Difference between revisions of "Team:Virginia/Protocols"

 
(10 intermediate revisions by the same user not shown)
Line 37: Line 37:
 
         <div class="topnav">
 
         <div class="topnav">
 
           <div class="dropdown">
 
           <div class="dropdown">
−
             <a class="active mainitem" href="#home">HOME</a>
+
             <a class="mainitem" href="https://2020.igem.org/Team:Virginia">HOME</a>
 
             <div class="dropdown-content">
 
             <div class="dropdown-content">
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a>
−
               <a class="hvr-sweep-to-right" href="#abstract">Abstract</a>
+
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia#abstract">Abstract</a>
−
              <a class="hvr-sweep-to-right" href="#problem">Problem</a>
+
−
              <a class="hvr-sweep-to-right" href="#solution">Solution</a>
+
 
             </div>
 
             </div>
 
           </div>
 
           </div>
Line 48: Line 46:
 
             <a class="mainitem" href="#project">PROJECT</a>
 
             <a class="mainitem" href="#project">PROJECT</a>
 
             <div class="dropdown-content">
 
             <div class="dropdown-content">
−
              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Inspiration">Inspiration</a>
 
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a>
Line 54: Line 51:
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Results">Results</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Model">Modeling</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Model">Modeling</a>
−
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Device">Device</a>
+
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Engineering">Engineering</a>
 +
              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Implementation">Implementation</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Safety">Safety</a>
Line 62: Line 60:
 
             <a class="mainitem" href="#parts">PARTS</a>
 
             <a class="mainitem" href="#parts">PARTS</a>
 
             <div class="dropdown-content">
 
             <div class="dropdown-content">
−
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts">New Parts</a>
+
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/New_parts"> Parts</a>
−
              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Utilized_parts">Utilized Parts</a>
+
 
             </div>
 
             </div>
 
           </div>
 
           </div>
Line 73: Line 70:
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Collaborations">Collaborations</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Collaborations">Collaborations</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Contribution">Contributions</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Contribution">Contributions</a>
 +
              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Entrepreneurship">Entrepreneurship</a>
 
             </div>
 
             </div>
 
           </div>
 
           </div>
Line 80: Line 78:
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Attributions">Attributions</a>
−
              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Gallery">Gallery</a>
 
 
             </div>
 
             </div>
 
           </div>
 
           </div>
Line 93: Line 90:
 
             <a class="mainitem" href="#about">RESOURCES</a>
 
             <a class="mainitem" href="#about">RESOURCES</a>
 
             <div class="dropdown-content">
 
             <div class="dropdown-content">
−
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers">Papers</a>
+
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Papers">Sources</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Protocols">Protocols</a>
−
              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Software">Software</a>
 
 
             </div>
 
             </div>
 
           </div>
 
           </div>
Line 125: Line 121:
 
         <div class="section">
 
         <div class="section">
 
           <div class="paragraph">
 
           <div class="paragraph">
−
             Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of MANIFOLD. For more details on how these protocols were utilized, see the <a href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> page. For information on the planned use of these protocols, see the <a href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> page.  
+
             Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the <a href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> page. For information on the planned use of these protocols, see the <a href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> page.  
 
           </div>
 
           </div>
 
         </div>
 
         </div>
Line 168: Line 164:
 
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/d4/T--Virginia--sponsors--UVA.png"/></div>
 
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/d4/T--Virginia--sponsors--UVA.png"/></div>
 
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
 
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/4/41/T--Virginia--images--neb.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/e/e6/T--Virginia--images--twist.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/4/41/T--Virginia--images--cvillbiohub.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/b/b8/T--Virginia--sponsors--addgene.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/7/75/T--Virginia--sponsors--matlab.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/9/96/T--Virginia--sponsors--Benchling.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/6/69/T--Virginia--sponsors--qiagen.png"/></div>
−
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/c/c1/T--Virginia--sponsors--IDT.png"/></div>
+
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/f/f5/T--Virginia--sponsors--lucid.png"/></div>
 
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div>
 
           <div class="sponsoritem hexagon"><img src="https://static.igem.org/mediawiki/2020/d/dc/T--Virginia--sponsors--GenScript.png"/></div>
 
         </div>
 
         </div>

Latest revision as of 03:32, 28 October 2020

Manifold

HOME
Main Abstract
PROJECT
Description Design Experiments Results Modeling Engineering Implementation Notebook Safety
PARTS
Parts
OUTREACH
Human Practices Public Engagement Collaborations Contributions Entrepreneurship
TEAM
Members Attributions
AWARDS
Awards Medals
RESOURCES
Sources Nucleic Acids Protocols
Index:
Lab Notebook
Introduction
Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the Notebook page. For information on the planned use of these protocols, see the Experiments page.
Protocols
Agarose Gel Creation and Pour
Agarose Gel Electrophoresis
Culture from Agar Stab (Inoculation)
DpnI Incubation
Glycerol Stock
Golden Gate Assembly with BsaI
Golden Gate Assembly with BsmBI
NEB Time-Saver qualified Restriction Enzyme Digestion
NEBuilder Hifi Mutagenesis
PCR Purification Kit (GenScript)
Phusion PCR Protocol
QIAGEN HiSpeed Plasmid Purification
QIAprep Spin Miniprep Kit
Restriction Enzyme(s) Digest
Site Directed Mutagenesis
Standard Assembly (Column 3 - pduD + GFP)
Standard Assembly (Combining Column 1 and 3)
Transforming Competent Cells
TRIzol DNA Extraction
FOLLOW US: