif268 + linker"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Recognition Sequence 7..12
/label="BsaI 5' site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1..6
/label="rando bases"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 1235..1240
/label="rando bases"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
misc_feature 18..52
/label="new promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 406..1122
/label="GFP"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Recognition Sequence 1229..1234
/label="BsaI 3' site"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Scar 1170..1177
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 1129..1223
/label="BBa_B0014 Double Terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 77..90
/label="RBS - J61111"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_binding 54..76
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 GATACGGGTC TCACGTTttt acggctagct cagtcctagg tatagtgcta gcTGGAATTG
61 TGAGCGGATA ACAATTGAAA GAGGCGATAC ATGCACCATC ACCATCATCA CCCGGGTGAG
121 AAGCCATATG CCTGCCCCGT GGAAAGCTGC GATCGTCGTT TCAGTCGCTC TGATGAACTG
181 ACACGCCACA TCAGAATACA TACCGGGCAG AAACCGTTTC AATGTCGCAT TTGTATGCGC
241 AACTTTTCGC GCAGCGACCA TTTAACTACG CATATCCGTA CCCACACTGG TGAAAAACCT
301 TTCGCGTGTG ATATTTGCGG ACGAAAATTT GCACGGTCAG ACGAGCGTAA ACGTCATACC
361 AAAATTCATA CGGGCGGCGG CAGCGGCGGC GGTAGTGGTG GGTCCcgtaa aggagaagaa
421 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
481 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
541 atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat
601 ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt
661 gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac
721 aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa
781 ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac
841 tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa
901 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
961 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
1021 ctttcgaaag atcccaacga aaagAGGgac cacatggtcc ttcttgagtt tgtaacagct
1081 gctgggatta cacatggcat ggatgaacta tacaaataat aatggctgtc acactggctc
1141 accttcgggt gggcctttct gcgtttatat actagagaga gaatataaaa agccagatta
1201 ttaatccggc ttttttatta tttTGCCaGA GACCAGGCTT
//`
sequences.seq_GFP_Fusion_in_TetR = {}; sequences.seq_GFP_Fusion_in_TetR.varName = "seq_GFP_Fusion_in_TetR"; sequences.seq_GFP_Fusion_in_TetR.name = "GFP Fusion in TetR"; sequences.seq_GFP_Fusion_in_TetR.seqType = "both"; sequences.seq_GFP_Fusion_in_TetR.data = `LOCUS GFP_Fusion_in_TetR 3675 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 2539..2853
/label="Zif268 + linker"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 2466..2500
/label="new promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
terminator 2394..2437
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2525..2538
/label="RBS - J61111"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer_bind complement(103..120)
/label="L4440"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 2440..2461
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 3672..3675
/label="3' overhang"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Scar 3618..3625
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 22..79
/label="his operon terminator"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 3577..3671
/label="BBa_B0014 Double Terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Biobricking 1..21
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 2854..3570
/label="GFP"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 1..21
/label="BioBrick suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 2372..2390
/label="pBRforEco"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(274..862)
/label="ori"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 2462..2465
/label="5' overhang"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind complement(354..373)
/label="pBR322ori-F"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS 1128..2312
/label="TetR"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2440..2461
/label="BioBrick prefix"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer complement(2444..2461)
/label="Copy of pSB1T3 REV"
/note="sequence: AAGGTCGGTCTCCCTCTctctagaagcggccgcga"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 1..18
/label="Copy of pSB1T3 FWD"
/note="sequence: AAGGTCGGTCTCCTACTtactagtagcggccgctg"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
1021 ttggtctgac agctcgagat tctcatgttt gacagcttat catcgataag ctttaatgcg
1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
1261 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
1321 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
1381 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
1441 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
1501 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
1561 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
1621 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
1681 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
1741 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
1801 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
1861 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
1921 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
1981 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
2041 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
2101 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
2161 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
2221 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
2281 gtcgcggtgc atggagccgg gccacctcga cctaactcga gtgccacctg acgtctaaga
2341 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
2401 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg aattcgcggc cgcttctaga
2461 gAGAGtttac ggctagctca gtcctaggta tagtgctagc TGGAATTGTG AGCGGATAAC
2521 AATTGAAAGA GGCGATACAT GCACCATCAC CATCATCACC CGGGTGAGAA GCCATATGCC
2581 TGCCCCGTGG AAAGCTGCGA TCGTCGTTTC AGTCGCTCTG ATGAACTGAC ACGCCACATC
2641 AGAATACATA CCGGGCAGAA ACCGTTTCAA TGTCGCATTT GTATGCGCAA CTTTTCGCGC
2701 AGCGACCATT TAACTACGCA TATCCGTACC CACACTGGTG AAAAACCTTT CGCGTGTGAT
2761 ATTTGCGGAC GAAAATTTGC ACGGTCAGAC GAGCGTAAAC GTCATACCAA AATTCATACG
2821 GGCGGCGGCA GCGGCGGCGG TAGTGGTGGG TCCcgtaaag gagaagaact tttcactgga
2881 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
2941 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
3001 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
3061 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
3121 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
3181 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
3241 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
3301 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
3361 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
3421 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
3481 cccaacgaaa agAGGgacca catggtcctt cttgagtttg taacagctgc tgggattaca
3541 catggcatgg atgaactata caaataataa tggctgtcac actggctcac cttcgggtgg
3601 gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt aatccggctt
3661 ttttattatt tTACT
//`
sequences.seq_GGE114 = {}; sequences.seq_GGE114.varName = "seq_GGE114"; sequences.seq_GGE114.name = "GGE114"; sequences.seq_GGE114.seqType = "both"; sequences.seq_GGE114.data = `LOCUS (Addgene_#120731_)_GGE1 5268 bp ds-DNA circular 06-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
terminator 4070..4113
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2002..4115
/label="pSB1A3 Region"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
protein_bind 4452..4485
/label="loxP"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
source 1..5268
/label="source"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind 3638..3657
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
promoter complement(3876..3980)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS 722..1399
/label="mRFP1"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS complement(3015..3875)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
rep_origin complement(2256..2844)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
promoter 632..662
/label="lac promoter"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(2085..2102)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
terminator 2004..2061
/label="his operon terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
protein_bind 670..686
/label="lac operator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
protein_bind 596..617
/label="CAP binding site"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
terminator 1444..1515
/label="rrnB T1 terminator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind 4048..4066
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(2336..2355)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 3156..3156
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 1531..1558
/label="T7Te terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
RBS 704..715
/label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer 2002..2019
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(4088..4115)
/label="BBa_Prefix & Promoter SDM Rev"
/note="sequence: cagaaatcatccttagcgaaagctaagg"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer complement(4093..4115)
/label="BsaI pSB1A3 Rev"
/note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 4116..4135
/label="BBa_Prefix & Promoter SDM Fwd"
/note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 2002..2019
/label="BsaI pSB1A3 Fwd"
/note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(4093..4115)
/label="Linear pSB1A3 BsmBI Rev"
/note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(4093..4115)
/label="pSB1A3 SapI rev"
/note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 2002..2019
/label="pSB1A3 fwd SapI"
/note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 2002..2023
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
ORIGIN
1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
//`
sequences.seq_Linear_pSB1A3_BsmBI_Fwd = {}; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.varName = "seq_Linear_pSB1A3_BsmBI_Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.name = "Linear pSB1A3 BsmBI Fwd"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Fwd.data = `LOCUS Linear_pSB1A3_BsmBI_Fwd 31 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CTATTTCGTC TCCagtccgg caaaaaaggg c
//`
sequences.seq_Linear_pSB1A3_BsmBI_Rev = {}; sequences.seq_Linear_pSB1A3_BsmBI_Rev.varName = "seq_Linear_pSB1A3_BsmBI_Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.name = "Linear pSB1A3 BsmBI Rev"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.seqType = "linear"; sequences.seq_Linear_pSB1A3_BsmBI_Rev.data = `LOCUS Linear_pSB1A3_BsmBI_Rev 36 bp ds-DNA linear 20-JUN-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATTCCCCGTC TCCcagaaat catccttagc gaaagc
//`
sequences.seq_Linear_pSB1A3_with_BsmBI_Sites = {}; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.varName = "seq_Linear_pSB1A3_with_BsmBI_Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.name = "Linear pSB1A3 with BsmBI Sites"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.seqType = "linear"; sequences.seq_Linear_pSB1A3_with_BsmBI_Sites.data = `LOCUS Linearized_pSB1A3_w/_Bs 2140 bp ds-DNA linear 24-JUN-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
CDS complement(1027..1887)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 1650..1669
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind complement(97..114)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer_bind 2060..2078
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer_bind complement(348..367)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 14..2127
/label="pSB1A3 Region"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1168..1168
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2082..2125
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
terminator 16..73
/label="his operon terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
promoter complement(1888..1992)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
rep_origin complement(268..856)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer complement(2100..2127)
/label="BBa_Prefix & Promoter SDM Rev"
/note="sequence: cagaaatcatccttagcgaaagctaagg"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 14..35
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
ORIGIN
1 CTATTTCGTC TCCagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
61 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
121 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
181 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
241 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
301 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
361 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
421 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
481 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
541 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
601 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
661 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
721 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
781 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
841 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
901 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
961 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
1021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
1081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
1141 tggccccagt gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc
1201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
1261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
1321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
1381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
1441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
1501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
1561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
1621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
1681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
1741 gagatccagt tcgatataac ccactcgtgc acccaactga tcttcagcat cttttacttt
1801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
1861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
1921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
1981 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
2041 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
2101 cttagctttc gctaaggatg atttctgGGA GACGGGGAAT
//`
sequences.seq_pAC4CL1_FWD = {}; sequences.seq_pAC4CL1_FWD.varName = "seq_pAC4CL1_FWD"; sequences.seq_pAC4CL1_FWD.name = "pAC-4CL1 FWD"; sequences.seq_pAC4CL1_FWD.seqType = "linear"; sequences.seq_pAC4CL1_FWD.data = `LOCUS pAC-4CL1_FWD 39 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTCCCAGCTC TTCCagtatg gaggaggatt acaaaatgg
//`
sequences.seq_pAC4CL1_REV = {}; sequences.seq_pAC4CL1_REV.varName = "seq_pAC4CL1_REV"; sequences.seq_pAC4CL1_REV.name = "pAC-4CL1 REV"; sequences.seq_pAC4CL1_REV.seqType = "linear"; sequences.seq_pAC4CL1_REV.data = `LOCUS pAC-4CL1_REV 39 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTCCCAGCTC TTCCcaatca caatccattt gctagtttt
//`
sequences.seq_pAC4CL1 = {}; sequences.seq_pAC4CL1.varName = "seq_pAC4CL1"; sequences.seq_pAC4CL1.name = "pAC-4CL1"; sequences.seq_pAC4CL1.seqType = "both"; sequences.seq_pAC4CL1.data = `LOCUS pAC-4CL1 6156 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "marker:CmR" "ref:pmid\:16551366" FEATURES Location/Qualifiers
CDS complement(join(5716..6156,1..219))
/label="CmR"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter 1969..1975
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
protein_bind 1909..1930
/label="CAP binding site"
/ApEinfo_revcolor=#31849b
/ApEinfo_fwdcolor=#31849b
CDS complement(join(5719..6156,1..219))
/label="CmR"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
promoter 1504..1509
/label="tet promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
rep_origin complement(848..1392)
/label="p15A ori"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
promoter 1951..1968
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 2016..3719
/label="4CL"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
promoter 1527..1532
/label="tet promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 1945..1950
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter complement(220..322)
/label="cat promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 1510..1526
/label="tet promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 1945..1975
/label="lac promoter"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
961 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
1021 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
1081 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
1141 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
1201 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
1261 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
1321 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
1381 aaacgatctc aagaagatca tcttattaat cagataaaat atttcaagat ttcagtgcaa
1441 tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca
1501 tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta
1561 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
1621 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
1681 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
1741 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
1801 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
1861 cgtcctgtgg atccccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
1921 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
1981 aattgtgagc gtctagagcg cccggggaat tccccatgga ggaggattac aaaatggcgc
2041 cacaagaaca agcagtttct caggtgatgg agaaacagag caacaacaac aacagtgacg
2101 tcattttccg atcaaagtta ccggatattt acatcccgaa ccacctatct ctccacgact
2161 acatcttcca aaacatctcc gaattcgcca ctaagccttg cctaatcaac ggaccaaccg
2221 gccacgtgta cacttactcc gacgtccacg tcatctcccg ccaaatcgcc gccaattttc
2281 acaaactcgg cgttaaccaa aacgacgtcg tcatgctcct cctcccaaac tgtcccgaat
2341 tcgtcctctc tttcctcgcc gcctccttcc gcggcgcaac cgccaccgcc gcaaaccctt
2401 tcttcactcc ggcggagata gctaaacaag ccaaagcctc caacaccaaa ctcataatca
2461 ccgaagctcg ttacgtcgac aaaatcaaac cacttcaaaa cgacgacgga gtagtcatcg
2521 tctgcatcga cgacaacgaa tccgtgccaa tccctgaagg ctgcctccgc ttcaccgagt
2581 tgactcagtc gacaaccgag gcatcagaag tcatcgactc ggtggagatt tcaccggacg
2641 acgtggtggc actaccttac tcctctggca cgacgggatt accaaaagga gtgatgctga
2701 ctcacaaggg actagtcacg agcgttgctc agcaagtcga cggcgagaac ccgaatcttt
2761 atttccacag cgatgacgtc atactctgtg ttttgcccat gtttcatatc tacgctttga
2821 actcgatcat gttgtgtggt cttagagttg gtgcggcgat tctgataatg ccgaagtttg
2881 agatcaatct gctattggag ctgatccaga ggtgtaaagt gacggtggct ccgatggttc
2941 cgccgattgt gttggccatt gcgaagtctt cggagacgga gaagtatgat ttgagctcga
3001 taagagtggt gaaatctggt gctgctcctc ttggtaaaga acttgaagat gccgttaatg
3061 ccaagtttcc taatgccaaa ctcggtcagg gatacggaat gacggaagca ggtccagtgc
3121 tagcaatgtc gttaggtttt gcaaaggaac cttttccggt taagtcagga gcttgtggta
3181 ctgttgtaag aaatgctgag atgaaaatag ttgatccaga caccggagat tctctttcga
3241 ggaatcaacc cggtgagatt tgtattcgtg gtcaccagat catgaaaggt tacctcaaca
3301 atccggcagc tacagcagag accattgata aagacggttg gcttcatact ggagatattg
3361 gattgatcga tgacgatgac gagcttttca tcgttgatcg attgaaagaa cttatcaagt
3421 ataaaggttt tcaggtagct ccggctgagc tagaggcttt gctcatcggt catcctgaca
3481 ttactgatgt tgctgttgtc gcaatgaaag aagaagcagc tggtgaagtt cctgttgcat
3541 ttgtggtgaa atcgaaggat tcggagttat cagaagatga tgtgaagcaa ttcgtgtcga
3601 aacaggttgt gttttacaag agaatcaaca aagtgttctt cactgaatcc attcctaaag
3661 ctccatcagg gaagatattg aggaaagatc tgagggcaaa actagcaaat ggattgtgac
3721 catgggcggc cgctgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3781 ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
3841 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
3901 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
3961 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
4021 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct
4081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
4141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
4201 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
4261 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
4321 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
4381 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
4441 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
4501 aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg
4561 cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc
4621 tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct
4681 gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca
4741 attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc
4801 cgccatctcc agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt
4861 gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg
4921 ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc
4981 tgcgacctga gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac
5041 gcggaagtcc cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga
5101 taccacgata ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt
5161 acaacagtcc gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt
5221 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc
5281 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac
5341 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
5401 tgtattaacg aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata
5461 tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag
5521 cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg
5581 gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc
5641 cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata
5701 actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt
5761 aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg
5821 catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa
5881 gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga
5941 gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca
6001 cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca
6061 gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc
6121 ccatatcacc agctcaccgt ctttcattgc catacg
//`
sequences.seq_pduABJKNUT_Fwd = {}; sequences.seq_pduABJKNUT_Fwd.varName = "seq_pduABJKNUT_Fwd"; sequences.seq_pduABJKNUT_Fwd.name = "pduABJKNUT Fwd"; sequences.seq_pduABJKNUT_Fwd.seqType = "linear"; sequences.seq_pduABJKNUT_Fwd.data = `LOCUS pduABJKNUT_Fwd 46 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCTGCTCGTC TCTgaccaca acggtttccc tgtagaaata attttg
//`
sequences.seq_pduABJKNUT_promoter = {}; sequences.seq_pduABJKNUT_promoter.varName = "seq_pduABJKNUT_promoter"; sequences.seq_pduABJKNUT_promoter.name = "pduABJKNUT promoter"; sequences.seq_pduABJKNUT_promoter.seqType = "linear"; sequences.seq_pduABJKNUT_promoter.data = `LOCUS *pduABJKNUT_promoter 133 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 38..72
/label="BBa_J23114"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 34..37
/label="Overhang pSB1C3"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 97..100
/label="Overhang pduABJKNUT"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
misc_binding 74..96
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 AAGTGTTCGA ACGCTTCAGA TCTGTACGTC TCACAACttt atggctagct cagtcctagg
61 tacaatgcta gcTGGAATTG TGAGCGGATA ACAATTGACC AGAGACGTCT GCTTGCCAAC
121 CGTAGAACTT ACG
//`
sequences.seq_pduABJKNUT_Rev = {}; sequences.seq_pduABJKNUT_Rev.varName = "seq_pduABJKNUT_Rev"; sequences.seq_pduABJKNUT_Rev.name = "pduABJKNUT Rev"; sequences.seq_pduABJKNUT_Rev.seqType = "linear"; sequences.seq_pduABJKNUT_Rev.data = `LOCUS pduABJKNUT_Rev 42 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACTGTTCGTC TCAgctttgt tagcagccgg atccaaaagt ag
//`
sequences.seq_PduD_final_product = {}; sequences.seq_PduD_final_product.varName = "seq_PduD_final_product"; sequences.seq_PduD_final_product.name = "PduD (final product)"; sequences.seq_PduD_final_product.seqType = "linear"; sequences.seq_PduD_final_product.data = `LOCUS *PduD_(final_product) 658 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 115..154
/label="random bp"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 18..112
/label="BBa_B0014 Double Terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 653..658
/label="rando runway"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature 218..231
/label="Anderson RBS 7"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Scar 59..66
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 583..621
/label="BBa_B1006"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 1..6
/label="Rando Runway"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 286..582
/label="Spacer + ZFc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
misc_feature 14..17
/label="5' overhang with pdu"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 159..193
/label="J23101 Anderson Promoter"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature 232..285
/label="pduD (1-18 aa)"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_binding 195..217
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 TGAGTACGTC TCAAAGCtca cactggctca ccttcgggtg ggcctttctg cgtttatata
61 ctagagagag aatataaaaa gccagattat taatccggct tttttattat tttgAAGGGA
121 GTAAGTCTTC GAATGGTTCA GTTATAACTA TATAgctgtt tacagctagc tcagtcctag
181 gtattatgct agcTGGAATT GTGAGCGGAT AACAATTGAA AGAAGAGACT CATGGAAATC
241 AACGAAAAAC TGCTGCGTCA GATTATTGAG GACGTGTTAC GCGATACGTC GGCGGCCGCA
301 CCAGGCGAAC GCCCATTTCA GTGTCGCATC TGCATGCGTA ATTTCTCTTC ACCGAGCAAA
361 CTTATTCGCC ATACCCGTAC CCATACTGGC GAGAAACCCT TTCAGTGTCG TATCTGTATG
421 CGAAACTTCT CCGACGGGAG CAACCTGGCT CGGCACTTGC GTACACATAC CGGTGAAAAG
481 CCGTTTCAAT GCCGTATTTG CATGCGCAAT TTCAGTCGCG TGGATAACTT ACCGAGACAC
541 CTGAAAACGC ACTACCCGTA TGATGTTCCT GATTATGCGT AAaaaaaaaa accccgcccc
601 tgacagggcg gggttttttt tAATTAAAGA CACAAGTTTC CGTCAAGAGA CGTCGAGT
//`
sequences.seq_pduD_on_pSB1C3__Final_Part = {}; sequences.seq_pduD_on_pSB1C3__Final_Part.varName = "seq_pduD_on_pSB1C3__Final_Part"; sequences.seq_pduD_on_pSB1C3__Final_Part.name = "pduD on pSB1C3 - Final Part"; sequences.seq_pduD_on_pSB1C3__Final_Part.seqType = "both"; sequences.seq_pduD_on_pSB1C3__Final_Part.data = `LOCUS pduD_on_pSB1C3_-_Final_ 2589 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind complement(333..352)
/label="pBR322ori-F"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Biobricking 2569..2584
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 2028..2028
/label="BioBrick prefix"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
misc_feature 2177..2473
/label="Spacer + ZFc"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
primer_bind complement(82..99)
/label="L4440"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
source 1..2028
/label="source"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2050..2084
/label="new promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
terminator 1982..2025
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Scar 2515..2522
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
rep_origin complement(253..841)
/label="ori"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS complement(1139..1798)
/label="CmR"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
misc_feature join(2585..2589,1..2028)
/label="pSB1C3 Backbone Region"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_feature 2585..2589
/label="BioBrick suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
terminator 1..58
/label="his operon terminator"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer_bind 1713..1732
/label="CAT-R"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
misc_feature 2123..2176
/label="pduD (1-18 aa)"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Biobricking 2029..2049
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 1960..1978
/label="pBRforEco"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 2474..2568
/label="BBa_B0014 Double Terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Biobricking 2569..2589
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
terminator 1024..1118
/label="lambda t0 terminator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Biobricking 2028..2049
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
source 2585..2589
/label="source"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
misc_feature 2109..2122
/label="RBS-J61107"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
61 acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
121 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
241 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
961 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gctcgaggct
1021 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
1081 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
1141 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
1201 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
1261 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
1321 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
1381 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
1441 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
1501 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
1561 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
1621 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
1681 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
1741 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
1801 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
1861 tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt gccacctgac
1921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
1981 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
2041 cttctagagt ttacagctag ctcagtccta ggtattatgc tagcTGGAAT TGTGAGCGGA
2101 TAACAATTGA AAGAAGAGAC TCATGGAAAT CAACGAAAAA CTGCTGCGTC AGATTATTGA
2161 GGACGTGTTA CGCGATACGT CGGCGGCCGC ACCAGGCGAA CGCCCATTTC AGTGTCGCAT
2221 CTGCATGCGT AATTTCTCTT CACCGAGCAA ACTTATTCGC CATACCCGTA CCCATACTGG
2281 CGAGAAACCC TTTCAGTGTC GTATCTGTAT GCGAAACTTC TCCGACGGGA GCAACCTGGC
2341 TCGGCACTTG CGTACACATA CCGGTGAAAA GCCGTTTCAA TGCCGTATTT GCATGCGCAA
2401 TTTCAGTCGC GTGGATAACT TACCGAGACA CCTGAAAACG CACTACCCGT ATGATGTTCC
2461 TGATTATGCG TAAtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
2521 agagagaata taaaaagcca gattattaat ccggcttttt tattatttta ctagtagcgg
2581 ccgctgcag
//`
sequences.seq_pSB1A3GB3_backbone = {}; sequences.seq_pSB1A3GB3_backbone.varName = "seq_pSB1A3GB3_backbone"; sequences.seq_pSB1A3GB3_backbone.name = "pSB1A3-GB3 backbone"; sequences.seq_pSB1A3GB3_backbone.seqType = "both"; sequences.seq_pSB1A3GB3_backbone.data = `LOCUS pSB1A3-GB3_backbone 5268 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_120731_229341" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
primer_bind 4048..4066
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter 632..662
/label="lac promoter"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
terminator 2004..2061
/label="his operon terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 3638..3657
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
RBS 704..715
/label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
CDS complement(3015..3875)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
terminator 1444..1515
/label="rrnB T1 terminator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(2336..2355)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
source 1..5268
/label="source"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
protein_bind 4452..4485
/label="loxP"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(2256..2844)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(2085..2102)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
promoter complement(3876..3980)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
protein_bind 670..686
/label="lac operator"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 2002..4115
/label="pSB1A3 Region"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
CDS 722..1399
/label="mRFP1"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
protein_bind 596..617
/label="CAP binding site"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 3156..3156
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 1531..1558
/label="T7Te terminator"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 4070..4113
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer complement(4088..4115)
/label="BBa_Prefix & Promoter SDM Rev"
/note="sequence: cagaaatcatccttagcgaaagctaagg"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer 4116..4135
/label="BBa_Prefix & Promoter SDM Fwd"
/note="sequence: GAATTCGCGGCCGCTTCTAGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCgaattcgaagagcctgcggc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 2002..2019
/label="pSB1A3 fwd SapI"
/note="sequence: AAAGCTCTTCAGGAagtccggcaaaaaagggc"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(4093..4115)
/label="BsaI pSB1A3 Rev"
/note="sequence: ATCTTAGGTCTCTCTCCcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer complement(4093..4115)
/label="pSB1A3 SapI rev"
/note="sequence: CCCGCTCTTCAGTGcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer 2002..2019
/label="BsaI pSB1A3 Fwd"
/note="sequence: CTCTCAGGTCTCATGCCagtccggcaaaaaagggc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(4093..4115)
/label="Linear pSB1A3 BsmBI Rev"
/note="sequence: ATTCCCCGTCTCCcagaaatcatccttagcgaaagc"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 2002..2023
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCGTTACagtccggcaaaaaagggcaagg"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 2002..2019
/label="Linear pSB1A3 BsmBI Fwd"
/note="sequence: CTATTTCGTCTCCagtccggcaaaaaagggc"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
ORIGIN
1 gaaaccgtgc ttaagagcaa gttccttgag ggggagcaca gtgccggcgt aggtgaagtc
61 gtcaatgatg tcgatatggg tcttgatcat gcacacataa ggtccgacct tatcggcaag
121 ctcaatgagc tccttggtgg tggtaacatc cagagaagca cacaggttgg ttttcttggc
181 tgccacgagc ttgagcactc gagcggcaaa ggcggacttg tggacgttag ctcgagcttc
241 gtaggagggc attttggtgg tgaagaggag actgaaataa atttagtctg cagaactttt
301 tatcggaacc ttatctgggg cagtgaagta tatgttatgg taatagttac gagttagttg
361 aacttataga tagactggac tatacggcta tcggtccaaa ttagaaagaa cgtcaatggc
421 tctctgggcg cgaattcgta taacttcgta tagcaggagt tatccgaagc gaacggtgag
481 accggatccc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
541 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
601 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
661 tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg agaaatacta
721 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
781 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
841 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
901 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
961 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
1021 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
1081 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
1141 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
1201 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
1261 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
1321 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
1381 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg
1441 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
1501 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
1561 tatatatgta cagcggtctc tgagtcatgt gtaacactcg ctctggagag ttagtcatcc
1621 gacagggtaa ctctaatctc ccaacacctt attaactctg cgtaactgta actcttcttg
1681 ccacgtcgat cttactcaat tttcctgctc atcatctgct ggattgttgt ctatcgtctg
1741 gctctaatac atttattgtt tattgcccaa acaactttca ttgcacgtaa gtgaattgtt
1801 ttataacagc gttcgccaat tgctgcgcca tcgtcgtccg gctgtcctac cgttaggggt
1861 agtgtgtctc acactaccga ggttactaga gttgggaaag cgatactgcc tcggacacac
1921 cacctgggtc ttacgactgc agagagaatc ggcgttacct ctctcacaaa gcccttcagt
1981 gcggccgctg cgtcttcctg cagtccggca aaaaagggca aggtgtcacc accctgccct
2041 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
2101 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
2161 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
2221 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
2281 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
2341 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
2401 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
2461 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
2521 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
2581 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
2641 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
2701 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
2761 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
2821 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
2881 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
2941 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
3001 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
3061 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
3121 ttaccatctg gccccagtgc tgcaatgata ccgcgcgacc cacgctcacc ggctccagat
3181 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
3241 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
3301 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
3361 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
3421 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
3481 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
3541 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
3601 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
3661 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
3721 ccgctgttga gatccagttc gatataaccc actcgtgcac ccaactgatc ttcagcatct
3781 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
3841 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
3901 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
3961 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
4021 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
4081 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgaagagcct gcggccgctg
4141 tcgggaaccg cgttcaggtg gaacaggacc acctcccttg cacttcttgg tatatcagta
4201 taggctgatg tattcatagt ggggtttttc ataataaatt tactaacggc aggcaacatt
4261 cactcggctt aaacgcaaaa cggaccgtct tgatatcttc tgacgcattg accaccgaga
4321 aatagtgtta gttaccgggt gagttattgt tcttctacac aggcgacgcc catcgtctag
4381 agttgatgta ctaactcaga tttcactacc taccctatcc ctggtacgca caaagcactt
4441 tgctagaagg tataacttcg tatagcatac attatacgaa gttattctga attccgagaa
4501 acacaacaac atgccccatt ggacagacca tgcggataca caggttgtgc agtaccatac
4561 atactcgatc agacaggtcg tctgaccatc atacaagctg aacagcgctc catacttgca
4621 cgctctctat atacacagtt aaattacata tccatagtct aacctctaac agttaatctt
4681 ctggtaagcc tcccagccag ccttctggta tcgcttggcc tcctcaatag gatctcggtt
4741 ctggccgtac agacctcggc cgacaattat gatatccgtt ccggtagaca tgacatcctc
4801 aacagttcgg tactgctgtc cgagagcgtc tcccttgtcg tcaagaccca ccccgggggt
4861 cagaataagc cagtcctcag agtcgccctt aggtcggttc tgggcaatga agccaaccac
4921 aaactcgggg tcggatcggg caagctcaat ggtctgcttg gagtactcgc cagtggccag
4981 agagcccttg caagacagct cggccagcat gagcagacct ctggccagct tctcgttggg
5041 agaggggact aggaactcct tgtactggga gttctcgtag tcagagacgt cctccttctt
5101 ctgttcagag acagtttcct cggcaccagc tcgcaggcca gcaatgattc cggttccggg
5161 tacaccgtgg gcgttggtga tatcggacca ctcggcgatt cggtgacacc ggtactggtg
5221 cttgacagtg ttgccaatat ctgcgaactt tctgtcctcg aacaggaa
//`
sequences.seq_pSB1C3_Forward_Primer = {}; sequences.seq_pSB1C3_Forward_Primer.varName = "seq_pSB1C3_Forward_Primer"; sequences.seq_pSB1C3_Forward_Primer.name = "pSB1C3 Forward Primer"; sequences.seq_pSB1C3_Forward_Primer.seqType = "linear"; sequences.seq_pSB1C3_Forward_Primer.data = `LOCUS pSB1C3_Forward_Primer 41 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TATGATCGTC TCAcaaagtc agaacagatg tttgccgatg c
//`
sequences.seq_pSB1C3_Reverse_Primer = {}; sequences.seq_pSB1C3_Reverse_Primer.varName = "seq_pSB1C3_Reverse_Primer"; sequences.seq_pSB1C3_Reverse_Primer.name = "pSB1C3 Reverse Primer"; sequences.seq_pSB1C3_Reverse_Primer.seqType = "linear"; sequences.seq_pSB1C3_Reverse_Primer.data = `LOCUS pSB1C3_Reverse_Primer 45 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACTGATCGTC TCTgttgtga agaaaaagtg aatgatgtag ccgtc
//`
sequences.seq_pSB1T3_Backbone = {}; sequences.seq_pSB1T3_Backbone.varName = "seq_pSB1T3_Backbone"; sequences.seq_pSB1T3_Backbone.name = "pSB1T3 Backbone"; sequences.seq_pSB1T3_Backbone.seqType = "both"; sequences.seq_pSB1T3_Backbone.data = `LOCUS pSB1T3_Backbone 2498 bp ds-DNA circular 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 2389..2407
/label="pBRforEco"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(120..137)
/label="L4440"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 18..38
/label="BioBrick suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 2456..2477
/label="BioBrick prefix"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
terminator 39..96
/label="his operon terminator"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
terminator 2411..2454
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind complement(371..390)
/label="pBR322ori-F"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
rep_origin complement(291..879)
/label="ori"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS 1145..2329
/label="TetR"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
ORIGIN
1 GGACTAGGTC TCATGCCtac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg
61 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
1021 agtatatatg agtaaacttg gtctgacagc tcgagattct catgtttgac agcttatcat
1081 cgataagctt taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg
1141 tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg
1201 cataggcttg gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag
1261 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
1321 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
1381 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
1441 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
1501 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
1561 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
1621 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
1681 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
1741 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
1801 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
1861 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
1921 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
1981 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
2041 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
2101 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
2161 cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc
2221 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
2281 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct aactcgagtg
2341 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
2401 acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctgaatt
2461 cgcggccgct tctagaggCG TTAGAGACCT AGAGCATT
//`
sequences.seq_pUCSTS_FWD_BspMI = {}; sequences.seq_pUCSTS_FWD_BspMI.varName = "seq_pUCSTS_FWD_BspMI"; sequences.seq_pUCSTS_FWD_BspMI.name = "pUC-STS FWD BspMI"; sequences.seq_pUCSTS_FWD_BspMI.seqType = "linear"; sequences.seq_pUCSTS_FWD_BspMI.data = `LOCUS pUC-STS_FWD_BspMI 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACAGTGGGTC TCCTAGTatg gtgtctgtga gtgag
//`
sequences.seq_pUCSTS_REV_BspMI = {}; sequences.seq_pUCSTS_REV_BspMI.varName = "seq_pUCSTS_REV_BspMI"; sequences.seq_pUCSTS_REV_BspMI.name = "pUC-STS REV BspMI"; sequences.seq_pUCSTS_REV_BspMI.seqType = "linear"; sequences.seq_pUCSTS_REV_BspMI.data = `LOCUS pUC-STS_REV_BspMI 35 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ACAGTCGGTC TCCacaatta tatggccatg ctgcg
//`
sequences.seq_pUCSTS = {}; sequences.seq_pUCSTS.varName = "seq_pUCSTS"; sequences.seq_pUCSTS.name = "pUC-STS"; sequences.seq_pUCSTS.seqType = "both"; sequences.seq_pUCSTS.data = `LOCUS pUC-STS 3622 bp ds-DNA circular 25-OCT-2020 DEFINITION . KEYWORDS "accession:addgene_35949_44214" SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
FEATURES Location/Qualifiers
CDS 1632..2492
/label="AmpR"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
misc_feature 1154..1156
/label="Threonine inside BsaI site"
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer_bind complement(1262..1281)
/label="pRS-marker"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind 3152..3171
/label="pBR322ori-F"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
source 23..1192
/label="STS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
rep_origin 2663..3251
/label="ori"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
promoter 1527..1631
/label="AmpR promoter"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter 3575..3605
/label="lac promoter"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(1850..1869)
/label="Amp-R"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer_bind complement(1441..1459)
/label="pBRforEco"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
protein_bind 3539..3560
/label="CAP binding site"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_feature 794..796
/label="Leu Codon inside BsaI Site "
/ApEinfo_revcolor=#f8d3a9
/ApEinfo_fwdcolor=#f8d3a9
primer_bind 1381..1403
/label="pGEX 3'"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
primer_bind 3405..3422
/label="L4440"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(1173..1192)
/label="STS Reverse BspMI"
/note="sequence: ACTCCTACCTGCTTCaACAAttatatggccatgctgcgga"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer complement(1193..1210)
/label="stsreverseprimer"
/note="sequence: aaataccgcagcggccgc"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer 23..44
/label="STS Forward BspMI"
/note="sequence: ACTGCTACCTGCTCCcTAGTatggtgtctgtgagtgagatcc"
/ApEinfo_revcolor=#d6b295
/ApEinfo_fwdcolor=#d6b295
primer 1..22
/label="forward primer"
/note="sequence: ctagaagaaggaggattacaaa"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 ctagaagaag gaggattaca aaatggtgtc tgtgagtgag atccgcaaag ttcaaagggc
61 agaaggccct gcaactgtat tggcgatagg cacagcaaat ccaccaaatt gtattgatca
121 gagcacatat gctgattatt attttagagt aactaacagt gaacacatga ctgatctcaa
181 gaagaagttt cagcgcattt gtgagagaac acaaatcaag aacagacata tgtacttaac
241 agaagagata ctgaaagaga atcctaacat gtgcgcatac aaagcaccgt cgttggatgc
301 aagggaagac atgatgatca gggaggtacc aagggttgga aaagaggctg caaccaaggc
361 catcaaggaa tggggtcagc caatgtctaa gatcacacat ttgatcttct gcaccaccag
421 cggtgttgca ttgcctggcg ttgattacga actcatcgta ctcttaggac tcgacccatc
481 cgtcaagagg tacatgatgt accaccaagg ctgcttcgcc ggtggcactg tccttcgttt
541 ggctaaggac ttggctgaaa acaacaagga tgctcgtgtg cttatcgttt gttctgagaa
601 taccgcagtc actttccgtg gtcctagtga gacagacatg gatagtcttg tagggcaagc
661 cttgtttgct gatggagctg ctgcgattat cattggttct gatcctgtgc cagaggttga
721 aaagcctatc tttgaaattg tttcgactga tcaaaaactt gtccctaaca gccatggagc
781 catcggtggt ctccttcgtg aagttgggct tacattctat cttaataaga gtgttcctga
841 tattatttca caaaacatca atgatgcgct cagtaaagct tttgatccat tgggtatatc
901 tgattataac tcaatatttt ggattgcaca ccctggtgga cgtgcaattt tggatcaggt
961 tgaacagaaa gtgaacttga aaccagagaa gatgaatgcc actagagacg tgcttagcaa
1021 ttacggtaac atgtcaagtg cgtgtgtgtt cttcatcatg gatttaatga ggaagaagtc
1081 ccttgaagaa ggacttaaaa ctaccggtga aggacttgat tggggcgtac tttttggctt
1141 tggtcctggt ctcactattg aaactgttgt tctccgcagc atggccatat aagcggccgc
1201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
1261 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
1321 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
1381 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
1441 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
1501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
1561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
1621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
1681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
1741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
1801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
1861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
1921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
1981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
2041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
2101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
2161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
2221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
2281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
2341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
2401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
2461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
2521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
2581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
2641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
2701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
2761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
2821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
2881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
2941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
3001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
3061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
3121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
3181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
3241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
3301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
3361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
3421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
3481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
3541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
3601 tgttgtgtgg aattgtgagc gt
//`
sequences.seq_Resveratrol_2x_Assembly = {}; sequences.seq_Resveratrol_2x_Assembly.varName = "seq_Resveratrol_2x_Assembly"; sequences.seq_Resveratrol_2x_Assembly.name = "Resveratrol 2x Assembly"; sequences.seq_Resveratrol_2x_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Assembly.data = `LOCUS Resveratrol_2x_Assembly 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
HIV-RT Binding Site join(2445..2448,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAA
//`
sequences.seq_Resveratrol_2x_Comp_Assembly = {}; sequences.seq_Resveratrol_2x_Comp_Assembly.varName = "seq_Resveratrol_2x_Comp_Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.name = "Resveratrol 2x Comp Assembly"; sequences.seq_Resveratrol_2x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_2x_Comp_Assembly.data = `LOCUS Resveratrol_2x_Comp_Ass 2448 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
HIV-RT Binding Site join(2445..2448,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2436..2444)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2425..2433)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2414..2422)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site complement(2403..2411)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2392..2400)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAA
//`
sequences.seq_Resveratrol_2x_Full_Part = {}; sequences.seq_Resveratrol_2x_Full_Part.varName = "seq_Resveratrol_2x_Full_Part"; sequences.seq_Resveratrol_2x_Full_Part.name = "Resveratrol 2x Full Part"; sequences.seq_Resveratrol_2x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_2x_Full_Part.data = `LOCUS Resveratrol_2x_Full_Par 2747 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Promoter 2632..2666
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site 2445..2554
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
HIV-RT Binding Site join(2744..2747,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2735..2743)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
misc_binding 2668..2690
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2713..2721)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Scar 2624..2631
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Terminator 2555..2623
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site complement(2724..2732)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2702..2710)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2691..2699)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
gBlock 2501..2625
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAAAA AACGTGGCGC
2461 CCGAACAGGG ACGGATAGCT CAGTCGGTAG AGCATCAGAC TTTTAATCTG AGGGTCCAGG
2521 GTTCAAGTCC CTGTTCGGGC GCCACGTTTT TTTTattagc agaaagtcaa aagcctccga
2581 ccggaggctt ttgactaaaa cttcccttgg ggttatcatt gggtactaga gTTGACGGCT
2641 AGCTCAGTCC TAGGTACAGT GCTAGCTGGA ATTGTGAGCG GATAACAATT TTTCCACACA
2701 TCGCCCACGC ATTTTCCACA CATCGCCCAC GCATGCCGTC CTCAAAA
//`
sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.name = "Resveratrol 2x r_oligo Complement Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_C 111 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature complement(42..50)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(53..61)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(64..72)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(75..83)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(86..94)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
61 CATTTTCCAC ACATCGCCCA CGCATGCCGT CCTCAAAACG AGACCAACCT G
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.name = "Resveratrol 2x r_oligo Duplex 11-33 FWD 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_1133_FWD_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 23 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCCTAGCTGG AATTGTGAGC GGA
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1 = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.varName = "seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.name = "Resveratrol 2x r_oligo Duplex 75-94 REV 1"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex_7594_REV_1.data = `LOCUS Resveratrol_2x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TTTCCACACA TCGCCCACGC
//`
sequences.seq_Resveratrol_2x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_2x_r_oligo_Duplex.varName = "seq_Resveratrol_2x_r_oligo_Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.name = "Resveratrol 2x r_oligo Duplex"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_2x_r_oligo_Duplex.data = `LOCUS Resveratrol_2x_r_oligo_D 111 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 53..61
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 64..72
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 75..83
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 86..94
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(75..94)
/label="Resveratrol 2x r_oligo Duplex 75-94 REV 1"
/note="sequence: TTTCCACACATCGCCCACGC"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 11..33
/label="Resveratrol 2x r_oligo Duplex 11-33 FWD 1"
/note="sequence: TCCTAGCTGGAATTGTGAGCGGA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAAAAACG AGACCAACCT G
//`
sequences.seq_Resveratrol_4x_Assembly = {}; sequences.seq_Resveratrol_4x_Assembly.varName = "seq_Resveratrol_4x_Assembly"; sequences.seq_Resveratrol_4x_Assembly.name = "Resveratrol 4x Assembly"; sequences.seq_Resveratrol_4x_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Assembly.data = `LOCUS Resveratrol_4x_Assembly 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 2447..2455
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2469..2477
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2458..2466
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2480..2488
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AA
//`
sequences.seq_Resveratrol_4x_Comp_Assembly = {}; sequences.seq_Resveratrol_4x_Comp_Assembly.varName = "seq_Resveratrol_4x_Comp_Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.name = "Resveratrol 4x Comp Assembly"; sequences.seq_Resveratrol_4x_Comp_Assembly.seqType = "both"; sequences.seq_Resveratrol_4x_Comp_Assembly.data = `LOCUS Resveratrol_4x_Comp_Ass 2492 bp ds-DNA circular 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Zinc Finger Site complement(2469..2477)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2392..2400)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(2458..2466)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2480..2488)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2414..2422)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site complement(2436..2444)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
HIV-RT Binding Site join(2489..2492,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2425..2433)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site complement(2403..2411)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(2447..2455)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC
2401 ATCGCCCACG CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT
2461 CCACACATCG CCCACGCATG CCGTCCTCAA AA
//`
sequences.seq_Resveratrol_4x_Full_Part = {}; sequences.seq_Resveratrol_4x_Full_Part.varName = "seq_Resveratrol_4x_Full_Part"; sequences.seq_Resveratrol_4x_Full_Part.name = "Resveratrol 4x Full Part"; sequences.seq_Resveratrol_4x_Full_Part.seqType = "both"; sequences.seq_Resveratrol_4x_Full_Part.data = `LOCUS Resveratrol_4x_Full_Par 2835 bp ds-DNA circular 14-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
Zinc Finger Site complement(2823..2831)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site complement(2768..2776)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2392..2400
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
Zinc Finger Site 2403..2411
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2480..2488
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
terminator 2265..2308
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
Scar 2668..2675
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
primer_bind complement(280..297)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Zinc Finger Site complement(2812..2820)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 2243..2261
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site 2414..2422
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 1351..1351
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
Promoter 2333..2367
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2779..2787)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 2436..2444
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer_bind complement(531..550)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
Biobricking 2311..2332
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
HIV-RT Binding Site 2489..2598
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site 2458..2466
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
HIV-RT Binding Site join(2832..2835,1..106)
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Zinc Finger Site complement(2735..2743)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_binding 2712..2734
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 2599..2667
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2425..2433
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site 2469..2477
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Backbone 197..2310
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Promoter 2676..2710
/label="J23100 Anderson Promoter"
/ApEinfo_revcolor=#d59687
/ApEinfo_fwdcolor=#d59687
Zinc Finger Site complement(2790..2798)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
gBlock 2545..2669
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
promoter complement(2071..2175)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
CDS complement(1210..2070)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2757..2765)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
Zinc Finger Site 2447..2455
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind 1833..1852
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_binding 2369..2391
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
gBlock 53..196
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Zinc Finger Site complement(2801..2809)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
rep_origin complement(451..1039)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
Zinc Finger Site complement(2746..2754)
/label="Zif268 Site"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 176..196
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Terminator 107..175
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
ORIGIN
1 AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT AGAGCATCAG ACTTTTAATC
61 TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT TTTTTTatta gcagaaagtc
121 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttgggtacta
181 gtagcggccg ctgcagagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
241 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
1021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
1081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
1141 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
1201 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
1261 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
1321 atctggcccc agtgctgcaa tgataccgcg cgacccacgc tcaccggctc cagatttatc
1381 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
1441 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
1501 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
1561 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
1621 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
1681 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
1741 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
1801 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
1861 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
1921 gttgagatcc agttcgatat aacccactcg tgcacccaac tgatcttcag catcttttac
1981 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
2041 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
2101 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
2161 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
2221 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
2281 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agTTGACGGC
2341 TAGCTCAGTC CTAGGTACAG TGCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC
2401 ATGCGTGGGC GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG
2461 TGGAAAATGC GTGGGCGATG TGTGGAAAAA AAAAAACGTG GCGCCCGAAC AGGGACGGAT
2521 AGCTCAGTCG GTAGAGCATC AGACTTTTAA TCTGAGGGTC CAGGGTTCAA GTCCCTGTTC
2581 GGGCGCCACG TTTTTTTTat tagcagaaag tcaaaagcct ccgaccggag gcttttgact
2641 aaaacttccc ttggggttat cattgggtac tagagTTGAC GGCTAGCTCA GTCCTAGGTA
2701 CAGTGCTAGC TGGAATTGTG AGCGGATAAC AATTTTTCCA CACATCGCCC ACGCATTTTC
2761 CACACATCGC CCACGCATTT TCCACACATC GCCCACGCAT TTTCCACACA TCGCCCACGC
2821 ATGCCGTCCT CAAAA
//`
sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Complement_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.name = "Resveratrol 4x r_oligo Complement Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Complement_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_C 155 bp ds-DNA linear 25-JUN-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature complement(42..50)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(53..61)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(64..72)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(75..83)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(86..94)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(97..105)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature complement(108..116)
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature complement(119..127)
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Zinc Finger Site complement(130..138)
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TTTTCCACAC ATCGCCCACG
61 CATTTTCCAC ACATCGCCCA CGCATTTTCC ACACATCGCC CACGCATTTT CCACACATCG
121 CCCACGCATG CCGTCCTCAA AACGAGACCA ACCTG
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.name = "Resveratrol 4x r_oligo Duplex 109-128 REV 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_109128_REV_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 TCGCCCACGC ATTTTCCACA
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1 = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.varName = "seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.name = "Resveratrol 4x r_oligo Duplex 39-58 FWD 1"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex_3958_FWD_1.data = `LOCUS Resveratrol_4x_r_oligo_Du 20 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 ATTGAGGACG GCATGCGTGG
//`
sequences.seq_Resveratrol_4x_r_oligo_Duplex = {}; sequences.seq_Resveratrol_4x_r_oligo_Duplex.varName = "seq_Resveratrol_4x_r_oligo_Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.name = "Resveratrol 4x r_oligo Duplex"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.seqType = "linear"; sequences.seq_Resveratrol_4x_r_oligo_Duplex.data = `LOCUS Resveratrol_4x_r_oligo_D 155 bp ds-DNA linear 07-JUL-2020 DEFINITION . FEATURES Location/Qualifiers
misc_binding 19..41
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
Zinc Finger Site 42..50
/label="ZFc Site"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 53..61
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 64..72
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 75..83
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 86..94
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 97..105
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 108..116
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 119..127
/label="Zif268"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 130..138
/label="PBSII Site"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer 39..58
/label="Resveratrol 4x r_oligo Duplex 39-58 FWD 1"
/note="sequence: ATTGAGGACGGCATGCGTGG"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
primer complement(109..128)
/label="Resveratrol 4x r_oligo Duplex 109-128 REV 1"
/note="sequence: TCGCCCACGCATTTTCCACA"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 GAACAGGGTC TCCTAGCTGG AATTGTGAGC GGATAACAAT TGAGGACGGC ATGCGTGGGC
61 GATGTGTGGA AAATGCGTGG GCGATGTGTG GAAAATGCGT GGGCGATGTG TGGAAAATGC
121 GTGGGCGATG TGTGGAAAAA AACGAGACCA ACCTG
//`
sequences.seq_r_oligo_Flipper = {}; sequences.seq_r_oligo_Flipper.varName = "seq_r_oligo_Flipper"; sequences.seq_r_oligo_Flipper.name = "r_oligo Flipper"; sequences.seq_r_oligo_Flipper.seqType = "both"; sequences.seq_r_oligo_Flipper.data = `LOCUS r_oligo_Flipper 3220 bp ds-DNA circular 02-SEP-2020 DEFINITION . FEATURES Location/Qualifiers
Biobricking 2111..2132
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
primer_bind 2043..2061
/label="pBRforEco"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
BioBrick 2964..3009
/label="BBa_B0011"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
BioBrick 2915..2955
/label="BBa_B0012"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 1151..1151
/label="Point Mutation"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
BioBrick 2915..3009
/label="BBa_B0014 (double terminator)"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
gBlock 2111..3072
/label="gBlock A"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
Scar 2956..2963
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
CDS complement(1010..1870)
/label="AmpR"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
BioBrick 2203..2908
/label="BBa_E1010 (RFP)"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
stem_loop 2976..2998
/label="stem loop"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
primer_bind 1633..1652
/label="Amp-R"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
primer_bind complement(331..350)
/label="pBR322ori-F"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
HIV-RT Binding Site 3017..3126
/label="C* HTBS"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
terminator 2065..2108
/label="bacterial terminator"
/ApEinfo_revcolor=#ffef86
/ApEinfo_fwdcolor=#ffef86
stop 2878..2883
/label="double stop codon"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
primer_bind complement(80..97)
/label="L4440"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Backbone join(3217..3220,1..2110)
/label="Mutated pSB1A3"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
gBlock 3073..3216
/label="gBlock B"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Scar 2175..2182
/label="BioBrick Scar"
/ApEinfo_revcolor=#9eafd2
/ApEinfo_fwdcolor=#9eafd2
rep_origin complement(251..839)
/label="ori"
/ApEinfo_revcolor=#b1ff67
/ApEinfo_fwdcolor=#b1ff67
rbs 2190..2194
/label="strong RBS"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
protein 2203..2908
/label="RFP"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
Terminator 3127..3195
/label="B0054"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
Biobricking 3196..3216
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
stop 2887..2892
/label="double stop codon"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
promoter complement(1871..1975)
/label="AmpR promoter"
/ApEinfo_revcolor=#faac61
/ApEinfo_fwdcolor=#faac61
start 2203..2205
/label="start codon"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
BioBrick 2183..2197
/label="BBa_B0030 (strong RBS)"
/ApEinfo_revcolor=#b4abac
/ApEinfo_fwdcolor=#b4abac
misc_feature 2183..3009
/label="From BBa_K3114015"
/ApEinfo_revcolor=#b7e6d7
/ApEinfo_fwdcolor=#b7e6d7
misc_feature 2133..2167
/label="BBa_J23105"
/ApEinfo_revcolor=#f58a5e
/ApEinfo_fwdcolor=#f58a5e
ORIGIN
1 cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc tttaaaaccg aaaagattac
61 ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
241 tgctggcgtt tttccacagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
661 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
1021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
1081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
1141 tgataccgcg cgacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
1201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
1261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
1321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
1381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
1441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
1501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
1561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
1621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
1681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatat
1741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
1801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
1861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
1921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
1981 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
2041 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
2101 atgatttctg gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacta
2161 tgctagcCGA GACCtactag agattaaaga ggagaaatac taatggcttc ctccgaagac
2221 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag
2281 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg
2341 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag
2401 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc
2461 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc
2521 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt
2581 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc
2641 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa
2701 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg
2761 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa
2821 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa
2881 taacgctgat agtgctagtg tagatcgcta ataatcacac tggctcacct tcgggtgggc
2941 ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt
3001 ttattatttG GTCTCGAAAA AAAACGTGGC GCCCGAACAG GGACGGATAG CTCAGTCGGT
3061 AGAGCATCAG ACTTTTAATC TGAGGGTCCA GGGTTCAAGT CCCTGTTCGG GCGCCACGTT
3121 TTTTTTatta gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt
3181 ggggttatca ttgggtacta gtagcggccg ctgcagagtc
//`
sequences.seq_SapI_FWD_Primer = {}; sequences.seq_SapI_FWD_Primer.varName = "seq_SapI_FWD_Primer"; sequences.seq_SapI_FWD_Primer.name = "SapI FWD Primer"; sequences.seq_SapI_FWD_Primer.seqType = "linear"; sequences.seq_SapI_FWD_Primer.data = `LOCUS SapI_FWD_Primer 32 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 AAAGCTCTTC AGGAagtccg gcaaaaaagg gc
//`
sequences.seq_SapI_Rev_Primer = {}; sequences.seq_SapI_Rev_Primer.varName = "seq_SapI_Rev_Primer"; sequences.seq_SapI_Rev_Primer.name = "SapI Rev Primer"; sequences.seq_SapI_Rev_Primer.seqType = "linear"; sequences.seq_SapI_Rev_Primer.data = `LOCUS SapI_Rev_Primer 37 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers ORIGIN
1 CCCGCTCTTC AGTGcagaaa tcatccttag cgaaagc
//`
sequences.seq_STS_intro_gblock = {}; sequences.seq_STS_intro_gblock.varName = "seq_STS_intro_gblock"; sequences.seq_STS_intro_gblock.name = "STS intro gblock"; sequences.seq_STS_intro_gblock.seqType = "linear"; sequences.seq_STS_intro_gblock.data = `LOCUS STS_intro_gblock 474 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 40..74
/label="BBa_J23111"
/ApEinfo_revcolor=#c6c9d1
/ApEinfo_fwdcolor=#c6c9d1
misc_feature 123..431
/label="PBSII"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
misc_feature 99..112
/label="BBa_J61101"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
Biobricking 18..39
/label="BioBrick Prefix "
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_binding 75..98
/label="LacO"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
misc_feature 432..461
/label="Spacer"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer 18..41
/label="forward Prefix-Promoter-PBS2-sapI primer"
/note="sequence: AAAGCTCTTCACACgaattcgcggccgcttctagagtt"
/ApEinfo_revcolor=#85dae9
/ApEinfo_fwdcolor=#85dae9
primer complement(439..461)
/label="reverse Prefix-Promoter-PBS2-sapI primer"
/note="sequence: AAAGCTCTTCAACTactacctccacttccacctccac"
/ApEinfo_revcolor=#c7b0e3
/ApEinfo_fwdcolor=#c7b0e3
ORIGIN
1 ACCATCGGTC TCACGTTgaa ttcgcggccg cttctagagt tgacggctag ctcagtccta
61 ggtatagtgc tagcTGGAAT TGTGAGCGGA TAACAATTGA AAGACAGGAC CCtaaataca
121 tgATGCATCA CCATCACCAT CATCCAGGCG AGAAACCGTA CGCCTGCCCC GAATGCGGTA
181 AATCATTTAG TCAGCGGGCG AACTTACGCG CACACCAGCG AACCCATACC GGAGAAAAAC
241 CGTATAAATG TCCGGAATGT GGTAAAAGCT TTAGCCGTTC GGATCATCTG ACGACTCACC
301 AACGCACACA CACCGGGGAA AAGCCGTATA AATGCCCTGA GTGTGGTAAG TCGTTCTCCC
361 GTTCTGACGT TCTGGTGCGC CATCAGCGTA CGCATACTGG TGGCGGGAGC GGCGGCGGAA
421 GTGGTGGCTC Cggtggaagt ggaggtggaa gtggaggtag tAGAGACCTC CTTT
//`
sequences.seq_STS_outro_gblock = {}; sequences.seq_STS_outro_gblock.varName = "seq_STS_outro_gblock"; sequences.seq_STS_outro_gblock.name = "STS outro gblock"; sequences.seq_STS_outro_gblock.seqType = "linear"; sequences.seq_STS_outro_gblock.data = `LOCUS STS_outro_gblock 129 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
stem_loop 27..78
/label="T0 terminator"
/ApEinfo_revcolor=#75c6a9
/ApEinfo_fwdcolor=#75c6a9
Biobricking 79..99
/label="BioBrick Suffix"
/ApEinfo_revcolor=#84b0dc
/ApEinfo_fwdcolor=#84b0dc
ORIGIN
1 gtcaagctgc catgacgagG GTCTCAttgt tcagaacgct cggtcttgca caccgggcgt
61 tttttctttg tgagtccata ctagtagcgg ccgctgcagT GCCAGAGACC tccaagagag
121 tgaagggat
//`
sequences.seq_ZFc_codon_optimized_1 = {}; sequences.seq_ZFc_codon_optimized_1.varName = "seq_ZFc_codon_optimized_1"; sequences.seq_ZFc_codon_optimized_1.name = "ZFc (codon optimized) (1)"; sequences.seq_ZFc_codon_optimized_1.seqType = "linear"; sequences.seq_ZFc_codon_optimized_1.data = `LOCUS ZFc_(codon_optimized) 331 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 18..311
/label="ZFc with linker"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
ORIGIN
1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA
61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG
121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA
181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA
241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC
301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T
//`
sequences.seq_ZFc_codon_optimized = {}; sequences.seq_ZFc_codon_optimized.varName = "seq_ZFc_codon_optimized"; sequences.seq_ZFc_codon_optimized.name = "ZFc (codon optimized)"; sequences.seq_ZFc_codon_optimized.seqType = "linear"; sequences.seq_ZFc_codon_optimized.data = `LOCUS ZFc_(codon_optimized) 331 bp ds-DNA linear 25-OCT-2020 DEFINITION . FEATURES Location/Qualifiers
misc_feature 18..311
/label="ZFc with linker"
/ApEinfo_revcolor=#ff9ccd
/ApEinfo_fwdcolor=#ff9ccd
ORIGIN
1 TGACTAGGTC TCATAGCACG TCAGCAGCGG CGCCGGGGGA ACGACCGTTT CAGTGCCGCA
61 TTTGTATGCG TAACTTCTCC GATAGTCCAA CCCTGAGACG CCATACTCGC ACCCACACCG
121 GTGAAAAGCC GTTCCAATGC CGCATTTGTA TGCGTAACTT TTCGGTTCGC CACAATCTTA
181 CACGTCACTT ACGTACGCAT ACCGGCGAGA AACCCTTCCA GTGCCGTATC TGTATGCGCA
241 ATTTTAGCGA TCGGACGTCT TTGGCCCGGC ATCTGAAAAC TCATTATCCT TACGATGTGC
301 CGGACTATGC TTAAGAGTAG AGACCTGCGA T
//`