Team:Fudan/Poster

Poster: 2020 Fudan

Make calcium supplement sustainable

Bone appetite
  • Student Leader: Shizheng Tian
  • Student Members: Shuojin Liang, Mingwei Li, Ziyuan Wei, Jingqi Zhang, Tianhui Xu, Zhenru Zhang, Yiyang Yao, Yiyang Lu, Yuanyuan Liu, Emily Mo, Xiaohui Jiang, Xinran Li, Gaochen Zhang
  • Advisors: Liang Cai, Yijie Pan, Yuchang Lee, Jingyi Hu
  • Team PI: Daru Lu
  • Affiliation: School of Life Sciences, Fudan University; with funding from YF Capital

In Asia, many old people suffer from insufficient calcium intake and related diseases such as osteoporosis. Considering that traditional calcium supplementation is not efficient and user-friendly enough, we intended to develop a kind of engineered bacteria based on E. coli that can colonize in the human intestine and secrete peptides to promote calcium absorption.

We introduced the quorum sensing system to colonize bacteria in the intestine. Once the bacteria have successfully colonized the intestinal tract, the luxPR promoter will be activated and a series of short peptides that can bind to calcium and promote calcium absorption of intestinal epithelial cells will be expressed. A kill switch is added accordingly for safety.

Our project provides a novel delivery method to provide calcium supplements sustainably. And we are actively seeking commercialization possibilities to expand our project impact, to reach more population, for the elderly's good health and well-being.

So far, what we have achieved

项目缘起，需要HP那四张关于骨质疏松的问卷图，数据不变 重画

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铜奖满足，但不是罗列因为评委有一个列表给奖牌打分

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银奖满足，但不是罗列因为评委有一个列表给奖牌打分

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金奖满足，但不是罗列因为评委有一个列表给奖牌打分

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总结的话

SDG 3: GOOD HEALTH AND WELL-BEING

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SDG 4: QUALITY EDUCATION

描述我们项目如何贡献SDG4；我们为什么要教育，完成了什么，后来人继续做什么 bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la

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SDG 9: INDUSTRY, INNOVATION AND INFRASTRUCTURE

描述我们项目如何贡献SDG9；我们完成了什么，下一步将要做什么 bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la

bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la bala bala bala ba la ba la

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We invented CaAP

CaAP stands for Calcium Absorption Peptide. According to literature, we selected peptides @@@@GPAGPHGPVG, FDHIVY, YQEPVIAPKL, NDEELNK, and DHTKE. All of the first four peptides can form calcium-peptide complex through their Asp and Glu residues, and facilitate cell uptake. DHTKE uses its Asp, Glu and His residues to do the same. Basic part @@@@ We add a signal peptide to the N segment of CaAP to guide its secretion into the periplasmic space of E. coli. Because it is so small, it will then leak out of the cell wall into the outside environment. @@@@ 2 existing parts, 3 new; composite parts We linked these peptides through Phe-Arg (FR) junctions. When CaAP is expressed and secreted by the engineered E. coli in the intestinal environment, CaAP could be further cleaved into functional oligopeptides in the presence of digestive enzymes (trypsin and chymotrypsin) in the intestinal lumen.

We improved mcbABCD
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CaAsst for measuring trace calcium ion
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How to use our parts
What did your team achieve? What do you plan to work on moving forward?
Acknowledgements
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