Team:XMU-China/Poster

Antea-Glyphosate

Presented by Team XMU-China 2020

Jinzhu Mao¹, Shi Zhang¹, Siru Zhou¹, Meihao Ou¹, Qingliu Wang¹, Ruijie Mo¹, Ruomeng Bi¹, Shichen Geng¹, Shuling Xiao¹, Xiaoyu Wang¹, Yangqi Deng¹, YiXian Zheng¹, Zhichun Yang¹, Shengyang Zhang¹, Jisheng Xie¹, Yuan Li¹, Gezhi Xiao², Xiao-yan Zhuang², Ai-hui Zhang², Wang Yali², Fu yousi², Junhong Chen², Zinuo Huang², Yang Liang², Xingyu Chen², YanSong Chen², Fude Chen², Shiyi Zhai², Langxing Liao², Limin Qiu², Tianyu Feng², Wenyao Shao², Yajuan Peng², Haitao Wang², Yang Shi², Jiacheng Huang², Baishan Fang³, Xiamen University, Xiamen, Fujian, China

Attributions

Brainstorms: All team members.

Molecular cloning and circuit construction: All team members.

Characterization: All team members.

Proof of Concept: All team members.

Model: Yixian Zheng, Siru Zhou, Ruomeng Bi, Ruijie Mo, Jinzhu Mao.

Human practices: All team members.

Wiki: Shengyang Zhang.

Art design: Yangqi Deng, Fude Chen, Jisheng Xie, Junhong Chen.

!!!这里他妈的缺一张图

Abstract

Tea is deeply rooted in Chinese culture. For a long period, a large amount of glyphosate has been used as a herbicide, which raises a severe problem of pesticide residues in tea and tea products.
!!!这里他妈的缺一张图
This year XMU-China aims at developing an efficient glyphosate detection and degradation system.

There are two pathways found to degrade glyphosate:
►Glyphosate generates AMPA and glyoxylic acid through the C-N cleavage.
The product, glyoxylic acid, could be further converted into measurable signal, thus for the detection system, glyphosate is degraded by several enzymes through C-N cleavage and then transformed into a measurable fluorescence signal caused by the NADPH.
►Glyphosate generates sarcosine and phosphate acid through the C-P cleavage.
The product by C-P cleavage pathway, sarcosine, could be rapidly oxidized to become formaldehyde, a non-toxic substance. Therefore, glyphosate degradation method through C-P cleavage is preferred in our design which plans to disintegrate glyphosate to minimize the toxicity.
!!!这里他妈的缺一张图
►Two kill switches controlled by different inducers are also proposed.
!!!这里他妈的缺一张图
It is hoped that this project could provide new ideas for the detection and degradation of pesticide residues. Taking care of the earth by tiny bacteria, we here promise a better future of tea.
!!!这里他妈的缺一张图