Team:Exeter/Notebook/August27
Thursday, 27 August
Lab Work
We began transformation of B. subtilis using the 18 plasmid constructs cloned on the 17th.
In the morning Bethan and Matthew made up two media (Medium A to sustain bacterial growth and Medium B to encourage competency), then incubated B. sub in Medium A in the shaking incubator at 37C, measuring it in the spectrophotometer every 20 minutes until growth had levelled off. We left it to incubate for a further 90 minutes.
Afterwards, we placed the B. sub in Medium B in tubes labelled 1-18 (for transformation) and 19 (control), then placed it in the shaking incubator again for 90 minutes.
In the afternoon Adam & Eloise pipetted the 18 plasmid constructs we had cloned on the 17th of August into the tubes. We then plated the B sub onto agar plates laced with antibiotic that would kill bacteria which had not taken up the plasmid.
The full protocol can be downloaded here.
Team Collaborations
UNSW got back to us saying they would be happy to have a virtual meeting with us. We suggested the 28th of August but this date didn't work for them and we arranged a different meeting date in later communications.