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| − | + | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>Protocols</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row"><div class="sidebar"><div class="nav" id="contents"><ul></ul></div></div><div class="content fullwidth"><article><div class="accordion" id="collapsibleSections"><div class="card"><div class="card-header" id="headingOne"><h2 class="mb-0"><button aria-controls="collapseOne" aria-expanded="true" class="btn collapsed" data-target="#collapseOne" data-toggle="collapse" type="button"><span>Competent Cell Preparation</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingOne" class="collapse" id="collapseOne"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/5/57/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Competent_Cell_Prep.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/5/57/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Competent_Cell_Prep.pdf" title="Competent Cell Preparation" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTwo"><h2 class="mb-0"><button aria-controls="collapseTwo" aria-expanded="true" class="btn collapsed" data-target="#collapseTwo" data-toggle="collapse" type="button"><span>Invertase Assay Kit</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTwo" class="collapse" id="collapseTwo"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/e/e2/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Invertase_Assay_Kit.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/e/e2/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Invertase_Assay_Kit.pdf" title="Invertase Assay Kit" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingThree"><h2 class="mb-0"><button aria-controls="collapseThree" aria-expanded="true" class="btn collapsed" data-target="#collapseThree" data-toggle="collapse" type="button"><span>Qualitative Analysis of Isolated Plasmid by Gel Electrophoresis</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingThree" class="collapse" id="collapseThree"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/d/df/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Isolated_Plasmid_Analysis.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/d/df/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Isolated_Plasmid_Analysis.pdf" title="Isolated Plasmid Analysis" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFour"><h2 class="mb-0"><button aria-controls="collapseFour" aria-expanded="true" class="btn collapsed" data-target="#collapseFour" data-toggle="collapse" type="button"><span>Plasmid Purification</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFour" class="collapse" id="collapseFour"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/a1/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Plasmid_Purification.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/a1/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Plasmid_Purification.pdf" title="Sorbic Acid and Potassium Sorbate Characterization" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFive"><h2 class="mb-0"><button aria-controls="collapseFive" aria-expanded="true" class="btn collapsed" data-target="#collapseFive" data-toggle="collapse" type="button"><span>LB Medium Preparation</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFive" class="collapse" id="collapseFive"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/4/44/T--BITSPilani-Goa_India--docs--Experiments--Protocols--LB_Medium_Preparation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/4/44/T--BITSPilani-Goa_India--docs--Experiments--Protocols--LB_Medium_Preparation.pdf" title="LB Medium Preparation" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingSix"><h2 class="mb-0"><button aria-controls="collapseSix" aria-expanded="true" class="btn collapsed" data-target="#collapseSix" data-toggle="collapse" type="button"><span>Polymerase Chain Reaction</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingSix" class="collapse" id="collapseSix"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/8/8a/T--BITSPilani-Goa_India--docs--Experiments--Protocols--PCR.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/8/8a/T--BITSPilani-Goa_India--docs--Experiments--Protocols--PCR.pdf" title="Polymerase Chain Reaction" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingSeven"><h2 class="mb-0"><button aria-controls="collapseSeven" aria-expanded="true" class="btn collapsed" data-target="#collapseSeven" data-toggle="collapse" type="button"><span>Site Directed Mutagenesis</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingSeven" class="collapse" id="collapseSeven"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf" title="Site Directed Mutagenesis" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTransformation"><h2 class="mb-0"><button aria-controls="collapseTransformation" aria-expanded="true" class="btn collapsed" data-target="#collapseTransformation" data-toggle="collapse" type="button"><span>Transformation</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTransformation" class="collapse" id="collapseTransformation"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/0/01/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Transformation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/0/01/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Transformation.pdf" title="Transformation" type="application/pdf"/></div></div></div></div></article><div class="d-flex justify-content-between my-5"><a class="button prev" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments/Other"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Other</span></a><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Home</span></a></div></div></div></div></main><footer><div id="footerNav"><div class="container"><div class="row justify-content-between"><div class="col-sm-6 col-md-4 col-lg-3 py-2"><a class="footer-heading">Project</a><ul><li class="py-1"><a 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