Difference between revisions of "Team:BITSPilani-Goa India/Experiments/pFruB-Cra System"
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| − | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>pFruB-Cra System</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row" | + | }</style><header class="d-flex justify-content-center"><div class="custom-container"><div id="header-background"></div><div id="heading"><h1>pFruB-Cra System</h1></div><a href="https://freepik.com" rel="noopener" target="_blank"><span data-placement="top" id="bg-attribution" title="Photo from Freepik"></span></a></div></header><main class="theme-journal"><div class="custom-container"><div class="row"><div class="content fullwidth"><article><div class="accordion" id="collapsibleSections"><div class="card"><div class="card-header" id="headingOne"><h2 class="mb-0"><button aria-controls="collapseOne" aria-expanded="true" class="btn collapsed" data-target="#collapseOne" data-toggle="collapse" type="button"><span>Invertase Inhibitor Assay</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingOne" class="collapse" id="collapseOne"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/0/09/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--Anti-Invertase_Assay.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/0/09/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--Anti-Invertase_Assay.pdf" title="Invertase Inhibitor Assay" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingTwo"><h2 class="mb-0"><button aria-controls="collapseTwo" aria-expanded="true" class="btn collapsed" data-target="#collapseTwo" data-toggle="collapse" type="button"><span>pFruB Characterization</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingTwo" class="collapse" id="collapseTwo"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Characterisation.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Characterisation.pdf" title="pFruB Characterization" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingThree"><h2 class="mb-0"><button aria-controls="collapseThree" aria-expanded="true" class="btn collapsed" data-target="#collapseThree" data-toggle="collapse" type="button"><span>pFruB Specificity</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingThree" class="collapse" id="collapseThree"><div class="card-body"><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/3/3c/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Specificity.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/3/3c/T--BITSPilani-Goa_India--docs--Experiments--pFruB-Cra_System--pFruB_Specificity.pdf" title="pFruB Specificity" type="application/pdf"/></div></div></div><div class="card"><div class="card-header" id="headingFour"><h2 class="mb-0"><button aria-controls="collapseFour" aria-expanded="true" class="btn collapsed" data-target="#collapseFour" data-toggle="collapse" type="button"><span>Site Directed Mutagenesis</span><i class="icon-dropdown"></i></button></h2></div><div aria-labelledby="headingFour" class="collapse" id="collapseFour"><div class="card-body"><p>To tune the Kd value of pFruB, we propose to use the following protocol.</p><p>To download this document, click <a href="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf">here</a>.</p><embed src="https://static.igem.org/mediawiki/2020/a/ae/T--BITSPilani-Goa_India--docs--Experiments--Protocols--Site_Directed_Mutagenesis.pdf" title="Site Directed Mutagenesis" type="application/pdf"/></div></div></div></div></article><div class="d-flex justify-content-between my-5"><a class="button prev" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Experiments</span></a><a class="button next" href="https://2020.igem.org/Team:BITSPilani-Goa_India/Experiments/Kill_Switch"><span class="button-background"><span aria-hidden="" class="circle"><span class="icon arrow"></span></span></span><span class="button-text">Kill Switch</span></a></div></div></div></div></main><footer><div 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