Team:NCTU Formosa/Criteria
1. Integrated human practices
Our integrated human practice includes three target groups: At the professor part, We know that the increasing intracellular ATP may be useful in pathways and especially under specific conditions. Therefore, we put it under low nutrient conditions to test its function. Professor Koa's suggestion solves the problem of measuring the absorbance of our Lemo21 with beta-carotene producing plasmid. In public, we have held many conferences and made a Carbon Resource Consumption Survey. We found that the public takes environmental problems seriously and improves the device to work outside and eco-friendly.
Last but not least, we have visited Grape King Bio and TWBio. The E. Hybrid can be applied to medicine manufacturing and ARK story. We also further calculate the cell cycle in the model and, more accurately, reconfirm our results. Finally, we have done several SDGs in Hsinchu. Integrating all these feedback and advice, we benefit from every phase and gradually shape the project.
2. Project modeling
For the project’s design, we simulate our protein to determine the complex transmembrane structure. Moreover, analyzing the functional test to learn more about the expression and function of gloeobacter rhodopsin. Our model predicts the results before the experiment, and it is exact in describing the difference between E. Hybrid and wild type E. coli. We also analyze the change of growth with different glucose concentrations. Furthermore, the model can optimize the growth and production to expand the benefit of E. Hybrid. We also improve the device’s setting like refresh strategy and predict the end time to use E. Hybrid conveniently.
3. Proof of concept
After confirming the function of GR, we tested the expression of the target protein, RFP. The Model also predicts and numerically confirms that the experimental results of both growth of E. Hybrid and expression of RFP are better than E. coli without GR expressing system. From the experiment, we proved that E. Hybrid could incorporate with different target proteins for various applications. We also use devices to demonstrate the production of E. Hybrid and detect the process. Custom regulation of devices and calculated results of models also express the concept of better growth and production in the application.
4. Partnership
This year we have made a partnership with our neighbor, NTHU_Taiwan. We have collaborated with them in many aspects, both of us witness the development of our projects. At the beginning of our partnership, we have an online meetup to share our future prospects to roughly introduce project design, we get to make friends with each other and ready for the endless but meaningful trip of iGEM 2020.
5. Science communication
Apart from completing our project, being able to communicate with other groups is also critical even though there are so many hindrances in this year. We have held NCTU X NTHU conference, high school workshop, NCTU Presentation and participated in Taiwan iGEM conference, and other online communication, social network and online meetup. In addition, we record content about our project and iGEM with go-to and play, which is a radio channel in NCTU. After doing these science communication, we do stretch our idea out and also take back some valuable advice.
1. Engineering Success
We followed the engineering design cycle from research to improve. Beginning with our research of GR, we designed the experiment to acknowledge whether E.Hybrid can grow faster than wild type E.coli. As for models, we are able to determine
the growth rate and analyze the growth curve of E. Hybrid, and it also grows much faster as our expectations.
2. Collaboration
We have collaborated with other teams in many aspects to fulfill our project, experiment, hardware, survey, social media and SDGs are included.
3. Human Practices
As the global warming and atrocious weather continuously raged on our planet, we epitomized the value of synthetic biology in aggressively solving this problem. On top of that, we take actions to spread this value to publics like conferences
and online meetups. Visite our Human practice page and you will get more details.
4. Proposed Implementation
According to our value of project, we recognized our target customers and research our competition position in the market.
1. Competition Deliverables
We have completed all the Competition Deliverables, including wiki, poster, presentation video, project promotion video and judging form.
2. Attributions
To show our thankfulness to every person that made our project happen, visit the Attributions page.
3. Project Description
Check out how E. Hybrid was born in our Description page.
4. Contribution
We add new parts, Gloeobacter rhodopsin in junction with GFP. We also create periplasmic pumps.