Team:Exeter/Notebook/Oct19

Notebook (Exeter iGEM 2020)

Monday, 19 October

Chloe emailed Prof. James Wakefield (head of biosciences) to ask if we could book a seminar room with high-quality recording equipment next Friday to record our final presentation.

Lab Work

When we did the agarose experiments to determine the rate which CO2 would diffuse through hydrogel, we left a camera on overnight each time to record the colour change. Today Mark uploaded the images (about 1000 of them per experiment!) to Teams.

Hydrogels and Modelling

Mark and Velizar worked on coding a preliminary program in MATLAB which could analyse the colour change in the hydrogel pictures over time by recording the ratio of yellow to red pixels:

graph which shows a gradual increase then a dip then a steep increase

Team Collaborations

We sent UCL an address to send their biobrick DNA to so we could do some wet lab work with it.

Matthew also spoke to them to find out some more specific information about the DNA sequence they were sending us - namely that it is a circular plasmid containing a full BioBrick and an antibiotic resistance gene for Chloramphinecol. This is good news because the DNA is in a format that we will be able to transform straight into E. coli, so we can complete the transformation before wiki freeze.

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