BBa_K3526000 is a basic part, a special transcription factor AtzR. This part should be used with the part the basic part BBa_K3526003. Under the presence of cyanuric acid (CYA), the AtzR can bind CYA and regulate the promoter PatzD. As a result, the GFP is expressed and emit green fluorescence.
To test the function of part BBa_K3526000 and BBa_K3526003, we build two composite part BBa_K3526004 and BBa_K3526005 into Pseudomonas putida KT2440. After reading related articles, we choose 0μM, 50μM, and 100μM as the detection concentrations of CYA. After preliminary analysis, we obtained Figure 1.
The results of the experiment show that with the presence of part BBa_K3526004 and BBa_K3526005, pseudomonas putida KT2440 can detect cyanuric acid.
Wechat: Search "AtreXtinct" in WeChat