The goal of our project was to design and construct a biosensor for macrolide detection and quantification.
- Our basic parts include: ermC, mphr, pMphR, egfp and RBS associated with pMphR
- Our composite parts include: Biosensor for erythromycin detection
ermC is a methyltransferase, which introduces dimethylation of the adenine residue in 23S rRNA at position 2085, thus preventing binding of macrolide and lincosamide antibiotics .
A mphr gene encoding a repressor protein binding pMphR promoter sequence preventing the transcriptions of genes under it .
A pMphR promoter sequence, which naturally drives transcription of mphr, a macrolide antibiotics resistance gene .
EGFP is an enhanced green fluorescence protein .
RBS associated with pMphR naturally allows translation of the Mph(A) gene .
Biosensor for Erythromycin Detection: BBa_K3386004
Our composite part is a biosensor device for erythromycin detection.
SUMMARY OF PARTS
1. UniProt: a worldwide hub of protein knowledge. (2018). Nucleic Acids Research, 47(D1), D506-D515. doi:
2. Noguchi, N., Takada, K., Katayama, J., Emura, A., & Sasatsu, M. (2000). Regulation of Transcription of themph(A) Gene for Macrolide 2′-Phosphotransferase I inEscherichia coli: Characterization of the Regulatory Gene mphR(A). Journal Of Bacteriology, 182(18), 5052-5058. doi: 10.1128/jb.182.18.5052-5058.2000
3. Gardner, L., Zou, Y., Mara, A., Cropp, T., & Deiters, A. (2011). Photochemical control of bacterial signal processing using a light-activated erythromycin. Molecular Biosystems, 7(9), 2554. doi: 10.1039/c1mb05166k
4. Te-Tuan Yang, Linzhao Cheng, Steven R. Kain, Optimized Codon Usage and Chromophore Mutations Provide Enhanced Sensitivity with the Green Fluorescent Protein, Nucleic Acids Research, Volume 24, Issue 22, 1 November 1996, Pages 4592–4593, https://doi.org/10.1093/nar/24.22.4592