Difference between revisions of "Team:Virginia"

 
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      <div class="menulogo">MANIFOLD</div>
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            <a class="mainitem" href="https://2020.igem.org/Team:Virginia">HOME</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia#abstract">Abstract</a>
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            <a class="mainitem" href="#project">PROJECT</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Model">Modeling</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Engineering">Engineering</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Implementation">Implementation</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a>
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            <a class="mainitem" href="#parts">PARTS</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> Parts</a>
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            <a class="mainitem" href="#outreach">OUTREACH</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Human_Practices">Human Practices</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Public_engagement">Public Engagement</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Collaborations">Collaborations</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Contribution">Contributions</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Entrepreneurship">Entrepreneurship</a>
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            <a class="mainitem" href="#team">TEAM</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a>
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            <a class="mainitem" href="#awards">AWARDS</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Awards">Awards</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Medals">Medals</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers">Sources</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a>
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a>
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<h1> Welcome to iGEM 2020! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li> <a href="https://2020.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2020.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2020.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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              Through the use of Bacterial Microcompartments and DNA Scaffolds,
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              We're revolutionizing biosynthesis with intracellular nanoreactors.
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            <a class="left13" href="https://2020.igem.org/Team:Virginia/Description">
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<h3> Styling your wiki </h3>
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              Find out more in our project description &gt;
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2020 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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            <svg overflow="hidden" preserveAspectRatio="xMidYMid meet" viewBox="0 0 1280 720" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><defs><clipPath id="clip0"><rect height="720" width="1280" x="0" y="0"></rect></clipPath></defs><g clip-path="url(#clip0)"><rect fill="#FFFFFF" fill-opacity="0" height="720" width="1280" x="0" y="0"></rect><path d="M576.018 589.423 547.94 645.5 171.203 645.5 28.5001 360.5 171.203 75.5001 547.94 75.5001 576.5 132.54" fill="none" fill-rule="evenodd" stroke="#F7F7F7" stroke-miterlimit="8" stroke-width="33.3333"></path><text fill="#F7F7F7" font-family="'THICCCBOI',THICCCBOI_MSFontService,sans-serif" font-size="253" font-weight="700" transform="translate(110.667 430)">Abstract</text></g></svg>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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            The lack of a versatile and reliable way to improve <div class="dict">metabolic flux <span>Metabolic flux is the rate of turnover of molecules through a metabolic pathway. Flux is regulated by the enzymes involved in a pathway. Within cells, regulation of flux is vital for all metabolic pathways to regulate the pathway's activity under different conditions.</span></div>channeling, pathway <div class="dict"> orthogonality<span>The growth-independant optimization of a pathway,for the production of a target chemical. These pathways are characterized by the minimization of interactions between the chemical-producing pathways and the biomass-producing pathways. Physiologically, this means in perfect orthogonal networks,the product pathway shares no enzymatic steps with cellular pathways that are responsible for the production of precursors required for biomass and, only a single metabolite serves as a branch point from which product and biomass pathways diverge.</span></div>, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of <div class="dict">bacterial microcompartments<span>Bacterial microcompartments (BMCs) are organelle-like structures, consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable.</span></div> (BMCs) with encapsulated <div class="dict">dsDNA scaffold <span>Non-coding linear DNA containing repetitive sequence specific regions for zinc-finger protein-domain ligation. This work is used in DNA origami to assemble precise nanoscale structures with custom geometries and fully addressable surfaces.</span></div> that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as <div class="dict">zinc-finger fusion proteins<span>A zinc finger is a small, functional, independently folded domain that coordinates one or more zinc ions to stabilize its structure through cysteine and/or histidine residues. Zinc fingers are structurally diverse and exhibit a wide range of functions, from DNA- or RNA-binding to protein-protein interactions and membrane association</span></div>. Here we deliver the designs for an <i>E. coli</i> cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis.  
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UPLOAD FILES
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          <img class="hvr-wobble-horizontal" onclick="window.location.href='https://2020.igem.org/Team:Virginia/Description'" src="https://static.igem.org/mediawiki/2020/d/d6/T--Virginia--images--bact4.SVG"/>
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<h3> Wiki template information </h3>
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          <img class="hvr-wobble-to-top-right" onclick="window.location.href='https://2020.igem.org/Team:Virginia/Experiments'" src="https://static.igem.org/mediawiki/2020/d/d3/T--Virginia--images--bact3.SVG"/>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2020.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>  
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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          <source src="https://static.igem.org/mediawiki/2020/c/ce/T--Virginia--media--promVid.mp4" type="video/mp4"/>
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EDIT PAGE
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<h3>Tips</h3>
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          <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/9/96/T--Virginia--sponsors--Benchling.png"/></div>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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          <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/6/69/T--Virginia--sponsors--qiagen.png"/></div>
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<ul>
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          <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/f/f5/T--Virginia--sponsors--lucid.png"/></div>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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          <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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          <div class="menulogo">MANIFOLD</div>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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          <div class="followus">FOLLOW US:</div>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2020.igem.org/Calendar">iGEM 2020 calendar</a> </li>
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          <div class="sociallink"><div style="color:#7496D2;margin-right: 1em;">EMAIL:</div> VAiGEM2020@GMAIL.COM</div>
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<li>Have lots of fun! </li>
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          <div class="sociallink"><div style="color:#FF99FF;margin-right: 1em;">INSTAGRAM: </div>@iGEM.AT.UVA</div>
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          <div class="sociallink"><div style="color:#A3E7FF;margin-right: 1em;">TWITTER: </div>@VIRGINIA_iGEM</div>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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      <img src="https://static.igem.org/mediawiki/2020/1/17/T--Virginia--logo-up-frames--2.svg"/>
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      <img src="https://static.igem.org/mediawiki/2020/9/95/T--Virginia--logo-up-frames--3.svg"/>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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      <img src="https://static.igem.org/mediawiki/2020/3/35/T--Virginia--logo-up-frames--4.svg"/>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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      <img src="https://static.igem.org/mediawiki/2020/b/bc/T--Virginia--logo-up-frames--5.svg"/>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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      <img src="https://static.igem.org/mediawiki/2020/9/9b/T--Virginia--logo-up-frames--6.svg"/>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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      <img src="https://static.igem.org/mediawiki/2020/6/65/T--Virginia--logo-up-frames--7.svg"/>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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      <img src="https://static.igem.org/mediawiki/2020/b/b4/T--Virginia--logo-up-frames--9.svg"/>
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Latest revision as of 03:31, 28 October 2020

Manifold

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Through the use of Bacterial Microcompartments and DNA Scaffolds,
We're revolutionizing biosynthesis with intracellular nanoreactors.
Find out more in our project description >
Abstract
The lack of a versatile and reliable way to improve
metabolic flux Metabolic flux is the rate of turnover of molecules through a metabolic pathway. Flux is regulated by the enzymes involved in a pathway. Within cells, regulation of flux is vital for all metabolic pathways to regulate the pathway's activity under different conditions.
channeling, pathway
orthogonalityThe growth-independant optimization of a pathway,for the production of a target chemical. These pathways are characterized by the minimization of interactions between the chemical-producing pathways and the biomass-producing pathways. Physiologically, this means in perfect orthogonal networks,the product pathway shares no enzymatic steps with cellular pathways that are responsible for the production of precursors required for biomass and, only a single metabolite serves as a branch point from which product and biomass pathways diverge.
, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of
bacterial microcompartmentsBacterial microcompartments (BMCs) are organelle-like structures, consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable.
(BMCs) with encapsulated
dsDNA scaffold Non-coding linear DNA containing repetitive sequence specific regions for zinc-finger protein-domain ligation. This work is used in DNA origami to assemble precise nanoscale structures with custom geometries and fully addressable surfaces.
that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as
zinc-finger fusion proteinsA zinc finger is a small, functional, independently folded domain that coordinates one or more zinc ions to stabilize its structure through cysteine and/or histidine residues. Zinc fingers are structurally diverse and exhibit a wide range of functions, from DNA- or RNA-binding to protein-protein interactions and membrane association
. Here we deliver the designs for an E. coli cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis.
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