Difference between revisions of "Team:RUM-UPRM/Description"
Paulamanan (Talk | contribs) |
Paulamanan (Talk | contribs) |
||
| Line 73: | Line 73: | ||
<p style="text-indent:40px; margin-left:7%; margin-right:7%"><em>Pseudomonas putida</em> is considered one of the most versatile bacterial strains since it possesses several distinctive characteristics that are required in order to create a viable prototype. This bacterium is Gram-negative and aerobic, requiring oxygen for its metabolism. This saprotrophic bacteria is rod-shaped and measures between 0.5 and 0.8μm. A favorable element that this bacterium provides is the many plasmids it contains, which is a great attribute to apply in synthetic biology mechanisms. It also has the ability to emit green fluorescent pigments.<sup>1</sup> </p> | <p style="text-indent:40px; margin-left:7%; margin-right:7%"><em>Pseudomonas putida</em> is considered one of the most versatile bacterial strains since it possesses several distinctive characteristics that are required in order to create a viable prototype. This bacterium is Gram-negative and aerobic, requiring oxygen for its metabolism. This saprotrophic bacteria is rod-shaped and measures between 0.5 and 0.8μm. A favorable element that this bacterium provides is the many plasmids it contains, which is a great attribute to apply in synthetic biology mechanisms. It also has the ability to emit green fluorescent pigments.<sup>1</sup> </p> | ||
<p style="text-indent:40px; margin-left:7%; margin-right:7%">In order to survive, <em>P. putida</em> requires a temperature between 25-30°C and a neutral pH of 6-8. It is important to highlight that this bacterium can be considered a human pathogen; however, it’s naturally found in soil and is non-pathogenic to plants or animals. Another significant characteristic is the tolerance to environments with metal contamination and the ability to increase resistance to heat and saline stressors through its filamentous phenotype. These characteristics make this organism of great interest for bioremediation and serves as a biosensor for the detection of a variety of pollutants, which we found to be a determining factor in order to apply it to our genetic construct that focuses on the detection and biodegradation of contaminants like mercury and RDX. <sup>1</sup> </p> | <p style="text-indent:40px; margin-left:7%; margin-right:7%">In order to survive, <em>P. putida</em> requires a temperature between 25-30°C and a neutral pH of 6-8. It is important to highlight that this bacterium can be considered a human pathogen; however, it’s naturally found in soil and is non-pathogenic to plants or animals. Another significant characteristic is the tolerance to environments with metal contamination and the ability to increase resistance to heat and saline stressors through its filamentous phenotype. These characteristics make this organism of great interest for bioremediation and serves as a biosensor for the detection of a variety of pollutants, which we found to be a determining factor in order to apply it to our genetic construct that focuses on the detection and biodegradation of contaminants like mercury and RDX. <sup>1</sup> </p> | ||
| − | <img src= "https://static.igem.org/mediawiki/2020/a/ac/T--RUM-UPRM--Pseudomonascellwallstructurewithmergenes.jpeg"> | + | <center> <img src= "https://static.igem.org/mediawiki/2020/a/ac/T--RUM-UPRM--Pseudomonascellwallstructurewithmergenes.jpeg"> </a> </center> |
</div> | </div> | ||
Revision as of 03:19, 27 October 2020
Project Description
Our Problem: Vieques, an Environmental Crisis
Try to scroll this section and look at the navigation list while scrolling!
The Chassis
Pseudomonas putida is considered one of the most versatile bacterial strains since it possesses several distinctive characteristics that are required in order to create a viable prototype. This bacterium is Gram-negative and aerobic, requiring oxygen for its metabolism. This saprotrophic bacteria is rod-shaped and measures between 0.5 and 0.8μm. A favorable element that this bacterium provides is the many plasmids it contains, which is a great attribute to apply in synthetic biology mechanisms. It also has the ability to emit green fluorescent pigments.1
In order to survive, P. putida requires a temperature between 25-30°C and a neutral pH of 6-8. It is important to highlight that this bacterium can be considered a human pathogen; however, it’s naturally found in soil and is non-pathogenic to plants or animals. Another significant characteristic is the tolerance to environments with metal contamination and the ability to increase resistance to heat and saline stressors through its filamentous phenotype. These characteristics make this organism of great interest for bioremediation and serves as a biosensor for the detection of a variety of pollutants, which we found to be a determining factor in order to apply it to our genetic construct that focuses on the detection and biodegradation of contaminants like mercury and RDX. 1
Our Solution: Mer-Nite to the Rescue
Try to scroll this section and look at the navigation list while scrolling!
References
Try to scroll this section and look at the navigation list while scrolling!