Team:GreatBay SZ/Parts
Basic Parts
Pilin Expression
Electrically conductive protein nanowire (e-PN) is the most fundamental and essential part of our project, which can generate the electrical power by utilizing moisture in the air. This kind of protein can be produced by Geobacter sulfurreducens, but it is an anaerobic bacteria which needs to be cultivated under strict conditions. Therefore, we decide only to use the pilA gene from Geobacter sulfurreducens and build the genetic pathway by ourselves.
The expression of e-PN in E. coli Top10 involves two parts: one part is the monomeric protein gene pilA(pilin gene) of e-PN, and the other part is the e-PN assembly system called TypeVI system, which comes from E. coli O157: H7 and MG1655.
| Part Name | Part Code |
|---|---|
| PpdD Signal | BBa_K3596000 |
| Pilin Gene | BBa_K3596001 |
| hofB RBS | BBa_K3596002 |
| hofB+hofC | BBa_K3596003 |
| hofM RBS | BBa_K3596004 |
| hofM+hofN+hofO+hofP+hofQ | BBa_K3596005 |
| ppdA RBS | BBa_K3596006 |
| ppdA+ppdB+ygdB+ppdC+gspO | BBa_K3596007 |
| fimA knock out gRNA1 | BBa_K3596053 |
| fimA knock out gRNA2 | BBa_K3596054 |
| PpdD Signal+Pilin gene | BBa_K3596055 |
RBS Mutation
We enhance the productivity of the protein nanowire by creating library of RBS hofB, hofM and ppdA in the Type IV pilin assembly system, so as to find the optimized combination of the three RBSs with varied strengthes.
| Part Name | Part Code |
|---|---|
| ppdA RBS mut1 | BBa_K3596008 |
| ppdA RBS mut2 | BBa_K3596009 |
| hofB RBS mut1 | BBa_K3596010 |
| hofB RBS mut2 | BBa_K3596011 |
| hofM RBS mut | BBa_K3596012 |
Pilin Gene Mutation
We design 17 mutations of pilin gene for the purpose of adding more carboxylic groups into the nanowires and decreasing the diameter of them, so as to improve the output voltage of the protein film.
| Part Name | Part Code |
|---|---|
| Pilin Gene mut 1A | BBa_K3596013 |
| Pilin Gene mut 1B | BBa_K3596014 |
| Pilin Gene mut 1C | BBa_K3596015 |
| Pilin Gene mut 1D | BBa_K3596016 |
| Pilin Gene mut 2A | BBa_K3596017 |
| Pilin Gene mut 2B | BBa_K3596018 |
| Pilin Gene mut 2C | BBa_K3596019 |
| Pilin Gene mut 2D | BBa_K3596020 |
| Pilin Gene mut 3A | BBa_K3596021 |
| Pilin Gene mut 3B | BBa_K3596022 |
| Pilin Gene mut 3C | BBa_K3596023 |
| Pilin Gene mut 3D | BBa_K3596024 |
| Pilin Gene mut 4A | BBa_K3596025 |
| Pilin Gene mut 4B | BBa_K3596026 |
| Pilin Gene mut 4C | BBa_K3596027 |
| Pilin Gene mut 4D | BBa_K3596028 |
| Pilin Gene mut thin | BBa_K3596029 |
Composite Parts
| Part Name | Part Code |
|---|---|
| BIOT: PpdD Signal+Pilin Gene+ TypeIV pilin assembly system | BBa_K3596030 |
| BIOT mut 1A | BBa_K3596031 |
| BIOT mut 1B | BBa_K3596032 |
| BIOT mut 1C | BBa_K3596033 |
| BIOT mut 1D | BBa_K3596034 |
| BIOT mut 2A | BBa_K3596035 |
| BIOT mut 2B | BBa_K3596036 |
| BIOT mut 2C | BBa_K3596037 |
| BIOT mut 2D | BBa_K3596038 |
| BIOT mut 3A | BBa_K3596039 |
| BIOT mut 3B | BBa_K3596040 |
| BIOT mut 3C | BBa_K3596041 |
| BIOT mut 3D | BBa_K3596042 |
| BIOT mut 4A | BBa_K3596043 |
| BIOT mut 4B | BBa_K3596044 |
| BIOT mut 4C | BBa_K3596045 |
| BIOT mut 4D | BBa_K3596046 |
| BIOT mut thin | BBa_K3596047 |
| BIOT RBS mut1 | BBa_K3596048 |
| BIOT RBS mut2 | BBa_K3596049 |
| BIOT RBS mut3 | BBa_K3596050 | BIOT RBS mut4 | BBa_K3596051 |
| BIOT RBS mut5 | BBa_K3596052 |
| BIOT with RBS mut4 and Pilin gene mut4A | BBa_K3596056 |