Difference between revisions of "Team:Virginia/Protocols"

 
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               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia">Main</a>
 
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               <a class="hvr-sweep-to-right" href="#abstract">Abstract</a>
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             <a class="mainitem" href="#project">PROJECT</a>
 
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              <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Inspiration">Inspiration</a>
 
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Description">Description</a>
 
               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Design">Design</a>
 
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             <a class="mainitem" href="#parts">PARTS</a>
 
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               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/Members">Members</a>
 
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             <a class="mainitem" href="#about">RESOURCES</a>
 
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               <a class="hvr-sweep-to-right" href="https://2020.igem.org/Team:Virginia/nucleic_acids">Nucleic Acids</a>
 
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             Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of MANIFOLD. For more details on how these protocols were utilized, see the <a href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> page. For information on the planned use of these protocols, see the <a href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> page.  
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             Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the <a href="https://2020.igem.org/Team:Virginia/Notebook">Notebook</a> page. For information on the planned use of these protocols, see the <a href="https://2020.igem.org/Team:Virginia/Experiments">Experiments</a> page.  
 
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Latest revision as of 03:32, 28 October 2020

Manifold

HOME
Main Abstract
PROJECT
Description Design Experiments Results Modeling Engineering Implementation Notebook Safety
PARTS
Parts
OUTREACH
Human Practices Public Engagement Collaborations Contributions Entrepreneurship
TEAM
Members Attributions
AWARDS
Awards Medals
RESOURCES
Sources Nucleic Acids Protocols
Index:
Lab Notebook
Introduction
Below are our Benchling Protocol pages that show in detail the protocols we utilized in the construction of Manifold. For more details on how these protocols were utilized, see the Notebook page. For information on the planned use of these protocols, see the Experiments page.
Protocols
Agarose Gel Creation and Pour
Agarose Gel Electrophoresis
Culture from Agar Stab (Inoculation)
DpnI Incubation
Glycerol Stock
Golden Gate Assembly with BsaI
Golden Gate Assembly with BsmBI
NEB Time-Saver qualified Restriction Enzyme Digestion
NEBuilder Hifi Mutagenesis
PCR Purification Kit (GenScript)
Phusion PCR Protocol
QIAGEN HiSpeed Plasmid Purification
QIAprep Spin Miniprep Kit
Restriction Enzyme(s) Digest
Site Directed Mutagenesis
Standard Assembly (Column 3 - pduD + GFP)
Standard Assembly (Combining Column 1 and 3)
Transforming Competent Cells
TRIzol DNA Extraction
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