Difference between revisions of "Team:Virginia"
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| − | <a class=" | + | <a class="mainitem" href="https://2020.igem.org/Team:Virginia">HOME</a> |
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a> | ||
| − | <a class="hvr-sweep-to-right" href="#abstract">Abstract | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia#abstract">Abstract</a> |
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<a class="mainitem" href="#project">PROJECT</a> | <a class="mainitem" href="#project">PROJECT</a> | ||
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a> | ||
| − | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/ | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Model">Modeling</a> |
| − | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/ | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Engineering">Engineering</a> |
| + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Implementation">Implementation</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a> | ||
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<a class="mainitem" href="#parts">PARTS</a> | <a class="mainitem" href="#parts">PARTS</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| − | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> Parts</a> |
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<a class="mainitem" href="#outreach">OUTREACH</a> | <a class="mainitem" href="#outreach">OUTREACH</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| − | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/ | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Human_Practices">Human Practices</a> |
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Public_engagement">Public Engagement</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Public_engagement">Public Engagement</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Collaborations">Collaborations</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Collaborations">Collaborations</a> | ||
| − | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/ | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Contribution">Contributions</a> |
| + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Entrepreneurship">Entrepreneurship</a> | ||
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<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a> | ||
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<a class="mainitem" href="#about">RESOURCES</a> | <a class="mainitem" href="#about">RESOURCES</a> | ||
<div class="dropdown-content"> | <div class="dropdown-content"> | ||
| − | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers"> | + | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers">Sources</a> |
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a> | ||
<a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a> | <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a> | ||
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| − | + | Through the use of Bacterial Microcompartments and DNA Scaffolds, | |
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| − | + | We're revolutionizing biosynthesis with intracellular nanoreactors. | |
| − | + | </div> | |
| − | + | <a class="left13" href="https://2020.igem.org/Team:Virginia/Description"> | |
| − | + | Find out more in our project description > | |
| − | + | </a> | |
| − | </ | + | </div> |
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| − | </ | + | <img class="pathBG" src="https://static.igem.org/mediawiki/2020/d/d6/T--Virginia--images--pathwaybackground.png"/> |
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| + | <img class="indexBMC" src="https://static.igem.org/mediawiki/2020/d/d7/T--Virginia--images--indexBMC.svg"/> | ||
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| + | <div id="abstract"></div> | ||
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| + | <img class="labImg" src="https://static.igem.org/mediawiki/2020/9/94/T--Virginia--images--lab.png"/> | ||
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| + | <svg overflow="hidden" preserveAspectRatio="xMidYMid meet" viewBox="0 0 1280 720" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><defs><clipPath id="clip0"><rect height="720" width="1280" x="0" y="0"></rect></clipPath></defs><g clip-path="url(#clip0)"><rect fill="#FFFFFF" fill-opacity="0" height="720" width="1280" x="0" y="0"></rect><path d="M576.018 589.423 547.94 645.5 171.203 645.5 28.5001 360.5 171.203 75.5001 547.94 75.5001 576.5 132.54" fill="none" fill-rule="evenodd" stroke="#F7F7F7" stroke-miterlimit="8" stroke-width="33.3333"></path><text fill="#F7F7F7" font-family="'THICCCBOI',THICCCBOI_MSFontService,sans-serif" font-size="253" font-weight="700" transform="translate(110.667 430)">Abstract</text></g></svg> | ||
| + | </div> | ||
| + | </div> | ||
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| + | <div class="abstractHolder"> | ||
| + | The lack of a versatile and reliable way to improve <div class="dict">metabolic flux <span>Metabolic flux is the rate of turnover of molecules through a metabolic pathway. Flux is regulated by the enzymes involved in a pathway. Within cells, regulation of flux is vital for all metabolic pathways to regulate the pathway's activity under different conditions.</span></div>channeling, pathway <div class="dict"> orthogonality<span>The growth-independant optimization of a pathway,for the production of a target chemical. These pathways are characterized by the minimization of interactions between the chemical-producing pathways and the biomass-producing pathways. Physiologically, this means in perfect orthogonal networks,the product pathway shares no enzymatic steps with cellular pathways that are responsible for the production of precursors required for biomass and, only a single metabolite serves as a branch point from which product and biomass pathways diverge.</span></div>, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of <div class="dict">bacterial microcompartments<span>Bacterial microcompartments (BMCs) are organelle-like structures, consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable.</span></div> (BMCs) with encapsulated <div class="dict">dsDNA scaffold <span>Non-coding linear DNA containing repetitive sequence specific regions for zinc-finger protein-domain ligation. This work is used in DNA origami to assemble precise nanoscale structures with custom geometries and fully addressable surfaces.</span></div> that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as <div class="dict">zinc-finger fusion proteins<span>A zinc finger is a small, functional, independently folded domain that coordinates one or more zinc ions to stabilize its structure through cysteine and/or histidine residues. Zinc fingers are structurally diverse and exhibit a wide range of functions, from DNA- or RNA-binding to protein-protein interactions and membrane association</span></div>. Here we deliver the designs for an <i>E. coli</i> cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis. | ||
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| + | <div class="miniBlock"> | ||
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| + | <img class="hvr-wobble-horizontal" onclick="window.location.href='https://2020.igem.org/Team:Virginia/Description'" src="https://static.igem.org/mediawiki/2020/d/d6/T--Virginia--images--bact4.SVG"/> | ||
| + | </div> | ||
| + | <div> | ||
| + | <img class="hvr-wobble-to-top-right" onclick="window.location.href='https://2020.igem.org/Team:Virginia/Experiments'" src="https://static.igem.org/mediawiki/2020/d/d3/T--Virginia--images--bact3.SVG"/> | ||
| + | </div> | ||
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| + | <img class="hvr-wobble-vertical" onclick="window.location.href='https://2020.igem.org/Team:Virginia/Model'" src="https://static.igem.org/mediawiki/2020/0/03/T--Virginia--images--bact1.SVG"/> | ||
| + | </div> | ||
| + | <div> | ||
| + | <img class="hvr-wobble-to-bottom-right" onclick="window.location.href='https://2020.igem.org/Team:Virginia/Human_Practices'" src="https://static.igem.org/mediawiki/2020/3/38/T--Virginia--images--bact2.SVG"/> | ||
| + | </div> | ||
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| + | <video class="promVid" controls="" playsinline="" poster="https://static.igem.org/mediawiki/2020/b/be/T--Virginia--images--promVidThumb.png"> | ||
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<div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/d4/T--Virginia--sponsors--UVA.png"/></div> | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/d4/T--Virginia--sponsors--UVA.png"/></div> | ||
<div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div> | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div> | ||
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/4/41/T--Virginia--images--neb.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/e/e6/T--Virginia--images--twist.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/4/41/T--Virginia--images--cvillbiohub.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/b/b8/T--Virginia--sponsors--addgene.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/7/75/T--Virginia--sponsors--matlab.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/9/96/T--Virginia--sponsors--Benchling.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/6/69/T--Virginia--sponsors--qiagen.png"/></div> |
| − | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/ | + | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/f/f5/T--Virginia--sponsors--lucid.png"/></div> |
<div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div> | <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div> | ||
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<div class="menulogo">MANIFOLD</div> | <div class="menulogo">MANIFOLD</div> | ||
<div class="followus">FOLLOW US:</div> | <div class="followus">FOLLOW US:</div> | ||
| − | <div class="sociallink"><div style="color:#7496D2;margin-right: 1em;"> | + | <div class="sociallink"><div style="color:#7496D2;margin-right: 1em;">EMAIL:</div> VAiGEM2020@GMAIL.COM</div> |
<div class="sociallink"><div style="color:#FF99FF;margin-right: 1em;">INSTAGRAM: </div>@iGEM.AT.UVA</div> | <div class="sociallink"><div style="color:#FF99FF;margin-right: 1em;">INSTAGRAM: </div>@iGEM.AT.UVA</div> | ||
| − | <div class="sociallink"><div style="color:#A3E7FF;margin-right: 1em;">TWITTER: </div>@ | + | <div class="sociallink"><div style="color:#A3E7FF;margin-right: 1em;">TWITTER: </div>@VIRGINIA_iGEM</div> |
</div> | </div> | ||
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Latest revision as of 03:31, 28 October 2020
see more
Through the use of Bacterial Microcompartments and DNA Scaffolds,
We're revolutionizing biosynthesis with intracellular nanoreactors.
Find out more in our project description >
The lack of a versatile and reliable way to improve
metabolic flux Metabolic flux is the rate of turnover of molecules through a metabolic pathway. Flux is regulated by the enzymes involved in a pathway. Within cells, regulation of flux is vital for all metabolic pathways to regulate the pathway's activity under different conditions.
channeling, pathway orthogonalityThe growth-independant optimization of a pathway,for the production of a target chemical. These pathways are characterized by the minimization of interactions between the chemical-producing pathways and the biomass-producing pathways. Physiologically, this means in perfect orthogonal networks,the product pathway shares no enzymatic steps with cellular pathways that are responsible for the production of precursors required for biomass and, only a single metabolite serves as a branch point from which product and biomass pathways diverge.
, and product yields is a major impediment to the expanded utilization of biosynthesis for the production of drugs and industrially valuable chemicals. Manifold, a platform technology that addresses this problem, consists of bacterial microcompartmentsBacterial microcompartments (BMCs) are organelle-like structures, consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40-200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable.
(BMCs) with encapsulated dsDNA scaffold Non-coding linear DNA containing repetitive sequence specific regions for zinc-finger protein-domain ligation. This work is used in DNA origami to assemble precise nanoscale structures with custom geometries and fully addressable surfaces.
that sequester and spatially organize, at fixed concentrations, biosynthetic enzymes presented as zinc-finger fusion proteinsA zinc finger is a small, functional, independently folded domain that coordinates one or more zinc ions to stabilize its structure through cysteine and/or histidine residues. Zinc fingers are structurally diverse and exhibit a wide range of functions, from DNA- or RNA-binding to protein-protein interactions and membrane association
. Here we deliver the designs for an E. coli cell capable of synthesizing resveratrol using the Manifold platform. The Manifold platform will help lower costs and expand the applications of chemical biosynthesis.
