Difference between revisions of "Team:BITSPilani-Goa India"

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<html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>SugarGain | iGEM BITS Goa</title><link href="https://2020.igem.org/Template:BITSPilani-Goa_India/css/indexCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><nav id="main-nav"><div class="navbar bg-none nav-transparent"><div class="row" id="navbar-row"><a class="navbar-brand" href="https://2020.igem.org/Team:BITSPilani-Goa_India"><i class="navbar-logo"></i><span> Sugar<strong>Gain</strong></span></a><div class="controls h-100 d-flex align-items-center"><div class="align-items-center" id="themeSwitchWrapper"><i class="icon-light"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="icon-dark"></i></div><button class="btn" id="menuSwitch"><div class="menu-icon"></div></button></div></div><div class="row w-100" id="progress-container-wrapper"><div id="progress-container"><div id="nav-progress-bar"></div></div></div></div><div id="fullscreenMenuWrapper"><div class="w-100 h-100" id="fullscreenMenu"><div class="container-fluid d-flex align-items-stretch pr-0 flex-column"><div class="row nav-header w-100"><div class="col-xl-3 col-lg-2"><div class="teamLogo"></div></div><div class="col-xl-6 col-lg-8"></div><div class="col-xl-3 col-lg-2"></div></div><div class="row flex-grow-1 w-100"><div class="col-xl-3 col-lg-2"></div><div class="col-xl-6 col-lg-8 d-flex justify-content-between flex-column" id="menuContent"><div class="row"><div class="col-md-6" id="nav-headings"><ul role="tablist"><li><a href="javascript:;" id="Project-tab">Project</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Description">Description</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Design">Design</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Model">Model</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Engineering">Engineering</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Safety">Safety</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Implementation">Implementation</a></li></ul></li><li><a href="javascript:;" id="Human_Practices-tab">Human Practices</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Human_Practices">Human-Centered Design</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Education">Science Communication</a></li></ul></li><li><a href="javascript:;" id="Parts-tab">Parts</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Parts">Parts</a></li></ul></li><li><a href="javascript:;" id="Journal-tab">Journal</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Notebook">Notebook</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments">Experiments</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Appendix">Appendix</a></li></ul></li><li><a href="javascript:;" id="Team-tab">Team</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Team">Team</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Attributions">Attributions</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Collaborations">Collaborations</a></li></ul></li><li><a href="javascript:;" id="Contribution-tab">Contribution</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution">Overview</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Software">Software</a></li></ul></li><li><a href="javascript:;" id="Entrepreneurship-tab">Entrepreneurship</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Entrepreneurship">Entrepreneurship</a></li></ul></li><li><a href="javascript:;" id="Judging-tab">Judging</a><ul class="mobile-nav-items"><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Judging">Judging</a></li></ul></li></ul></div><div class="col-6" id="nav-items"><div class="tab-content"><div class="tab-pane" id="Project-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Description">Description</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Design">Design</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Model">Model</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Engineering">Engineering</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Safety">Safety</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Implementation">Implementation</a></li></ul></div><div class="tab-pane" id="Human_Practices-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Human_Practices">Human-Centered Design</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Education">Science Communication</a></li></ul></div><div class="tab-pane" id="Parts-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Parts">Parts</a></li></ul></div><div class="tab-pane" id="Journal-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Notebook">Notebook</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments">Experiments</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Appendix">Appendix</a></li></ul></div><div class="tab-pane" id="Team-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Team">Team</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Attributions">Attributions</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Collaborations">Collaborations</a></li></ul></div><div class="tab-pane" id="Contribution-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution">Overview</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Software">Software</a></li></ul></div><div class="tab-pane" id="Entrepreneurship-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Entrepreneurship">Entrepreneurship</a></li></ul></div><div class="tab-pane" id="Judging-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Judging">Judging</a></li></ul></div></div></div></div><div class="row"><div id="contact-links"><ul><li><a href="https://instagram.com/igem_bits" rel="noopener" target="_blank"><i class="insta"></i><span>Instagram</span></a></li><li><a href="https://twitter.com/igembitsgoa" rel="noopener" target="_blank"><i class="twitter"></i><span>Twitter</span></a></li><li><a href="mailto:igembitsgoa@gmail.com" rel="noopener" target="_blank"><i class="email"></i><span>Email</span></a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Sample"><i class="sample"></i><span>Sample</span></a></li></ul><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Acknowledgements" rel="noopener" target="_blank">Acknowledgements</a></li></ul></div></div></div><div class="col-xl-3 col-lg-2"></div></div><div class="row nav-footer w-100"><div class="col-xl-3 col-lg-2"></div><div class="col-xl-6 col-lg-8"></div><div class="col-xl-3 col-lg-2"></div></div></div></div></div></nav><header class="d-flex justify-content-center align-items-center"><video autoplay="true" loop="" muted=""><source src="https://static.igem.org/mediawiki/2020/d/db/T--BITSPilani-Goa_India--video--homepage.mp4" type="video/mp4"/></video></header><section class="section1" id="description"><div class="container"><div class="row"><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/4/43/T--BITSPilani-Goa_India--img--Home--Description.svg"/><h3>A cold winter day —<br/>The bacteria inhibit<br/>Invertase from acting.</h3></div><div class="content col-lg-9"><h2>Tonnes of sugar are lost every year as post-harvest losses. <span>We're here to change that.</span></h2><p></p><p>Sugarcane is one of India’s most important crops. It is cultivated on 5 million hectares of land, making India the second-largest producer of the crop which contributes to 6% of the national GDP and forms the livelihood for 20 million people across the country.</p><p></p><p>This industry is in peril, however. Post-harvest sucrose deterioration is responsible for losses worth millions of rupees. Following its harvest, enzymatic activity reduces sugar recovery rates by upto 10%!</p><p>Our solution mitigates this problem.<br/>We’re SugarGain, and we’re making the world a sweeter place, quite literally!</p><div class="row mt-5"><div class="col-sm-5"><h3>A cold winter day —<br/>The bacteria inhibit<br/>Invertase from acting.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/4/43/T--BITSPilani-Goa_India--img--Home--Description.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Description"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Description</span></a></div></div></div></div></section><section class="section2" id="design"><div class="container"><div class="row"><div class="content col-lg-9"><h2>Design caption goes here.</h2><p>We aim to target invertase present in the sugarcane stem - the enzyme responsible for cleaving sucrose into its constituents. Our project is structured into three major modules: a fructose regulated anti-invertase construct, an atmospherically-regulated kill switch, and a novel polymer inoculant.</p><div class="row mt-5"><div class="col-sm-5"><h3>Reacting to fructose<br/>The bacteria synthesise<br/>Anti-invertase.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/8/87/T--BITSPilani-Goa_India--img--Home--Design.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Design"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Design</span></a></div></div><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/8/87/T--BITSPilani-Goa_India--img--Home--Design.svg"/><h3>Reacting to fructose<br/>The bacteria synthesise<br/>Anti-invertase.</h3></div></div></div></section><section class="section3" id="safety"><div class="container"><div class="row"><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/c/c4/T--BITSPilani-Goa_India--img--Home--Safety.svg"/><h3>The microbe escapes<br/>its confined space. But Alas!<br/>The kill switch goes off.</h3></div><div class="content col-lg-9"><h2>Safety caption goes here.</h2><p>Biosafety is a cruicial aspect of our project, especially so when our chassis is so closely involved with food products.</p><p>Throughout our project design we have tried to be cognizant about identifying strategies for robust biosafety. To ensure the highest possible levels of safety, we have developed a three-tier failsafe meachanism after taking into consideration the various use cases of our chassis.</p><div class="row mt-5"><div class="col-sm-5"><h3>The microbe escapes<br/>its confined space. But Alas!<br/>The kill switch goes off.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/c/c4/T--BITSPilani-Goa_India--img--Home--Safety.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Safety"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Safety</span></a></div></div></div></div></section><section class="section4" id="human-practices"><div class="container"><div class="row"><div class="content col-lg-9"><h2>Human-centered design.</h2><p>Human Practices is essential for responsible project design. Our approach to human practices consisted of multiple iterations which allowed us to effectively interview industry experts, researchers and users. Throughout our project, we identified and spoke to various diverse stakeholders relevant to our project.</p><p>We have tried our best to inculcate this feedback at every stage of design of our project.</p><div class="row mt-5"><div class="col-sm-5"><h3>Science and humans,<br/>Like shadow to a body:<br/>Inseparable.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/3/31/T--BITSPilani-Goa_India--img--Home--Human-Practices.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Human_Practices"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Human Practices</span></a></div></div><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/3/31/T--BITSPilani-Goa_India--img--Home--Human-Practices.svg"/><h3>Science and humans,<br/>Like shadow to a body:<br/>Inseparable.</h3></div></div></div></section><section class="section5" id="collaboration"><div class="container"><div class="row"><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--img--Home--Modelling.svg"/><h3>Helping us with issues,<br/>And with making new friends too! —<br/>Collaborations.</h3></div><div class="content col-lg-9"><h2>Modelling caption.</h2><p>Mathematical models are the cornerstone of our project. We have designed six models that cover various aspects of SugarGain. Not only would these describe the working of our system but they would also show the synergy between our planned experiments and our simulations. The various iterations our models have gone through help us make an informed decision before moving to the lab.</p><div class="row mt-5"><div class="col-sm-5"><h3>Helping us with issues,<br/>And with making new friends too! —<br/>Collaborations.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--img--Home--Modelling.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Model"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Model</span></a></div></div></div></div></section><section class="section6" id="contribution"><div class="container"><div class="row"><div class="content col-lg-9"><h2>Contribution Headline</h2><p>As a team participating in iGEM for the first time, we were absolutely thrilled by the community spirit that iGEM fosters. It was amazing to see how helpful everyone was, and over the years, participating teams had developed into a tightly-knit community, working together on shared goals and aspirations, and having lots of fun along the way!</p><p>To contribute to this amazing community, we developed three softwares: iGEM WikiSync: A continuous deployment tool for iGEM wikis; iGEM WikiSync Github Action: A Github Actions plugin that allows Github repositories to deploy wikis to iGEM servers; and the iGEM Wiki Starter Pack: The easiest way to build iGEM Wikis.</p><div class="row mt-5"><div class="col-sm-5"><h3>Wiki freeze so far<br/>yet awfully close it seems —<br/>WikiSync's here to help.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/6/6a/T--BITSPilani-Goa_India--img--Home--Contribution.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Contribution</span></a></div></div><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/6/6a/T--BITSPilani-Goa_India--img--Home--Contribution.svg"/><h3>Wiki freeze so far<br/>yet awfully close it seems —<br/>WikiSync's here to help.</h3></div></div></div></section><footer><div id="footerNav"><div class="container"><div class="row justify-content-between"><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Project</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Description">Description</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Design">Design</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Model">Model</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Engineering">Engineering</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Safety">Safety</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Implementation">Implementation</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Human Practices</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Human_Practices">Human-Centered Design</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Education">Science Communication</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Parts</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Parts">Parts</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Journal</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Notebook">Notebook</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments">Experiments</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Appendix">Appendix</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Team</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Team">Team</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Attributions">Attributions</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Collaborations">Collaborations</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Contribution</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution">Overview</a></li><li class="py-1"><a 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<html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>SugarGain | iGEM BITS Goa</title><link href="https://2020.igem.org/Template:BITSPilani-Goa_India/css/indexCSS?action=raw&amp;ctype=text/css" rel="stylesheet"/></head><body><nav id="main-nav"><div class="navbar bg-none nav-transparent"><div class="row" id="navbar-row"><a class="navbar-brand" href="https://2020.igem.org/Team:BITSPilani-Goa_India"><i class="navbar-logo"></i><span> Sugar<strong>Gain</strong></span></a><div class="controls h-100 d-flex align-items-center"><div class="align-items-center" id="themeSwitchWrapper"><i class="icon-light"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="icon-dark"></i></div><button class="btn" id="menuSwitch"><div class="menu-icon"></div></button></div></div><div class="row w-100" 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href="https://2020.igem.org/Team:BITSPilani-Goa_India/Collaborations">Collaborations</a></li></ul></div><div class="tab-pane" id="Contribution-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution">Overview</a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Software">Software</a></li></ul></div><div class="tab-pane" id="Entrepreneurship-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Entrepreneurship">Entrepreneurship</a></li></ul></div><div class="tab-pane" id="Judging-pane"><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Judging">Judging</a></li></ul></div></div></div></div><div class="row"><div id="contact-links"><ul><li><a href="https://instagram.com/igem_bits" rel="noopener" target="_blank"><i class="insta"></i><span>Instagram</span></a></li><li><a href="https://twitter.com/igembitsgoa" rel="noopener" target="_blank"><i class="twitter"></i><span>Twitter</span></a></li><li><a href="mailto:igembitsgoa@gmail.com" rel="noopener" target="_blank"><i class="email"></i><span>Email</span></a></li><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Sample"><i class="sample"></i><span>Sample</span></a></li></ul><ul><li><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Acknowledgements" rel="noopener" target="_blank">Acknowledgements</a></li></ul></div></div></div><div class="col-xl-3 col-lg-2"></div></div><div class="row nav-footer w-100"><div class="col-xl-3 col-lg-2"></div><div class="col-xl-6 col-lg-8"></div><div class="col-xl-3 col-lg-2"></div></div></div></div></div></nav><header class="d-flex justify-content-center align-items-center"><video autoplay="true" loop="" muted=""><source src="https://static.igem.org/mediawiki/2020/d/db/T--BITSPilani-Goa_India--video--homepage.mp4" type="video/mp4"/></video></header><section class="section1" id="description"><div class="container"><div class="row"><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/4/43/T--BITSPilani-Goa_India--img--Home--Description.svg"/><h3>A cold winter day —<br/>The bacteria inhibit<br/>Invertase from acting.</h3></div><div class="content col-lg-9"><h2>Tonnes of sugar are lost every year as post-harvest losses. <span>We're here to change that.</span></h2><p></p><p>Sugarcane is one of India’s most important crops. It is cultivated on 5 million hectares of land, making India the second-largest producer of the crop which contributes to 6% of the national GDP and forms the livelihood for 20 million people across the country.</p><p></p><p>This industry is in peril, however. Post-harvest sucrose deterioration is responsible for losses worth millions of rupees. Following its harvest, enzymatic activity reduces sugar recovery rates by upto 10%!</p><p>Our solution mitigates this problem.<br/>We’re SugarGain, and we’re making the world a sweeter place, quite literally!</p><div class="row mt-5"><div class="col-sm-5"><h3>A cold winter day —<br/>The bacteria inhibit<br/>Invertase from acting.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/4/43/T--BITSPilani-Goa_India--img--Home--Description.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Description"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Description</span></a></div></div></div></div></section><section class="section2" id="design"><div class="container"><div class="row"><div class="content col-lg-9"><h2>Design caption goes here.</h2><p>We aim to target invertase present in the sugarcane stem - the enzyme responsible for cleaving sucrose into its constituents. Our project is structured into three major modules: a fructose regulated anti-invertase construct, an atmospherically-regulated kill switch, and a novel polymer inoculant.</p><div class="row mt-5"><div class="col-sm-5"><h3>Reacting to fructose<br/>The bacteria synthesise<br/>Anti-invertase.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/8/87/T--BITSPilani-Goa_India--img--Home--Design.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Design"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Design</span></a></div></div><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/8/87/T--BITSPilani-Goa_India--img--Home--Design.svg"/><h3>Reacting to fructose<br/>The bacteria synthesise<br/>Anti-invertase.</h3></div></div></div></section><section class="section3" id="safety"><div class="container"><div class="row"><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/c/c4/T--BITSPilani-Goa_India--img--Home--Safety.svg"/><h3>The microbe escapes<br/>its confined space. But Alas!<br/>The kill switch goes off.</h3></div><div class="content col-lg-9"><h2><span>Safe, Secure and Responsible.</span></h2><p>Biosafety is a cruicial aspect of our project, especially so when our chassis is so closely involved with food products.</p><p>Throughout our project design we have tried to be cognizant about identifying strategies for robust biosafety. To ensure the highest possible levels of safety, we have developed a three-tier failsafe meachanism after taking into consideration the various use cases of our chassis.</p><div class="row mt-5"><div class="col-sm-5"><h3>The microbe escapes<br/>its confined space. But Alas!<br/>The kill switch goes off.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/c/c4/T--BITSPilani-Goa_India--img--Home--Safety.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Safety"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Safety</span></a></div></div></div></div></section><section class="section4" id="human-practices"><div class="container"><div class="row"><div class="content col-lg-9"><h2><span>Your work affects the world. The world affects your work.</span></h2><p>Human Practices is essential for responsible project design. Our approach to human practices consisted of multiple iterations which allowed us to effectively interview industry experts, researchers and users. Throughout our project, we identified and spoke to various diverse stakeholders relevant to our project.</p><p>We have tried our best to inculcate this feedback at every stage of design of our project.</p><div class="row mt-5"><div class="col-sm-5"><h3>Science and humans,<br/>Like shadow to a body:<br/>Inseparable.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/3/31/T--BITSPilani-Goa_India--img--Home--Human-Practices.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Human_Practices"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Human Practices</span></a></div></div><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/3/31/T--BITSPilani-Goa_India--img--Home--Human-Practices.svg"/><h3>Science and humans,<br/>Like shadow to a body:<br/>Inseparable.</h3></div></div></div></section><section class="section5" id="collaboration"><div class="container"><div class="row"><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--img--Home--Modelling.svg"/><h3>Nature creates math,<br/>We're only trying to<br/>Make math tangible.</h3></div><div class="content col-lg-9"><h2><span>All models are wrong, but some are useful.</span></h2><p>Mathematical models are the cornerstone of our project. We have designed six models that cover various aspects of SugarGain. Not only would these describe the working of our system but they would also show the synergy between our planned experiments and our simulations. The various iterations our models have gone through help us make an informed decision before moving to the lab.</p><div class="row mt-5"><div class="col-sm-5"><h3>Nature creates math,<br/>We're only trying to<br/>Make math tangible.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/4/4c/T--BITSPilani-Goa_India--img--Home--Modelling.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Model"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Model</span></a></div></div></div></div></section><section class="section6" id="contribution"><div class="container"><div class="row"><div class="content col-lg-9"><h2><span>Building your iGEM wiki is now as simple as a git push.</span></h2><p>As a team participating in iGEM for the first time, we were absolutely thrilled by the community spirit that iGEM fosters. It was amazing to see how helpful everyone was, and over the years, participating teams had developed into a tightly-knit community, working together on shared goals and aspirations, and having lots of fun along the way!</p><p>To contribute to this amazing community, we developed three softwares: iGEM WikiSync: A continuous deployment tool for iGEM wikis; iGEM WikiSync Github Action: A Github Actions plugin that allows Github repositories to deploy wikis to iGEM servers; and the iGEM Wiki Starter Pack: The easiest way to build iGEM Wikis.</p><div class="row mt-5"><div class="col-sm-5"><h3>Wiki freeze so far<br/>yet awfully close it seems —<br/>WikiSync's here to help.</h3></div><div class="col-sm-7"><img src="https://static.igem.org/mediawiki/2020/6/6a/T--BITSPilani-Goa_India--img--Home--Contribution.svg"/></div></div><div class="d-flex justify-content-center justify-content-sm-end my-5"><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Contribution</span></a></div></div><div class="sidebar col-lg-3"><img src="https://static.igem.org/mediawiki/2020/6/6a/T--BITSPilani-Goa_India--img--Home--Contribution.svg"/><h3>Wiki freeze so far<br/>yet awfully close it seems —<br/>WikiSync's here to help.</h3></div></div></div></section><footer><div id="footerNav"><div class="container"><div class="row justify-content-between"><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Project</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Description">Description</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Design">Design</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Model">Model</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Engineering">Engineering</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Safety">Safety</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Implementation">Implementation</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Human Practices</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Human_Practices">Human-Centered Design</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Education">Science Communication</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Parts</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Parts">Parts</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Journal</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Notebook">Notebook</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments">Experiments</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Appendix">Appendix</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Team</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Team">Team</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Attributions">Attributions</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Collaborations">Collaborations</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Contribution</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Contribution">Overview</a></li><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Software">Software</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Entrepreneurship</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Entrepreneurship">Entrepreneurship</a></li></ul></div><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Judging</a><ul><li class="py-1"><a href="https://2020.igem.org/Team:BITSPilani-Goa_India/Judging">Judging</a></li></ul></div></div></div></div><div id="footerBrand"><div class="container"><div class="row justify-content-center"><div id="footerTeamLogo"></div><div id="BITSLogo"></div></div><p>iGEM BITS Goa</p><p>Birla Institute of Technology and Science, Pilani</p><p>K K Birla Goa Campus</p><p>NH-17B, Sancoale, Goa, India</p><div class="row justify-content-center" id="footerIcons"><a class="gmail" href="mailto:igembitsgoa@gmail.com"></a><a class="insta" href="https://instagram.com/igem_bits" rel="noopener" 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Revision as of 19:55, 23 October 2020

SugarGain | iGEM BITS Goa

Tonnes of sugar are lost every year as post-harvest losses. We're here to change that.

Sugarcane is one of India’s most important crops. It is cultivated on 5 million hectares of land, making India the second-largest producer of the crop which contributes to 6% of the national GDP and forms the livelihood for 20 million people across the country.

This industry is in peril, however. Post-harvest sucrose deterioration is responsible for losses worth millions of rupees. Following its harvest, enzymatic activity reduces sugar recovery rates by upto 10%!

Our solution mitigates this problem.
We’re SugarGain, and we’re making the world a sweeter place, quite literally!

A cold winter day —
The bacteria inhibit
Invertase from acting.

Design caption goes here.

We aim to target invertase present in the sugarcane stem - the enzyme responsible for cleaving sucrose into its constituents. Our project is structured into three major modules: a fructose regulated anti-invertase construct, an atmospherically-regulated kill switch, and a novel polymer inoculant.

Reacting to fructose
The bacteria synthesise
Anti-invertase.

Safe, Secure and Responsible.

Biosafety is a cruicial aspect of our project, especially so when our chassis is so closely involved with food products.

Throughout our project design we have tried to be cognizant about identifying strategies for robust biosafety. To ensure the highest possible levels of safety, we have developed a three-tier failsafe meachanism after taking into consideration the various use cases of our chassis.

The microbe escapes
its confined space. But Alas!
The kill switch goes off.

Your work affects the world. The world affects your work.

Human Practices is essential for responsible project design. Our approach to human practices consisted of multiple iterations which allowed us to effectively interview industry experts, researchers and users. Throughout our project, we identified and spoke to various diverse stakeholders relevant to our project.

We have tried our best to inculcate this feedback at every stage of design of our project.

Science and humans,
Like shadow to a body:
Inseparable.

All models are wrong, but some are useful.

Mathematical models are the cornerstone of our project. We have designed six models that cover various aspects of SugarGain. Not only would these describe the working of our system but they would also show the synergy between our planned experiments and our simulations. The various iterations our models have gone through help us make an informed decision before moving to the lab.

Nature creates math,
We're only trying to
Make math tangible.

Building your iGEM wiki is now as simple as a git push.

As a team participating in iGEM for the first time, we were absolutely thrilled by the community spirit that iGEM fosters. It was amazing to see how helpful everyone was, and over the years, participating teams had developed into a tightly-knit community, working together on shared goals and aspirations, and having lots of fun along the way!

To contribute to this amazing community, we developed three softwares: iGEM WikiSync: A continuous deployment tool for iGEM wikis; iGEM WikiSync Github Action: A Github Actions plugin that allows Github repositories to deploy wikis to iGEM servers; and the iGEM Wiki Starter Pack: The easiest way to build iGEM Wikis.

Wiki freeze so far
yet awfully close it seems —
WikiSync's here to help.