Presented by iGEM_20 QHFZ-China
Authors:
Poster design: Bohan Zhang1, 2
Wiki code: Xinyuan Wang1, 2
Content: Yixian Yang1, 2
Proofread: Celine Siu Ham Zhang1, 2
Instructor: Xing (Leaves) Zhang1, 3
1. iGEM team QHFZ-China 2020, China, Asia.
2. Tsinghua University High School, Beijing, China.
3. Beijing ZENO Co., Ltd., Beijing, China.
Abstract
Various engineered bacteria showed useful functions. However, the storage of these bacteria usually requires -80℃ refrigerators, which substantially limited their transportation and usages in daily life. Therefore, we decided to utilize TDPs from tardigrades (water bears) to supply a new storage method. By introducing the TDPs as the protectants into bacteria during lyophilization (freeze-drying), we could produce dry live-bacteria powder. The powder then can be stored at room temperature for a long time, without any usage of professional equipment. This year, we confirmed that certain TDPs have the ability to maintain the survival rate of E. coli. We also optimized the method by regulating the expression level and combining different TDPs. In addition, we proved the modularity and tried to regulate the degradation of TDPs after freeze-drying. Considering that many iGEM teams and reseachers are designing engineered bacteria for various application scenarios out of laboratory, we hope the improved biopreservation method would promote their practical application.
Team
This is the second year for QHFZ-China to attend iGEM family. This year, QHFZ-China is composed of 15 students (Leaders: Yixian Yang, Zhandong Jiao; Members: Celine Siu Ham Zhang, Jiangshan Gao, Sizhe Duan, Marco Xue, Yeqi Wong, Xinyuan Wang, Bert Shan, James Haorui Wang, Junfei (Sophie) Li, Yiming Tang, Shao Chang, Siyuan Wang, Bohan Zhang), 6 advisors (Zhimeng Xu, Jun Ma, Aiqi Zhao, Li TianHong, Luo Shaowei, Weizhao Chen), 2 instructors (Xing (Leaves) Zhang, Tianze Zhu) and a primary PI (Shiqi Wang). All of us welcome your visiting our poster!
