Difference between revisions of "Team:Virginia/Protocols"

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       Top filler<br/> write out or link our protocols here!! <br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>
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      <div class="dict">temporary<span><img src="https://upload.wikimedia.org/wikipedia/commons/thumb/2/25/Carboxysome_and_bacterial_microcompartments.jpg/800px-Carboxysome_and_bacterial_microcompartments.jpg"/>Bacterial microcompartments (BMCs) are organelle-like structures, consisting of a protein shell that encloses enzymes and other proteins. BMCs are typically about 40–200 nanometers in diameter and are entirely made of proteins. The shell functions like a membrane, as it is selectively permeable.</span></div> filler
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            Index:
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      <br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/>temporary filler<br/> temporary <div class="dict">filler<span>DNA origami is the nanoscale folding of DNA to create non-arbitrary two- and three-dimensional shapes at the nanoscale. The specificity of the interactions between complementary base pairs make DNA a useful construction material, through design of its base sequences. DNA is a well-understood material that is suitable for creating scaffolds that hold other molecules in place or to create structures all on its own.</span></div><br/>  
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          <div>Lab Notebook</div>
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        <div class="sectionTitle" id="Intro">Introduction</div>
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            Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the <a href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> page. For information on the planned use of these protocols, see the <a href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> page.
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        <div class="sectionTitle" id="September">Protocols</div>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/d/d6/T--Virginia--Protocols--0.8_Percent_Agarose_Gel_Creation_and_Pour.pdf" target="_blank">Agarose Gel Creation and Pour</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/8/80/T--Virginia--Protocols--Agarose_Gel_Electrophoresis.pdf" target="_blank">Agarose Gel Electrophoresis</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/6/6b/T--Virginia--Protocols--Culture_from_Agar_Stab_%28Inoculation%29.pdf" target="_blank">Culture from Agar Stab (Inoculation)</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/c/c6/T--Virginia--Protocols--DpnI_Incubation.pdf" target="_blank">DpnI Incubation</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/5/5c/T--Virginia--Protocols--Glycerol_Stock.pdf" target="_blank">Glycerol Stock</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/0/02/T--Virginia--Protocols--Golden_Gate_Assembly_with_BsaI.pdf" target="_blank">Golden Gate Assembly with BsaI</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/9/94/T--Virginia--Protocols--Golden_Gate_Assembly_with_BsmBI.pdf" target="_blank">Golden Gate Assembly with BsmBI</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/1/19/T--Virginia--Protocols--NEB_Time-Saver_qualified_Restriction_Enzyme_Digestion.pdf" target="_blank">NEB Time-Saver qualified Restriction Enzyme Digestion</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/3/3a/T--Virginia--Protocols--NEBuilder_Hifi_Mutagenesis.pdf" target="_blank">NEBuilder Hifi Mutagenesis</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/9/9d/T--Virginia--Protocols--PCR_Purification_Kit_%28GenScript%29.pdf" target="_blank">PCR Purification Kit (GenScript)</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/4/45/T--Virginia--Protocols--Phusion_PCR_Protocol.pdf" target="_blank">Phusion PCR Protocol</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/6/61/T--Virginia--Protocols--QIAGEN_HiSpeed_Plasmid_Purification.pdf" target="_blank">QIAGEN HiSpeed Plasmid Purification</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/3/31/T--Virginia--Protocols--QIAprep_Spin_Miniprep_Kit.pdf" target="_blank">QIAprep Spin Miniprep Kit</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/5/5a/T--Virginia--Protocols--Restriction_Enzyme%28s%29_Digest.pdf" target="_blank">Restriction Enzyme(s) Digest</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/3/3b/T--Virginia--Protocols--Site_Directed_Mutagenesis.pdf" target="_blank">Site Directed Mutagenesis</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/7/7f/T--Virginia--Protocols--Standard_Assembly_%28Column_3_-_pduD_%2B_GFP%29.pdf" target="_blank">Standard Assembly (Column 3 - pduD + GFP)</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/2/25/T--Virginia--Protocols--Standard_Assembly_%28Combining_Column_1_and_3%29.pdf" target="_blank">Standard Assembly (Combining Column 1 and 3)</a><br/>
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            <img class="icon" src="https://static.igem.org/mediawiki/2020/d/dd/T--Virginia--images--PageIcon.svg"/><a href="https://static.igem.org/mediawiki/2020/0/04/T--Virginia--Protocols--Transforming_Competent_Cells.pdf" target="_blank">Transforming Competent Cells</a><br/>
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Latest revision as of 03:32, 28 October 2020

Manifold

HOME
Main Abstract
PROJECT
Description Design Experiments Results Modeling Engineering Implementation Notebook Safety
PARTS
Parts
OUTREACH
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TEAM
Members Attributions
AWARDS
Awards Medals
RESOURCES
Sources Nucleic Acids Protocols
Index:
Lab Notebook
Introduction
Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the Notebook page. For information on the planned use of these protocols, see the Experiments page.
Protocols
Agarose Gel Creation and Pour
Agarose Gel Electrophoresis
Culture from Agar Stab (Inoculation)
DpnI Incubation
Glycerol Stock
Golden Gate Assembly with BsaI
Golden Gate Assembly with BsmBI
NEB Time-Saver qualified Restriction Enzyme Digestion
NEBuilder Hifi Mutagenesis
PCR Purification Kit (GenScript)
Phusion PCR Protocol
QIAGEN HiSpeed Plasmid Purification
QIAprep Spin Miniprep Kit
Restriction Enzyme(s) Digest
Site Directed Mutagenesis
Standard Assembly (Column 3 - pduD + GFP)
Standard Assembly (Combining Column 1 and 3)
Transforming Competent Cells
TRIzol DNA Extraction
FOLLOW US: