Difference between revisions of "Team:RUM-UPRM/Notebook"

 
(31 intermediate revisions by 3 users not shown)
Line 1: Line 1:
 
{{RUM-UPRM}}
 
{{RUM-UPRM}}
 +
{{RUM-UPRM/Nav}}
 +
 
<html lang="en">
 
<html lang="en">
 +
<head>
 +
  <meta charset="utf-8">
 +
  <meta name="viewport" content="width=device-width, initial-scale=1">
 +
  <script src="https://2020.igem.org/wiki/index.php?
title=Template:RUM-UPRM/popper.min&action=raw&ctype=text/javascript"></script>

 +
  
 +
<style>
 +
  body {
 +
    position: relative;
 +
     
  
−
<!--Preloader-->
+
  }
−
<div class="se-pre-con"></div>
+
  ul.nav-pills {
−
<!-- Navigation-->
+
    top: 120px;
−
        <nav class="navbar navbar-expand-lg navbar-light fixed-top" id="mainNav">
+
}
−
            <div class="container">
+
−
                <a class="navbar-brand js-scroll-trigger" href="https://2020.igem.org/Team:RUM-UPRM">RUM-UPRM</a>
+
−
                <button class="navbar-toggler navbar-toggler-right" type="button" data-toggle="collapse" data-target="#navbarResponsive" aria-controls="navbarResponsive" aria-expanded="false" aria-label="Toggle navigation">
+
−
                    Menu
+
−
                    <i class="fas fa-bars"></i>
+
−
                </button>
+
−
                <div class="collapse navbar-collapse" id="navbarResponsive">
+
−
                    <ul class="navbar-nav ml-auto">
+
−
                        <li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://2020.igem.org/Team:RUM-UPRM/Members">Team</a></li>
+
−
                        <li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://2020.igem.org/Team:RUM-UPRM/Description">Projects</a></li>
+
−
<li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://2020.igem.org/Team:RUM-UPRM/Safety">Safety</a></li>
+
−
<li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://2020.igem.org/Team:RUM-UPRM/Engineering">Engineering Success</a></li>
+
−
<li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://2020.igem.org/Team:RUM-UPRM/Human_Practices">Human Practices</a></li>
+
−
<li class="nav-item"><a class="nav-link js-scroll-trigger" href="#">Awards</a></li>
+
−
<li class="nav-item"><a class="nav-link js-scroll-trigger" href="#">Judging Form</a></li>
+
−
                    </ul>
+
−
                </div>
+
−
            </div>
+
−
        </nav>
+
  
−
<body.
+
ul.nav {
−
<section class="bg-light content" style="background-size: 100%;margin-middle: 0em;padding-top: 1.1em;" class="height-90 parallax">
+
position: sticky;
−
<div class="d-flex h-100 align-items-center">
+
 
−
           <h3 class="display-5">Lab Protocols</h3>
+
  }
 +
  div.col-8 div {
 +
    height: 500px;
 +
  }
 +
.sticky {
 +
    position: relative;
 +
    top: 0;
 +
    width: 100%;
 +
}
 +
#myScrollspy {
 +
position: sticky;
 +
top: 70px;
 +
 
 +
}
 +
 
 +
  </style>
 +
</head>
 +
<body data-spy="scroll" data-target="#myScrollspy" data-offset="1">
 +
 
 +
<div class="container-fluid" style= "background-color:#3BB9FF; height:100px; top: 200px">
 +
  <center><h1>Notebook</h1></center>
 +
</div>
 +
 
 +
 
 +
<div class="container-fluidtwo">
 +
  <div class="row">
 +
    <nav class="col-sm-2 col-3" id="myScrollspy">
 +
      <ul class="nav nav-pills flex-sm-column">
 +
<li class="nav-item">
 +
           <a class="nav-link active" href="#section1">Overview</a>
 +
        </li>
 +
        <li class="nav-item">
 +
          <a class="nav-link active" href="#section2">Ligation and Digestion</a>
 +
        </li>
 +
        <li class="nav-item">
 +
          <a class="nav-link" href="#section3">DNA Extraction</a>
 +
        </li>
 +
        <li class="nav-item">
 +
          <a class="nav-link" href="#section4">DNA Precipitation</a>
 +
        </li>
 +
<li class="nav-item">
 +
          <a class="nav-link" href="#section5">DNA Gel Extraction</a>
 +
        </li>
 +
<li class="nav-item">
 +
          <a class="nav-link" href="#section6">Bacterial Transformation</a>
 +
        </li>
 +
<li class="nav-item">
 +
          <a class="nav-link" href="#section7">Preparation of Competent Cells</a>
 +
        </li>
 +
<li class="nav-item">
 +
          <a class="nav-link" href="#section8">Gibson Assembly</a>
 +
        </li>
 +
 
 +
      </ul>
 +
    </nav>
 +
 
 +
<div class="col-sm-9 col-8; scroll"> <div align="justify">
 +
      <div id="section1" style="background-color:rgba(0,0,0,0)">   
 +
        <center><h1>Overview</h1></center>
 +
 
 +
        <p style="margin-left:7%; margin-right:7%; text-indent:5%"> Laboratory Technique Workshops were given to all team members in order to be prepared to perform in the wet lab. The following protocols were discussed and practiced. Unfortunately, plans changed with COVID 19. We are looking forward to the moment we can go back to our lab and apply all this knowledge.</p>
 +
</div>
 +
<center><img src="https://static.igem.org/mediawiki/parts/1/15/T--RUM-UPRM--labwork.png" style="width: 50%"> </center>
 +
   
 +
      <div id="section2" style="background-color:rgba(0,0,0,0)">  
  
 
<div id="accordion">
 
<div id="accordion">
−
  <div class="card">
+
 
 +
<style>
 +
.accordion {
 +
  background-color:rgb(60,179,113);
 +
  color: rgba(255,255,255,0.9);
 +
  cursor: pointer;
 +
  padding: 24px;
 +
  width: 100%;
 +
  text-align: center;
 +
  outline: none;
 +
  font-size: 18px;
 +
  border: 1px solid black;
 +
  border-radius: 16px
 +
}
 +
  </style>
 +
 
 +
<div class="text-center">
 +
<div class="card">
 
     <div class="card-header" id="headingOne">
 
     <div class="card-header" id="headingOne">
 
     <h5 class="mb-0">
 
     <h5 class="mb-0">
Line 44: Line 117:
 
       <div class="card-body">
 
       <div class="card-body">
 
   <div class="content">
 
   <div class="content">
−
   <object data="https://static.igem.org/mediawiki/2020/1/11/T--RUM-UPRM--LigationProtocol.pdf" width="100%" height="600" />
+
   <object data="https://static.igem.org/mediawiki/2020/1/11/T--RUM-UPRM--LigationProtocol.pdf" width="50%" height="500" />
 
     </object>
 
     </object>
 
       </div>
 
       </div>
 
       </div>
 
       </div>
 
       </div>
 
       </div>
−
  <div class="card">
+
</div>
 +
 
 +
      <div id="section3">
 +
<div class="card">
 
     <div class="card-header" id="headingTwo">
 
     <div class="card-header" id="headingTwo">
 
     <h5 class="mb-0">
 
     <h5 class="mb-0">
Line 60: Line 136:
 
       <div class="card-body">
 
       <div class="card-body">
 
   <div class="content">
 
   <div class="content">
−
   <object data="https://static.igem.org/mediawiki/2020/e/e1/T--RUM-UPRM--DNAEXTRACTION.pdf" type="application/pdf" width="100%" height="600" />
+
   <object data="https://static.igem.org/mediawiki/2020/e/e1/T--RUM-UPRM--DNAEXTRACTION.pdf" type="application/pdf" width="50%" height="500" />
 
     </object>
 
     </object>
 
       </div>
 
       </div>
 
       </div>
 
       </div>
 
       </div>
 
       </div>
−
  <div class="card">
+
</div>
−
     <div class="card-header" id="headingFour">
+
       
 +
      <div id="section4" style="background-color:rgba(0,0,0,0)">       
 +
<div class="card">
 +
     <div class="card-header" id="headingThree">
 
       <h5 class="mb-0">
 
       <h5 class="mb-0">
−
         <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
+
         <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseThree" aria-expanded="false" aria-controls="collapseThree">
 
           DNA Precipitation
 
           DNA Precipitation
 
         </button>
 
         </button>
Line 75: Line 154:
 
     <div id="collapseThree" class="collapse" aria-labelledby="headingThree" data-parent="#accordion">
 
     <div id="collapseThree" class="collapse" aria-labelledby="headingThree" data-parent="#accordion">
 
     <div class="content">
 
     <div class="content">
−
   <object data="https://static.igem.org/mediawiki/2020/2/23/T--RUM-UPRM--DNAPRECIPITATION.pdf" type="application/pdf" width="100%" height="600" />
+
   <object data="https://static.igem.org/mediawiki/2020/2/23/T--RUM-UPRM--DNAPRECIPITATION.pdf" type="application/pdf" width="50%" height="500" />
 
       </object>
 
       </object>
 
       </div>
 
       </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
 +
</div>
 +
 +
 +
      <div id="section5" style="background-color:rgba(0,0,0,0)">       
 
<div class="card">
 
<div class="card">
 
     <div class="card-header" id="headingFour">
 
     <div class="card-header" id="headingFour">
−
      <h5 class="mb-0">
+
    <h5 class="mb-0">
 
         <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
 
         <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseFour" aria-expanded="false" aria-controls="collapseFour">
−
          DNA Gel Extraction
+
        DNA Gel Extraction
 
         </button>
 
         </button>
 
       </h5>
 
       </h5>
 
     </div>
 
     </div>
−
     <div id="collapseFour" class="collapse show" aria-labelledby="headingFour" data-parent="#accordion">
+
     <div id="collapseFour" class="collapse" aria-labelledby="headingFour" data-parent="#accordion">
−
    <div class="content">
+
      <div class="card-body">
−
<object data="https://static.igem.org/mediawiki/2020/0/07/T--RUM-UPRM--Gel_extraction.pdf" width="100%" height="600" />
+
  <div class="content">
−
</object>    
+
  <object data="https://static.igem.org/mediawiki/2020/0/07/T--RUM-UPRM--Gel_extraction.pdf" width="50%" height="500" />
−
</div>
+
    </object>
 +
      </div>
 +
      </div>
 +
      </div>
 +
</div>
 +
 
 +
      <div id="section6" style="background-color:rgba(0,0,0,0)">       
 +
 
 +
<div class="card">
 +
    <div class="card-header" id="headingFive">
 +
    <h5 class="mb-0">
 +
        <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseFive" aria-expanded="false" aria-controls="collapseFive">
 +
        Bacterial Transformation
 +
        </button>
 +
      </h5>
 
     </div>
 
     </div>
 +
    <div id="collapseFive" class="collapse" aria-labelledby="headingFive" data-parent="#accordion">
 +
      <div class="card-body">
 +
  <div class="content">
 +
  <object data="https://static.igem.org/mediawiki/2020/a/ac/T--RUM-UPRM--Bacterial_Transformation.pdf" width="50%" height="500" />
 +
    </object>
 +
      </div>
 
       </div>
 
       </div>
 +
      </div>
 +
</div>
  
 +
<div id="section7" style="background-color:rgba(0,0,0,0)">       
 +
<div class="card">
 +
    <div class="card-header" id="headingSix">
 +
    <h5 class="mb-0">
 +
        <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseSix" aria-expanded="false" aria-controls="collapseSix">
 +
        Preparation of Competent Cells
 +
        </button>
 +
      </h5>
 +
    </div>
 +
    <div id="collapseSix" class="collapse" aria-labelledby="headingSix" data-parent="#accordion">
 +
      <div class="card-body">
 +
  <div class="content">
 +
  <object data="https://static.igem.org/mediawiki/2020/d/dc/T--RUM-UPRM--Competent_Cells.pdf" width="50%" height="500" />
 +
    </object>
 +
      </div>
 +
      </div>
 +
      </div>
 +
</div>
 +
 +
 +
<div id="section8" style="background-color:rgba(0,0,0,0)">       
 +
<div class="card">
 +
    <div class="card-header" id="headingSeven">
 +
    <h5 class="mb-0">
 +
        <button class="btn btn-link collapsed" data-toggle="collapse" data-target="#collapseSeven" aria-expanded="false" aria-controls="collapseSeven">
 +
        Gibson Assembly
 +
        </button>
 +
      </h5>
 +
    </div>
 +
    <div id="collapseSeven" class="collapse" aria-labelledby="headingSeven" data-parent="#accordion">
 +
      <div class="card-body">
 +
  <div class="content">
 +
  <object data="https://static.igem.org/mediawiki/2020/3/30/T--RUM-UPRM--GibsonAssembly.pdf" width="50%" height="500" />
 +
    </object>
 +
      </div>
 +
      </div>
 +
      </div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
</div>
 +
    </div>
 +
  </div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
</div>
 +
 +
<script>
 +
window.onscroll = function() {myFunction()};
 +
 +
var myScrollspy = document.getElementById("myScrollspy");
 +
var sticky = myScrollspy.offsetTop+70px;
 +
 +
function myFunction() {
 +
  if (window.pageYOffset >= sticky) {
 +
    myScrollspy.classList.add("sticky")
 +
  } else {
 +
    myScrollspy.classList.remove("sticky");
 +
  }
 +
}
 +
</script>
 
    
 
    
 
</body>
 
</body>
−
      </div>
 
−
</section>
 
−
<footer class="footer bg-black small text-center text-white-50"><div class="container">Copyright © UPRM-RUM 2020</div></footer>
 
−
    </body>
 
 
</html>
 
</html>
 +
 +
{{RUM-UPRM/Footer}}

Latest revision as of 02:53, 28 October 2020


RUM-UPRM Wiki Source Code




Notebook

Overview

Laboratory Technique Workshops were given to all team members in order to be prepared to perform in the wet lab. The following protocols were discussed and practiced. Unfortunately, plans changed with COVID 19. We are looking forward to the moment we can go back to our lab and apply all this knowledge.


Our Sponsors

Amgen Snapgene UPRM Goya Genscript PRABIA MolecularCloud MatLab IDT ReviveRestore Twist

Follows Us!